Citation:

PANGAEA.964529

Name: Seawater carbonate chemistry and neuromolnecular responses in disrupted mutualistic cleaning interactions under future environmental conditions
Published: 2024-01-02
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Acanthurus leucosternon; Animalia; Behaviour; Chordata; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Indian Ocean; Laboratory experiment; Labroides dimidiatus; Nekton; Pelagos; Species interaction; Temperature; Tropical

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Abstract:

We evaluated the neuromolecular mechanisms behind the behavioural disruption of cleaning interactions in response to future environments. We subjected cleaner wrasses and surgeonfish (Acanthurus leucosternon, serving as clients) to elevated temperature (warming, 32 °C), increased levels of CO2 (high CO2, 1000 ppm), and a combined condition of elevated CO2 and temperature (warming and high CO2, 32 °C, and 1000 ppm) for 28 days.

Keyword(s):

Acanthurus leucosternon; Animalia; Behaviour; Chordata; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Indian Ocean; Laboratory experiment; Labroides dimidiatus; Nekton; Pelagos; Species interaction; Temperature; Tropical

Supplement to:

Ramírez‑Calero, S; Paula, José Ricardo; Otjacques, Eve; Ravasi, Timothy; Rosa, Rui; Schunter, Celia (2023): Neuromolecular responses in disrupted mutualistic cleaning interactions under future environmental conditions. BMC Biology, 21(1), 258, https://doi.org/10.1186/s12915-023-01761-5

Documentation:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2022): seacarb: seawater carbonate chemistry with R. R package version 3.3.1. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2024-01-02.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type of study	Study type		Schunter, Celia
2	Treatment	Treat		Schunter, Celia
3	Species, unique identification	Species UID		Schunter, Celia
4	Species, unique identification (URI)	Species UID (URI)		Schunter, Celia
5	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		Schunter, Celia
6	Identification	ID		Schunter, Celia		Brain regions dissected: forebrain
7	Identification	ID		Schunter, Celia		Brain regions dissected: midbrain
8	Identification	ID		Schunter, Celia		Brain regions dissected: hindbrain
9	Date	Date		Schunter, Celia		Dissection
10	Fish, standard length	Fish SL	mm	Schunter, Celia
11	Mass	Mass	g	Schunter, Celia
12	Identification	ID		Schunter, Celia
13	Accession number	Access No		Schunter, Celia		Labroides dimidiatus (fore-, mid- and hindbrain)
14	Accession number	Access No		Schunter, Celia		Acanthurus leucosternon (fore-, mid- and hindbrain)
15	Proportion of time	Proportion of time		Schunter, Celia		spent interacting
16	Proportion	Prop		Schunter, Celia		interactions started by cleaners
17	Ratio	Ratio		Schunter, Celia		Client posing display
18	Proportion	Prop		Schunter, Celia		interaction time spent performng tactile stimulation
19	Number	n	#	Schunter, Celia		client jolts per 100s of interaction
20	Duration of interaction	Duration interac	s	Schunter, Celia		mean
21	Temperature, water	Temp	°C	Schunter, Celia
22	Temperature, water, standard deviation	Temp std dev	±	Schunter, Celia
23	pH	pH		Schunter, Celia	Potentiometric	total scale
24	pH, standard deviation	pH std dev	±	Schunter, Celia	Potentiometric	total scale
25	Alkalinity, total	AT	µmol/kg	Schunter, Celia	Spectrophotometric
26	Alkalinity, total, standard deviation	AT std dev	±	Schunter, Celia	Spectrophotometric
27	Salinity	Sal		Schunter, Celia
28	Salinity, standard deviation	Sal std dev	±	Schunter, Celia
29	Carbon, inorganic, dissolved	DIC	µmol/kg	Schunter, Celia	Calculated using CO2SYS
30	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Schunter, Celia	Calculated using CO2SYS
31	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Schunter, Celia	Calculated using CO2SYS
32	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Schunter, Celia	Calculated using CO2SYS
33	Aragonite saturation state	Omega Arg		Schunter, Celia	Calculated using CO2SYS
34	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Schunter, Celia	Calculated using CO2SYS
35	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Carbon dioxide, standard deviation	CO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
38	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	Fugacity of carbon dioxide in seawater, standard deviation	fCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
40	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
42	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
44	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Carbonate ion, standard deviation	[CO3]2- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
46	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
48	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
49	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
50	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
51	Calcite saturation state, standard deviation	Omega Cal std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

3072 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Study type	2 Treat	3 Species UID	4 Species UID (URI)	5 Species UID (Semantic URI)	6 ID (Brain regions dissected: fore...)	7 ID (Brain regions dissected: midb...)	8 ID (Brain regions dissected: hind...)	9 Date (Dissection)	10 Fish SL [mm]	11 Mass [g]	12 ID	13 Access No (Labroides dimidiatus (fore-, ...)	14 Access No (Acanthurus leucosternon (fore...)	15 Proportion of time (spent interacting)	16 Prop (interactions started by cleaners)	17 Ratio (Client posing display)	18 Prop (interaction time spent perfor...)	19 n [#] (client jolts per 100s of inte...)	20 Duration interac [s] (mean)	21 Temp [°C]	22 Temp std dev [±]	23 pH (total scale, Potentiometric)	24 pH std dev [±] (total scale, Potentiometric)	25 AT [µmol/kg] (Spectrophotometric)	26 AT std dev [±] (Spectrophotometric)	27 Sal	28 Sal std dev [±]	29 DIC [µmol/kg] (Calculated using CO2SYS)	30 DIC std dev [±] (Calculated using CO2SYS)	31 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	32 pCO2 std dev [±] (Calculated using CO2SYS)	33 Omega Arg (Calculated using CO2SYS)	34 Omega Arg std dev [±] (Calculated using CO2SYS)	35 CSC flag (Calculated using seacarb afte...)	36 CO2 [µmol/kg] (Calculated using seacarb afte...)	37 CO2 std dev [±] (Calculated using seacarb afte...)	38 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	39 fCO2 std dev [±] (Calculated using seacarb afte...)	40 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	41 pCO2 std dev [±] (Calculated using seacarb afte...)	42 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	43 [HCO3]- std dev [±] (Calculated using seacarb afte...)	44 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	45 [CO3]2- std dev [±] (Calculated using seacarb afte...)	46 DIC [µmol/kg] (Calculated using seacarb afte...)	47 DIC std dev [±] (Calculated using seacarb afte...)	48 Omega Arg (Calculated using seacarb afte...)	49 Omega Arg std dev [±] (Calculated using seacarb afte...)	50 Omega Cal (Calculated using seacarb afte...)	51 Omega Cal std dev [±] (Calculated using seacarb afte...)
laboratory	Control	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	LD13FB	LD13MB	LD13HB	2023-08-09T10:15:00	63.0	2.77										29.3	0.8	8.09	0.09	2503.5	268.8	35.6	0.7	2112.3	219.0	397.0	97.6	4.6	0.9	8	9.51	2.75	373.06	107.44	374.20	107.76	1811.38	229.98	285.68	53.84	2106.57	246.36	4.61	0.87	6.90	1.30
laboratory	Control	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	LD14FB	LD14MB	LD14HB	2023-08-09T10:15:00	54.0	1.92										29.3	0.8	8.09	0.09	2503.5	268.8	35.6	0.7	2112.3	219.0	397.0	97.6	4.6	0.9	8	9.51	2.75	373.06	107.44	374.20	107.76	1811.38	229.98	285.68	53.84	2106.57	246.36	4.61	0.87	6.90	1.30
laboratory	Control	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	LD23FB	LD23MB	LD23HB	2023-08-11T09:30:00	60.0	2.30										29.3	0.8	8.09	0.09	2503.5	268.8	35.6	0.7	2112.3	219.0	397.0	97.6	4.6	0.9	8	9.51	2.75	373.06	107.44	374.20	107.76	1811.38	229.98	285.68	53.84	2106.57	246.36	4.61	0.87	6.90	1.30
laboratory	Control	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	LD24FB	LD24MB	LD24HB	2023-08-11T09:30:00	61.0	2.82										29.3	0.8	8.09	0.09	2503.5	268.8	35.6	0.7	2112.3	219.0	397.0	97.6	4.6	0.9	8	9.51	2.75	373.06	107.44	374.20	107.76	1811.38	229.98	285.68	53.84	2106.57	246.36	4.61	0.87	6.90	1.30
laboratory	Control	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	LD3FB	LD3MB	LD3HB	2023-08-12T09:30:00	57.0	1.92										29.3	0.8	8.09	0.09	2503.5	268.8	35.6	0.7	2112.3	219.0	397.0	97.6	4.6	0.9	8	9.51	2.75	373.06	107.44	374.20	107.76	1811.38	229.98	285.68	53.84	2106.57	246.36	4.61	0.87	6.90	1.30
laboratory	Control	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	LD4FB	LD4MB	LD4HB	2023-08-12T09:30:00	51.0	1.57										29.3	0.8	8.09	0.09	2503.5	268.8	35.6	0.7	2112.3	219.0	397.0	97.6	4.6	0.9	8	9.51	2.75	373.06	107.44	374.20	107.76	1811.38	229.98	285.68	53.84	2106.57	246.36	4.61	0.87	6.90	1.30
laboratory	Control	Acanthurus leucosternon	marinespecies.org	urn:lsid:marinespecies.org:taxname:219628	AL37FB	AL37MB	AL37HB	2023-08-07T09:30:00	74.0	16.98										29.3	0.8	8.09	0.09	2503.5	268.8	35.6	0.7	2112.3	219.0	397.0	97.6	4.6	0.9	8	9.51	2.75	373.06	107.44	374.20	107.76	1811.38	229.98	285.68	53.84	2106.57	246.36	4.61	0.87	6.90	1.30
laboratory	Control	Acanthurus leucosternon	marinespecies.org	urn:lsid:marinespecies.org:taxname:219628	AL38FB	AL38MB	AL38HB	2023-08-07T09:30:00	75.0	17.06										29.3	0.8	8.09	0.09	2503.5	268.8	35.6	0.7	2112.3	219.0	397.0	97.6	4.6	0.9	8	9.51	2.75	373.06	107.44	374.20	107.76	1811.38	229.98	285.68	53.84	2106.57	246.36	4.61	0.87	6.90	1.30
laboratory	Control	Acanthurus leucosternon	marinespecies.org	urn:lsid:marinespecies.org:taxname:219628	AL47FB	AL47MB	AL47HB	2023-08-08T09:30:00	72.0	15.96										29.3	0.8	8.09	0.09	2503.5	268.8	35.6	0.7	2112.3	219.0	397.0	97.6	4.6	0.9	8	9.51	2.75	373.06	107.44	374.20	107.76	1811.38	229.98	285.68	53.84	2106.57	246.36	4.61	0.87	6.90	1.30
laboratory	Control	Acanthurus leucosternon	marinespecies.org	urn:lsid:marinespecies.org:taxname:219628	AL50FB	AL50MB	AL50HB	2023-08-08T09:30:00	105.0	52.24										29.3	0.8	8.09	0.09	2503.5	268.8	35.6	0.7	2112.3	219.0	397.0	97.6	4.6	0.9	8	9.51	2.75	373.06	107.44	374.20	107.76	1811.38	229.98	285.68	53.84	2106.57	246.36	4.61	0.87	6.90	1.30
laboratory	Control	Acanthurus leucosternon	marinespecies.org	urn:lsid:marinespecies.org:taxname:219628	AL57FB	AL57MB	AL57HB	2023-08-09T08:45:00	72.0	17.15										29.3	0.8	8.09	0.09	2503.5	268.8	35.6	0.7	2112.3	219.0	397.0	97.6	4.6	0.9	8	9.51	2.75	373.06	107.44	374.20	107.76	1811.38	229.98	285.68	53.84	2106.57	246.36	4.61	0.87	6.90	1.30
laboratory	Control	Acanthurus leucosternon	marinespecies.org	urn:lsid:marinespecies.org:taxname:219628	AL60FB	AL60MB	AL60HB	2023-08-09T09:30:00	105.0	56.52										29.3	0.8	8.09	0.09	2503.5	268.8	35.6	0.7	2112.3	219.0	397.0	97.6	4.6	0.9	8	9.51	2.75	373.06	107.44	374.20	107.76	1811.38	229.98	285.68	53.84	2106.57	246.36	4.61	0.87	6.90	1.30
laboratory	High CO2	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	LD11FB	LD11MB	LD11HB	2023-08-09T09:30:00	60.0	2.26										29.5	0.9	7.73	0.09	2516.4	351.9	35.5	0.6	2319.3	335.8	1004.2	230.2	2.6	0.5	8	25.88	7.19	1019.06	282.20	1022.15	283.05	2156.92	315.85	149.23	34.30	2332.03	337.13	2.41	0.56	3.61	0.83
laboratory	High CO2	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	LD12FB	LD12MB	LD12HB	2023-08-09T10:15:00	79.3	2.30										29.5	0.9	7.73	0.09	2516.4	351.9	35.5	0.6	2319.3	335.8	1004.2	230.2	2.6	0.5	8	25.88	7.19	1019.06	282.20	1022.15	283.05	2156.92	315.85	149.23	34.30	2332.03	337.13	2.41	0.56	3.61	0.83
laboratory	High CO2	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	LD1FB	LD1MB	LD1HB	2023-08-10T10:15:00	60.0	2.61										29.5	0.9	7.73	0.09	2516.4	351.9	35.5	0.6	2319.3	335.8	1004.2	230.2	2.6	0.5	8	25.88	7.19	1019.06	282.20	1022.15	283.05	2156.92	315.85	149.23	34.30	2332.03	337.13	2.41	0.56	3.61	0.83
laboratory	High CO2	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	LD21FB	LD21MB	LD21HB	2023-08-11T08:45:00	77.0	2.81										29.5	0.9	7.73	0.09	2516.4	351.9	35.5	0.6	2319.3	335.8	1004.2	230.2	2.6	0.5	8	25.88	7.19	1019.06	282.20	1022.15	283.05	2156.92	315.85	149.23	34.30	2332.03	337.13	2.41	0.56	3.61	0.83
laboratory	High CO2	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	LD22FB	LD22MB	LD22HB	2023-08-11T08:45:00	72.0	2.17										29.5	0.9	7.73	0.09	2516.4	351.9	35.5	0.6	2319.3	335.8	1004.2	230.2	2.6	0.5	8	25.88	7.19	1019.06	282.20	1022.15	283.05	2156.92	315.85	149.23	34.30	2332.03	337.13	2.41	0.56	3.61	0.83
laboratory	High CO2	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	LD2FB	LD2MB	LD2HB	2023-08-12T08:45:00	68.0	1.90										29.5	0.9	7.73	0.09	2516.4	351.9	35.5	0.6	2319.3	335.8	1004.2	230.2	2.6	0.5	8	25.88	7.19	1019.06	282.20	1022.15	283.05	2156.92	315.85	149.23	34.30	2332.03	337.13	2.41	0.56	3.61	0.83
laboratory	High CO2	Acanthurus leucosternon	marinespecies.org	urn:lsid:marinespecies.org:taxname:219628	AL35FB	AL35MB	AL35HB	2023-08-07T09:30:00	103.0	47.60										29.5	0.9	7.73	0.09	2516.4	351.9	35.5	0.6	2319.3	335.8	1004.2	230.2	2.6	0.5	8	25.88	7.19	1019.06	282.20	1022.15	283.05	2156.92	315.85	149.23	34.30	2332.03	337.13	2.41	0.56	3.61	0.83
laboratory	High CO2	Acanthurus leucosternon	marinespecies.org	urn:lsid:marinespecies.org:taxname:219628	AL36FB	AL36MB	AL36HB	2023-08-07T09:30:00	137.0	57.36										29.5	0.9	7.73	0.09	2516.4	351.9	35.5	0.6	2319.3	335.8	1004.2	230.2	2.6	0.5	8	25.88	7.19	1019.06	282.20	1022.15	283.05	2156.92	315.85	149.23	34.30	2332.03	337.13	2.41	0.56	3.61	0.83
laboratory	High CO2	Acanthurus leucosternon	marinespecies.org	urn:lsid:marinespecies.org:taxname:219628	AL45FB	AL45MB	AL45HB	2023-08-08T08:45:00	107.0	58.19										29.5	0.9	7.73	0.09	2516.4	351.9	35.5	0.6	2319.3	335.8	1004.2	230.2	2.6	0.5	8	25.88	7.19	1019.06	282.20	1022.15	283.05	2156.92	315.85	149.23	34.30	2332.03	337.13	2.41	0.56	3.61	0.83
laboratory	High CO2	Acanthurus leucosternon	marinespecies.org	urn:lsid:marinespecies.org:taxname:219628	AL46FB	AL46MB	AL46HB	2023-08-08T08:45:00	126.0	33.95										29.5	0.9	7.73	0.09	2516.4	351.9	35.5	0.6	2319.3	335.8	1004.2	230.2	2.6	0.5	8	25.88	7.19	1019.06	282.20	1022.15	283.05	2156.92	315.85	149.23	34.30	2332.03	337.13	2.41	0.56	3.61	0.83
laboratory	High CO2	Acanthurus leucosternon	marinespecies.org	urn:lsid:marinespecies.org:taxname:219628	AL55FB	AL55MB	AL55HB	2023-08-09T08:45:00	139.0	52.87										29.5	0.9	7.73	0.09	2516.4	351.9	35.5	0.6	2319.3	335.8	1004.2	230.2	2.6	0.5	8	25.88	7.19	1019.06	282.20	1022.15	283.05	2156.92	315.85	149.23	34.30	2332.03	337.13	2.41	0.56	3.61	0.83
laboratory	High CO2	Acanthurus leucosternon	marinespecies.org	urn:lsid:marinespecies.org:taxname:219628	AL56FB	AL56MB	AL56HB	2023-08-09T08:45:00	134.0	47.29										29.5	0.9	7.73	0.09	2516.4	351.9	35.5	0.6	2319.3	335.8	1004.2	230.2	2.6	0.5	8	25.88	7.19	1019.06	282.20	1022.15	283.05	2156.92	315.85	149.23	34.30	2332.03	337.13	2.41	0.56	3.61	0.83
laboratory	Warming	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	LD17FB	LD17MB	LD17HB	2023-08-10T08:45:00	19.1	6.60										32.1	0.3	8.04	0.08	2431.2	265.8	35.4	0.6	2075.6	233.2	487.3	108.2	4.2	0.9	8	9.94	2.60	414.61	108.46	415.83	108.78	1766.70	222.02	273.26	48.16	2049.90	242.04	4.49	0.79	6.65	1.17
laboratory	Warming	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	LD18FB	LD18MB	LD18HB	2023-08-10T09:30:00	14.9	5.80										32.1	0.3	8.04	0.08	2431.2	265.8	35.4	0.6	2075.6	233.2	487.3	108.2	4.2	0.9	8	9.94	2.60	414.61	108.46	415.83	108.78	1766.70	222.02	273.26	48.16	2049.90	242.04	4.49	0.79	6.65	1.17
laboratory	Warming	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	LD27FB	LD27MB	LD27HB	2023-08-12T08:45:00	16.1	6.80										32.1	0.3	8.04	0.08	2431.2	265.8	35.4	0.6	2075.6	233.2	487.3	108.2	4.2	0.9	8	9.94	2.60	414.61	108.46	415.83	108.78	1766.70	222.02	273.26	48.16	2049.90	242.04	4.49	0.79	6.65	1.17
laboratory	Warming	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	LD28FB	LD28MB	LD28HB	2023-08-12T08:45:00	17.1	6.30										32.1	0.3	8.04	0.08	2431.2	265.8	35.4	0.6	2075.6	233.2	487.3	108.2	4.2	0.9	8	9.94	2.60	414.61	108.46	415.83	108.78	1766.70	222.02	273.26	48.16	2049.90	242.04	4.49	0.79	6.65	1.17
laboratory	Warming	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	LD7FB	LD7MB	LD7HB	2023-08-15T10:15:00	21.8	7.00										32.1	0.3	8.04	0.08	2431.2	265.8	35.4	0.6	2075.6	233.2	487.3	108.2	4.2	0.9	8	9.94	2.60	414.61	108.46	415.83	108.78	1766.70	222.02	273.26	48.16	2049.90	242.04	4.49	0.79	6.65	1.17
laboratory	Warming	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	LD8FB	LD8MB	LD8HB	2023-08-16T10:15:00	27.1	7.30										32.1	0.3	8.04	0.08	2431.2	265.8	35.4	0.6	2075.6	233.2	487.3	108.2	4.2	0.9	8	9.94	2.60	414.61	108.46	415.83	108.78	1766.70	222.02	273.26	48.16	2049.90	242.04	4.49	0.79	6.65	1.17
laboratory	Warming	Acanthurus leucosternon	marinespecies.org	urn:lsid:marinespecies.org:taxname:219628	AL33FB	AL33MB	AL33HB	2023-08-07T08:45:00	254.6	11.40										32.1	0.3	8.04	0.08	2431.2	265.8	35.4	0.6	2075.6	233.2	487.3	108.2	4.2	0.9	8	9.94	2.60	414.61	108.46	415.83	108.78	1766.70	222.02	273.26	48.16	2049.90	242.04	4.49	0.79	6.65	1.17
laboratory	Warming	Acanthurus leucosternon	marinespecies.org	urn:lsid:marinespecies.org:taxname:219628	AL34FB	AL34MB	AL34HB	2023-08-07T08:45:00	147.3	9.70										32.1	0.3	8.04	0.08	2431.2	265.8	35.4	0.6	2075.6	233.2	487.3	108.2	4.2	0.9	8	9.94	2.60	414.61	108.46	415.83	108.78	1766.70	222.02	273.26	48.16	2049.90	242.04	4.49	0.79	6.65	1.17
laboratory	Warming	Acanthurus leucosternon	marinespecies.org	urn:lsid:marinespecies.org:taxname:219628	AL43FB	AL43MB	AL43HB	2023-08-08T08:45:00	280.0	11.70										32.1	0.3	8.04	0.08	2431.2	265.8	35.4	0.6	2075.6	233.2	487.3	108.2	4.2	0.9	8	9.94	2.60	414.61	108.46	415.83	108.78	1766.70	222.02	273.26	48.16	2049.90	242.04	4.49	0.79	6.65	1.17
laboratory	Warming	Acanthurus leucosternon	marinespecies.org	urn:lsid:marinespecies.org:taxname:219628	AL44FB	AL44MB	AL44HB	2023-08-08T08:45:00	226.0	11.10										32.1	0.3	8.04	0.08	2431.2	265.8	35.4	0.6	2075.6	233.2	487.3	108.2	4.2	0.9	8	9.94	2.60	414.61	108.46	415.83	108.78	1766.70	222.02	273.26	48.16	2049.90	242.04	4.49	0.79	6.65	1.17
laboratory	Warming	Acanthurus leucosternon	marinespecies.org	urn:lsid:marinespecies.org:taxname:219628	AL53FB	AL53MB	AL53HB	2023-08-08T10:15:00	240.2	11.00										32.1	0.3	8.04	0.08	2431.2	265.8	35.4	0.6	2075.6	233.2	487.3	108.2	4.2	0.9	8	9.94	2.60	414.61	108.46	415.83	108.78	1766.70	222.02	273.26	48.16	2049.90	242.04	4.49	0.79	6.65	1.17
laboratory	Warming	Acanthurus leucosternon	marinespecies.org	urn:lsid:marinespecies.org:taxname:219628	AL54FB	AL54MB	AL54HB	2023-08-08T10:15:00	229.5	11.10										32.1	0.3	8.04	0.08	2431.2	265.8	35.4	0.6	2075.6	233.2	487.3	108.2	4.2	0.9	8	9.94	2.60	414.61	108.46	415.83	108.78	1766.70	222.02	273.26	48.16	2049.90	242.04	4.49	0.79	6.65	1.17
laboratory	Warming & High CO2	Acanthurus leucosternon	marinespecies.org	urn:lsid:marinespecies.org:taxname:219628	AL52FB	AL52MB	AL52HB	2023-08-08T10:15:00	94.0	37.59										31.9	0.4	7.70	0.10	2477.7	231.2	35.6	0.7	2286.8	215.7	1065.8	195.3	2.5	0.5	8	26.10	7.33	1085.37	304.67	1088.57	305.57	2117.42	214.82	149.31	32.70	2292.83	224.78	2.45	0.54	3.63	0.79
laboratory	Warming & High CO2	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	LD10FB	LD10MB	LD10HB	2023-08-09T09:30:00	60.0	2.40										31.9	0.4	7.70	0.10	2477.7	231.2	35.6	0.7	2286.8	215.7	1065.8	195.3	2.5	0.5	8	26.10	7.33	1085.37	304.67	1088.57	305.57	2117.42	214.82	149.31	32.70	2292.83	224.78	2.45	0.54	3.63	0.79
laboratory	Warming & High CO2	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	LD19FB	LD19MB	LD19HB	2023-08-10T09:30:00	60.0	2.26										31.9	0.4	7.70	0.10	2477.7	231.2	35.6	0.7	2286.8	215.7	1065.8	195.3	2.5	0.5	8	26.10	7.33	1085.37	304.67	1088.57	305.57	2117.42	214.82	149.31	32.70	2292.83	224.78	2.45	0.54	3.63	0.79
laboratory	Warming & High CO2	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	LD20FB	LD20MB	LD20HB	2023-08-10T10:15:00	55.0	1.84										31.9	0.4	7.70	0.10	2477.7	231.2	35.6	0.7	2286.8	215.7	1065.8	195.3	2.5	0.5	8	26.10	7.33	1085.37	304.67	1088.57	305.57	2117.42	214.82	149.31	32.70	2292.83	224.78	2.45	0.54	3.63	0.79
laboratory	Warming & High CO2	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	LD29FB	LD29MB	LD29HB	2023-08-12T08:45:00	60.0	2.59										31.9	0.4	7.70	0.10	2477.7	231.2	35.6	0.7	2286.8	215.7	1065.8	195.3	2.5	0.5	8	26.10	7.33	1085.37	304.67	1088.57	305.57	2117.42	214.82	149.31	32.70	2292.83	224.78	2.45	0.54	3.63	0.79
laboratory	Warming & High CO2	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	LD30FB	LD30MB	LD30HB	2023-08-12T09:30:00	52.0	1.46										31.9	0.4	7.70	0.10	2477.7	231.2	35.6	0.7	2286.8	215.7	1065.8	195.3	2.5	0.5	8	26.10	7.33	1085.37	304.67	1088.57	305.57	2117.42	214.82	149.31	32.70	2292.83	224.78	2.45	0.54	3.63	0.79
laboratory	Warming & High CO2	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	LD9FB	LD9MB	LD9HB	2023-08-17T10:15:00	57.0	1.89										31.9	0.4	7.70	0.10	2477.7	231.2	35.6	0.7	2286.8	215.7	1065.8	195.3	2.5	0.5	8	26.10	7.33	1085.37	304.67	1088.57	305.57	2117.42	214.82	149.31	32.70	2292.83	224.78	2.45	0.54	3.63	0.79
laboratory	Warming & High CO2	Acanthurus leucosternon	marinespecies.org	urn:lsid:marinespecies.org:taxname:219628	AL31FB	AL31MB	AL31HB	2023-08-07T08:45:00	106.0	49.15										31.9	0.4	7.70	0.10	2477.7	231.2	35.6	0.7	2286.8	215.7	1065.8	195.3	2.5	0.5	8	26.10	7.33	1085.37	304.67	1088.57	305.57	2117.42	214.82	149.31	32.70	2292.83	224.78	2.45	0.54	3.63	0.79
laboratory	Warming & High CO2	Acanthurus leucosternon	marinespecies.org	urn:lsid:marinespecies.org:taxname:219628	AL32FB	AL32MB	AL32HB	2023-08-07T08:45:00	92.0	33.47										31.9	0.4	7.70	0.10	2477.7	231.2	35.6	0.7	2286.8	215.7	1065.8	195.3	2.5	0.5	8	26.10	7.33	1085.37	304.67	1088.57	305.57	2117.42	214.82	149.31	32.70	2292.83	224.78	2.45	0.54	3.63	0.79
laboratory	Warming & High CO2	Acanthurus leucosternon	marinespecies.org	urn:lsid:marinespecies.org:taxname:219628	AL41FB	AL41MB	AL41HB	2023-08-07T10:15:00	105.0	51.32										31.9	0.4	7.70	0.10	2477.7	231.2	35.6	0.7	2286.8	215.7	1065.8	195.3	2.5	0.5	8	26.10	7.33	1085.37	304.67	1088.57	305.57	2117.42	214.82	149.31	32.70	2292.83	224.78	2.45	0.54	3.63	0.79
laboratory	Warming & High CO2	Acanthurus leucosternon	marinespecies.org	urn:lsid:marinespecies.org:taxname:219628	AL42FB	AL42MB	AL42HB	2023-08-07T10:15:00	92.0	33.23										31.9	0.4	7.70	0.10	2477.7	231.2	35.6	0.7	2286.8	215.7	1065.8	195.3	2.5	0.5	8	26.10	7.33	1085.37	304.67	1088.57	305.57	2117.42	214.82	149.31	32.70	2292.83	224.78	2.45	0.54	3.63	0.79
laboratory	Warming & High CO2	Acanthurus leucosternon	marinespecies.org	urn:lsid:marinespecies.org:taxname:219628	AL51FB	AL51MB	AL51HB	2023-08-08T10:15:00	93.0	32.03										31.9	0.4	7.70	0.10	2477.7	231.2	35.6	0.7	2286.8	215.7	1065.8	195.3	2.5	0.5	8	26.10	7.33	1085.37	304.67	1088.57	305.57	2117.42	214.82	149.31	32.70	2292.83	224.78	2.45	0.54	3.63	0.79
laboratory	High CO2										LD01AL35	SRR14415212	SRR14415126	0.025	0.688	0.009	0.000	0.000	10.661	29.5	0.9	7.73	0.09	2516.4	351.9	35.5	0.6	2319.3	335.8	1004.2	230.2	2.6	0.5	8	25.88	7.19	1019.06	282.20	1022.15	283.05	2156.92	315.85	149.23	34.30	2332.03	337.13	2.41	0.56	3.61	0.83
laboratory												SRR14415211	SRR14415125
laboratory												SRR14415039	SRR14415124
laboratory	High CO2										LD02AL36	SRR14415205	SRR14415123	0.131	0.042	0.026	0.021	0.000	20.151	29.5	0.9	7.73	0.09	2516.4	351.9	35.5	0.6	2319.3	335.8	1004.2	230.2	2.6	0.5	8	25.88	7.19	1019.06	282.20	1022.15	283.05	2156.92	315.85	149.23	34.30	2332.03	337.13	2.41	0.56	3.61	0.83
laboratory												SRR14415194	SRR14415122
laboratory												SRR14415183	SRR14415121
laboratory	Control										LD03AL38	SRR14415172	SRR14415116	0.184	0.580	0.027	0.000	0.219	18.879	29.3	0.8	8.09	0.09	2503.5	268.8	35.6	0.7	2112.3	219.0	397.0	97.6	4.6	0.9	8	9.51	2.75	373.06	107.44	374.20	107.76	1811.38	229.98	285.68	53.84	2106.57	246.36	4.61	0.87	6.90	1.30
laboratory												SRR14415161	SRR14415115
laboratory												SRR14415150	SRR14415114
laboratory	Control										LD04AL37	SRR14415139	SRR14415120	0.179	0.587	0.019	0.009	2.635	46.061	29.3	0.8	8.09	0.09	2503.5	268.8	35.6	0.7	2112.3	219.0	397.0	97.6	4.6	0.9	8	9.51	2.75	373.06	107.44	374.20	107.76	1811.38	229.98	285.68	53.84	2106.57	246.36	4.61	0.87	6.90	1.30
laboratory												SRR14415210	SRR14415119
laboratory												SRR14415118	SRR14415117
laboratory	Warming										LD07AL33	SRR14415041	SRR14415132	0.005	0.000	0.059	0.000	0.000	4.754	32.1	0.3	8.04	0.08	2431.2	265.8	35.4	0.6	2075.6	233.2	487.3	108.2	4.2	0.9	8	9.94	2.60	414.61	108.46	415.83	108.78	1766.70	222.02	273.26	48.16	2049.90	242.04	4.49	0.79	6.65	1.17
laboratory												SRR14415040	SRR14415131
laboratory												SRR14415038	SRR14415130
laboratory	Warming										LD08AL34	SRR14415037	SRR14415129	0.002	0.000	0.025	0.000	0.000	5.401	32.1	0.3	8.04	0.08	2431.2	265.8	35.4	0.6	2075.6	233.2	487.3	108.2	4.2	0.9	8	9.94	2.60	414.61	108.46	415.83	108.78	1766.70	222.02	273.26	48.16	2049.90	242.04	4.49	0.79	6.65	1.17
laboratory												SRR14415036	SRR14415128
laboratory												SRR14415035	SRR14415127
laboratory	Warming & High CO2										LD09AL31	SRR14415034	SRR14415138	0.167	0.045	0.041	0.015	1.449	9.224	31.9	0.4	7.70	0.10	2477.7	231.2	35.6	0.7	2286.8	215.7	1065.8	195.3	2.5	0.5	8	26.10	7.33	1085.37	304.67	1088.57	305.57	2117.42	214.82	149.31	32.70	2292.83	224.78	2.45	0.54	3.63	0.79
laboratory												SRR14415033	SRR14415137
laboratory												SRR14415209	SRR14415136
laboratory	Warming & High CO2										LD10AL32	SRR14415208	SRR14415135	0.014	0.000	0.010	0.000	0.000	4.384	31.9	0.4	7.70	0.10	2477.7	231.2	35.6	0.7	2286.8	215.7	1065.8	195.3	2.5	0.5	8	26.10	7.33	1085.37	304.67	1088.57	305.57	2117.42	214.82	149.31	32.70	2292.83	224.78	2.45	0.54	3.63	0.79
laboratory												SRR14415207	SRR14415134
laboratory												SRR14415206	SRR14415133
laboratory	High CO2										LD11AL45	SRR14415204	SRR14415093	0.047	0.048	0.012	0.001	1.519	10.891	29.5	0.9	7.73	0.09	2516.4	351.9	35.5	0.6	2319.3	335.8	1004.2	230.2	2.6	0.5	8	25.88	7.19	1019.06	282.20	1022.15	283.05	2156.92	315.85	149.23	34.30	2332.03	337.13	2.41	0.56	3.61	0.83
laboratory												SRR14415203	SRR14415092
laboratory												SRR14415202	SRR14415091
laboratory	High CO2										LD12AL46	SRR14415201	SRR14415090	0.082	0.053	0.027	0.009	0.000	15.269	29.5	0.9	7.73	0.09	2516.4	351.9	35.5	0.6	2319.3	335.8	1004.2	230.2	2.6	0.5	8	25.88	7.19	1019.06	282.20	1022.15	283.05	2156.92	315.85	149.23	34.30	2332.03	337.13	2.41	0.56	3.61	0.83
laboratory												SRR14415200	SRR14415089
laboratory												SRR14415199	SRR14415088
laboratory	Control										LD13AL50	SRR14415198	SRR14415077	0.210	0.938	0.003	0.002	0.580	24.664	29.3	0.8	8.09	0.09	2503.5	268.8	35.6	0.7	2112.3	219.0	397.0	97.6	4.6	0.9	8	9.51	2.75	373.06	107.44	374.20	107.76	1811.38	229.98	285.68	53.84	2106.57	246.36	4.61	0.87	6.90	1.30
laboratory												SRR14415197	SRR14415076
laboratory												SRR14415196	SRR14415075
laboratory	Control										LD14AL47	SRR14415195	SRR14415087	0.079	0.824	0.004	0.000	0.000	14.380	29.3	0.8	8.09	0.09	2503.5	268.8	35.6	0.7	2112.3	219.0	397.0	97.6	4.6	0.9	8	9.51	2.75	373.06	107.44	374.20	107.76	1811.38	229.98	285.68	53.84	2106.57	246.36	4.61	0.87	6.90	1.30
laboratory												SRR14415193	SRR14415086
laboratory												SRR14415192	SRR14415084
laboratory	Warming										LD17AL43	SRR14415185	SRR14415100	0.007	0.000	0.044	0.000	0.000	7.712	32.1	0.3	8.04	0.08	2431.2	265.8	35.4	0.6	2075.6	233.2	487.3	108.2	4.2	0.9	8	9.94	2.60	414.61	108.46	415.83	108.78	1766.70	222.02	273.26	48.16	2049.90	242.04	4.49	0.79	6.65	1.17
laboratory												SRR14415184	SRR14415099
laboratory												SRR14415182	SRR14415098
laboratory	Warming										LD18AL44	SRR14415181	SRR14415097	0.057	0.162	0.031	0.001	0.765	8.051	32.1	0.3	8.04	0.08	2431.2	265.8	35.4	0.6	2075.6	233.2	487.3	108.2	4.2	0.9	8	9.94	2.60	414.61	108.46	415.83	108.78	1766.70	222.02	273.26	48.16	2049.90	242.04	4.49	0.79	6.65	1.17
laboratory												SRR14415180	SRR14415095
laboratory												SRR14415179	SRR14415094
laboratory	Warming & High CO2										LD19AL41	SRR14415178	SRR14415106	0.026	0.083	0.040	0.000	1.368	9.477	31.9	0.4	7.70	0.10	2477.7	231.2	35.6	0.7	2286.8	215.7	1065.8	195.3	2.5	0.5	8	26.10	7.33	1085.37	304.67	1088.57	305.57	2117.42	214.82	149.31	32.70	2292.83	224.78	2.45	0.54	3.63	0.79
laboratory												SRR14415177	SRR14415105
laboratory												SRR14415176	SRR14415104
laboratory	Warming & High CO2										LD20AL42	SRR14415175	SRR14415103	0.024	0.294	0.047	0.004	0.000	5.369	31.9	0.4	7.70	0.10	2477.7	231.2	35.6	0.7	2286.8	215.7	1065.8	195.3	2.5	0.5	8	26.10	7.33	1085.37	304.67	1088.57	305.57	2117.42	214.82	149.31	32.70	2292.83	224.78	2.45	0.54	3.63	0.79
laboratory												SRR14415174	SRR14415102
laboratory												SRR14415173	SRR14415101
laboratory	High CO2										LD21AL55	SRR14415171	SRR14415060	0.007	0.000	0.014	0.000	0.000	6.421	29.5	0.9	7.73	0.09	2516.4	351.9	35.5	0.6	2319.3	335.8	1004.2	230.2	2.6	0.5	8	25.88	7.19	1019.06	282.20	1022.15	283.05	2156.92	315.85	149.23	34.30	2332.03	337.13	2.41	0.56	3.61	0.83
laboratory												SRR14415170	SRR14415059
laboratory												SRR14415169	SRR14415058
laboratory	High CO2										LD22AL56	SRR14415168	SRR14415057	0.002	0.000	0.022	0.000	0.000	1.218	29.5	0.9	7.73	0.09	2516.4	351.9	35.5	0.6	2319.3	335.8	1004.2	230.2	2.6	0.5	8	25.88	7.19	1019.06	282.20	1022.15	283.05	2156.92	315.85	149.23	34.30	2332.03	337.13	2.41	0.56	3.61	0.83
laboratory												SRR14415167	SRR14415056
laboratory												SRR14415166	SRR14415055
laboratory	Control										LD23AL60	SRR14415165	SRR14415044	0.111	0.500	0.008	0.017	0.748	24.226	29.3	0.8	8.09	0.09	2503.5	268.8	35.6	0.7	2112.3	219.0	397.0	97.6	4.6	0.9	8	9.51	2.75	373.06	107.44	374.20	107.76	1811.38	229.98	285.68	53.84	2106.57	246.36	4.61	0.87	6.90	1.30
laboratory												SRR14415164	SRR14415043
laboratory												SRR14415163	SRR14415042
laboratory	Control										LD24AL57	SRR14415162	SRR14415054	0.112	0.880	0.003	0.000	0.618	11.472	29.3	0.8	8.09	0.09	2503.5	268.8	35.6	0.7	2112.3	219.0	397.0	97.6	4.6	0.9	8	9.51	2.75	373.06	107.44	374.20	107.76	1811.38	229.98	285.68	53.84	2106.57	246.36	4.61	0.87	6.90	1.30
laboratory												SRR14415160	SRR14415053
laboratory												SRR14415159	SRR14415051
laboratory	Warming										LD27AL53	SRR14415152	SRR14415067	0.007	0.000	0.030	0.000	0.000	3.827	32.1	0.3	8.04	0.08	2431.2	265.8	35.4	0.6	2075.6	233.2	487.3	108.2	4.2	0.9	8	9.94	2.60	414.61	108.46	415.83	108.78	1766.70	222.02	273.26	48.16	2049.90	242.04	4.49	0.79	6.65	1.17
laboratory												SRR14415151	SRR14415066
laboratory												SRR14415149	SRR14415065
laboratory	Warming										LD28AL54	SRR14415148	SRR14415070	0.136	0.157	0.032	0.002	0.547	8.504	32.1	0.3	8.04	0.08	2431.2	265.8	35.4	0.6	2075.6	233.2	487.3	108.2	4.2	0.9	8	9.94	2.60	414.61	108.46	415.83	108.78	1766.70	222.02	273.26	48.16	2049.90	242.04	4.49	0.79	6.65	1.17
laboratory												SRR14415147	SRR14415069
laboratory												SRR14415146	SRR14415068
laboratory	Warming & High CO2										LD29AL51	SRR14415145	SRR14415073	0.197	0.158	0.035	0.010	0.770	10.226	31.9	0.4	7.70	0.10	2477.7	231.2	35.6	0.7	2286.8	215.7	1065.8	195.3	2.5	0.5	8	26.10	7.33	1085.37	304.67	1088.57	305.57	2117.42	214.82	149.31	32.70	2292.83	224.78	2.45	0.54	3.63	0.79
laboratory												SRR14415144	SRR14415072
laboratory												SRR14415143	SRR14415071
laboratory	Warming & High CO2										LD30AL52	SRR14415142	SRR14415070	0.149	0.304	0.020	0.000	1.946	8.720	31.9	0.4	7.70	0.10	2477.7	231.2	35.6	0.7	2286.8	215.7	1065.8	195.3	2.5	0.5	8	26.10	7.33	1085.37	304.67	1088.57	305.57	2117.42	214.82	149.31	32.70	2292.83	224.78	2.45	0.54	3.63	0.79
laboratory												SRR14415141	SRR14415069
laboratory												SRR14415140	SRR14415068