Citation:

PANGAEA.962952

Name: DIC concentration and isotopic composition in sedimentay 13C-labeling incubation of sediment core HE483/25-2
Published: 2024-01-08
License: https://creativecommons.org/licenses/by/4.0/
Keywords: amino acids; DIC; lipidomics; Priming effect; RNA-SIP; Secondary production; stable isotope probing

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Abstract:

DIC measurement: an aliquot of the slurry was filtered with a 0.2-µm filter to remove cells and other particles. After filtration, 1 mL of samples were acidified with 100 μL 45% phosphoric acid overnight in an Exetainer® vial pre-purged with CO2-free air before analysis. All samples were measured with a Delta Ray Isotope Ratio Infrared Spectrometer (IRIS) with URI Connect and autosampler (Thermo Fisher Scientific, Germany) with an analytical error of ±1‰. All isotopic values are reported in the delta notation as δ13C relative to the Vienna PeeDee Belemnite (VPDB) standard. Deviations of triplicate isotopic measurement of sample DIC were between ± 1‰ and ± 1,000‰ (for DIC with label uptake of >10,000‰). DIC concentration was calculated from the released amount of CO2 by calibration with sodium hydrogen carbonate solution.

Keyword(s):

amino acids; DIC; lipidomics; Priming effect; RNA-SIP; Secondary production; stable isotope probing

Supplement to:

Zhu, Qing-Zeng; Yin, Xiuran; Taubner, Heidi; Wendt, Jenny; Friedrich, Michael W; Elvert, Marcus; Hinrichs, Kai-Uwe; Middelburg, Jack J (in prep.): Secondary production and priming reshape the organic matter composition in marine sediments.

Project(s):

Center for Marine Environmental Sciences (MARUM)

The Ocean Floor - Earth's Uncharted Interface (ClusterOceanFloor)

Funding:

German Research Foundation (DFG), grant/award no. 390741603 : EXC 2077: The Ocean Floor – Earth's Uncharted Interface

Coverage:

Latitude: 54.049840 * Longitude: 7.965630

Date/Time Start: 2017-04-26T08:49:00 * Date/Time End: 2017-04-26T08:49:00

Minimum DEPTH, sediment/rock: 0.325 m * Maximum DEPTH, sediment/rock: 2.325 m

Event(s):

HE483/25-2 * Latitude: 54.049840 * Longitude: 7.965630 * Date/Time: 2017-04-26T08:49:00 * Elevation: -26.4 m * Location: North Sea * Campaign: * Basis: * Method/Device: MultiCorer (MUC)

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	DEPTH, sediment/rock	Depth sed	m	Zhu, Qing-Zeng		Geocode
2	Depth, top/min	Depth top	m	Zhu, Qing-Zeng
3	Depth, bottom/max	Depth bot	m	Zhu, Qing-Zeng
4	Sample ID	Sample ID		Zhu, Qing-Zeng
5	Substrate type	Substrate		Zhu, Qing-Zeng
6	Time in days	Time	days	Zhu, Qing-Zeng
7	δ13C, dissolved inorganic carbon	δ13C DIC	‰ PDB	Zhu, Qing-Zeng	Delta Ray Isotope Ratio Infrared Spectrometer (IRIS) with URI Connect and autosampler (Thermo Fisher Scientific, Germany)
8	Carbon, inorganic, dissolved	DIC	µmol/l	Zhu, Qing-Zeng	Delta Ray Isotope Ratio Infrared Spectrometer (IRIS) with URI Connect and autosampler (Thermo Fisher Scientific, Germany)
9	Volume	Vol	ml	Zhu, Qing-Zeng		Incubation volumn

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

560 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Depth sed [m]	2 Depth top [m]	3 Depth bot [m]	4 Sample ID	5 Substrate	6 Time [days]	7 δ13C DIC [‰ PDB]	8 DIC [µmol/l]	9 Vol [ml]
0.325	0.2	0.45	sl0	no substrate	0	-12	700	46
0.325	0.2	0.45	sl0	no substrate	0	-12	700	46
0.325	0.2	0.45	sl1	no substrate	46	-12	800	46
0.325	0.2	0.45	sl5	no substrate	46	-12	760	46
0.325	0.2	0.45	sp3	no substrate	180	-12	850	46
0.325	0.2	0.45	sp3	no substrate	180	-12	1260	46
0.325	0.2	0.45	sl2	no substrate	400	-12	1150	46
0.325	0.2	0.45	sl3	no substrate	400	-14	1220	46
0.325	0.2	0.45	sl3	no substrate	400	-11	1130	46
0.325	0.2	0.45	sp5	no substrate	400	-15	1240	46
0.325	0.2	0.45	sp4	no substrate	400	-11	1020	46
0.325	0.2	0.45	sp4	no substrate	400	-13	1150	46
2.325	2.2	2.45	bl0	no substrate	0	9	4170	46
2.325	2.2	2.45	bl0	no substrate	0	9	4170	46
2.325	2.2	2.45	bl1	no substrate	46	10	4370	46
2.325	2.2	2.45	bl5	no substrate	46	8	4500	46
2.325	2.2	2.45	bp3	no substrate	180	6	5430	46
2.325	2.2	2.45	bp3	no substrate	180	7	3870	46
2.325	2.2	2.45	bl2	no substrate	400	8	5170	46
2.325	2.2	2.45	bl3	no substrate	400	6	5040	46
2.325	2.2	2.45	bl3	no substrate	400	7	5040	46
2.325	2.2	2.45	bp1	no substrate	400	4	4110	46
2.325	2.2	2.45	bp5	no substrate	400	5	4480	46
2.325	2.2	2.45	bp4	no substrate	400	5	4110	46
2.325	2.2	2.45	bp4	no substrate	400	7	3650	46
0.325	0.2	0.45	sl0	d13C-algal lipids	0	-12	700	46
0.325	0.2	0.45	sl0	d13C-algal lipids	0	-12	700	46
0.325	0.2	0.45	sl8	d13C-algal lipids	8	1939	740	46
0.325	0.2	0.45	sl10	d13C-algal lipids	46	14119	1090	46
0.325	0.2	0.45	sl11	d13C-algal lipids	46	14034	870	46
0.325	0.2	0.45	sl13	d13C-algal lipids	180	11157	1170	46
0.325	0.2	0.45	sl14	d13C-algal lipids	180	14442	1020	46
0.325	0.2	0.45	sl15	d13C-algal lipids	180	13421	1460	46
0.325	0.2	0.45	sl15	d13C-algal lipids	400	11513	2110	46
0.325	0.2	0.45	sl16	d13C-algal lipids	400	10876	2020	46
0.325	0.2	0.45	sl17	d13C-algal lipids	400	11822	2000	46
2.325	2.2	2.45	bl0	d13C-algal lipids	0	9	4170	46
2.325	2.2	2.45	bl0	d13C-algal lipids	0	9	4170	46
2.325	2.2	2.45	bl7	d13C-algal lipids	8	388	4300	46
2.325	2.2	2.45	bl8	d13C-algal lipids	8	374	4350	46
2.325	2.2	2.45	bl10	d13C-algal lipids	46	2295	5780	46
2.325	2.2	2.45	bl12	d13C-algal lipids	46	2438	4630	46
2.325	2.2	2.45	bl13	d13C-algal lipids	180	3065	4960	46
2.325	2.2	2.45	bl14	d13C-algal lipids	180	3008	6020	46
2.325	2.2	2.45	bl14	d13C-algal lipids	180	3330	5300	46
2.325	2.2	2.45	bl15	d13C-algal lipids	180	2941	5720	46
2.325	2.2	2.45	bl15	d13C-algal lipids	400	3542	6670	46
2.325	2.2	2.45	bl16	d13C-algal lipids	400	3475	6540	46
2.325	2.2	2.45	bl16	d13C-algal lipids	400	3326	6540	46
0.325	0.2	0.45	sl0	d13C-algal proteins	0	-12	700	46
0.325	0.2	0.45	sl0	d13C-algal proteins	0	-12	700	46
0.325	0.2	0.45	sp13	d13C-algal proteins	13	1976	800	46
0.325	0.2	0.45	sp16	d13C-algal proteins	33	3763	780	46
0.325	0.2	0.45	sp17	d13C-algal proteins	33	2508	740	46
0.325	0.2	0.45	sp19	d13C-algal proteins	180	3857	1300	46
0.325	0.2	0.45	sp20	d13C-algal proteins	180	4495	1130	46
0.325	0.2	0.45	sp21	d13C-algal proteins	400	4192	1150	46
0.325	0.2	0.45	sp22	d13C-algal proteins	400	3890	1330	46
0.325	0.2	0.45	sp23	d13C-algal proteins	400	4346	1240	46
2.325	2.2	2.45	bl0	d13C-algal proteins	0	9	4170	46
2.325	2.2	2.45	bl0	d13C-algal proteins	0	9	4170	46
2.325	2.2	2.45	bp13	d13C-algal proteins	13	813	4000	46
2.325	2.2	2.45	bp14	d13C-algal proteins	13	782	4630	46
2.325	2.2	2.45	bp16	d13C-algal proteins	25	898	4170	46
2.325	2.2	2.45	bp17	d13C-algal proteins	25	745	3630	46
2.325	2.2	2.45	bp19	d13C-algal proteins	180	1119	4240	46
2.325	2.2	2.45	bp20	d13C-algal proteins	180	1097	4760	46
2.325	2.2	2.45	bp21	d13C-algal proteins	400	1087	5170	46
2.325	2.2	2.45	bp22	d13C-algal proteins	400	1207	4590	46
2.325	2.2	2.45	bp23	d13C-algal proteins	400	1231	4630	46