Citation:

PANGAEA.962949

Name: Amino acid concentration and isotopic composition in sedimentary 13C-labeling incubation of sediment core HE483/25-2
Published: 2024-01-08
License: https://creativecommons.org/licenses/by/4.0/
Keywords: amino acids; DIC; lipidomics; Priming effect; RNA-SIP; Secondary production; stable isotope probing

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Abstract:

Amino acids derivatization and analysis: Sediment slurries were centrifuged to separate the sediments from the medium and the sediments were freeze-dried and homogenized. 50 mg of samples spiked with the internal reference standard norleucine were decalcified and acid hydrolyzed (6M, 110℃) for 15 h. The hydrolysate was centrifuged to remove the sediments and subsequently defatted with hexane/ DCM mixture (V/V, 2:1) three times. The purification and derivatization of amino acids were performed by following the protocols described by Takano et al.53 and Chikaraishi et al.54, respectively. In brief, the hydrolysates were evaporated under N2 gas to dryness and purified using Dowex 50WX8 200 400 mesh cation exchange resin to eliminate the matrix effects. The purified amino acids were then isopropylated with a mixture of isopropanol and acetyl chloride (4/1, v/v) at 100 ℃ for 2 h. The solution was then evaporated to dryness and followed 3 times with DCM addition and evaporation to remove any remaining reagent. The AA isopropyl esters were then acylated using a mixture of pivaloyl chloride and DCM (1/1, v/v) at 100 ℃ for 2 h to obtain pivaloyl-isopropyl ester (Pv/AA/iPr). The Pv/AA/iPr solution was then evaporated to dryness and followed 3 times DCM addition and evaporation to remove any remaining reagent. Liquid-liquid extraction was performed by using MiliQ water and a hexane/DCM mixture (2/1, v/v). The Pv/AA/iPr were stored frozen (-20℃) and dissolved in ethyl acetate before analysis.

Keyword(s):

amino acids; DIC; lipidomics; Priming effect; RNA-SIP; Secondary production; stable isotope probing

Supplement to:

Zhu, Qing-Zeng; Yin, Xiuran; Taubner, Heidi; Wendt, Jenny; Friedrich, Michael W; Elvert, Marcus; Hinrichs, Kai-Uwe; Middelburg, Jack J (in prep.): Secondary production and priming reshape the organic matter composition in marine sediments.

Project(s):

Center for Marine Environmental Sciences (MARUM)

The Ocean Floor - Earth's Uncharted Interface (ClusterOceanFloor)

Funding:

German Research Foundation (DFG), grant/award no. 390741603 : EXC 2077: The Ocean Floor – Earth's Uncharted Interface

Coverage:

Latitude: 54.049840 * Longitude: 7.965630

Date/Time Start: 2017-04-26T08:49:00 * Date/Time End: 2017-04-26T08:49:00

Minimum DEPTH, sediment/rock: 0.325 m * Maximum DEPTH, sediment/rock: 2.325 m

Event(s):

HE483/25-2 * Latitude: 54.049840 * Longitude: 7.965630 * Date/Time: 2017-04-26T08:49:00 * Elevation: -26.4 m * Location: North Sea * Campaign: * Basis: * Method/Device: MultiCorer (MUC)

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	DEPTH, sediment/rock	Depth sed	m	Zhu, Qing-Zeng		Geocode
2	Depth, top/min	Depth top	m	Zhu, Qing-Zeng
3	Depth, bottom/max	Depth bot	m	Zhu, Qing-Zeng
4	Sample ID	Sample ID		Zhu, Qing-Zeng
5	Substrate type	Substrate		Zhu, Qing-Zeng
6	Time in days	Time	days	Zhu, Qing-Zeng
7	Sample mass	Samp m	g	Zhu, Qing-Zeng
8	Sample type	Samp type		Zhu, Qing-Zeng		Amino acid type
9	Amino acid, δ13C	AA δ13C	‰ PDB	Zhu, Qing-Zeng	see description in data abstract
10	Amino acid, carbon	AA-C	µg/g	Zhu, Qing-Zeng	see description in data abstract

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

3393 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Depth sed [m]	2 Depth top [m]	3 Depth bot [m]	4 Sample ID	5 Substrate	6 Time [days]	7 Samp m [g]	8 Samp type	9 AA δ13C [‰ PDB]	10 AA-C [µg/g]
0.325	0.2	0.45	sp25	d13C-algal proteins	0	5.01	2-AAA	-3	9.6
0.325	0.2	0.45	sp13	d13C-algal proteins	33	4.83	2-AAA		15.2
0.325	0.2	0.45	sp16	d13C-algal proteins	13	4.77	2-AAA		19.5
0.325	0.2	0.45	sp19	d13C-algal proteins	180	4.57	2-AAA	8	12.6
0.325	0.2	0.45	sp21	d13C-algal proteins	400	4.90	2-AAA	-3	21.9
0.325	0.2	0.45	sp25	d13C-algal proteins	0	5.01	GABA		6.3
0.325	0.2	0.45	sp13	d13C-algal proteins	33	4.83	GABA		6.4
0.325	0.2	0.45	sp16	d13C-algal proteins	13	4.77	GABA		13.0
0.325	0.2	0.45	sp19	d13C-algal proteins	180	4.57	GABA		10.9
0.325	0.2	0.45	sp21	d13C-algal proteins	400	4.90	GABA		26.3
0.325	0.2	0.45	sp25	d13C-algal proteins	0	5.01	alanine	467	212.8
0.325	0.2	0.45	sp13	d13C-algal proteins	33	4.83	alanine	396	153.1
0.325	0.2	0.45	sp16	d13C-algal proteins	13	4.77	alanine	298	243.6
0.325	0.2	0.45	sp19	d13C-algal proteins	180	4.57	alanine	397	316.9
0.325	0.2	0.45	sp21	d13C-algal proteins	400	4.90	alanine	382	141.0
0.325	0.2	0.45	sp25	d13C-algal proteins	0	5.01	aspartic acid	134	460.5
0.325	0.2	0.45	sp13	d13C-algal proteins	33	4.83	aspartic acid	125	453.1
0.325	0.2	0.45	sp16	d13C-algal proteins	13	4.77	aspartic acid	92	523.4
0.325	0.2	0.45	sp19	d13C-algal proteins	180	4.57	aspartic acid	117	597.3
0.325	0.2	0.45	sp21	d13C-algal proteins	400	4.90	aspartic acid	113	605.8
0.325	0.2	0.45	sp25	d13C-algal proteins	0	5.01	beta-alanine	-18	24.4
0.325	0.2	0.45	sp13	d13C-algal proteins	33	4.83	beta-alanine		10.2
0.325	0.2	0.45	sp16	d13C-algal proteins	13	4.77	beta-alanine		21.2
0.325	0.2	0.45	sp19	d13C-algal proteins	180	4.57	beta-alanine	-23	29.5
0.325	0.2	0.45	sp21	d13C-algal proteins	400	4.90	beta-alanine	-19	30.7
0.325	0.2	0.45	sp25	d13C-algal proteins	0	5.01	glutamic acid	470	178.7
0.325	0.2	0.45	sp13	d13C-algal proteins	33	4.83	glutamic acid	432	284.2
0.325	0.2	0.45	sp16	d13C-algal proteins	13	4.77	glutamic acid	338	338.4
0.325	0.2	0.45	sp19	d13C-algal proteins	180	4.57	glutamic acid	397	235.5
0.325	0.2	0.45	sp21	d13C-algal proteins	400	4.90	glutamic acid	384	407.1
0.325	0.2	0.45	sp25	d13C-algal proteins	0	5.01	glycine	113	388.1
0.325	0.2	0.45	sp13	d13C-algal proteins	33	4.83	glycine	100	276.1
0.325	0.2	0.45	sp16	d13C-algal proteins	13	4.77	glycine	71	400.7
0.325	0.2	0.45	sp19	d13C-algal proteins	180	4.57	glycine	98	534.4
0.325	0.2	0.45	sp21	d13C-algal proteins	400	4.90	glycine	93	385.1
0.325	0.2	0.45	sp25	d13C-algal proteins	0	5.01	hydroxyproline	-24	23.2
0.325	0.2	0.45	sp13	d13C-algal proteins	33	4.83	hydroxyproline	-28	56.2
0.325	0.2	0.45	sp16	d13C-algal proteins	13	4.77	hydroxyproline	-28	65.5
0.325	0.2	0.45	sp19	d13C-algal proteins	180	4.57	hydroxyproline	-26	38.1
0.325	0.2	0.45	sp21	d13C-algal proteins	400	4.90	hydroxyproline	-23	39.4
0.325	0.2	0.45	sp25	d13C-algal proteins	0	5.01	isoleucine	1260	56.8
0.325	0.2	0.45	sp13	d13C-algal proteins	33	4.83	isoleucine	1130	60.1
0.325	0.2	0.45	sp16	d13C-algal proteins	13	4.77	isoleucine	684	67.9
0.325	0.2	0.45	sp19	d13C-algal proteins	180	4.57	isoleucine	1113	82.4
0.325	0.2	0.45	sp21	d13C-algal proteins	400	4.90	isoleucine	1038	77.5
0.325	0.2	0.45	sp25	d13C-algal proteins	0	5.01	leucine	1443	171.0
0.325	0.2	0.45	sp13	d13C-algal proteins	33	4.83	leucine	1205	228.4
0.325	0.2	0.45	sp16	d13C-algal proteins	13	4.77	leucine	857	245.0
0.325	0.2	0.45	sp19	d13C-algal proteins	180	4.57	leucine	1279	221.4
0.325	0.2	0.45	sp21	d13C-algal proteins	400	4.90	leucine	1081	207.9
0.325	0.2	0.45	sp25	d13C-algal proteins	0	5.01	lysine	-27	7.5
0.325	0.2	0.45	sp13	d13C-algal proteins	33	4.83	lysine	-23	24.6
0.325	0.2	0.45	sp16	d13C-algal proteins	13	4.77	lysine	-25	33.1
0.325	0.2	0.45	sp19	d13C-algal proteins	180	4.57	lysine	-18	11.4
0.325	0.2	0.45	sp21	d13C-algal proteins	400	4.90	lysine	-23	17.9
0.325	0.2	0.45	sp25	d13C-algal proteins	0	5.01	methionine	712	10.2
0.325	0.2	0.45	sp13	d13C-algal proteins	33	4.83	methionine	688	9.8
0.325	0.2	0.45	sp16	d13C-algal proteins	13	4.77	methionine	447	12.7
0.325	0.2	0.45	sp19	d13C-algal proteins	180	4.57	methionine	257	4.9
0.325	0.2	0.45	sp21	d13C-algal proteins	400	4.90	methionine	496	16.7
0.325	0.2	0.45	sp25	d13C-algal proteins	0	5.01	norleucine	-26	464.7
0.325	0.2	0.45	sp13	d13C-algal proteins	33	4.83	norleucine	-30	476.3
0.325	0.2	0.45	sp16	d13C-algal proteins	13	4.77	norleucine	-27	526.1
0.325	0.2	0.45	sp19	d13C-algal proteins	180	4.57	norleucine	-24	501.9
0.325	0.2	0.45	sp21	d13C-algal proteins	400	4.90	norleucine	-26	516.1
0.325	0.2	0.45	sp25	d13C-algal proteins	0	5.01	phenylalanine	1609	125.3
0.325	0.2	0.45	sp13	d13C-algal proteins	33	4.83	phenylalanine	1413	117.9
0.325	0.2	0.45	sp16	d13C-algal proteins	13	4.77	phenylalanine	1090	138.6
0.325	0.2	0.45	sp19	d13C-algal proteins	180	4.57	phenylalanine	1418	164.0
0.325	0.2	0.45	sp21	d13C-algal proteins	400	4.90	phenylalanine	1357	167.6
0.325	0.2	0.45	sp25	d13C-algal proteins	0	5.01	proline	267	115.1
0.325	0.2	0.45	sp13	d13C-algal proteins	33	4.83	proline	228	106.8
0.325	0.2	0.45	sp16	d13C-algal proteins	13	4.77	proline	184	111.7
0.325	0.2	0.45	sp19	d13C-algal proteins	180	4.57	proline	227	187.0
0.325	0.2	0.45	sp21	d13C-algal proteins	400	4.90	proline	238	140.6
0.325	0.2	0.45	sp25	d13C-algal proteins	0	5.01	serine	178	130.8
0.325	0.2	0.45	sp13	d13C-algal proteins	33	4.83	serine	168	228.4
0.325	0.2	0.45	sp16	d13C-algal proteins	13	4.77	serine	126	248.8
0.325	0.2	0.45	sp19	d13C-algal proteins	180	4.57	serine	155	192.6
0.325	0.2	0.45	sp21	d13C-algal proteins	400	4.90	serine	145	187.5
0.325	0.2	0.45	sp25	d13C-algal proteins	0	5.01	threonine	290	89.0
0.325	0.2	0.45	sp13	d13C-algal proteins	33	4.83	threonine	266	193.4
0.325	0.2	0.45	sp16	d13C-algal proteins	13	4.77	threonine	202	206.5
0.325	0.2	0.45	sp19	d13C-algal proteins	180	4.57	threonine	254	129.7
0.325	0.2	0.45	sp21	d13C-algal proteins	400	4.90	threonine	239	130.6
0.325	0.2	0.45	sp25	d13C-algal proteins	0	5.01	tyrosine	1021	85.1
0.325	0.2	0.45	sp13	d13C-algal proteins	33	4.83	tyrosine	759	71.1
0.325	0.2	0.45	sp16	d13C-algal proteins	13	4.77	tyrosine	560	81.4
0.325	0.2	0.45	sp19	d13C-algal proteins	180	4.57	tyrosine	887	112.1
0.325	0.2	0.45	sp21	d13C-algal proteins	400	4.90	tyrosine	814	101.2
0.325	0.2	0.45	sp25	d13C-algal proteins	0	5.01	valine	708	106.9
0.325	0.2	0.45	sp13	d13C-algal proteins	33	4.83	valine	622	100.9
0.325	0.2	0.45	sp16	d13C-algal proteins	13	4.77	valine	463	118.6
0.325	0.2	0.45	sp19	d13C-algal proteins	180	4.57	valine	612	151.7
0.325	0.2	0.45	sp21	d13C-algal proteins	400	4.90	valine	597	116.4
2.325	2.2	2.45	bp25	d13C-algal proteins	0	4.23	2-AAA	-18	14.8
2.325	2.2	2.45	bp13	d13C-algal proteins	13	4.26	2-AAA		24.6
2.325	2.2	2.45	bp16	d13C-algal proteins	25	4.36	2-AAA		28.0
2.325	2.2	2.45	bp19	d13C-algal proteins	180	3.70	2-AAA	-14	14.3
2.325	2.2	2.45	bp21	d13C-algal proteins	400	4.50	2-AAA	-15	31.2
2.325	2.2	2.45	bp25	d13C-algal proteins	0	4.23	GABA	-11	21.0
2.325	2.2	2.45	bp13	d13C-algal proteins	13	4.26	GABA		45.0
2.325	2.2	2.45	bp16	d13C-algal proteins	25	4.36	GABA		33.6
2.325	2.2	2.45	bp19	d13C-algal proteins	180	3.70	GABA	-24	20.0
2.325	2.2	2.45	bp21	d13C-algal proteins	400	4.50	GABA		45.1
2.325	2.2	2.45	bp25	d13C-algal proteins	0	4.23	alanine	366	365.4
2.325	2.2	2.45	bp13	d13C-algal proteins	13	4.26	alanine	315	414.2
2.325	2.2	2.45	bp16	d13C-algal proteins	25	4.36	alanine	291	415.5
2.325	2.2	2.45	bp19	d13C-algal proteins	180	3.70	alanine	324	417.4
2.325	2.2	2.45	bp21	d13C-algal proteins	400	4.50	alanine	203	189.2
2.325	2.2	2.45	bp25	d13C-algal proteins	0	4.23	aspartic acid	101	684.7
2.325	2.2	2.45	bp13	d13C-algal proteins	13	4.26	aspartic acid	95	734.2
2.325	2.2	2.45	bp16	d13C-algal proteins	25	4.36	aspartic acid	85	789.5
2.325	2.2	2.45	bp19	d13C-algal proteins	180	3.70	aspartic acid	90	762.0
2.325	2.2	2.45	bp21	d13C-algal proteins	400	4.50	aspartic acid	50	858.3
2.325	2.2	2.45	bp25	d13C-algal proteins	0	4.23	beta-alanine	-24	40.4
2.325	2.2	2.45	bp13	d13C-algal proteins	13	4.26	beta-alanine		57.7
2.325	2.2	2.45	bp16	d13C-algal proteins	25	4.36	beta-alanine		40.9
2.325	2.2	2.45	bp19	d13C-algal proteins	180	3.70	beta-alanine	-23	48.1
2.325	2.2	2.45	bp21	d13C-algal proteins	400	4.50	beta-alanine	-23	45.0
2.325	2.2	2.45	bp25	d13C-algal proteins	0	4.23	glutamic acid	371	261.1
2.325	2.2	2.45	bp13	d13C-algal proteins	13	4.26	glutamic acid	353	441.4
2.325	2.2	2.45	bp16	d13C-algal proteins	25	4.36	glutamic acid	323	481.5
2.325	2.2	2.45	bp19	d13C-algal proteins	180	3.70	glutamic acid	331	284.7
2.325	2.2	2.45	bp21	d13C-algal proteins	400	4.50	glutamic acid	209	584.0
2.325	2.2	2.45	bp25	d13C-algal proteins	0	4.23	glycine	84	638.0
2.325	2.2	2.45	bp13	d13C-algal proteins	13	4.26	glycine	77	712.3
2.325	2.2	2.45	bp16	d13C-algal proteins	25	4.36	glycine	69	711.4
2.325	2.2	2.45	bp19	d13C-algal proteins	180	3.70	glycine	75	694.8
2.325	2.2	2.45	bp21	d13C-algal proteins	400	4.50	glycine	41	553.0
2.325	2.2	2.45	bp25	d13C-algal proteins	0	4.23	hydroxyproline	-19	45.6
2.325	2.2	2.45	bp13	d13C-algal proteins	13	4.26	hydroxyproline	-24	90.0
2.325	2.2	2.45	bp16	d13C-algal proteins	25	4.36	hydroxyproline	-26	96.7
2.325	2.2	2.45	bp19	d13C-algal proteins	180	3.70	hydroxyproline	-22	53.5
2.325	2.2	2.45	bp21	d13C-algal proteins	400	4.50	hydroxyproline	-24	58.6
2.325	2.2	2.45	bp25	d13C-algal proteins	0	4.23	isoleucine	987	91.1
2.325	2.2	2.45	bp13	d13C-algal proteins	13	4.26	isoleucine	885	95.8
2.325	2.2	2.45	bp16	d13C-algal proteins	25	4.36	isoleucine	761	101.2
2.325	2.2	2.45	bp19	d13C-algal proteins	180	3.70	isoleucine	897	88.5
2.325	2.2	2.45	bp21	d13C-algal proteins	400	4.50	isoleucine	595	99.3
2.325	2.2	2.45	bp25	d13C-algal proteins	0	4.23	leucine	1107	287.7
2.325	2.2	2.45	bp13	d13C-algal proteins	13	4.26	leucine	1011	319.8
2.325	2.2	2.45	bp16	d13C-algal proteins	25	4.36	leucine	914	355.9
2.325	2.2	2.45	bp19	d13C-algal proteins	180	3.70	leucine	993	325.6
2.325	2.2	2.45	bp21	d13C-algal proteins	400	4.50	leucine	647	271.8
2.325	2.2	2.45	bp25	d13C-algal proteins	0	4.23	lysine	-22	12.3
2.325	2.2	2.45	bp13	d13C-algal proteins	13	4.26	lysine	-22	36.3
2.325	2.2	2.45	bp16	d13C-algal proteins	25	4.36	lysine	-25	42.0
2.325	2.2	2.45	bp19	d13C-algal proteins	180	3.70	lysine	-21	15.7
2.325	2.2	2.45	bp21	d13C-algal proteins	400	4.50	lysine	-26	25.5
2.325	2.2	2.45	bp25	d13C-algal proteins	0	4.23	methionine	577	26.9
2.325	2.2	2.45	bp13	d13C-algal proteins	13	4.26	methionine	552	20.4
2.325	2.2	2.45	bp16	d13C-algal proteins	25	4.36	methionine	372	21.0
2.325	2.2	2.45	bp19	d13C-algal proteins	180	3.70	methionine	613	25.5
2.325	2.2	2.45	bp21	d13C-algal proteins	400	4.50	methionine	277	14.0
2.325	2.2	2.45	bp25	d13C-algal proteins	0	4.23	norleucine	-25	455.6
2.325	2.2	2.45	bp13	d13C-algal proteins	13	4.26	norleucine	-26	475.0
2.325	2.2	2.45	bp16	d13C-algal proteins	25	4.36	norleucine	-27	536.6
2.325	2.2	2.45	bp19	d13C-algal proteins	180	3.70	norleucine	-28	503.9
2.325	2.2	2.45	bp21	d13C-algal proteins	400	4.50	norleucine	-27	520.3
2.325	2.2	2.45	bp25	d13C-algal proteins	0	4.23	phenylalanine	1304	180.3
2.325	2.2	2.45	bp13	d13C-algal proteins	13	4.26	phenylalanine	1232	182.6
2.325	2.2	2.45	bp16	d13C-algal proteins	25	4.36	phenylalanine	1116	195.6
2.325	2.2	2.45	bp19	d13C-algal proteins	180	3.70	phenylalanine	1176	214.8
2.325	2.2	2.45	bp21	d13C-algal proteins	400	4.50	phenylalanine	738	228.1
2.325	2.2	2.45	bp25	d13C-algal proteins	0	4.23	proline	209	234.6
2.325	2.2	2.45	bp13	d13C-algal proteins	13	4.26	proline	206	208.0
2.325	2.2	2.45	bp16	d13C-algal proteins	25	4.36	proline	181	220.8
2.325	2.2	2.45	bp19	d13C-algal proteins	180	3.70	proline	193	257.8
2.325	2.2	2.45	bp21	d13C-algal proteins	400	4.50	proline	127	198.0
2.325	2.2	2.45	bp25	d13C-algal proteins	0	4.23	serine	132	223.0
2.325	2.2	2.45	bp13	d13C-algal proteins	13	4.26	serine	131	276.3
2.325	2.2	2.45	bp16	d13C-algal proteins	25	4.36	serine	118	415.9
2.325	2.2	2.45	bp19	d13C-algal proteins	180	3.70	serine	120	258.5
2.325	2.2	2.45	bp21	d13C-algal proteins	400	4.50	serine	67	276.8
2.325	2.2	2.45	bp25	d13C-algal proteins	0	4.23	threonine	229	152.9
2.325	2.2	2.45	bp13	d13C-algal proteins	13	4.26	threonine	204	127.7
2.325	2.2	2.45	bp16	d13C-algal proteins	25	4.36	threonine	195	328.9
2.325	2.2	2.45	bp19	d13C-algal proteins	180	3.70	threonine	206	160.3
2.325	2.2	2.45	bp21	d13C-algal proteins	400	4.50	threonine	121	189.0
2.325	2.2	2.45	bp25	d13C-algal proteins	0	4.23	tyrosine	842	118.9
2.325	2.2	2.45	bp13	d13C-algal proteins	13	4.26	tyrosine	683	99.0
2.325	2.2	2.45	bp16	d13C-algal proteins	25	4.36	tyrosine	608	111.8
2.325	2.2	2.45	bp19	d13C-algal proteins	180	3.70	tyrosine	750	137.8
2.325	2.2	2.45	bp21	d13C-algal proteins	400	4.50	tyrosine	461	130.7
2.325	2.2	2.45	bp25	d13C-algal proteins	0	4.23	valine	559	174.5
2.325	2.2	2.45	bp13	d13C-algal proteins	13	4.26	valine	526	169.1
2.325	2.2	2.45	bp16	d13C-algal proteins	25	4.36	valine	470	180.0
2.325	2.2	2.45	bp19	d13C-algal proteins	180	3.70	valine	502	168.5
2.325	2.2	2.45	bp21	d13C-algal proteins	400	4.50	valine	332	145.0
2.325	2.2	2.45	sl1	d13C-algal lipids	0	5.09	2-AAA	-25	20.4
2.325	2.2	2.45	sl7	d13C-algal lipids	8	5.07	2-AAA	-20	19.4
2.325	2.2	2.45	sl10	d13C-algal lipids	46	4.98	2-AAA	-4	20.4
2.325	2.2	2.45	sl13	d13C-algal lipids	180	4.18	2-AAA	-24	12.4
2.325	2.2	2.45	sl15	d13C-algal lipids	400	4.60	2-AAA	-20	18.7
2.325	2.2	2.45	sl1	d13C-algal lipids	0	5.09	GABA		24.8
2.325	2.2	2.45	sl7	d13C-algal lipids	8	5.07	GABA		23.6
2.325	2.2	2.45	sl10	d13C-algal lipids	46	4.98	GABA	-20	23.7
2.325	2.2	2.45	sl13	d13C-algal lipids	180	4.18	GABA	-26	12.6
2.325	2.2	2.45	sl15	d13C-algal lipids	400	4.60	GABA		15.8
2.325	2.2	2.45	sl1	d13C-algal lipids	0	5.09	alanine	-28	266.4
2.325	2.2	2.45	sl7	d13C-algal lipids	8	5.07	alanine	15	263.9
2.325	2.2	2.45	sl10	d13C-algal lipids	46	4.98	alanine	58	274.3
2.325	2.2	2.45	sl13	d13C-algal lipids	180	4.18	alanine	44	241.1
2.325	2.2	2.45	sl15	d13C-algal lipids	400	4.60	alanine	33	142.5
2.325	2.2	2.45	sl1	d13C-algal lipids	0	5.09	aspartic acid	-27	556.6
2.325	2.2	2.45	sl7	d13C-algal lipids	8	5.07	aspartic acid	0	526.2
2.325	2.2	2.45	sl10	d13C-algal lipids	46	4.98	aspartic acid	33	556.1
2.325	2.2	2.45	sl13	d13C-algal lipids	180	4.18	aspartic acid	25	577.0
2.325	2.2	2.45	sl15	d13C-algal lipids	400	4.60	aspartic acid	18	546.1
2.325	2.2	2.45	sl1	d13C-algal lipids	0	5.09	beta-alanine		36.3
2.325	2.2	2.45	sl7	d13C-algal lipids	8	5.07	beta-alanine		37.6
2.325	2.2	2.45	sl10	d13C-algal lipids	46	4.98	beta-alanine		34.3
2.325	2.2	2.45	sl13	d13C-algal lipids	180	4.18	beta-alanine	-22	30.1
2.325	2.2	2.45	sl15	d13C-algal lipids	400	4.60	beta-alanine	-22	24.0
2.325	2.2	2.45	sl1	d13C-algal lipids	0	5.09	glutamic acid	-27	359.2
2.325	2.2	2.45	sl7	d13C-algal lipids	8	5.07	glutamic acid	51	343.6
2.325	2.2	2.45	sl10	d13C-algal lipids	46	4.98	glutamic acid	116	368.0
2.325	2.2	2.45	sl13	d13C-algal lipids	180	4.18	glutamic acid	85	231.4
2.325	2.2	2.45	sl15	d13C-algal lipids	400	4.60	glutamic acid	57	376.1
2.325	2.2	2.45	sl1	d13C-algal lipids	0	5.09	glycine	-27	463.6
2.325	2.2	2.45	sl7	d13C-algal lipids	8	5.07	glycine	-12	421.2
2.325	2.2	2.45	sl10	d13C-algal lipids	46	4.98	glycine	1	457.4
2.325	2.2	2.45	sl13	d13C-algal lipids	180	4.18	glycine	-3	497.3
2.325	2.2	2.45	sl15	d13C-algal lipids	400	4.60	glycine	-5	355.0
2.325	2.2	2.45	sl1	d13C-algal lipids	0	5.09	hydroxyproline	-28	34.3
2.325	2.2	2.45	sl7	d13C-algal lipids	8	5.07	hydroxyproline	-27	34.3
2.325	2.2	2.45	sl10	d13C-algal lipids	46	4.98	hydroxyproline	-29	28.9
2.325	2.2	2.45	sl13	d13C-algal lipids	180	4.18	hydroxyproline	-27	36.9
2.325	2.2	2.45	sl15	d13C-algal lipids	400	4.60	hydroxyproline	-27	45.5
2.325	2.2	2.45	sl1	d13C-algal lipids	0	5.09	isoleucine	-31	65.5
2.325	2.2	2.45	sl7	d13C-algal lipids	8	5.07	isoleucine	74	64.6
2.325	2.2	2.45	sl10	d13C-algal lipids	46	4.98	isoleucine	198	53.0
2.325	2.2	2.45	sl13	d13C-algal lipids	180	4.18	isoleucine	161	59.6
2.325	2.2	2.45	sl15	d13C-algal lipids	400	4.60	isoleucine	120	69.2
2.325	2.2	2.45	sl1	d13C-algal lipids	0	5.09	leucine	-29	272.9
2.325	2.2	2.45	sl7	d13C-algal lipids	8	5.07	leucine	63	265.7
2.325	2.2	2.45	sl10	d13C-algal lipids	46	4.98	leucine	145	270.9
2.325	2.2	2.45	sl13	d13C-algal lipids	180	4.18	leucine	147	227.6
2.325	2.2	2.45	sl15	d13C-algal lipids	400	4.60	leucine	99	185.4
2.325	2.2	2.45	sl1	d13C-algal lipids	0	5.09	lysine	-26	10.2
2.325	2.2	2.45	sl7	d13C-algal lipids	8	5.07	lysine	-27	10.0
2.325	2.2	2.45	sl10	d13C-algal lipids	46	4.98	lysine		7.8
2.325	2.2	2.45	sl13	d13C-algal lipids	180	4.18	lysine	-24	8.9
2.325	2.2	2.45	sl15	d13C-algal lipids	400	4.60	lysine	-26	17.2
2.325	2.2	2.45	sl1	d13C-algal lipids	0	5.09	methionine	-29	32.5
2.325	2.2	2.45	sl7	d13C-algal lipids	8	5.07	methionine	38	10.0
2.325	2.2	2.45	sl10	d13C-algal lipids	46	4.98	methionine	135	8.9
2.325	2.2	2.45	sl13	d13C-algal lipids	180	4.18	methionine	129	16.9
2.325	2.2	2.45	sl15	d13C-algal lipids	400	4.60	methionine	64	12.2
2.325	2.2	2.45	sl1	d13C-algal lipids	0	5.09	norleucine	-30	518.2
2.325	2.2	2.45	sl7	d13C-algal lipids	8	5.07	norleucine	-30	486.7
2.325	2.2	2.45	sl10	d13C-algal lipids	46	4.98	norleucine	-30	507.9
2.325	2.2	2.45	sl13	d13C-algal lipids	180	4.18	norleucine	-29	508.9
2.325	2.2	2.45	sl15	d13C-algal lipids	400	4.60	norleucine	-28	499.0
2.325	2.2	2.45	sl1	d13C-algal lipids	0	5.09	phenylalanine	-29	145.4
2.325	2.2	2.45	sl7	d13C-algal lipids	8	5.07	phenylalanine	81	142.2
2.325	2.2	2.45	sl10	d13C-algal lipids	46	4.98	phenylalanine	174	147.2
2.325	2.2	2.45	sl13	d13C-algal lipids	180	4.18	phenylalanine	150	152.3
2.325	2.2	2.45	sl15	d13C-algal lipids	400	4.60	phenylalanine	111	145.3
2.325	2.2	2.45	sl1	d13C-algal lipids	0	5.09	proline	-25	147.4
2.325	2.2	2.45	sl7	d13C-algal lipids	8	5.07	proline	25	148.1
2.325	2.2	2.45	sl10	d13C-algal lipids	46	4.98	proline	75	159.1
2.325	2.2	2.45	sl13	d13C-algal lipids	180	4.18	proline	61	159.8
2.325	2.2	2.45	sl15	d13C-algal lipids	400	4.60	proline	42	123.6
2.325	2.2	2.45	sl1	d13C-algal lipids	0	5.09	serine	-28	162.0
2.325	2.2	2.45	sl7	d13C-algal lipids	8	5.07	serine	-7	128.2
2.325	2.2	2.45	sl10	d13C-algal lipids	46	4.98	serine	18	142.9
2.325	2.2	2.45	sl13	d13C-algal lipids	180	4.18	serine	12	175.6
2.325	2.2	2.45	sl15	d13C-algal lipids	400	4.60	serine	3	181.4
2.325	2.2	2.45	sl1	d13C-algal lipids	0	5.09	threonine	-28	88.6
2.325	2.2	2.45	sl7	d13C-algal lipids	8	5.07	threonine	3	76.1
2.325	2.2	2.45	sl10	d13C-algal lipids	46	4.98	threonine	40	83.0
2.325	2.2	2.45	sl13	d13C-algal lipids	180	4.18	threonine	29	112.6
2.325	2.2	2.45	sl15	d13C-algal lipids	400	4.60	threonine	20	123.7
2.325	2.2	2.45	sl1	d13C-algal lipids	0	5.09	tyrosine	-28	87.5
2.325	2.2	2.45	sl7	d13C-algal lipids	8	5.07	tyrosine	37	82.7
2.325	2.2	2.45	sl10	d13C-algal lipids	46	4.98	tyrosine	105	67.6
2.325	2.2	2.45	sl13	d13C-algal lipids	180	4.18	tyrosine	86	108.6
2.325	2.2	2.45	sl15	d13C-algal lipids	400	4.60	tyrosine	69	93.7
2.325	2.2	2.45	sl1	d13C-algal lipids	0	5.09	valine	-31	127.4
2.325	2.2	2.45	sl7	d13C-algal lipids	8	5.07	valine	33	131.8
2.325	2.2	2.45	sl10	d13C-algal lipids	46	4.98	valine	98	112.3
2.325	2.2	2.45	sl13	d13C-algal lipids	180	4.18	valine	75	108.2
2.325	2.2	2.45	sl15	d13C-algal lipids	400	4.60	valine	59	107.0
2.325	2.2	2.45	bl1	d13C-algal lipids	0	4.00	2-AAA	-26	27.7
2.325	2.2	2.45	bl7	d13C-algal lipids	8	4.29	2-AAA	-21	25.0
2.325	2.2	2.45	bl10	d13C-algal lipids	46	4.18	2-AAA	-19	28.0
2.325	2.2	2.45	bl13	d13C-algal lipids	180	4.10	2-AAA	-31	16.5
2.325	2.2	2.45	bl15	d13C-algal lipids	400	4.00	2-AAA	-21	25.7
2.325	2.2	2.45	bl1	d13C-algal lipids	0	4.00	GABA		43.4
2.325	2.2	2.45	bl7	d13C-algal lipids	8	4.29	GABA	-26	31.7
2.325	2.2	2.45	bl10	d13C-algal lipids	46	4.18	GABA	-24	41.9
2.325	2.2	2.45	bl13	d13C-algal lipids	180	4.10	GABA	-18	24.8
2.325	2.2	2.45	bl15	d13C-algal lipids	400	4.00	GABA		45.0
2.325	2.2	2.45	bl1	d13C-algal lipids	0	4.00	alanine	-28	404.8
2.325	2.2	2.45	bl7	d13C-algal lipids	8	4.29	alanine	-9	373.8
2.325	2.2	2.45	bl10	d13C-algal lipids	46	4.18	alanine	19	430.7
2.325	2.2	2.45	bl13	d13C-algal lipids	180	4.10	alanine	28	338.7
2.325	2.2	2.45	bl15	d13C-algal lipids	400	4.00	alanine	24	181.3
2.325	2.2	2.45	bl1	d13C-algal lipids	0	4.00	aspartic acid	-27	800.6
2.325	2.2	2.45	bl7	d13C-algal lipids	8	4.29	aspartic acid	-15	717.8
2.325	2.2	2.45	bl10	d13C-algal lipids	46	4.18	aspartic acid	6	848.2
2.325	2.2	2.45	bl13	d13C-algal lipids	180	4.10	aspartic acid	10	729.9
2.325	2.2	2.45	bl15	d13C-algal lipids	400	4.00	aspartic acid	5	814.3
2.325	2.2	2.45	bl1	d13C-algal lipids	0	4.00	beta-alanine	-28	54.3
2.325	2.2	2.45	bl7	d13C-algal lipids	8	4.29	beta-alanine	-21	45.7
2.325	2.2	2.45	bl10	d13C-algal lipids	46	4.18	beta-alanine		58.3
2.325	2.2	2.45	bl13	d13C-algal lipids	180	4.10	beta-alanine	-26	47.3
2.325	2.2	2.45	bl15	d13C-algal lipids	400	4.00	beta-alanine	-24	42.2
2.325	2.2	2.45	bl1	d13C-algal lipids	0	4.00	glutamic acid	-27	519.3
2.325	2.2	2.45	bl7	d13C-algal lipids	8	4.29	glutamic acid	8	463.9
2.325	2.2	2.45	bl10	d13C-algal lipids	46	4.18	glutamic acid	56	543.3
2.325	2.2	2.45	bl13	d13C-algal lipids	180	4.10	glutamic acid	53	277.9
2.325	2.2	2.45	bl15	d13C-algal lipids	400	4.00	glutamic acid	34	540.7
2.325	2.2	2.45	bl1	d13C-algal lipids	0	4.00	glycine	-27	677.2
2.325	2.2	2.45	bl7	d13C-algal lipids	8	4.29	glycine	-20	621.8
2.325	2.2	2.45	bl10	d13C-algal lipids	46	4.18	glycine	-11	767.2
2.325	2.2	2.45	bl13	d13C-algal lipids	180	4.10	glycine	-8	647.6
2.325	2.2	2.45	bl15	d13C-algal lipids	400	4.00	glycine	-9	544.5
2.325	2.2	2.45	bl1	d13C-algal lipids	0	4.00	hydroxyproline	-28	62.5
2.325	2.2	2.45	bl7	d13C-algal lipids	8	4.29	hydroxyproline	-28	60.4
2.325	2.2	2.45	bl10	d13C-algal lipids	46	4.18	hydroxyproline	-29	57.0
2.325	2.2	2.45	bl13	d13C-algal lipids	180	4.10	hydroxyproline	-28	108.1
2.325	2.2	2.45	bl15	d13C-algal lipids	400	4.00	hydroxyproline	-27	46.8
2.325	2.2	2.45	bl1	d13C-algal lipids	0	4.00	isoleucine	-30	78.5
2.325	2.2	2.45	bl7	d13C-algal lipids	8	4.29	isoleucine	20	75.6
2.325	2.2	2.45	bl10	d13C-algal lipids	46	4.18	isoleucine	92	87.1
2.325	2.2	2.45	bl13	d13C-algal lipids	180	4.10	isoleucine	114	72.0
2.325	2.2	2.45	bl15	d13C-algal lipids	400	4.00	isoleucine	91	107.0
2.325	2.2	2.45	bl1	d13C-algal lipids	0	4.00	leucine	-29	369.1
2.325	2.2	2.45	bl7	d13C-algal lipids	8	4.29	leucine	11	308.6
2.325	2.2	2.45	bl10	d13C-algal lipids	46	4.18	leucine	47	384.0
2.325	2.2	2.45	bl13	d13C-algal lipids	180	4.10	leucine	83	265.8
2.325	2.2	2.45	bl15	d13C-algal lipids	400	4.00	leucine	72	257.7
2.325	2.2	2.45	bl1	d13C-algal lipids	0	4.00	lysine		20.5
2.325	2.2	2.45	bl7	d13C-algal lipids	8	4.29	lysine	-28	23.0
2.325	2.2	2.45	bl10	d13C-algal lipids	46	4.18	lysine	-27	21.4
2.325	2.2	2.45	bl13	d13C-algal lipids	180	4.10	lysine	-23	11.7
2.325	2.2	2.45	bl15	d13C-algal lipids	400	4.00	lysine	-22	16.6
2.325	2.2	2.45	bl1	d13C-algal lipids	0	4.00	methionine	-31	11.9
2.325	2.2	2.45	bl7	d13C-algal lipids	8	4.29	methionine	-28	14.6
2.325	2.2	2.45	bl10	d13C-algal lipids	46	4.18	methionine	39	17.1
2.325	2.2	2.45	bl13	d13C-algal lipids	180	4.10	methionine	106	31.6
2.325	2.2	2.45	bl15	d13C-algal lipids	400	4.00	methionine	19	6.4
2.325	2.2	2.45	bl1	d13C-algal lipids	0	4.00	norleucine	-30	528.8
2.325	2.2	2.45	bl7	d13C-algal lipids	8	4.29	norleucine	-31	439.9
2.325	2.2	2.45	bl10	d13C-algal lipids	46	4.18	norleucine	-31	493.2
2.325	2.2	2.45	bl13	d13C-algal lipids	180	4.10	norleucine	-29	507.9
2.325	2.2	2.45	bl15	d13C-algal lipids	400	4.00	norleucine	-28	472.4
2.325	2.2	2.45	bl1	d13C-algal lipids	0	4.00	phenylalanine	-29	217.7
2.325	2.2	2.45	bl7	d13C-algal lipids	8	4.29	phenylalanine	26	186.3
2.325	2.2	2.45	bl10	d13C-algal lipids	46	4.18	phenylalanine	78	200.1
2.325	2.2	2.45	bl13	d13C-algal lipids	180	4.10	phenylalanine	103	195.9
2.325	2.2	2.45	bl15	d13C-algal lipids	400	4.00	phenylalanine	110	203.8
2.325	2.2	2.45	bl1	d13C-algal lipids	0	4.00	proline	-24	214.3
2.325	2.2	2.45	bl7	d13C-algal lipids	8	4.29	proline	-3	181.9
2.325	2.2	2.45	bl10	d13C-algal lipids	46	4.18	proline	24	221.8
2.325	2.2	2.45	bl13	d13C-algal lipids	180	4.10	proline	31	265.1
2.325	2.2	2.45	bl15	d13C-algal lipids	400	4.00	proline	27	183.2
2.325	2.2	2.45	bl1	d13C-algal lipids	0	4.00	serine	-28	238.6
2.325	2.2	2.45	bl7	d13C-algal lipids	8	4.29	serine	-20	247.3
2.325	2.2	2.45	bl10	d13C-algal lipids	46	4.18	serine	-2	237.6
2.325	2.2	2.45	bl13	d13C-algal lipids	180	4.10	serine	3	312.3
2.325	2.2	2.45	bl15	d13C-algal lipids	400	4.00	serine	-3	216.6
2.325	2.2	2.45	bl1	d13C-algal lipids	0	4.00	threonine	-28	131.3
2.325	2.2	2.45	bl7	d13C-algal lipids	8	4.29	threonine	-15	146.1
2.325	2.2	2.45	bl10	d13C-algal lipids	46	4.18	threonine	6	126.7
2.325	2.2	2.45	bl13	d13C-algal lipids	180	4.10	threonine	11	122.0
2.325	2.2	2.45	bl15	d13C-algal lipids	400	4.00	threonine	8	148.2
2.325	2.2	2.45	bl1	d13C-algal lipids	0	4.00	tyrosine	-27	127.3
2.325	2.2	2.45	bl7	d13C-algal lipids	8	4.29	tyrosine	2	106.9
2.325	2.2	2.45	bl10	d13C-algal lipids	46	4.18	tyrosine	50	115.1
2.325	2.2	2.45	bl13	d13C-algal lipids	180	4.10	tyrosine	67	130.9
2.325	2.2	2.45	bl15	d13C-algal lipids	400	4.00	tyrosine	59	113.1
2.325	2.2	2.45	bl1	d13C-algal lipids	0	4.00	valine	-31	165.3
2.325	2.2	2.45	bl7	d13C-algal lipids	8	4.29	valine	-2	157.4
2.325	2.2	2.45	bl10	d13C-algal lipids	46	4.18	valine	40	185.1
2.325	2.2	2.45	bl13	d13C-algal lipids	180	4.10	valine	52	126.8
2.325	2.2	2.45	bl15	d13C-algal lipids	400	4.00	valine	41	149.6