Citation:

PANGAEA.962719

Name: Multi-method diet analysis to investigate lobster predation on the barren-forming sea urchin Centrostephanus rodgersii
Published: 2023-10-06
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Centrostephanus rodgersii; DNA barcoding; isotope analysis; Jasus edwardsii; range extension; spiny lobster; stomach content analysis

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Published: 2023-10-06 • DOI registered: 2023-11-04

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Abstract:

In Tasmania, Australia, the government's response to range-extending, barren-forming longspined sea urchin (Centrostephanus rodgersii) includes rebuilding of southern rock lobster (Jasus edwardsii) stocks to increase predation. But lobster preference for native species and continued barren expansion challenge the control efficacy. Therefore, this study aimed to determine the impact of lobster predation on C. rodgersii in different coastal habitats using a multi-method dietary analysis comprising 1) stomach contents analysis, 2) targeted DNA detection of prey species in faecal samples and 3) stable isotope analysis (d13C and d15N). The dataset contains diet information from 64 southern rock lobster (Jasus edwardsii) from five sites (four habitat types) in eastern Tasmania, Australia. Investigated prey species included: range-extending longspined sea urchins (Centrostephanus rodgersii), Shortspined sea urchin (Heliocidaris erythrogramma), periwinkle (Lunella undulata) and blacklip abalone (Haliotis rubra).

Keyword(s):

Centrostephanus rodgersii; DNA barcoding; isotope analysis; Jasus edwardsii; range extension; spiny lobster; stomach content analysis

Supplement to:

Smith, Jennifer E; Keane, John; Oellermann, Michael; Gardner, Caleb; Mundy, Craig (in review): Lobster predation on barren-forming sea urchins is more prevalent in habitats where small urchins are common: a multi-method diet analysis. Marine and Freshwater Research

Funding:

Fisheries Research and Development Corporation (FRDC), grant/award no. FRDC 2021-020 : Stable isotopes: a rapid method to determine lobster diet and trace lobster origin

Coverage:

Median Latitude: -42.532846 * Median Longitude: 147.873812 * South-bound Latitude: -43.126040 * West-bound Longitude: 147.355296 * North-bound Latitude: -41.252799 * East-bound Longitude: 148.340382

Date/Time Start: 2020-01-29T00:00:00 * Date/Time End: 2020-12-11T00:00:00

Event(s):

CapePaul_2020 * Latitude: -42.843832 * Longitude: 147.923175 * Date/Time: 2020-01-31T00:00:00 * Location: Cape Paul * Method/Device: Lobster trap, Manufacturer unknown, custom built [Pot dimension 80x80x45 cm; Mesh size 3x3 cm; top entrance circle 30 cm]

CrayfishPoint_2020 * Latitude: -42.952653 * Longitude: 147.355296 * Date/Time: 2020-02-06T00:00:00 * Location: Crayfish Point * Method/Device: Lobster trap, Manufacturer unknown, custom built [Pot dimension 80x80x45 cm; Mesh size 3x3 cm; top entrance circle 30 cm]

ElephantRock_2020 * Latitude: -41.252799 * Longitude: 148.340382 * Date/Time: 2020-12-11T00:00:00 * Location: Elephant Rock * Method/Device: Lobster trap, Manufacturer unknown, custom built [Pot dimension 80x80x45 cm; Mesh size 3x3 cm; top entrance circle 30 cm]

Comment:

This project was further funded by the Holsworth Wildlife Research Endowment – Equity Trustees Charitable Foundation & the Ecological Society of Australia

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Event label	Event		Smith, Jennifer E
2	DATE/TIME	Date/Time		Smith, Jennifer E		Geocode
3	LATITUDE	Latitude		Smith, Jennifer E		Geocode
4	LONGITUDE	Longitude		Smith, Jennifer E		Geocode
5	Country	Country		Smith, Jennifer E
6	Location	Location		Smith, Jennifer E
7	Conservation status	Cons status		Smith, Jennifer E
8	Grazing impact	Grazing imp		Smith, Jennifer E		by Centrostephanus rodgersii
9	Species	Species		Smith, Jennifer E
10	Species, unique identification (URI)	Species UID (URI)		Smith, Jennifer E
11	Sample code/label	Sample label		Smith, Jennifer E
12	Sex	Sex		Smith, Jennifer E
13	Carapace, length	Carap l	mm	Smith, Jennifer E
14	Size group description	Size gr desc		Smith, Jennifer E
15	Prey, DNA present in faeces	Prey DNA pres faeces		Smith, Jennifer E	Polymerase chain reaction (PCR)	of Centrostephanus rodgersii
16	Prey, DNA present in faeces	Prey DNA pres faeces		Smith, Jennifer E	Polymerase chain reaction (PCR)	of Heliocidaris erythrogramma
17	Prey, DNA present in faeces	Prey DNA pres faeces		Smith, Jennifer E	Polymerase chain reaction (PCR)	of Haliotis rubra
18	Prey, DNA present in faeces	Prey DNA pres faeces		Smith, Jennifer E	Polymerase chain reaction (PCR)	of Lunella undulata
19	Jasus edwardsii, δ15N	J. edwardsii δ15N	‰ air	Smith, Jennifer E	Carbon and hydrogen and nitrogen and sulfur and oxygen (CHNSO) isotope elemental analyzer, Elementar, vario PYRO cube; coupled with Isotope ratio mass spectrometer (IRMS), Elementar, IsoPrime100
20	Jasus edwardsii, δ13C	J. edwardsii δ13C	‰ PDB	Smith, Jennifer E	Carbon and hydrogen and nitrogen and sulfur and oxygen (CHNSO) isotope elemental analyzer, Elementar, vario PYRO cube; coupled with Isotope ratio mass spectrometer (IRMS), Elementar, IsoPrime100
21	Jasus edwardsii, stomach content	J. edwardsii stomach cont	#	Smith, Jennifer E	Stereo microscope, Leica, MZ12.5; coupled with digital microscope camera, Leica, DFC450	Counts of Centrostephanus rodgersii
22	Jasus edwardsii, stomach content	J. edwardsii stomach cont	#	Smith, Jennifer E	Stereo microscope, Leica, MZ12.5; coupled with digital microscope camera, Leica, DFC450	Counts of Heliocidaris erythrogramma
23	Jasus edwardsii, stomach content	J. edwardsii stomach cont	#	Smith, Jennifer E	Stereo microscope, Leica, MZ12.5; coupled with digital microscope camera, Leica, DFC450	Counts of Haliotis rubra
24	Jasus edwardsii, stomach content	J. edwardsii stomach cont	#	Smith, Jennifer E	Stereo microscope, Leica, MZ12.5; coupled with digital microscope camera, Leica, DFC450	Counts of other prey

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1258 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Event	2 Date/Time	3 Latitude	4 Longitude	5 Country	6 Location	7 Cons status	8 Grazing imp (by Centrostephanus rodgersii)	9 Species	10 Species UID (URI)	11 Sample label	12 Sex	13 Carap l [mm]	14 Size gr desc	15 Prey DNA pres faeces (of Centrostephanus rodgersii,...)	16 Prey DNA pres faeces (of Heliocidaris erythrogramma...)	17 Prey DNA pres faeces (of Haliotis rubra, Polymerase...)	18 Prey DNA pres faeces (of Lunella undulata, Polymera...)	19 J. edwardsii δ15N [‰ air] (Carbon and hydrogen and nitro...)	20 J. edwardsii δ13C [‰ PDB] (Carbon and hydrogen and nitro...)	21 J. edwardsii stomach cont [#] (Counts of Centrostephanus rod...)	22 J. edwardsii stomach cont [#] (Counts of Heliocidaris erythr...)	23 J. edwardsii stomach cont [#] (Counts of Haliotis rubra, Ste...)	24 J. edwardsii stomach cont [#] (Counts of other prey, Stereo ...)
NorthBay_2020	2020-01-29	-42.864523	147.954132	Australia	North Bay	marine reserve	Incipient barren	Jasus edwardsii	marinespecies.org	1	Male	158	Large	Yes	No	No	No	12.466	-14.940	0	0	0	1
NorthBay_2020	2020-01-29	-42.864523	147.954132	Australia	North Bay	marine reserve	Incipient barren	Jasus edwardsii	marinespecies.org	2	Male	181	Large	Yes	No	No	No	12.112	-14.838	0	0	0	3
NorthBay_2020	2020-01-29	-42.864523	147.954132	Australia	North Bay	marine reserve	Incipient barren	Jasus edwardsii	marinespecies.org	3	Male	97	Small	Yes	No	Yes	No	12.330	-15.761	0	0	0	2
NorthBay_2020	2020-01-29	-42.864523	147.954132	Australia	North Bay	marine reserve	Incipient barren	Jasus edwardsii	marinespecies.org	4	Female	101	Small	Yes	No	No	No	12.473	-15.897	0	0	0	2
NorthBay_2020	2020-01-29	-42.864523	147.954132	Australia	North Bay	marine reserve	Incipient barren	Jasus edwardsii	marinespecies.org	5	Female	128	Medium	Yes	No	No	No	12.997	-14.946	0	0	0	1
NorthBay_2020	2020-01-29	-42.864523	147.954132	Australia	North Bay	marine reserve	Incipient barren	Jasus edwardsii	marinespecies.org	6	Male	165	Large	Yes	No	No	No	12.726	-15.623	0	0	0	1
NorthBay_2020	2020-01-29	-42.864523	147.954132	Australia	North Bay	marine reserve	Incipient barren	Jasus edwardsii	marinespecies.org	7	Female	122	Medium	No	Yes	No	No	13.265	-15.922	0	0	0	1
NorthBay_2020	2020-01-29	-42.864523	147.954132	Australia	North Bay	marine reserve	Incipient barren	Jasus edwardsii	marinespecies.org	8	Female	102	Small	Yes	No	No	No	12.898	-15.301	0	0	0	3
NorthBay_2020	2020-01-29	-42.864523	147.954132	Australia	North Bay	marine reserve	Incipient barren	Jasus edwardsii	marinespecies.org	9	Male	103	Small	No	No	Yes	No	12.570	-16.156	0	0	0	1
NorthBay_2020	2020-01-29	-42.864523	147.954132	Australia	North Bay	marine reserve	Incipient barren	Jasus edwardsii	marinespecies.org	10	Female	126	Medium	No	No	No	No	12.672	-15.073	0	0	0	3
NorthBay_2020	2020-01-29	-42.864523	147.954132	Australia	North Bay	marine reserve	Incipient barren	Jasus edwardsii	marinespecies.org	11	Female	106	Small					13.096	-15.965	0	0	0	3
NorthBay_2020	2020-01-29	-42.864523	147.954132	Australia	North Bay	marine reserve	Incipient barren	Jasus edwardsii	marinespecies.org	12	Male	135	Medium	No	No	No	No	12.537	-15.275	0	0	0	1
NorthBay_2020	2020-01-29	-42.864523	147.954132	Australia	North Bay	marine reserve	Incipient barren	Jasus edwardsii	marinespecies.org	13	Male	102	Small	No	No	No	No	11.520	-16.242	0	0	0	1
NorthBay_2020	2020-01-29	-42.864523	147.954132	Australia	North Bay	marine reserve	Incipient barren	Jasus edwardsii	marinespecies.org	14	Male	126	Medium					12.760	-15.897	0	0	0	1
NorthBay_2020	2020-01-29	-42.864523	147.954132	Australia	North Bay	marine reserve	Incipient barren	Jasus edwardsii	marinespecies.org	15	Male	130	Medium	No	No	No	No	12.061	-16.036	0	0	0	2
Fortescue_2020	2020-01-30	-43.126040	147.979859	Australia	Fortescue	fished	Incipient barren	Jasus edwardsii	marinespecies.org	16	Male	105	Small	Yes	No	No	No	12.672	-15.321	0	0	0	3
Fortescue_2020	2020-01-30	-43.126040	147.979859	Australia	Fortescue	fished	Incipient barren	Jasus edwardsii	marinespecies.org	17	Female	95	Small	Yes	No	No	No	12.789	-16.253	0	0	1	5
Fortescue_2020	2020-01-30	-43.126040	147.979859	Australia	Fortescue	fished	Incipient barren	Jasus edwardsii	marinespecies.org	18	Male	128	Medium	Yes	No	Yes	No	12.234	-16.110	0	0	0	1
Fortescue_2020	2020-01-30	-43.126040	147.979859	Australia	Fortescue	fished	Incipient barren	Jasus edwardsii	marinespecies.org	19	Female	124	Medium	Yes	No	No	Yes	13.188	-14.899	0	0	0	1
Fortescue_2020	2020-01-30	-43.126040	147.979859	Australia	Fortescue	fished	Incipient barren	Jasus edwardsii	marinespecies.org	20	Male	90	Small	Yes	No	No	Yes	12.616	-15.265	0	0	0	5
Fortescue_2020	2020-01-30	-43.126040	147.979859	Australia	Fortescue	fished	Incipient barren	Jasus edwardsii	marinespecies.org	21	Male	122	Medium	Yes	No	No	No	12.352	-14.207	0	0	0	4
CrayfishPoint_2020	2020-02-06	-42.952653	147.355296	Australia	Crayfish Point	marine reserve	No Centrostephanus rodgersii	Jasus edwardsii	marinespecies.org	22	Male	145	Large	No	No	No	No	12.249	-17.000	0	0	0	1
CrayfishPoint_2020	2020-02-06	-42.952653	147.355296	Australia	Crayfish Point	marine reserve	No Centrostephanus rodgersii	Jasus edwardsii	marinespecies.org	23	Male	156	Large	No	No	No	No	13.083	-18.548	0	0	0	0
CrayfishPoint_2020	2020-02-06	-42.952653	147.355296	Australia	Crayfish Point	marine reserve	No Centrostephanus rodgersii	Jasus edwardsii	marinespecies.org	24	Female	134	Medium	No	No	No	No	12.998	-17.221	0	0	0	0
CrayfishPoint_2020	2020-02-06	-42.952653	147.355296	Australia	Crayfish Point	marine reserve	No Centrostephanus rodgersii	Jasus edwardsii	marinespecies.org	25	Female	120	Medium	No	No	Yes	No			0	0	0	3
CrayfishPoint_2020	2020-02-06	-42.952653	147.355296	Australia	Crayfish Point	marine reserve	No Centrostephanus rodgersii	Jasus edwardsii	marinespecies.org	26	Female	130	Medium	No	No	No	No	13.693	-17.212	0	0	0	3
CrayfishPoint_2020	2020-02-06	-42.952653	147.355296	Australia	Crayfish Point	marine reserve	No Centrostephanus rodgersii	Jasus edwardsii	marinespecies.org	28	Male	148	Large	No	No	Yes	No	12.940	-17.668	0	0	0	5
CapePaul_2020	2020-01-31	-42.843832	147.923175	Australia	Cape Paul	fished	Incipient barren	Jasus edwardsii	marinespecies.org	31	Male	146	Large	Yes	No	No	No	12.781	-17.961	0	0	0	4
CapePaul_2020	2020-01-31	-42.843832	147.923175	Australia	Cape Paul	fished	Incipient barren	Jasus edwardsii	marinespecies.org	32	Male	145	Large	Yes	No	Yes	No	12.288	-17.367	0	0	0	1
CapePaul_2020	2020-01-31	-42.843832	147.923175	Australia	Cape Paul	fished	Incipient barren	Jasus edwardsii	marinespecies.org	33	Male	102	Small	Yes	No	Yes	No	12.451	-15.498	0	0	0	1
CapePaul_2020	2020-01-31	-42.843832	147.923175	Australia	Cape Paul	fished	Incipient barren	Jasus edwardsii	marinespecies.org	34	Female	126	Medium	Yes	No	Yes	No	13.025	-15.948	0	0	0	1
CapePaul_2020	2020-01-31	-42.843832	147.923175	Australia	Cape Paul	fished	Incipient barren	Jasus edwardsii	marinespecies.org	35	Male	142	Large	Yes	No	No	No	12.714	-15.883	0	0	0	3
CapePaul_2020	2020-01-31	-42.843832	147.923175	Australia	Cape Paul	fished	Incipient barren	Jasus edwardsii	marinespecies.org	36	Female	65	Small	Yes	No	No	No	12.394	-18.075	0	1	0	3
CapePaul_2020	2020-01-31	-42.843832	147.923175	Australia	Cape Paul	fished	Incipient barren	Jasus edwardsii	marinespecies.org	37	Male	130	Medium	Yes	No	No	Yes	12.101	-15.864	0	0	1	3
CapePaul_2020	2020-01-31	-42.843832	147.923175	Australia	Cape Paul	fished	Incipient barren	Jasus edwardsii	marinespecies.org	38	Female	122	Medium	Yes	No	Yes	No	13.251	-15.526	0	0	0	3
CapePaul_2020	2020-01-31	-42.843832	147.923175	Australia	Cape Paul	fished	Incipient barren	Jasus edwardsii	marinespecies.org	39	Female	128	Medium	Yes	No	No	No	12.473	-15.384	0	0	0	3
CapePaul_2020	2020-01-31	-42.843832	147.923175	Australia	Cape Paul	fished	Incipient barren	Jasus edwardsii	marinespecies.org	40	Female	69	Small	Yes	No	No	No	12.563	-16.772	0	0	1	0
CrayfishPoint_2020	2020-02-05	-42.952653	147.355296	Australia	Crayfish Point	marine reserve	No Centrostephanus rodgersii	Jasus edwardsii	marinespecies.org	41	Male	125	Medium	No	No	Yes	No	14.048	-17.315	0	0	0	5
CrayfishPoint_2020	2020-02-05	-42.952653	147.355296	Australia	Crayfish Point	marine reserve	No Centrostephanus rodgersii	Jasus edwardsii	marinespecies.org	42	Male	125	Medium	No	No	Yes	No	14.042	-17.110	0	0	0	0
CrayfishPoint_2020	2020-02-05	-42.952653	147.355296	Australia	Crayfish Point	marine reserve	No Centrostephanus rodgersii	Jasus edwardsii	marinespecies.org	43	Male	79	Small	No	No	Yes	No	13.563	-17.364	0	0	0	1
CrayfishPoint_2020	2020-02-05	-42.952653	147.355296	Australia	Crayfish Point	marine reserve	No Centrostephanus rodgersii	Jasus edwardsii	marinespecies.org	47	Male	129	Medium	No	No	Yes	No	14.491	-16.810	0	0	0	2
CrayfishPoint_2020	2020-02-05	-42.952653	147.355296	Australia	Crayfish Point	marine reserve	No Centrostephanus rodgersii	Jasus edwardsii	marinespecies.org	48	Female	102	Small	No	No	No	No	14.481	-17.062	0	0	0	1
CrayfishPoint_2020	2020-02-05	-42.952653	147.355296	Australia	Crayfish Point	marine reserve	No Centrostephanus rodgersii	Jasus edwardsii	marinespecies.org	59	Male	148	Large	No	No	Yes	No	13.806	-17.538	0	0	0	3
CrayfishPoint_2020	2020-02-05	-42.952653	147.355296	Australia	Crayfish Point	marine reserve	No Centrostephanus rodgersii	Jasus edwardsii	marinespecies.org	60	Male	86	Small	No	No	Yes	No	13.381	-17.828	0	0	0	4
CrayfishPoint_2020	2020-02-05	-42.952653	147.355296	Australia	Crayfish Point	marine reserve	No Centrostephanus rodgersii	Jasus edwardsii	marinespecies.org	61	Female	109	Small	No	Yes	No	No	14.190	-17.520	0	0	0	2
CrayfishPoint_2020	2020-02-05	-42.952653	147.355296	Australia	Crayfish Point	marine reserve	No Centrostephanus rodgersii	Jasus edwardsii	marinespecies.org	62	Male	91	Small	No	No	No	No	13.269	-17.989	0	0	0	3
CrayfishPoint_2020	2020-02-05	-42.952653	147.355296	Australia	Crayfish Point	marine reserve	No Centrostephanus rodgersii	Jasus edwardsii	marinespecies.org	64	Male	145	Large	No	No	No	No	13.757	-17.271	0	0	0	3
CrayfishPoint_2020	2020-02-05	-42.952653	147.355296	Australia	Crayfish Point	marine reserve	No Centrostephanus rodgersii	Jasus edwardsii	marinespecies.org	66	Female	83	Small	No	No	No	No	13.163	-17.888	0	0	0	1
CrayfishPoint_2020	2020-02-05	-42.952653	147.355296	Australia	Crayfish Point	marine reserve	No Centrostephanus rodgersii	Jasus edwardsii	marinespecies.org	67	Male	154	Large	No	No	No	No	13.634	-17.900	0	0	0	2
ElephantRock_2020	2020-12-11	-41.252799	148.340382	Australia	Elephant Rock	marine reserve	Extensive barren	Jasus edwardsii	marinespecies.org	76	Female	122	Medium	No	No	No	No	13.219	-16.046	0	0	0	3
ElephantRock_2020	2020-12-11	-41.252799	148.340382	Australia	Elephant Rock	marine reserve	Extensive barren	Jasus edwardsii	marinespecies.org	77	Female	101	Small	No	No	No	No	12.667	-15.967	0	0	0	3
ElephantRock_2020	2020-12-11	-41.252799	148.340382	Australia	Elephant Rock	marine reserve	Extensive barren	Jasus edwardsii	marinespecies.org	78	Female	122	Medium	Yes	No	No	No	13.132	-15.744	0	0	0	2
ElephantRock_2020	2020-12-11	-41.252799	148.340382	Australia	Elephant Rock	marine reserve	Extensive barren	Jasus edwardsii	marinespecies.org	80	Male	122	Medium	Yes	No	No	No	12.001	-15.660	0	0	1	5
ElephantRock_2020	2020-12-11	-41.252799	148.340382	Australia	Elephant Rock	marine reserve	Extensive barren	Jasus edwardsii	marinespecies.org	81	Male	139	Medium					12.862	-15.750	0	0	0	2
ElephantRock_2020	2020-12-11	-41.252799	148.340382	Australia	Elephant Rock	marine reserve	Extensive barren	Jasus edwardsii	marinespecies.org	89	Male	201	Large					12.890	-15.214	0	0	0	3
ElephantRock_2020	2020-12-11	-41.252799	148.340382	Australia	Elephant Rock	marine reserve	Extensive barren	Jasus edwardsii	marinespecies.org	90	Male	144	Large	No	No	No	Yes	12.045	-15.540	0	0	0	7
ElephantRock_2020	2020-12-11	-41.252799	148.340382	Australia	Elephant Rock	marine reserve	Extensive barren	Jasus edwardsii	marinespecies.org	91	Male	202	Large	No	No	No	No	12.895	-16.006	1	0	0	0
ElephantRock_2020	2020-12-11	-41.252799	148.340382	Australia	Elephant Rock	marine reserve	Extensive barren	Jasus edwardsii	marinespecies.org	93	Female	144	Large					12.408	-15.897	0	0	0	4
ElephantRock_2020	2020-12-11	-41.252799	148.340382	Australia	Elephant Rock	marine reserve	Extensive barren	Jasus edwardsii	marinespecies.org	94	Male	115	Medium	No	No	No	No	11.580	-16.592	0	0	0	2
ElephantRock_2020	2020-12-11	-41.252799	148.340382	Australia	Elephant Rock	marine reserve	Extensive barren	Jasus edwardsii	marinespecies.org	95	Female	145	Large	Yes	No	No	No	12.845	-15.654	0	0	0	2
ElephantRock_2020	2020-12-11	-41.252799	148.340382	Australia	Elephant Rock	marine reserve	Extensive barren	Jasus edwardsii	marinespecies.org	96	Female	140	Large	No	No	No	No	12.492	-15.578	0	0	0	2
ElephantRock_2020	2020-12-11	-41.252799	148.340382	Australia	Elephant Rock	marine reserve	Extensive barren	Jasus edwardsii	marinespecies.org	97	Female	138	Medium	Yes	No	No	No	12.682	-15.825	0	0	0	1
ElephantRock_2020	2020-12-11	-41.252799	148.340382	Australia	Elephant Rock	marine reserve	Extensive barren	Jasus edwardsii	marinespecies.org	98	Male	93	Small	No	No	No	No	12.337	-16.189	0	0	1	2
ElephantRock_2020	2020-12-11	-41.252799	148.340382	Australia	Elephant Rock	marine reserve	Extensive barren	Jasus edwardsii	marinespecies.org	99	Female	85	Small	No	No	No	Yes	11.953	-17.045	0	0	1	3