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Fernández-Carrera, Ana; Montoya, Joseph P; Subramaniam, Ajit (2023): Biological nitrogen fixation and stable isotopes of carbon in seston during Meteor cruise M130 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.962553

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Abstract:
Biological nitrogen fixation is a key process balancing the loss of combined nitrogen in the marine nitrogen cycle. Its relevance in upwelling systems is not fully resolved. This dataset contains rates of nitrogen fixation through the euphotic layer in two size fractions measured following Montoya et al (1996) technique. It also contains the stable isotopes of carbon in seston expressed in delta notation (δ13C, ‰, VPDB). We sampled in the region of the Guinea Dome and Equatorial Atlantic Ocean along 23°W during Meteor cruise M130 in August-September 2016. Water samples were collected by niskin bottles attached to a rosette equipped with CTD sensors. Incubations were done in on-deck incubators refrigerated by running surface water continuously and simulating the light intensity of each depth by neutral density filters or meshes.
Keyword(s):
biological nitrogen fixation; diazotrophs; Equatorial Atlantic; Guinea Dome
Supplement to:
Fernández-Carrera, Ana; Kiko, Rainer; Hauss, Helena; Hamilton, Douglas S; Achterberg, Eric Pieter; Montoya, Joseph P; Dengler, Marcus; Brandt, Peter; Subramaniam, Ajit (2023): Nitrogen fixation rates in the Guinea Dome and the equatorial upwelling regions in the Atlantic Ocean. Biogeochemistry, https://doi.org/10.1007/s10533-023-01089-w
Related to:
Dengler, Marcus; Krahmann, Gerd (2019): Physical oceanography (CTD) during METEOR cruise M130 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.904367
References:
Montoya, Joseph P; Voss, Maren; Kähler, Paul; Capone, D G (1996): A Simple, High-Precision, High-Sensitivity Tracer Assay for N2 Fixation. Applied and Environmental Microbiology, 62(3), 986-993, https://doi.org/10.1128/aem.62.3.986-993.1996
Funding:
Gordon and Betty Moore Foundation, grant/award no. GBMF4886: Nitrogen Fixation in the Equatorial Atlantic Upwelling System
Xunta de Galicia, grant/award no. POS-A/2013/142: Xunta de Galicia 2013 postdoctoral fellowship
Coverage:
Median Latitude: 6.407904 * Median Longitude: -22.381609 * South-bound Latitude: -5.000000 * West-bound Longitude: -24.213830 * North-bound Latitude: 17.702830 * East-bound Longitude: -18.997500
Date/Time Start: 2016-08-30T19:20:00 * Date/Time End: 2016-09-19T01:51:00
Minimum Elevation: -5860.0 m * Maximum Elevation: -2042.3 m
Event(s):
M130_941-1  * Latitude: 17.702833 * Longitude: -24.213833 * Date/Time: 2016-08-30T19:20:00 * Elevation: -3598.5 m * Campaign: M130 * Basis: Meteor (1986) * Method/Device: CTD/Rosette (CTD-RO)
M130_949-1  * Latitude: 15.000333 * Longitude: -22.999667 * Date/Time: 2016-09-02T10:19:00 * Elevation: -2712.3 m * Campaign: M130 * Basis: Meteor (1986) * Method/Device: CTD/Rosette (CTD-RO)
M130_952-1  * Latitude: 14.499667 * Longitude: -22.999833 * Date/Time: 2016-09-02T15:55:00 * Elevation: -4091.4 m * Campaign: M130 * Basis: Meteor (1986) * Method/Device: CTD/Rosette (CTD-RO)
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
Event labelEventSubramaniam, Ajit
GearGearSubramaniam, Ajit
AbbreviationAbbrevSubramaniam, Ajit
DATE/TIMEDate/TimeSubramaniam, AjitGeocode
LATITUDELatitudeSubramaniam, AjitGeocode
LONGITUDELongitudeSubramaniam, AjitGeocode
ELEVATIONElevationm a.s.l.Subramaniam, AjitGeocode
LocationLocationSubramaniam, Ajit
DEPTH, waterDepth watermSubramaniam, AjitCTD, Sea-Bird, SBE 11 plusGeocode
10 Temperature, waterTemp°CSubramaniam, AjitCTD, Sea-Bird, SBE 11 plusdata from Dengler and Krahmann (2019)
11 SalinitySalSubramaniam, AjitCTD, Sea-Bird, SBE 11 pluswater, PSU (data from Dengler and Krahmann 2019)
12 δ13C, sestonδ13C sest‰ PDBSubramaniam, AjitContinuous flow isotope ratio mass spectrometer (CF-IRMS), Micromass, Isoprime; coupled with Elemental analyser, Carlo Erba, NA 2500VPDB
13 Trichodesmium, carbon in sestonTrichodesmium C sest%Subramaniam, AjitCalculated according to Fry (2006)Percent contribution of Trichodesmium to C in seston derived by two-end-member mixing model. End members δ13C Tricho = -12.9‰, δ13C Phytoplankton = -22‰
14 NitrogenN%Subramaniam, AjitContinuous flow isotope ratio mass spectrometer (CF-IRMS), Micromass, Isoprime; coupled with Elemental analyser, Carlo Erba, NA 2500atom%N at the beginning of incubation, natural abundance (time 0)
15 Sample volumeSamp volmlSubramaniam, AjitV incubated
16 Nitrogen, 15N labeled15N2mlSubramaniam, Ajitadded: Cambridge Isotopes98 atom%, Cambridge Isotopes Lot. #I-19168A
17 Incubation durationInc durhSubramaniam, Ajit
18 Nitrogen, particulate, size fraction <10 µmPN <10 µmµmol/lSubramaniam, AjitContinuous flow isotope ratio mass spectrometer (CF-IRMS), Micromass, Isoprime; coupled with Elemental analyser, Carlo Erba, NA 2500Small T24, avg: Average of Particulate Nitrogen in the <10µm size fraction at the end of incubation (time 24)
19 Nitrogen, particulate, size fraction <10 µm, standard deviationPN <10 µm std dev±Subramaniam, AjitContinuous flow isotope ratio mass spectrometer (CF-IRMS), Micromass, Isoprime; coupled with Elemental analyser, Carlo Erba, NA 2500Small T24 SD: Standard Deviation of Particulate Nitrogen in the <10µm size fraction at the end of incubation (time 24)
20 Nitrogen, particulate, size fraction > 10 µmPN >10 µmµmol/lSubramaniam, AjitContinuous flow isotope ratio mass spectrometer (CF-IRMS), Micromass, Isoprime; coupled with Elemental analyser, Carlo Erba, NA 2500Large T24, avg: Average of Particulate Nitrogen in the >10µm size fraction at the end of incubation (time 24)
21 Nitrogen, particulate, size fraction > 10 µm, standard deviationPN >10 µm std dev±Subramaniam, AjitContinuous flow isotope ratio mass spectrometer (CF-IRMS), Micromass, Isoprime; coupled with Elemental analyser, Carlo Erba, NA 2500Large T24 SD: Standard Deviation of Particulate Nitrogen in the >10µm size fraction at the end of incubation (time 24)
22 Nitrogen fixation rate, size fraction < 10 µmN2 fix <10 µmnmol/l/daySubramaniam, AjitCalculated according to Montoya et al. (1996)BNF small, avg: Average of Biological Nitrogen Fixation (BNF) of the <10µm size fraction. Detection limit 0.05 nmol N L-1 d-1
23 Nitrogen fixation rate, size fraction < 10 µm, standard deviationN2 fix <10 µm std dev±Subramaniam, AjitCalculated according to Montoya et al. (1996)BNF small SD: Standard Deviation of Biological Nitrogen Fixation (BNF) of the <10µm size fraction. Detection limit 0.05 nmol N L-1 d-1
24 Nitrogen fixation rate, size fraction > 10 µmN2 fix >10 µmnmol/l/daySubramaniam, AjitCalculated according to Montoya et al. (1996)BNF large, avg: Average of Biological Nitrogen Fixation (BNF) of the >10µm size fraction. Detection limit 0.05 nmol N L-1 d-1
25 Nitrogen fixation rate, size fraction > 10 µm, standard deviationN2 fix >10 µm std dev±Subramaniam, AjitCalculated according to Montoya et al. (1996)BNF large SD: Standard Deviation of Biological Nitrogen Fixation (BNF) of the >10µm size fraction. Detection limit 0.05 nmol N L-1 d-1
26 Nitrogen fixation rate, totalN2 fix totalnmol/l/daySubramaniam, AjitCalculated according to Montoya et al. (1996)BNF total, avg: Standard Deviation of Biological Nitrogen Fixation (BNF) of the sum of both size fractions. Detection limit 0.05 nmol N L-1 d-1
27 Nitrogen fixation rate, total, standard deviationN2 fix total std dev±Subramaniam, AjitCalculated according to Montoya et al. (1996)BNF total SD: Average of Biological Nitrogen Fixation (BNF) of the sum of both size fractions. Detection limit 0.05 nmol N L-1 d-1
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1101 data points

Data

Download dataset as tab-delimited text — use the following character encoding:


Event

Gear

Abbrev

Date/Time

Latitude

Longitude

Elevation [m a.s.l.]

Location

Depth water [m]
10 
Temp [°C]
11 
Sal
12 
δ13C sest [‰ PDB]
13 
Trichodesmium C sest [%]
14 
N [%]
15 
Samp vol [ml]
16 
15N2 [ml]
17 
Inc dur [h]
18 
PN <10 µm [µmol/l]
19 
PN <10 µm std dev [±]
20 
PN >10 µm [µmol/l]
21 
PN >10 µm std dev [±]
22 
N2 fix <10 µm [nmol/l/day]
23 
N2 fix <10 µm std dev [±]
24 
N2 fix >10 µm [nmol/l/day]
25 
N2 fix >10 µm std dev [±]
26 
N2 fix total [nmol/l/day]
27 
N2 fix total std dev [±]
M130_941-1 CTD/RosetteCTD-RO2016-08-30T19:2017.70283-24.21383-3598Eastern Tropical Atlantic Ocean8.98.926.6-23.20.00.366245433.024.10.300.040.480.050.000.000.480.05
M130_949-1 CTD/RosetteCTD-RO2016-09-02T10:1915.00033-22.99967-2712Eastern Tropical Atlantic Ocean9.127.836.3-22.00.50.366345433.024.30.370.030.110.740.110.321.060.11
M130_952-1 CTD/RosetteCTD-RO2016-09-02T15:5514.49967-22.99983-4091Eastern Tropical Atlantic Ocean9.727.236.3-21.29.10.365045433.024.00.430.0511.903.340.000.0011.903.34
M130_955-1 CTD/RosetteCTD-RO2016-09-02T21:0914.00017-23.00000-4323Eastern Tropical Atlantic Ocean9.328.236.3-21.37.90.364945433.024.00.540.150.210.038.992.4315.317.5224.309.94
M130_957-1 CTD/RosetteCTD-RO2016-09-03T01:5913.50033-23.00017-4539Eastern Tropical Atlantic Ocean9.828.136.3-20.813.00.365045433.024.00.560.130.150.0316.891.5113.812.2230.703.73
M130_959-1 CTD/RosetteCTD-RO2016-09-03T07:0113.00317-22.99967-4742Eastern Tropical Atlantic Ocean12.128.236.0-21.64.80.366245433.024.00.450.061.280.150.000.001.280.15
M130_962-1 CTD/RosetteCTD-RO2016-09-03T13:0112.50067-22.99967-4921Eastern Tropical Atlantic Ocean9.327.536.1-21.19.80.365745433.024.00.370.031.040.060.000.001.040.06
M130_966-1 CTD/RosetteCTD-RO2016-09-03T18:3111.99700-22.99683-5044Eastern Tropical Atlantic Ocean9.528.335.9-22.80.00.363945433.024.00.500.021.270.160.000.001.270.16
M130_969-1 CTD/RosetteCTD-RO2016-09-03T23:5811.46450-22.98883-5514Eastern Tropical Atlantic Ocean9.528.335.9-21.81.70.366745433.023.00.570.120.220.890.220.280.041.170.26
M130_972-1 CTD/RosetteCTD-RO2016-09-04T05:0411.00017-23.00183-5155Eastern Tropical Atlantic Ocean9.128.235.9-21.37.50.366045433.024.00.460.053.480.990.000.003.480.99
M130_974-1 CTD/RosetteCTD-RO2016-09-04T09:5610.49883-22.99900-5192Eastern Tropical Atlantic Ocean8.828.035.7-20.516.20.365145433.024.00.480.080.250.025.782.4815.997.1621.779.63
M130_976-1 CTD/RosetteCTD-RO2016-09-04T14:3810.00333-22.99917-5009Eastern Tropical Atlantic Ocean9.628.135.5-21.64.50.364945433.024.10.530.040.180.046.350.5112.414.3418.764.85
M130_981-1 CTD/RosetteCTD-RO2016-09-04T23:189.51083-23.00767-4637Eastern Tropical Atlantic Ocean10.028.035.6-20.318.60.365345433.024.00.520.080.220.066.260.5816.206.7922.467.36
M130_983-1 CTD/RosetteCTD-RO2016-09-05T04:109.00017-22.99967-4818Eastern Tropical Atlantic Ocean9.528.235.4-22.40.00.364245433.024.00.440.055.490.960.000.005.490.96
M130_989-1 CTD/RosetteCTD-RO2016-09-05T14:568.00083-23.00017-4417Eastern Tropical Atlantic Ocean8.828.135.2-21.82.10.365145433.024.00.310.031.320.400.000.001.320.40
M130_994-1 CTD/RosetteCTD-RO2016-09-06T05:357.99250-20.97467-2822Eastern Tropical Atlantic Ocean73.217.935.8-26.50.00.364845433.024.00.150.000.240.080.000.000.240.08
M130_994-1CTD/RosetteCTD-RO2016-09-06T05:357.99250-20.97467-2822Eastern Tropical Atlantic Ocean27.127.435.7-22.60.00.365245433.024.00.380.030.121.980.353.195.170.35
M130_994-1CTD/RosetteCTD-RO2016-09-06T05:357.99250-20.97467-2822Eastern Tropical Atlantic Ocean9.127.735.5-21.90.70.364845433.024.00.350.070.120.002.510.896.211.658.712.54
M130_998-1 CTD/RosetteCTD-RO2016-09-06T18:197.99950-18.99750-4570Eastern Tropical Atlantic Ocean11.727.735.1-21.46.90.365445433.024.00.450.030.150.023.331.162.790.756.121.91
M130_1002-1 CTD/RosetteCTD-RO2016-09-07T12:129.99983-19.99900-4478Eastern Tropical Atlantic Ocean34.117.335.7-27.20.00.364945433.024.00.300.050.140.010.000.140.01
M130_1002-1CTD/RosetteCTD-RO2016-09-07T12:129.99983-19.99900-4478Eastern Tropical Atlantic Ocean13.628.134.8-22.70.00.366545433.024.00.540.030.410.100.000.410.10
M130_1002-1CTD/RosetteCTD-RO2016-09-07T12:129.99983-19.99900-4478Eastern Tropical Atlantic Ocean8.728.134.7-22.70.00.366445433.024.00.560.110.140.022.520.522.071.524.592.04
M130_1005-1 CTD/RosetteCTD-RO2016-09-08T04:1811.99950-20.99917-4918Eastern Tropical Atlantic Ocean39.020.135.8-23.50.00.367345433.024.00.560.050.130.060.000.000.130.06
M130_1005-1CTD/RosetteCTD-RO2016-09-08T04:1811.99950-20.99917-4918Eastern Tropical Atlantic Ocean18.528.035.7-23.10.00.367545433.024.00.460.010.532.980.930.633.610.93
M130_1005-1CTD/RosetteCTD-RO2016-09-08T04:1811.99950-20.99917-4918Eastern Tropical Atlantic Ocean9.028.635.7-21.73.70.366545433.024.10.380.092.580.450.000.002.580.45
M130_1010-1 CTD/RosetteCTD-RO2016-09-08T22:0811.02517-21.20867-5076Eastern Tropical Atlantic Ocean44.116.835.7-25.80.00.365245433.024.00.280.020.110.110.020.140.250.02
M130_1010-1CTD/RosetteCTD-RO2016-09-08T22:0811.02517-21.20867-5076Eastern Tropical Atlantic Ocean26.723.235.9-22.40.00.366945433.024.00.450.020.130.520.010.120.640.01
M130_1010-1CTD/RosetteCTD-RO2016-09-08T22:0811.02517-21.20867-5076Eastern Tropical Atlantic Ocean9.928.535.8-21.82.40.365345433.024.00.410.030.130.033.340.770.690.204.020.97
M130_1016-1 CTD/RosetteCTD-RO2016-09-09T14:459.00000-20.99983-2042Eastern Tropical Atlantic Ocean33.622.135.9-22.60.00.368845433.024.00.320.100.000.000.000.000.000.00
M130_1016-1CTD/RosetteCTD-RO2016-09-09T14:459.00000-20.99983-2042Eastern Tropical Atlantic Ocean26.924.136.0-22.70.00.366945433.024.00.490.030.130.250.090.080.330.09
M130_1016-1CTD/RosetteCTD-RO2016-09-09T14:459.00000-20.99983-2042Eastern Tropical Atlantic Ocean9.327.635.5-21.37.50.365445433.024.00.460.040.178.232.194.3812.612.19
M130_1019-1 CTD/RosetteCTD-RO2016-09-10T09:197.49967-22.99983-4392Eastern Tropical Atlantic Ocean9.627.835.3-21.28.70.365145433.024.00.310.044.120.150.000.004.120.15
M130_1027-1 CTD/RosetteCTD-RO2016-09-10T20:276.49917-23.00033-3088Eastern Tropical Atlantic Ocean8.528.035.3-21.19.80.364645433.024.00.300.023.940.920.000.003.940.92
M130_1031-1 CTD/RosetteCTD-RO2016-09-11T05:305.50017-23.00000-4233Eastern Tropical Atlantic Ocean9.127.935.2-21.55.90.365045433.024.00.330.012.820.210.000.002.820.21
M130_1037-1 CTD/RosetteCTD-RO2016-09-11T14:345.00017-22.99983-4217Eastern Tropical Atlantic Ocean8.627.935.0-21.47.10.364645433.024.00.260.012.350.520.000.002.350.52
M130_1042-1 CTD/RosetteCTD-RO2016-09-12T04:094.50017-22.99983-4130Eastern Tropical Atlantic Ocean9.227.834.9-22.40.00.364145433.024.00.280.042.140.270.000.002.140.27
M130_1045-1 CTD/RosetteCTD-RO2016-09-12T14:433.99933-22.99983-4220Eastern Tropical Atlantic Ocean8.827.735.2-21.90.60.364445433.024.00.270.022.020.400.000.002.020.40
M130_1047-1 CTD/RosetteCTD-RO2016-09-12T20:393.50033-22.99983-4388Eastern Tropical Atlantic Ocean8.927.635.6-21.82.40.365245433.024.00.240.022.200.440.000.002.200.44
M130_1050-1 CTD/RosetteCTD-RO2016-09-13T03:213.00050-23.00000-4650Eastern Tropical Atlantic Ocean8.927.436.0-21.55.30.365345433.024.00.320.062.980.810.000.002.980.81
M130_1051-1 CTD/RosetteCTD-RO2016-09-13T09:212.50050-23.00000-4714Eastern Tropical Atlantic Ocean9.526.836.0-22.70.00.365245433.024.00.320.031.890.080.000.001.890.08
M130_1054-1 CTD/RosetteCTD-RO2016-09-13T15:351.99800-23.00167-4332Eastern Tropical Atlantic Ocean8.926.735.9-22.80.00.366645433.024.00.290.012.010.250.000.002.010.25
M130_1056-1 CTD/RosetteCTD-RO2016-09-14T01:341.33367-23.00133-4720Eastern Tropical Atlantic Ocean9.326.136.0-22.50.00.367345433.024.10.340.022.100.230.000.002.100.23
M130_1060-1 CTD/RosetteCTD-RO2016-09-14T13:110.66817-22.99967-4314Eastern Tropical Atlantic Ocean9.226.036.1-22.70.00.366045433.024.00.410.022.270.370.000.002.270.37
M130_1068-1 CTD/RosetteCTD-RO2016-09-14T22:170.33317-22.99767-3918Eastern Tropical Atlantic Ocean71.524.236.1-23.40.00.366645433.023.90.220.010.050.010.070.000.000.000.070.00
M130_1068-1CTD/RosetteCTD-RO2016-09-14T22:170.33317-22.99767-3918Eastern Tropical Atlantic Ocean35.225.836.1-22.30.00.367645433.024.00.430.050.090.012.640.690.000.002.640.69
M130_1068-1CTD/RosetteCTD-RO2016-09-14T22:170.33317-22.99767-3918Eastern Tropical Atlantic Ocean7.426.036.1-22.50.00.367645433.024.00.490.090.122.320.190.112.430.19
M130_1069-1 CTD/RosetteCTD-RO2016-09-15T02:100.00250-23.03117-3949Eastern Tropical Atlantic Ocean8.925.936.1-22.90.00.366745433.024.00.350.062.440.320.000.002.440.32
M130_1078-1 CTD/RosetteCTD-RO2016-09-15T23:30-0.33217-22.99883-4621Eastern Tropical Atlantic Ocean9.526.036.2-21.81.90.367445433.024.00.360.041.910.380.000.001.910.38
M130_1080-1 CTD/RosetteCTD-RO2016-09-16T04:20-0.66567-22.99883-3570Eastern Tropical Atlantic Ocean9.325.936.2-22.70.00.366745433.024.00.470.082.430.240.000.002.430.24
M130_1090-1 CTD/RosetteCTD-RO2016-09-16T21:41-1.32850-22.99250-4837Eastern Tropical Atlantic Ocean56.425.336.1-22.90.00.367445433.023.10.370.040.100.020.000.100.02
M130_1090-1CTD/RosetteCTD-RO2016-09-16T21:41-1.32850-22.99250-4837Eastern Tropical Atlantic Ocean38.526.036.1-22.10.00.367545433.023.10.400.081.860.260.001.860.26
M130_1090-1CTD/RosetteCTD-RO2016-09-16T21:41-1.32850-22.99250-4837Eastern Tropical Atlantic Ocean9.226.036.1-21.91.00.367445433.023.10.390.032.390.440.002.390.44
M130_1092-1 CTD/RosetteCTD-RO2016-09-17T08:19-1.99967-23.00017-5235Eastern Tropical Atlantic Ocean8.925.836.1-21.011.50.360845433.024.00.300.042.240.400.000.002.240.40
M130_1094-1 CTD/RosetteCTD-RO2016-09-17T15:15-2.50300-23.00133-5776Eastern Tropical Atlantic Ocean9.625.936.1-22.40.00.367745433.024.00.330.012.230.240.000.002.230.24
M130_1096-1 CTD/RosetteCTD-RO2016-09-17T21:53-2.99983-23.00033-5487Eastern Tropical Atlantic Ocean10.325.836.1-23.30.00.368045433.024.00.310.012.280.150.000.002.280.15
M130_1100-1 CTD/RosetteCTD-RO2016-09-18T12:37-3.99883-23.00067-5860Eastern Tropical Atlantic Ocean8.525.836.1-22.60.00.367045433.024.00.330.041.780.280.000.001.780.28
M130_1104-1 CTD/RosetteCTD-RO2016-09-19T01:51-5.00000-23.00017-5306Eastern Tropical Atlantic Ocean8.625.836.0-22.90.00.367145433.024.00.290.021.700.400.000.001.700.40