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Gómez-Letona, Markel; Sebastian, Marta; Montero, María Fernanda; Riebesell, Ulf; Arístegui, Javier (2024): KOSMOS 2018 Gran Canaria mesocosm study: prokaryotes [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.964544

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Published: 2024-01-25DOI registered: 2024-02-23

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Abstract:
This dataset contains the results of the analysis of standing stocks and activity of prokaryotes during the KOSMOS mesocosm experiment carried out in the framework of the Ocean Artificial Upwelling project. The experiment was carried out in the autumn of 2018 in the oligotrophic waters off Gran Canaria. During the 39 days of experiment nutrient-rich deep water was added to the mesocosms in two modes (singular vs recurring additions), with four levels of intensity. Prokaryotic abundances and viability were quantified with a FACSCalibur flow cytometer (Becton-Dickinson). Prokaryotic activity (as protein synthesis) was assessed by means of BioOrthogonal Non-Canonical Amino acid Tagging (BONCAT), using a Zeiss Axio Imager.Z2m Epifluorescence Microscope (AxioCam MRm, Carl Zeiss MicroImaging). The aim of this dataset was to study the effect of different upwelling regimes on the prokaryotic communities and its potential implications for organic matter cycling.
Keyword(s):
Abundance; activity; artificial upwelling; Field experiment; mesocosm experiment; prokaryote; protein; viability
Supplement to:
Gómez-Letona, Markel; Arístegui, Javier; Riebesell, Ulf; Sebastian, Marta (2024): Consistent prokaryotic successional dynamics across contrasting phytoplankton blooms. Limnology and Oceanography, lno.12773, https://doi.org/10.1002/lno.12773
Related to:
Gómez-Letona, Markel; Sebastian, Marta; Baños Cerón, Isabel; Montero, María Fernanda; Pérez Barrancos, Clàudia; Baumann, Moritz; Riebesell, Ulf; Arístegui, Javier (2022): The importance of the dissolved organic matter pool for the carbon sequestration potential of artificial upwelling. Frontiers in Marine Science, 9, 969714, https://doi.org/10.3389/fmars.2022.969714
Ortiz Cortes, Joaquin; Arístegui, Javier; Hernández-Hernández, Nauzet; Fernández-Méndez, Mar; Riebesell, Ulf (2022): Oligotrophic Phytoplankton Community Effectively Adjusts to Artificial Upwelling Regardless of Intensity, but Differently Among Upwelling Modes. Frontiers in Marine Science, 9, 880550, https://doi.org/10.3389/fmars.2022.880550
Project(s):
Funding:
Horizon 2020 (H2020), grant/award no. 695094: Ocean Artificial Upwelling
Coverage:
Median Latitude: 27.927868 * Median Longitude: -15.364305 * South-bound Latitude: 27.927567 * West-bound Longitude: -15.364717 * North-bound Latitude: 27.928167 * East-bound Longitude: -15.363833
Date/Time Start: 2018-11-06T00:00:00 * Date/Time End: 2018-12-14T00:00:00
Event(s):
KOSMOS_2018_Mesocosm-A * Latitude: 27.927883 * Longitude: -15.364333 * Date/Time Start: 2018-11-06T00:00:00 * Date/Time End: 2018-12-14T00:00:00 * Location: Canarias Sea * Campaign: KOSMOS_2018 (KOSMOS Gran Canaria) * Method/Device: Mesocosm experiment (MESO) * Comment: Ambient waters outside the mesocosms
KOSMOS_2018_Mesocosm-DW * Latitude: 27.927883 * Longitude: -15.364333 * Date/Time Start: 2018-11-06T00:00:00 * Date/Time End: 2018-12-14T00:00:00 * Location: Canarias Sea * Campaign: KOSMOS_2018 (KOSMOS Gran Canaria) * Method/Device: Mesocosm experiment (MESO) * Comment: Deep water added to the mesocosms
KOSMOS_2018_Mesocosm-M1 * Latitude: 27.928167 * Longitude: -15.364417 * Date/Time Start: 2018-11-06T00:00:00 * Date/Time End: 2018-12-14T00:00:00 * Location: Canarias Sea * Campaign: KOSMOS_2018 (KOSMOS Gran Canaria) * Method/Device: Mesocosm experiment (MESO)
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Type of studyStudy typeGómez-Letona, Markel
2Event labelEventGómez-Letona, Markel
3DATE/TIMEDate/TimeGómez-Letona, MarkelGeocode
4Day of experimentDOEdayGómez-Letona, Markel
5Mesocosm labelMeso labelGómez-Letona, Markel
6TreatmentTreatGómez-Letona, Markelupwelling mode
7TreatmentTreatGómez-Letona, Markelupwelling intensity
8Treatment: nitrogenT:Nµmol/lGómez-Letona, MarkelInorganic nitrogen introduced to the mesocosms with the deep water additions
9Treatment: nitrogenT:Nµmol/lGómez-Letona, MarkelCumulative inorganic nitrogen introduced to the mesocosms with the deep water additions
10Depth, water, experiment, top/minimumDepth water exp topmGómez-Letona, Markel
11Depth, water, experiment, bottom/maximumDepth water exp maxmGómez-Letona, Markel
12ProkaryotesProkaryotes#/mlGómez-Letona, MarkelFACSCalibur flow-cytometer (Becton Dickinson)samples stained with SYBR Green I
13High nucleic acid prokaryotesHNA prok#/mlGómez-Letona, MarkelFACSCalibur flow-cytometer (Becton Dickinson)samples stained with SYBR Green I
14Low nucleic acid prokaryotesLNA prok#/mlGómez-Letona, MarkelFACSCalibur flow-cytometer (Becton Dickinson)samples stained with SYBR Green I
15High nucleic acid prokaryotes/prokaryotes ratioHNA prok/prokGómez-Letona, MarkelFACSCalibur flow-cytometer (Becton Dickinson)samples stained with SYBR Green I
16ViabilityViab%Gómez-Letona, MarkelFACSCalibur flow-cytometer (Becton Dickinson)of prokaryotes with intact cell membrane; samples stained with SYBR Green I + propidium iodide
17Prokaryotes, activeProkaryotes act%Gómez-Letona, MarkelEpifluorescence Microscope, Carl Zeiss MicroImaging, AxioCam MRm, Zeiss Axio Imager.Z2mof active (protein-synthesising) prokaryotes, BONCAT
18Prokaryotes, active, standard deviationProkaryotes act std dev±Gómez-Letona, MarkelEpifluorescence Microscope, Carl Zeiss MicroImaging, AxioCam MRm, Zeiss Axio Imager.Z2mof BONCAT replicates
19ProchlorococcusProchlorococcus#/mlGómez-Letona, MarkelFACSCalibur flow-cytometer (Becton Dickinson)
20SynechococcusSynechococcus#/mlGómez-Letona, MarkelFACSCalibur flow-cytometer (Becton Dickinson)
21PicoeukaryotesPEuk#/mlGómez-Letona, MarkelFACSCalibur flow-cytometer (Becton Dickinson)
22NanoeukaryotesNEuk#/mlGómez-Letona, MarkelFACSCalibur flow-cytometer (Becton Dickinson)
Change history:
2024-04-03T11:22:17 – The authorship was extended to include additional authors to the first author
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
4394 data points

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