Citation:

PANGAEA.963276

Name: Evolutionary Adaptation of Protein Turnover in White Muscle of Stenothermal Antarctic Fish: Elevated Cold Compensation at Reduced Thermal Responsiveness
Published: 2023-11-21
License: https://creativecommons.org/licenses/by/4.0/
Keywords: 13C-labeling; acute warming; fish physiology; metabolic profiling; NMR; protein degradation; protein synthesis rate

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Abstract:

Protein turnover is highly energy consuming and overall relates to an organism's growth performance varying largely between species, e.g., due to pre-adaptation to environmental characteristics such as temperature. Here, we determined protein synthesis rates and capacity of protein degradation in white muscle of the cold stenothermal Antarctic eelpout (Pachycara brachycephalum) and its closely related temperate counterpart, the eurythermal common eelpout (Zoarces viviparus). Both species were exposed to acute warming (P. brachycephalum, 0 °C + 2 °C/day; Z. viviparus, 4 °C + 3 °C/day). The in vivo protein synthesis rate (Ks) was monitored after injection of 13C-phenylalanine, and protein degradation capacity was quantified by measuring the activity of cathepsin D in vitro. Untargeted metabolic profiling by nuclear magnetic resonance (NMR) spectroscopy was used to identify the metabolic processes involved. Independent of temperature, the protein synthesis rate was higher in P. brachycephalum (Ks = 0.38–0.614 %/day) than in Z. viviparus (Ks= 0.148-0.379%/day). Whereas protein synthesis remained unaffected by temperature in the Antarctic species, protein synthesis in Z. viviparus increased to near the thermal optimum (16 °C) and tended to fall at higher temperatures. Most strikingly, capacities for protein degradation were about ten times higher in the Antarctic compared to the temperate species. These differences are mirrored in the metabolic profiles, with significantly higher levels of complex and essential amino acids in the free cytosolic pool of the Antarctic congener. Together, the results clearly indicate a highly cold-compensated protein turnover in the Antarctic eelpout compared to its temperate confamilial. Constant versus variable environments are mirrored in rigid versus plastic functional responses of the protein synthesis machinery.

Keyword(s):

13C-labeling; acute warming; fish physiology; metabolic profiling; NMR; protein degradation; protein synthesis rate

Supplement to:

Krebs, Nina; Bock, Christian; Tebben, Jan; Mark, Felix Christopher; Lucassen, Magnus; Lannig, Gisela; Pörtner, Hans-Otto (2023): Evolutionary Adaptation of Protein Turnover in White Muscle of Stenothermal Antarctic Fish: Elevated Cold Compensation at Reduced Thermal Responsiveness. Biomolecules, 13(10), 1507, https://doi.org/10.3390/biom13101507

Coverage:

Latitude: 53.533000 * Longitude: 8.580100

Event(s):

AWI_lab * Latitude: 53.533000 * Longitude: 8.580100 * Location: Bremerhaven, Germany * Method/Device: Experiment (EXP)

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Sample code/label	Sample label		Krebs, Nina
2	Sample code/label 2	Sample label 2		Krebs, Nina
3	Species	Species		Krebs, Nina
4	Treatment	Treat		Krebs, Nina		Code
5	Treatment	Treat		Krebs, Nina		Method
6	Date/time start, experiment	Date/time start exp		Krebs, Nina
7	Sampling date/time, experiment	Date/time sampling exp		Krebs, Nina
8	Time in hours	Time	h	Krebs, Nina		Injection Time
9	Temperature, technical	T tech	°C	Krebs, Nina		sampling T
10	Pachycara brachycephalum, standard length	P. brachycephalum SL	cm	Krebs, Nina	Tape measure
11	Pachycara brachycephalum, mass	P. brachycephalum m	g	Krebs, Nina	Scale
12	Pachycara brachycephalum, liver, mass	P. brachycephalum liver m	g	Krebs, Nina	Scale
13	Gender	Gender		Krebs, Nina		male m or female f; state [between 1 (no gonads) and 5 (spawning)]
14	Proteins, synthesis rate, per day	Protein synthesis	%/day	Krebs, Nina
15	Cahepsin D activity per protein mass	Cahepsin D act/prot	U/mg	Krebs, Nina
16	tau-Methylhistidine	tau-Methylhistidine	mmol/l	Krebs, Nina	1H NMR spectroscopy
17	pi-Methylhistidine	pi-Methylhistidine	mmol/l	Krebs, Nina	1H NMR spectroscopy
18	beta-Alanine	b-ala	mmol/l	Krebs, Nina	1H NMR spectroscopy
19	Valine	Val	mmol/l	Krebs, Nina	1H NMR spectroscopy
20	Tyrosine	Tyr	mmol/l	Krebs, Nina	1H NMR spectroscopy
21	Tryptophan	Trp	mmol/l	Krebs, Nina	1H NMR spectroscopy
22	Trimethylamine N-oxide	TMAO	mmol/l	Krebs, Nina	1H NMR spectroscopy
23	Trimethylamine	TMA	mmol/l	Krebs, Nina	1H NMR spectroscopy
24	Threonine	Thr	mmol/l	Krebs, Nina	1H NMR spectroscopy
25	Taurine	Tau	mmol/l	Krebs, Nina	1H NMR spectroscopy
26	Succinate	Succinate	mmol/l	Krebs, Nina	1H NMR spectroscopy
27	Serine	Ser	mmol/l	Krebs, Nina	1H NMR spectroscopy
28	Sarcosine	Sarcosine	mmol/l	Krebs, Nina	1H NMR spectroscopy
29	Phenylglyoxylate	Phenylglyoxylate	mmol/l	Krebs, Nina	1H NMR spectroscopy
30	O-Phosphocholine	O-Phosphocholine	mmol/l	Krebs, Nina	1H NMR spectroscopy
31	O-Acetylcholine	O-Acetylcholine	mmol/l	Krebs, Nina	1H NMR spectroscopy
32	O-Acetylcarnitine	O-Acetylcarnitine	mmol/l	Krebs, Nina	1H NMR spectroscopy
33	N,N-Dimethylglycine	NN-Dimethylglycine	mmol/l	Krebs, Nina	1H NMR spectroscopy
34	Methionine	Methionine	mmol/l	Krebs, Nina	1H NMR spectroscopy
35	Lysine	Lys	mmol/l	Krebs, Nina	1H NMR spectroscopy
36	Leucine	Leu	mmol/l	Krebs, Nina	1H NMR spectroscopy
37	Lactate	Lactate	mmol/l	Krebs, Nina	1H NMR spectroscopy
38	Isoleucine	Ile	mmol/l	Krebs, Nina	1H NMR spectroscopy
39	Inosine	Inosine	mmol/l	Krebs, Nina	1H NMR spectroscopy
40	Inosine monophosphate	IMP	mmol/l	Krebs, Nina	1H NMR spectroscopy
41	Hypotaurine	Hypotaurine	mmol/l	Krebs, Nina	1H NMR spectroscopy
42	Homocysteine	Homocysteine	mmol/l	Krebs, Nina	1H NMR spectroscopy
43	Histidine	His	mmol/l	Krebs, Nina	1H NMR spectroscopy
44	Histamine	Histamine	mmol/l	Krebs, Nina	1H NMR spectroscopy
45	Glycine	Gly	mmol/l	Krebs, Nina	1H NMR spectroscopy
46	Glutamine	Gln	mmol/l	Krebs, Nina	1H NMR spectroscopy
47	Glutamate	Glutamate	mmol/l	Krebs, Nina	1H NMR spectroscopy
48	Glucose-1-phosphate	G1P	mmol/l	Krebs, Nina	1H NMR spectroscopy
49	Fumarate	Fumarate	mmol/l	Krebs, Nina	1H NMR spectroscopy
50	Dimethylamine	DMA	mmol/l	Krebs, Nina	1H NMR spectroscopy
51	Creatine phosphate	Creatine phosphate	mmol/l	Krebs, Nina	1H NMR spectroscopy
52	Creatine	Creatine	mmol/l	Krebs, Nina	1H NMR spectroscopy
53	Citraconate	Citraconate	mmol/l	Krebs, Nina	1H NMR spectroscopy
54	Choline	Choline	mmol/l	Krebs, Nina	1H NMR spectroscopy
55	Betaine	Betaine	mmol/l	Krebs, Nina	1H NMR spectroscopy
56	Aspartate	Aspartate	mmol/l	Krebs, Nina	1H NMR spectroscopy
57	Asparagine	Asn	mmol/l	Krebs, Nina	1H NMR spectroscopy
58	Alanine	Ala	mmol/l	Krebs, Nina	1H NMR spectroscopy
59	Acetate	Acetate	mmol/l	Krebs, Nina	1H NMR spectroscopy
60	Adenylates, total	Adenylates	mmol/l	Krebs, Nina	1H NMR spectroscopy

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

3235 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Sample label	2 Sample label 2	3 Species	4 Treat (Code)	5 Treat (Method)	6 Date/time start exp	7 Date/time sampling exp	8 Time [h] (Injection Time)	9 T tech [°C] (sampling T)	10 P. brachycephalum SL [cm] (Tape measure)	11 P. brachycephalum m [g] (Scale)	12 P. brachycephalum liver m [g] (Scale)	13 Gender (male m or female f; state [be...)	14 Protein synthesis [%/day]	15 Cahepsin D act/prot [U/mg]	16 tau-Methylhistidine [mmol/l] (1H NMR spectroscopy)	17 pi-Methylhistidine [mmol/l] (1H NMR spectroscopy)	18 b-ala [mmol/l] (1H NMR spectroscopy)	19 Val [mmol/l] (1H NMR spectroscopy)	20 Tyr [mmol/l] (1H NMR spectroscopy)	21 Trp [mmol/l] (1H NMR spectroscopy)	22 TMAO [mmol/l] (1H NMR spectroscopy)	23 TMA [mmol/l] (1H NMR spectroscopy)	24 Thr [mmol/l] (1H NMR spectroscopy)	25 Tau [mmol/l] (1H NMR spectroscopy)	26 Succinate [mmol/l] (1H NMR spectroscopy)	27 Ser [mmol/l] (1H NMR spectroscopy)	28 Sarcosine [mmol/l] (1H NMR spectroscopy)	29 Phenylglyoxylate [mmol/l] (1H NMR spectroscopy)	30 O-Phosphocholine [mmol/l] (1H NMR spectroscopy)	31 O-Acetylcholine [mmol/l] (1H NMR spectroscopy)	32 O-Acetylcarnitine [mmol/l] (1H NMR spectroscopy)	33 NN-Dimethylglycine [mmol/l] (1H NMR spectroscopy)	34 Methionine [mmol/l] (1H NMR spectroscopy)	35 Lys [mmol/l] (1H NMR spectroscopy)	36 Leu [mmol/l] (1H NMR spectroscopy)	37 Lactate [mmol/l] (1H NMR spectroscopy)	38 Ile [mmol/l] (1H NMR spectroscopy)	39 Inosine [mmol/l] (1H NMR spectroscopy)	40 IMP [mmol/l] (1H NMR spectroscopy)	41 Hypotaurine [mmol/l] (1H NMR spectroscopy)	42 Homocysteine [mmol/l] (1H NMR spectroscopy)	43 His [mmol/l] (1H NMR spectroscopy)	44 Histamine [mmol/l] (1H NMR spectroscopy)	45 Gly [mmol/l] (1H NMR spectroscopy)	46 Gln [mmol/l] (1H NMR spectroscopy)	47 Glutamate [mmol/l] (1H NMR spectroscopy)	48 G1P [mmol/l] (1H NMR spectroscopy)	49 Fumarate [mmol/l] (1H NMR spectroscopy)	50 DMA [mmol/l] (1H NMR spectroscopy)	51 Creatine phosphate [mmol/l] (1H NMR spectroscopy)	52 Creatine [mmol/l] (1H NMR spectroscopy)	53 Citraconate [mmol/l] (1H NMR spectroscopy)	54 Choline [mmol/l] (1H NMR spectroscopy)	55 Betaine [mmol/l] (1H NMR spectroscopy)	56 Aspartate [mmol/l] (1H NMR spectroscopy)	57 Asn [mmol/l] (1H NMR spectroscopy)	58 Ala [mmol/l] (1H NMR spectroscopy)	59 Acetate [mmol/l] (1H NMR spectroscopy)	60 Adenylates [mmol/l] (1H NMR spectroscopy)
Pb_44	Pb_0_1.5_1	Pachycara brachycephalum	Pb_0_1.5h	Injection of 13C PHE + sampling	2020-06-14	2020-06-15	1.5	0	22.0	58.5	1.090	m/2		1.337665	0.0162	0.0170	0.0323	1.6356	0.5143	0.0975	19.5721	0.0109	0.8778	4.2837	0.0907	1.1815	0.1433	0.0255	0.0870	0.0323	0.0175	0.0625	0.3703	2.3930	1.3749	8.0401	1.0208	0.0778	0.7747	0.0007	0.1509	0.4879	0.0259	1.8324	0.2964	0.8316	0.1507	0.0167	0.1464	1.1776	11.2099	0.0200	0.0615	0.5279	0.0472	0.4840	1.0655	0.1460	0.6545
Pb_45	Pb_0_3_1	Pachycara brachycephalum	Pb_0_3h	Injection of 13C PHE + sampling	2020-06-14	2020-06-15	3.0	0	21.5	37.5	1.520	m/2	0.623048350	1.174700	0.0199	0.0483	0.0272	2.0506	0.2514	0.1184	24.9928	0.0098	1.0547	4.7159	0.0808	0.9220	0.0904	0.0437	0.0932	0.0567	0.0505	0.0144	0.5210	2.0836	1.7767	9.3487	1.3733	0.1011	0.8995	0.0008	0.1574	0.4750	0.0409	2.8172	0.3322	0.5706	0.1561	0.0161	0.0914	1.2774	12.6314	0.0278	0.0565	0.3326	0.0558	0.3396	0.8920	0.1899	0.9420
Pb_46	Pb_2_1.5_1	Pachycara brachycephalum	Pb_2_1.5h	Injection of 13C PHE + sampling	2020-06-14	2020-06-16	1.5	2	20.0	37.6	0.890	m/2	0.518987099
Pb_47	Pb_2_3_1	Pachycara brachycephalum	Pb_2_3h	Injection of 13C PHE + sampling	2020-06-14	2020-06-16	3.0	2	22.5	43.3	1.160	f/2	0.306776998
Pb_48	Pb_4_1.5_1	Pachycara brachycephalum	Pb_4_1.5h	Injection of 13C PHE + sampling	2020-06-14	2020-06-17	1.5	4	23.0	47.5		m/2	0.581317752	0.563685	0.0147	0.0631	0.0532	1.0240	0.1232	0.0542	16.3050	0.0114	0.9773	5.5728	0.1265	1.4991	0.0700	0.0334	0.1023	0.0362	0.0426	0.0723	0.2546	1.3300	0.8511	8.1763	0.6655	0.0291	0.5851	0.0011	0.1569	0.5382	0.0235	2.4800	0.2740	0.8452	0.1204	0.0124	0.0702	1.2474	9.5719	0.0265	0.0642	0.5083	0.0711	0.7432	1.3390	0.1120	0.7946
Pb_49	Pb_4_3_1	Pachycara brachycephalum	Pb_4_3h	Injection of 13C PHE + sampling	2020-06-14	2020-06-17	3.0	4	23.0	48.2	1.200	m/2	0.375976984	0.893862	0.0237	0.0483	0.0185	1.9334	0.4646	0.1482	24.4910	0.0082	1.5006	3.2734	0.0555	0.9316	0.0822	0.0277	0.0708	0.0534	0.0292	0.0262	0.6252	2.1584	1.6408	8.2689	1.2449	0.0288	0.4408	0.0006	0.0611	0.4653	0.0251	3.0306	0.2752	0.4602	0.1076	0.0097	0.0873	2.5294	11.1754	0.0272	0.0580	0.3826	0.0439	0.6280	1.0028	0.0906	1.1734
Pb_50	Pb_6_1.5_1	Pachycara brachycephalum	Pb_6_1.5h	Injection of 13C PHE + sampling	2020-06-14	2020-06-18	1.5	6	26.0	60.0	1.443	f/2-5	0.628061417
Pb_51	Pb_6_3_1	Pachycara brachycephalum	Pb_6_3h	Injection of 13C PHE + sampling	2020-06-14	2020-06-18	3.0	6	23.5	50.3	1.616	f/2	0.505327160
Pb_52	Pb_8_1.5_1	Pachycara brachycephalum	Pb_8_1.5h	Injection of 13C PHE + sampling	2020-06-14	2020-06-19	1.5	8	21.0	36.7	1.220	f/2	0.391216287
Pb_53	Pb_8_3_1	Pachycara brachycephalum	Pb_8_3h	Injection of 13C PHE + sampling	2020-06-14	2020-06-19	3.0	8	23.0	47.5	1.010	f/2	0.329778321
Pb_54	Pb_10_1.5_1	Pachycara brachycephalum	Pb_10_1.5h	Injection of 13C PHE + sampling	2020-06-14	2020-06-20	1.5	10	23.0	35.4	0.940	f2	0.426545030	1.605189	0.0224	0.0540	0.0253	1.7677	0.5359	0.1316	21.7139	0.0102	0.8667	2.9641	0.0797	1.1435	0.1691	0.0277	0.1021	0.0234	0.0406	0.0228	0.4504	1.8040	1.3138	11.1258	0.9953	0.0489	0.9277	0.0005	0.1307	0.4952	0.0327	3.2119	0.2752	0.4988	0.1277	0.0200	0.1568	0.8036	10.7671	0.0226	0.0841	0.5220	0.0558	0.9213	0.7717	0.0982	0.5766
Pb_55	Pb_10_3_1	Pachycara brachycephalum	Pb_10_3h	Injection of 13C PHE + sampling	2020-06-14	2020-06-20	3.0	10	22.0	42.4	1.210	f/2	0.347407712	1.361547	0.0140	0.0455	0.0302	0.6701	0.2619	0.0872	18.4676	0.0141	0.8718	6.1678	0.0978	1.3853	0.0930	0.0270	0.1314	0.0257	0.0275	0.0978	0.3166	1.3576	0.4925	7.0116	0.3749	0.0327	0.4408	0.0008	0.1215	0.5527	0.0369	3.6818	0.3413	0.8755	0.1205	0.0168	0.0975	2.5972	10.2045	0.0216	0.0853	0.3058	0.0378	0.7467	1.2187	0.0953	1.1734
Pb_56	Pb_0_1.5_2	Pachycara brachycephalum	Pb_0_1.5h	Injection of 13C PHE + sampling	2020-06-21	2020-06-22	1.5	0	20.5	32.0	0.708	m/2	0.359974862		0.0186	0.0455	0.0297	2.4021	0.5004	0.1185	25.7661	0.0091	1.5617	3.0343	0.0447	0.8730	0.0816	0.0356	0.0865	0.0484	0.0148	0.0135	0.5964	3.0863	1.9181	5.1777	1.4084	0.0200	0.6970	0.0006	0.1574	0.5343	0.0208	1.6677	0.3768	0.4062	0.2177	0.0119	0.0446	1.1291	12.5693	0.0245	0.0412	0.4448	0.0887	0.5526	0.6017	0.1129	0.7941
Pb_57	Pb_0_3_2	Pachycara brachycephalum	Pb_0_3h	Injection of 13C PHE + sampling	2020-06-21	2020-06-22	3.0	0	24.0	49.7	2.104	m/2	0.184208496	2.262626	0.0239	0.0450	0.0316	2.4145	0.4865	0.1610	22.2396	0.0109	1.2701	4.5494	0.0902	1.2502	0.0887	0.0457	0.0963	0.0686	0.0456	0.0135	0.3958	3.2621	2.1076	10.5282	1.7422	0.0383	0.7697	0.0007	0.1661	0.5043	0.0160	3.4734	0.3768	0.5935	0.1442	0.0183	0.0964	0.7472	12.6221	0.0285	0.0481	0.3422	0.0887	0.6000	0.8926	0.1019	0.9036
Pb_58	Pb_2_1.5_2	Pachycara brachycephalum	Pb_2_1.5h	Injection of 13C PHE + sampling	2020-06-21	2020-06-23	1.5	2	21.0	33.7	0.700	f/2	0.707965285
Pb_59	Pb_2_3_2	Pachycara brachycephalum	Pb_2_3h	Injection of 13C PHE + sampling	2020-06-21	2020-06-23	3.0	2	22.0	52.5	1.210	m/2	0.181966476
Pb_60	Pb_4_1.5_2	Pachycara brachycephalum	Pb_4_1.5h	Injection of 13C PHE + sampling	2020-06-21	2020-06-24	1.5	4	26.0	53.3	0.750	m/2-3	0.381980409	0.800734	0.0123	0.0477	0.0499	0.4373	0.2119	0.0862	30.9684	0.0138	0.6185	3.7579	0.0649	0.9351	0.0267	0.0548	0.0739	0.0315	0.0641	0.0120	0.1944	0.8499	0.4549	11.3432	0.2736	0.0352	0.8031	0.0006	0.1894	0.1398	0.0229	1.2449	0.4038	0.2760	0.1771	0.0142	0.0273	2.0636	14.9022	0.0419	0.1162	0.2485	0.0581	0.3559	1.2160	0.1071	1.0558
Pb_61	Pb_4_3_2	Pachycara brachycephalum	Pb_4_3h	Injection of 13C PHE + sampling	2020-06-21	2020-06-24	3.0	4	26.0	68.9	1.420	f/4		1.553327	0.0225	0.0551	0.0277	1.5549	0.3449	0.0977	25.0764	0.0120	0.9696	2.8401	0.0686	0.9943	0.0733	0.0521	0.1113	0.0890	0.0637	0.0129	0.4825	2.5781	1.3859	9.0663	1.1042	0.0381	0.7338	0.0005	0.1927	0.4384	0.0160	1.6023	0.2496	0.7444	0.2172	0.0175	0.0794	2.0148	12.3211	0.0522	0.1144	0.3644	0.0201	0.5350	0.7539	0.1050	0.9478
Pb_62	Pb_6_1.5_2	Pachycara brachycephalum	Pb_6_1.5h	Injection of 13C PHE + sampling	2020-06-21	2020-06-25	1.5	6	22.0	40.6	0.950	f/2	0.357244213
Pb_63	Pb_6_3_2	Pachycara brachycephalum	Pb_6_3h	Injection of 13C PHE + sampling	2020-06-21	2020-06-25	3.0	6	24.0	46.2	1.080	m/2	0.130441507
Pb_64	Pb_8_1.5_2	Pachycara brachycephalum	Pb_8_1.5h	Injection of 13C PHE + sampling	2020-06-21	2020-06-26	1.5	8	21.0	43.0	1.260	f/2	0.220354187
Pb_65	Pb_8_3_2	Pachycara brachycephalum	Pb_8_3h	Injection of 13C PHE + sampling	2020-06-21	2020-06-26	3.0	8	20.5	32.0	0.960	m/1	0.759818042
Pb_66	Pb_10_1.5_2	Pachycara brachycephalum	Pb_10_1.5h	Injection of 13C PHE + sampling	2020-06-21	2020-06-27	1.5	10	23.5	48.9	1.450	m/2	0.922351517	2.870391
Pb_67	Pb_10_3_2	Pachycara brachycephalum	Pb_10_3h	Injection of 13C PHE + sampling	2020-06-21	2020-06-27	3.0	10	21.5	31.9	0.500	f/2	0.161701456	3.235830	0.0154	0.0565	0.0348	1.3392	0.5013	0.1565	25.4556	0.0132	1.1420	3.7671	0.0612	1.1348	0.0617	0.0359	0.1063	0.0181	0.0352	0.0416	0.4062	2.1807	1.1494	7.0204	0.8510	0.0335	0.8894	0.0006	0.1658	0.4890	0.0289	2.9325	0.2949	0.5085	0.1618	0.0107	0.0609	2.0243	12.0762	0.0524	0.0774	0.4305	0.0232	0.4291	0.7612	0.0978	0.9527
Pb_68	Pb_0_1.5_3	Pachycara brachycephalum	Pb_0_1.5h	Injection of 13C PHE + sampling	2020-06-28	2020-06-29	1.5	0	25.5	64.0	0.986	f/4	0.915088871	0.861967	0.0195	0.0323	0.0321	0.1432	0.0420	0.0370	28.4520	0.0121	0.3868	2.4326	0.0512	0.5806	0.0376	0.0371	0.1610	0.0512	0.0662	0.0123	0.0625	0.5597	0.1558	8.0673	0.1005	0.1211	0.5561	0.0002	0.0777	0.0490	0.0095	0.8851	0.2027	0.2410	0.1553	0.0083	0.0442	0.7096	13.2883	0.0242	0.1142	0.2110	0.0357	0.1060	0.7612	0.1263	0.5912
Pb_69	Pb_0_3_3	Pachycara brachycephalum	Pb_0_3h	Injection of 13C PHE + sampling	2020-06-28	2020-06-29	3.0	0	22.0	40.1	1.103	f/2	0.298920686	0.894131	0.0182	0.0509	0.0569	0.7026	0.2281	0.0739	21.5745	0.0142	0.8684	3.9734	0.0546	0.6970	0.0743	0.0384	0.1115	0.0447	0.0235	0.0049	0.1705	1.4775	0.6421	8.5383	0.4577	0.0456	0.5388	0.0009	0.0933	0.1974	0.0153	1.5949	0.2823	0.4655	0.1085	0.0102	0.0463	1.1512	10.6943	0.0499	0.0375	0.2457	0.0752	0.2219	0.5744	0.1518	0.7651
Pb_70	Pb_2_1.5_3	Pachycara brachycephalum	Pb_2_1.5h	Injection of 13C PHE + sampling	2020-06-28	2020-06-30	1.5	2	21.0	37.4	0.880	m/1-2	1.434519582
Pb_71	Pb_2_3_3	Pachycara brachycephalum	Pb_2_3h	Injection of 13C PHE + sampling	2020-06-28	2020-06-30	3.0	2	24.0	46.3	1.370	m/2	0.521634832
Pb_72	Pb_4_1.5_3	Pachycara brachycephalum	Pb_4_1.5h	Injection of 13C PHE + sampling	2020-06-28	2020-07-01	1.5	4	21.5	36.2	0.920	m/2	0.650497505	2.960127	0.0180	0.0416	0.0674	1.0460	0.2205	0.0935	24.7745	0.0146	0.8285	4.5922	0.0673	0.7573	0.0968	0.0298	0.1101	0.0441	0.0396	0.0175	0.3873	2.6052	0.6978	7.4920	0.5897	0.0623	0.6211	0.0011	0.1295	0.2212	0.0313	1.2787	0.2552	0.5277	0.1678	0.0145	0.0580	1.5360	12.2138	0.0416	0.0630	0.4558	0.1094	0.3161	0.4055	0.2065	0.7920
Pb_73	Pb_4_3_3	Pachycara brachycephalum	Pb_4_3h	Injection of 13C PHE + sampling	2020-06-28	2020-07-01	3.0	4	21.5	35.9	1.050	m/2	0.331678777	0.631975	0.0154	0.0569	0.0355	1.4576	0.4039	0.1755	23.7080	0.0146	0.8002	3.0303	0.0877	1.1931	0.1996	0.0481	0.1211	0.0362	0.0339	0.0372	0.2286	1.7793	1.1812	11.0126	0.8471	0.0257	0.9667	0.0015	0.0851	0.4923	0.0204	3.5619	0.2763	0.5320	0.1495	0.0167	0.1876	1.0936	12.4159	0.0724	0.0977	0.6654	0.0897	0.3058	1.4827	0.2181	0.7594
Pb_74	Pb_6_1.5_3	Pachycara brachycephalum	Pb_6_1.5h	Injection of 13C PHE + sampling	2020-06-28	2020-07-02	1.5	6	20.5	32.3	0.920	f/2	0.467967408
Pb_75	Pb_6_3_3	Pachycara brachycephalum	Pb_6_3h	Injection of 13C PHE + sampling	2020-06-28	2020-07-02	3.0	6	23.5	54.8	1.580	m/2	0.229711057
Pb_76	Pb_8_1.5_3	Pachycara brachycephalum	Pb_8_1.5h	Injection of 13C PHE + sampling	2020-06-28	2020-07-03	1.5	8	19.0	26.2	0.900	m/1-2	0.475866457
Pb_77	Pb_8_3_3	Pachycara brachycephalum	Pb_8_3h	Injection of 13C PHE + sampling	2020-06-28	2020-07-03	3.0	8	26.0	56.3		f/2	0.523280568
Pb_79	Pb_10_3_3	Pachycara brachycephalum	Pb_10_3h	Injection of 13C PHE + sampling	2020-06-28	2020-07-04	3.0	10	20.0	31.3	1.140	m/2	0.295714498	1.444473	0.0149	0.0666	0.0475	1.8612	0.2852	0.1164	21.3711	0.0188	0.5314	3.8744	0.0776	1.0851	0.2176	0.0453	0.1179	0.0284	0.0322	0.0314	0.1748	1.6741	1.2580	6.4021	1.0421	0.0714	0.7797	0.0005	0.2217	0.3388	0.0262	1.8245	0.2763	1.3202	0.2180	0.0169	0.1640	1.0756	11.1948	0.0490	0.0874	0.3910	0.0897	0.8911	1.4002	0.2186	0.5770
Pb_80	Pb_0_1.5_4	Pachycara brachycephalum	Pb_0_1.5h	Injection of 13C PHE + sampling	2020-07-05	2020-07-06	1.5	0	24.0	45.0	1.420	m/2	0.454456412	1.910689	0.0145	0.0420	0.0366	1.4403	0.2931	0.1077	19.9321	0.0171	0.6945	3.9607	0.0556	0.7162	0.0641	0.0453	0.0885	0.0423	0.0294	0.0057	0.3015	2.0289	1.1734	7.6676	0.9878	0.0507	0.6827	0.0008	0.1578	0.3314	0.0216	2.2401	0.2763	0.6097	0.1617	0.0211	0.0567	0.6331	10.3087	0.0303	0.0629	0.3038	0.1069	0.8223	1.7656	0.1179	0.6859
Pb_81	Pb_0_3_4	Pachycara brachycephalum	Pb_0_3h	Injection of 13C PHE + sampling	2020-07-05	2020-07-06	3.0	0	24.5	56.4	1.650	f/3-4	0.452591449	2.198669	0.0208	0.0346	0.0250	0.9226	0.2695	0.0969	22.5604	0.0206	1.0091	4.6246	0.0912	0.9728	0.0528	0.0288	0.2138	0.0276	0.0521	0.0129	0.2450	1.3392	1.0782	7.3759	0.6930	0.0218	0.6421	0.0012	0.1994	0.3483	0.0239	1.7633	0.3505	0.6442	0.1976	0.0188	0.0346	1.5473	11.1529	0.0181	0.0462	0.4449	0.1002	0.6709	1.8257	0.1219	1.1124
Pb_82	Pb_2_1.5_4	Pachycara brachycephalum	Pb_2_1.5h	Injection of 13C PHE + sampling	2020-07-05	2020-07-07	1.5	2	24.0	44.7	1.980	f/2	1.100309279
Pb_83	Pb_2_3_4	Pachycara brachycephalum	Pb_2_3h	Injection of 13C PHE + sampling	2020-07-05	2020-07-07	3.0	2	27.5	60.5	0.900	m/2	0.142982656
Pb_84	Pb_4_1.5_4	Pachycara brachycephalum	Pb_4_1.5h	Injection of 13C PHE + sampling	2020-07-05	2020-07-08	1.5	4	25.5	54.7	1.380	m/2	0.755149453	2.047237	0.0108	0.0420	0.0437	0.3107	0.1341	0.0579	19.7385	0.0153	0.5277	3.9017	0.0543	0.8548	0.0926	0.0312	0.0921	0.0478	0.0377	0.0073	0.1382	0.8359	0.2645	7.8988	0.1804	0.0825	0.6247	0.0005	0.1787	0.1410	0.0157	1.7642	0.2763	0.4814	0.1147	0.0135	0.0481	0.9963	9.2964	0.0404	0.0850	0.4208	0.0495	0.3165	1.6604	0.0728	0.6489
Pb_85	Pb_4_3_4	Pachycara brachycephalum	Pb_4_3h	Injection of 13C PHE + sampling	2020-07-05	2020-07-08	3.0	4	23.5	53.7	2.360	m/2	0.210551697	0.771665	0.0173	0.0354	0.0190	1.1201	0.2975	0.1557	24.5732	0.0158	0.6126	3.5125	0.0634	0.8908	0.1243	0.0462	0.0639	0.0228	0.0434	0.0353	0.2498	1.2147	0.9953	10.7409	0.7712	0.0393	0.9832	0.0005	0.1943	0.4015	0.0177	2.7804	0.2763	0.5716	0.1104	0.0188	0.1166	1.9827	12.4182	0.0295	0.0651	0.4922	0.0293	0.6381	1.3134	0.0921	1.0671
Pb_86	Pb_6_1.5_4	Pachycara brachycephalum	Pb_6_1.5h	Injection of 13C PHE + sampling	2020-07-05	2020-07-09	1.5	6	23.0	49.4	1.310	m/2	0.704005652
Pb_87	Pb_6_3_4	Pachycara brachycephalum	Pb_6_3h	Injection of 13C PHE + sampling	2020-07-05	2020-07-09	3.0	6	20.5	36.0	1.316	m/2	0.179134515
Pb_88	Pb_8_1.5_4	Pachycara brachycephalum	Pb_8_1.5h	Injection of 13C PHE + sampling	2020-07-05	2020-07-10	1.5	8	21.0	30.2	0.940	f/2	0.250390028
Pb_89	Pb_8_3_4	Pachycara brachycephalum	Pb_8_3h	Injection of 13C PHE + sampling	2020-07-05	2020-07-10	3.0	8	22.5	37.4	0.900	m/2	0.344236105
Pb_90	Pb_10_1.5_4	Pachycara brachycephalum	Pb_10_1.5h	Injection of 13C PHE + sampling	2020-07-05	2020-07-11	1.5	10	24.0	41.8	1.100	f/2	0.307012432	1.221602	0.0173	0.0381	0.0396	0.7930	0.2975	0.0930	25.6210	0.0153	0.5727	3.8344	0.0910	0.8728	0.0522	0.0451	0.0998	0.0293	0.0483	0.0249	0.3544	1.6174	0.6978	8.5541	0.5229	0.0259	0.8322	0.0008	0.1960	0.3457	0.0324	2.3177	0.2763	0.4118	0.1905	0.0117	0.0349	1.1634	12.2127	0.0304	0.1308	0.3505	0.0487	0.5222	1.3134	0.1078	0.9245
Pb_91	Pb_10_3_4	Pachycara brachycephalum	Pb_10_3h	Injection of 13C PHE + sampling	2020-07-05	2020-07-11	3.0	10	25.0	52.5	1.330	m/2	0.195954355	1.989116	0.0098	0.0381	0.0341	0.2328	0.1564	0.0702	21.8129	0.0182	0.4605	4.0193	0.0587	0.9555	0.0547	0.0363	0.1294	0.0246	0.0309	0.0640	0.1284	0.7644	0.2122	8.0760	0.1369	0.0992	0.6930	0.0006	0.1620	0.2089	0.0156	2.3177	0.2763	0.4178	0.1142	0.0123	0.0452	1.8067	9.5574	0.0290	0.0871	0.3681	0.0293	0.4939	0.9278	0.1192	0.8730
Zv_1	Zv_4_1.5_1	Zoarces viviparus	Zv_4_1.5h	Injection of 13C PHE + sampling	2021-04-10	2021-04-11	1.5	4	23.0	55.0		f/2	0.152079000	0.148588	0.0164	0.0600	0.1220	0.1396	0.0432	0.0310	4.7083	0.2847	1.1796	6.4953	0.1152	1.6798	0.0373	0.0308	0.4487	0.0682	0.0803	0.0316	0.0595	0.6558	0.1324	14.5872	0.0793	0.0219	0.7860	0.0020	0.2154	0.5451	0.0778	20.7425	0.6353	0.4538	0.0922	0.0319	3.1740	1.1557	14.5741	0.0282	0.1732	0.3694	0.0826	0.1034	4.3384	0.1220	1.1269
Zv_2	Zv_4_3_1	Zoarces viviparus	Zv_4_3h	Injection of 13C PHE + sampling	2021-04-10	2021-04-11	3.0	4	23.0	38.0		f/2	0.113467000	0.157282	0.0171	0.0301	0.1112	0.0994	0.0303	0.0255	8.4564	0.0177	0.9317	8.9885	0.0799	1.3164	0.0734	0.0259	0.1400	0.0495	0.0277	0.0245	0.0702	0.2810	0.0734	9.6797	0.0415	0.0281	0.4888	0.0043	0.3279	1.0107	0.0245	10.8787	0.3302	0.3734	0.0438	0.0315	0.0580	0.9889	14.1683	0.0191	0.1993	0.9666	0.0702	0.0477	5.4549	0.0903	0.6894
Zv_3	Zv_10_1.5_1	Zoarces viviparus	Zv_10_1.5h	Injection of 13C PHE + sampling	2021-04-10	2021-04-13	1.5	10	21.5	46.0	0.720	f/2-3	0.274427000	0.172307	0.0151	0.0386	0.0875	0.1147	0.0275	0.0238	5.8558	0.1239	0.9260	6.3884	0.0702	1.1610	0.0566	0.0303	0.1105	0.0450	0.0289	0.0307	0.0479	0.8256	0.1022	10.1474	0.0660	0.0069	0.2977	0.0015	0.1353	1.1211	0.0482	16.2010	0.5187	0.3196	0.0397	0.0239	0.8967	0.5458	11.8555	0.0283	0.2804	0.2882	0.0856	0.1081	4.0478	0.1032	1.4535
Zv_4	Zv_10_3_1	Zoarces viviparus	Zv_10_3h	Injection of 13C PHE + sampling	2021-04-10	2021-04-13	3.0	10	21.0	20.5	0.370	f/2	0.209441000		0.0110	0.0199	0.0887	0.2036	0.0828	0.0400	1.5647	0.2621	1.9489	9.9593	0.0996	1.7843	0.0311	0.0267	0.1868	0.0466	0.0267	0.0350	0.1194	0.7883	0.1579	13.6793	0.0999	0.0372	0.7771	0.0024	0.1092	0.6027	0.0280	15.2151	0.4857	0.5117	0.1064	0.0292	3.0693	0.9544	14.8542	0.0484	0.3286	0.4900	0.0996	0.1354	6.3584	0.1405	1.0646
Zv_5	Zv_13_1.5_1	Zoarces viviparus	Zv_13_1.5h	Injection of 13C PHE + sampling	2021-04-10	2021-04-14	1.5	13	23.0	45.0	0.950	f/2-3	0.175760000	0.115874	0.0080	0.0452	0.1146	0.0911	0.0386	0.0233	3.5140	0.2877	1.0556	9.4782	0.0941	1.5968	0.0721	0.0208	0.1469	0.0496	0.0471	0.0933	0.0501	0.4180	0.0758	14.3260	0.0438	0.0367	1.1980	0.0031	0.1662	0.4395	0.0256	19.6236	0.4721	0.4986	0.0964	0.0209	3.6402	0.8768	14.7551	0.0281	0.2977	0.5710	0.0733	0.0860	4.5230	0.1238	0.9379
Zv_6	Zv_13_3_1	Zoarces viviparus	Zv_13_3h	Injection of 13C PHE + sampling	2021-04-10	2021-04-14	3.0	13	23.5	63.0	1.000	f/2-3	0.151509000	0.235791	0.0214	0.0250	0.1124	0.1169	0.0313	0.0189	4.0781	0.0855	1.3341	6.8514	0.0686	2.3930	0.0581	0.0272	0.0860	0.0415	0.0120	0.1016	0.0661	1.0248	0.1014	8.1707	0.0608	0.0289	0.5330	0.0034	0.2293	1.3332	0.0183	20.2671	0.3548	0.4986	0.1481	0.0180	0.5756	5.5581	11.7780	0.0343	0.3415	0.5964	0.0888	0.0377	6.3865	0.1330	1.3495
Zv_7	Zv_16_1.5_1	Zoarces viviparus	Zv_16_1.5h	Injection of 13C PHE + sampling	2021-04-10	2021-04-15	1.5	16	22.0	46.0	1.310	f/2-3	0.383413000	0.118487	0.0171	0.0452	0.1136	0.1146	0.0236	0.0109	2.9506	0.2262	1.3455	10.7792	0.1225	1.4785	0.0551	0.0413	0.1120	0.0527	0.0273	0.0708	0.0599	0.4299	0.1008	16.3319	0.0538	0.0653	0.8475	0.0021	0.0988	1.0278	0.0378	20.9043	0.6182	0.4659	0.0916	0.0210	3.3272	0.6122	14.5457	0.0364	0.3099	0.6371	0.0748	0.0326	2.4114	0.0887	1.2437
Zv_8	Zv_16_3_1	Zoarces viviparus	Zv_16_3h	Injection of 13C PHE + sampling	2021-04-10	2021-04-15	3.0	16	22.5	46.0	0.500	f/2	0.290999000	0.154132	0.0317	0.0402	0.0771	0.1756	0.0386	0.0137	13.0779	0.0884	0.6792	10.6495	0.0985	1.1961	0.0575	0.0378	0.0762	0.0453	0.0186	0.0696	0.0547	0.4536	0.1469	15.4971	0.0850	0.0276	1.0422	0.0009	0.1827	0.8958	0.0398	16.9165	0.2706	0.3411	0.0684	0.0264	0.8745	0.7171	15.2771	0.0629	0.0764	0.3178	0.0633	0.0263	2.3277	0.0658	1.1580
Zv_9	Zv_22_1.5_1	Zoarces viviparus	Zv_22_1.5h	Injection of 13C PHE + sampling	2021-04-10	2021-04-17	1.5	22	19.0	30.0	0.528	m/2	0.454396000	0.160545	0.0102	0.0342	0.0850	0.1499	0.0296	0.0223	9.5973	0.1095	0.9594	8.1662	0.0627	2.0595	0.0575	0.0387	0.0624	0.0638	0.0124	0.0823	0.0793	0.4936	0.1200	9.2150	0.0643	0.0451	0.9145	0.0026	0.1968	1.3792	0.0612	13.6353	0.9650	0.4659	0.1215	0.0142	0.8428	3.7148	14.1417	0.0603	0.0510	0.6344	0.0748	0.1142	3.2016	0.0937	1.5997
Zv_10	Zv_22_3_1	Zoarces viviparus	Zv_22_3h	Injection of 13C PHE + sampling	2021-04-10	2021-04-17	3.0	22	24.0	42.0	0.391	f/2	0.322831000	0.092038
Zv_11	Zv_4_1.5_2	Zoarces viviparus	Zv_4_1.5h	Injection of 13C PHE + sampling	2021-04-18	2021-04-19	1.5	4	24.0	70.0	1.490	f/3	0.170136000	0.191730	0.0161	0.0362	0.1449	0.0979	0.0378	0.0209	7.2359	0.2391	1.4711	8.2731	0.0773	2.3379	0.0475	0.0256	0.6479	0.0804	0.0546	0.0598	0.0623	0.7295	0.0798	11.0264	0.0499	0.0106	0.2733	0.0022	0.1697	1.3867	0.0486	18.9189	0.5228	0.4334	0.0896	0.0161	1.5083	2.2762	13.0162	0.0379	0.1168	0.3759	0.1167	0.0873	5.2516	0.1222	1.6427
Zv_12	Zv_4_3_2	Zoarces viviparus	Zv_4_3h	Injection of 13C PHE + sampling	2021-04-18	2021-04-19	3.0	4	24.0	61.0	2.270	f/2-3	0.154794000	0.223393
Zv_13	Zv_10_1.5_2	Zoarces viviparus	Zv_10_1.5h	Injection of 13C PHE + sampling	2021-04-18	2021-04-21	1.5	10	21.0	32.0	0.513	m/2	0.151878000	0.105902	0.0189	0.0461	0.1289	0.2713	0.1085	0.0439	4.2391	0.4591	2.1787	8.3701	0.0901	1.7504	0.1310	0.0460	0.4533	0.1059	0.0713	0.0572	0.0755	1.0969	0.2323	10.2656	0.1518	0.0523	0.7402	0.0037	0.2188	1.0128	0.0602	20.2108	0.3964	0.6721	0.0913	0.0255	3.5991	2.9429	13.3722	0.0222	0.5088	0.6508	0.1551	0.0819	6.2598	0.1257	1.0883
Zv_14	Zv_10_3_2	Zoarces viviparus	Zv_10_3h	Injection of 13C PHE + sampling	2021-04-18	2021-04-21	3.0	10	26.0	66.0	0.845	f/5	0.166335000	0.291967	0.0192	0.0351	0.0370	0.1463	0.0863	0.0316	12.2085	0.0737	1.2999	15.1460	0.0690	1.8130	0.0854	0.0460	0.1535	0.0742	0.0406	0.0336	0.1037	0.4559	0.1502	7.1684	0.0958	0.0523	0.4595	0.0025	0.2593	1.0528	0.0379	7.5630	0.3727	0.5616	0.0842	0.0209	0.5051	4.2479	11.9962	0.0293	0.1886	0.4011	0.0857	0.0442	6.0699	0.1213	1.0193
Zv_15	Zv_13_1.5_2	Zoarces viviparus	Zv_13_1.5h	Injection of 13C PHE + sampling	2021-04-18	2021-04-22	1.5	13	22.0	37.0	0.470	f/2	0.468212000	0.250092	0.0459	0.0375	0.0538	0.1672	0.0395	0.0262	6.6612	0.0842	1.4722	11.3183	0.1018	1.9372	0.1669	0.0460	0.1006	0.0682	0.0691	0.0574	0.0835	0.4559	0.1678	13.0323	0.0892	0.0339	0.6435	0.0016	0.1185	1.0025	0.1861	11.2561	0.5679	0.6591	0.1004	0.0267	2.0582	2.0013	17.6603	0.0203	0.1782	1.1316	0.0912	0.0530	6.0699	0.0690	1.3458
Zv_16	Zv_13_3_2	Zoarces viviparus	Zv_13_3h	Injection of 13C PHE + sampling	2021-04-18	2021-04-22	3.0	13	22.0	38.0	0.650	f/2	0.158445000	0.318010	0.0188	0.0273	0.0671	0.0978	0.0553	0.0286	3.4095	0.2194	1.6085	8.1362	0.0692	1.4701	0.1046	0.0209	0.1424	0.0538	0.0421	0.0630	0.0835	0.5405	0.0835	10.5250	0.0485	0.0175	0.7059	0.0039	0.1161	0.8702	0.1486	17.0703	0.3493	0.4052	0.0803	0.0202	2.0582	2.4366	13.3423	0.0262	0.3235	0.5815	0.1245	0.0791	6.0699	0.1035	1.3931
Zv_17	Zv_16_1.5_2	Zoarces viviparus	Zv_16_1.5h	Injection of 13C PHE + sampling	2021-04-18	2021-04-23	1.5	16	23.0	46.0	0.561	m/2	0.344989000	0.090982	0.0157	0.0462	0.0714	0.1394	0.0358	0.0286	1.4134	0.4397	1.3106	9.9507	0.0856	1.5070	0.1532	0.0264	0.1339	0.0368	0.0445	0.0559	0.0702	0.7066	0.1202	15.2197	0.0681	0.0249	0.8854	0.0040	0.0991	0.3206	0.0502	16.6292	0.5787	0.5063	0.1215	0.0153	6.2009	1.3953	14.6218	0.0330	0.1109	0.5379	0.1727	0.0791	3.2468	0.1001	1.1811
Zv_18	Zv_16_3_2	Zoarces viviparus	Zv_16_3h	Injection of 13C PHE + sampling	2021-04-18	2021-04-23	3.0	16	18.5	29.0	0.505	f/2	0.431702000	0.166632	0.0199	0.0529	0.0844	0.0760	0.0950	0.0238	13.7019	0.0729	0.7334	5.4547	0.0925	1.6985	0.0591	0.0302	0.2548	0.0457	0.0490	0.0340	0.0897	0.7066	0.0761	11.0372	0.0388	0.0148	0.6799	0.0014	0.0955	1.0862	0.0502	7.7136	0.7291	0.5899	0.0963	0.0249	0.5402	1.6181	14.9412	0.0333	0.0230	0.3537	0.1299	0.2800	5.0961	0.1108	1.6720
Zv_19	Zv_22_1.5_2	Zoarces viviparus	Zv_22_1.5h	Injection of 13C PHE + sampling	2021-04-18	2021-04-25	1.5	22	18.5	25.0	0.424	f/5	0.334203000	0.111491	0.0147	0.0511	0.1073	0.1404	0.0502	0.0205	5.3712	0.2555	1.0346	9.0834	0.0771	2.1382	0.0797	0.0436	0.2548	0.0507	0.0563	0.0592	0.0735	0.7066	0.1288	12.6956	0.0759	0.0516	1.1196	0.0047	0.1999	0.5325	0.0324	8.6004	0.5692	0.5161	0.2037	0.0275	3.6085	1.8184	14.0435	0.0766	0.1118	0.4442	0.2156	0.1864	4.0044	0.1435	1.0003
Zv_20	Zv_22_3_2	Zoarces viviparus	Zv_22_3h	Injection of 13C PHE + sampling	2021-04-18	2021-04-25	3.0	22	19.0	31.0	0.514	f/3	0.212592000		0.0165	0.0504	0.0841	0.1191	0.0622	0.0248	2.7234	0.1887	0.8693	9.0834	0.0578	2.0169	0.0687	0.0436	0.1486	0.0345	0.0427	0.0632	0.0638	0.4949	0.0987	19.1754	0.0566	0.0516	1.5991	0.0041	0.1072	0.4045	0.0277	16.7908	0.7316	0.4607	0.0992	0.0177	4.6189	0.4540	15.3610	0.0364	0.0490	0.5823	0.2361	0.1028	3.2421	0.0781	0.6408
Zv_21	Zv_4_1.5_3	Zoarces viviparus	Zv_4_1.5h	Injection of 13C PHE + sampling	2021-04-26	2021-04-27	1.5	4	25.0	66.0	1.320	f/3	0.138901000	0.188121	0.0142	0.0299	0.0567	0.0818	0.0402	0.0349	6.8071	0.2773	1.7808	11.3312	0.0700	2.1256	0.1994	0.0255	0.3612	0.0592	0.0335	0.0732	0.0766	0.7112	0.0734	9.4624	0.0410	0.0131	0.2227	0.0005	0.0517	0.7327	0.0657	15.6913	0.7920	0.3768	0.1229	0.0127	3.0105	2.6777	13.1942	0.0216	0.1055	0.7780	0.1170	0.2684	5.3212	0.1573	1.7909
Zv_22	Zv_4_3_3	Zoarces viviparus	Zv_4_3h	Injection of 13C PHE + sampling	2021-04-26	2021-04-27	3.0	4	24.0	47.0	0.710	m/2	0.156957000		0.0198	0.0337	0.0375	0.0674	0.0402	0.0375	16.9281	0.0434	1.1116	7.9795	0.0596	1.7560	0.0947	0.0306	0.2461	0.0654	0.0483	0.0302	0.0339	0.4008	0.0761	10.1108	0.0353	0.0328	0.6216	0.0014	0.1794	1.1445	0.0851	4.2905	0.3973	0.5069	0.2372	0.0219	0.3186	2.6777	13.7207	0.0444	0.0817	0.7780	0.1245	0.1322	9.1133	0.1682	1.1010
Zv_23	Zv_10_1.5_3	Zoarces viviparus	Zv_10_1.5h	Injection of 13C PHE + sampling	2021-04-26	2021-04-29	1.5	10	25.0	62.0	1.480	f/2	0.216628000	0.176198	0.0356	0.0390	0.0548	0.2345	0.0929	0.0317	1.6905	0.3082	1.5380	8.8300	0.0544	2.2742	0.1234	0.0333	0.1477	0.0646	0.0372	0.0512	0.0934	1.0655	0.2062	11.5335	0.1284	0.0805	0.6893	0.0017	0.1265	0.5836	0.0249	20.9333	0.3762	0.4499	0.1035	0.0219	3.5840	2.4709	15.3290	0.0254	0.2994	0.6222	0.0842	0.0843	6.6604	0.1409	1.2499
Zv_24	Zv_10_3_3	Zoarces viviparus	Zv_10_3h	Injection of 13C PHE + sampling	2021-04-26	2021-04-29	3.0	10	24.0	67.0	1.080	f/2	0.162471000	0.299343	0.0081	0.0484	0.0695	0.0787	0.0356	0.0249	13.2020	0.1700	0.6285	6.7446	0.0418	1.5638	0.0538	0.0286	0.2021	0.0246	0.0384	0.0134	0.0081	0.3713	0.0871	10.5436	0.0398	0.0455	0.9707	0.0010	0.2392	0.2800	0.0190	9.8950	0.5388	0.5145	0.3850	0.0299	1.4492	1.1627	14.3400	0.0470	0.2028	0.2825	0.1298	0.0683	6.6604	0.1780	0.6336
Zv_25	Zv_13_1.5_3	Zoarces viviparus	Zv_13_1.5h	Injection of 13C PHE + sampling	2021-04-26	2021-04-30	1.5	13	23.0	52.0	0.935	f/2	0.239219000	0.210659	0.0236	0.0279	0.0888	0.1067	0.0518	0.0317	12.0920	0.0042	1.1948	10.2341	0.1044	1.2967	0.0976	0.0377	0.1235	0.0425	0.0468	0.0661	0.0391	0.8147	0.1051	17.4702	0.0542	0.0247	1.2198	0.0008	0.1795	0.7585	0.0604	12.6499	0.5926	0.4461	0.0619	0.0346	1.1908	0.5561	15.2422	0.0819	0.1290	0.5630	0.0555	0.0236	4.2662	0.1528	1.0775
Zv_26	Zv_13_3_3	Zoarces viviparus	Zv_13_3h	Injection of 13C PHE + sampling	2021-04-26	2021-04-30	3.0	13	23.0	54.0	0.773	f/2	0.244469000		0.0269	0.0392	0.1368	0.2264	0.0494	0.0307	2.5944	0.0609	0.9536	7.7367	0.1039	1.8714	0.0617	0.0312	0.1502	0.0488	0.0513	0.0527	0.0757	0.8692	0.1895	13.4698	0.1191	0.0185	0.6645	0.0030	0.1677	0.9606	0.0477	22.3469	0.6065	0.5010	0.0708	0.0172	1.6211	1.4903	13.8136	0.0176	0.3958	0.7385	0.1053	0.1498	3.3482	0.0863	1.4215
Zv_27	Zv_16_1.5_3	Zoarces viviparus	Zv_16_1.5h	Injection of 13C PHE + sampling	2021-04-26	2021-05-01	1.5	16	23.0	54.0	0.840	f/2	0.238646000	0.085886
Zv_28	Zv_16_3_3	Zoarces viviparus	Zv_16_3h	Injection of 13C PHE + sampling	2021-04-26	2021-05-01	3.0	16	19.5	21.0	0.350	m/2	0.581988000	0.108609
Zv_29	Zv_22_1.5_3	Zoarces viviparus	Zv_22_1.5h	Injection of 13C PHE + sampling	2021-04-26	2021-05-03	1.5	22	21.0	35.0	0.540	f/2	0.208678000	0.207751	0.0668	0.0556	0.1243	0.1659	0.0622	0.0406	5.5881	0.1673	1.1814	11.3127	0.0713	2.9982	0.0580	0.0419	0.1665	0.0522	0.0497	0.0558	0.0766	0.5456	0.1450	6.5744	0.0780	0.0228	0.3492	0.0030	0.2990	0.7589	0.0691	14.6286	0.9126	0.6749	0.1683	0.0059	3.0345	6.0102	15.2538	0.0832	0.0814	0.1546	0.1612	0.1407	4.4637	0.2081	2.8702
Zv_30	Zv_22_3_3	Zoarces viviparus	Zv_22_3h	Injection of 13C PHE + sampling	2021-04-26	2021-05-03	3.0	22	21.0	28.0	0.390	m/2	0.338497000	0.153989