PANGAEA_961210 KOSMOS 2020 Peru mesocosm study on ecosystem responses to different light and upwelling intensities: HPLC pigment concentrations and phytoplankton community composition based on CHEMTAX analysis of water samples Behncke, Jacqueline; Thielecke, Antonia; Kittu, Leila; Georgieva, Silvia; Nachtigall, Kerstin; Hansen, Levka; Fernández-Méndez, Mar KOSMOS 2020 Peru mesocosm study on ecosystem responses to different light and upwelling intensities: HPLC pigment concentrations and phytoplankton community composition based on CHEMTAX analysis of water samples 2023-11-02 PANGAEA Dataset https://doi.pangaea.de/10.1594/PANGAEA.961210 Investigator Jacqueline Behncke jacqueline.behncke@mpimet.mpg.de Earth Science Type of study Event label Mesocosm label DATE/TIME Day of experiment Depth, water, experiment, top/minimum Depth, water, experiment, bottom/maximum Treatment: light condition Treatment Chlorophyll a, Diatoms Chlorophyll a, Dinoflagellata Chlorophyll a, Cryptophyta Chlorophyll a, Pelagophytes Chlorophyll a, Coccolithophores Chlorophyll a, Phaeocystis Chlorophyll a, Prasinophyta Chlorophyll a, Chlorophyta Chlorophyll a, Synechococcus spp. Chlorophyll a, Fibrocapsa japonica Chlorophyll b Chlorophyll c3 Peridinin 19-Butanoyloxyfucoxanthin Fucoxanthin Neoxanthin Prasinoxanthin Violaxanthin 19-Hexanoyloxyfucoxanthin Alloxanthin Zeaxanthin Lutein Chlorophyll a geoscientificInformation Chemtax Field experiment HPLC pigments KOSMOS_2020 KOSMOS_2020_Mesocosm-M1 KOSMOS_2020_Mesocosm-M10 KOSMOS_2020_Mesocosm-M2 KOSMOS_2020_Mesocosm-M3 KOSMOS_2020_Mesocosm-M4 KOSMOS_2020_Mesocosm-M5 KOSMOS_2020_Mesocosm-M6 KOSMOS_2020_Mesocosm-M7 KOSMOS_2020_Mesocosm-M8 KOSMOS_2020_Mesocosm-M9 light limitation mesocosm experiment Mesocosm experiment Phytoplankton composition Upwelling HPLC/CHEMTAX (Mackey et al. 1996) 2020-02-28 2020-04-02 Complete -12.0561296 -12.0546899 -77.2356439 -77.2346773 CUSCO Coastal Upwelling System in a Changing Ocean AQUACOSM Network of Leading European AQUAtic MesoCOSM Facilities Connecting Mountains to Oceans from the Arctic to the Mediterranean unrestricted CC-BY-4.0: Creative Commons Attribution 4.0 International English PANGAEA Data Publisher for Earth & Environmental Science https://www.pangaea.de/ Data Center Contact Michael Diepenbroek info@pangaea.de

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Bremen Bremen 28359 Germany online 5484 data points text/tab-separated-values Thielecke, Antonia; Fernández-Méndez, Mar; Arístegui, Javier; Baumann, Moritz; Behncke, Jacqueline; Berger, Stella A; Dausmann, Veit; Georgieva, Silvia; Goldenberg, Silvan Urs; Gomez-Saez, Gonzalo V; Graco, Michelle; Heene, Toralf; Kittu, Leila; Krause, Jeffrey W; Ludwig, Andrea; Meyer, Jana; Mohrholz, Volker; Nejstgaard, Jens Christian; Ortiz Cortes, Joaquin; Schulz, Kai Georg; Smith, Alexandra; Spilling, Kristian; Sswat, Michael; Taucher, Jan; Vanharanta, Mari; Varmanen, Pia; Riebesell, Ulf (2025): Disentangling upwelling: how light and nutrient supply shape primary producers and stoichiometry in the Humboldt upwelling system. Deep Sea Research Part II: Topical Studies in Oceanography, 223, 105522, https://doi.org/10.1016/j.dsr2.2025.105522

This data is part of the BMBF project CUSCO (Coastal Upwelling Systems in a Changing Ocean). Here we report pigment concentrations and the phytoplankton community composition based on CHEMTAX analysis during a 35-day experiment, where we enclosed natural plankton communities in in-situ mesocosms off Peru. The experiment investigated the interactive effects of light and upwelling on the Humboldt upwelling ecosystem by mimicking a gradient of upwelling intensities (0%, 15%, 30%, 45% and 60%) under summer-time high light and winter-time low light. Integrated seawater samples from a depth between 0 and 10m were collected using a 5L Integrating Water sampler (IWS; Hydro-Bios, Kiel). To obtain pigment concentrations 0.15 – 1L of water sampled from the water column were filtered onto glass-fiber filters (precombusted at 450°C for 6h, GFF, 0.7 µm nominal pore size, Whatman) using a low vacuum of 200 mbar. Afterwards the filters were stored in cryovials at -80°C until analysis of photosynthetic pigments using reverse-phase high-performance liquid chromatography (HPLC) following (Barlow et al., 1997; doi:10.3354/meps161303) as described by (Paul et al., 2015; doi:10.5194/bg-19-5911-2022). The phytoplankton community composition was calculated with CHEMTAX, which classifies phytoplankton taxa based upon taxon-specific pigment ratios (Mackey et al., 1996; doi:10.3354/meps144265). The values for the Peruvian upwelling system determined by (DiTullio et al., 2005; doi:10.4319/LO.2005.50.6.1887) as described by (Meyer et al., 2017; doi:10.3389/fmars.2017.00001) were used as pigment ratios. As phytoplankton microscopy revealed high abundances of the raphydophite Fibrocapsa japonica, the initial pigment matrix was complemented by ratios for F. japonica from (Laza-Martinez et al., 2007; doi:10.1093/plankt/fbm069) from the Atlantic. ** For all details see the full metadata description at "https://doi.pangaea.de/10.1594/PANGAEA.961210"! ** Mesocosm 10 describes the control sample from the Pacific HPLC data for day3 - mesocosm3 was measured as triplicate and day 7 - mesocosm 9 as duplicate. The values provided are the averages of the measured replicates.

http://en.wikipedia.org/wiki/ISO_8601 DATE/TIME http://invabio.wdc-mare.org/mesocubi.jpg Mesocosm experiment https://doi.org/10.1016/j.dsr2.2025.105522 Disentangling upwelling: how light and nutrient supply shape primary producers and stoichiometry in the Humboldt upwelling system https://doi.org/10.3354/meps144265 HPLC/CHEMTAX (Mackey et al. 1996) https://www.aquacosm.eu/ AQUACOSM https://www.geomar.de/en/research/ongoing-projects/project-details/prj/352964 CUSCO DIF 9.4 2023-11-02 2026-04-30