10.1594/PANGAEA.961210Behncke, JacquelineJacquelineBehncke0000-0002-0910-3247GEOMAR - Helmholtz Centre for Ocean Research KielMax-Planck-Institut für Meteorologie, HamburgThielecke, AntoniaAntoniaThielecke0009-0005-9584-4699Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, BremerhavenGEOMAR - Helmholtz Centre for Ocean Research KielKittu, LeilaLeilaKittu0000-0002-3986-8179GEOMAR - Helmholtz Centre for Ocean Research KielGeorgieva, SilviaSilviaGeorgievaGEOMAR - Helmholtz Centre for Ocean Research KielNachtigall, KerstinKerstinNachtigallGEOMAR - Helmholtz Centre for Ocean Research KielHansen, LevkaLevkaHansenGEOMAR - Helmholtz Centre for Ocean Research KielFernández-Méndez, MarMarFernández-Méndez0000-0002-4968-1156Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, BremerhavenGEOMAR - Helmholtz Centre for Ocean Research KielPANGAEA2023ChemtaxField experimentHPLC pigmentslight limitationmesocosm experimentPhytoplankton compositionUpwellingType of studyEvent labelMesocosm labelDATE/TIMEDay of experimentDepth, water, experiment, top/minimumDepth, water, experiment, bottom/maximumTreatment: light conditionTreatmentChlorophyll a, DiatomsChlorophyll a, DinoflagellataChlorophyll a, CryptophytaChlorophyll a, PelagophytesChlorophyll a, CoccolithophoresChlorophyll a, PhaeocystisChlorophyll a, PrasinophytaChlorophyll a, ChlorophytaChlorophyll a, Synechococcus spp.Chlorophyll a, Fibrocapsa japonicaChlorophyll bChlorophyll c3Peridinin19-ButanoyloxyfucoxanthinFucoxanthinNeoxanthinPrasinoxanthinViolaxanthin19-HexanoyloxyfucoxanthinAlloxanthinZeaxanthinLuteinChlorophyll aMesocosm experimentHPLC/CHEMTAX (Mackey et al. 1996)KOSMOS_2020Coastal Upwelling System in a Changing Ocean (CUSCO)Network of Leading European AQUAtic MesoCOSM Facilities Connecting Mountains to Oceans from the Arctic to the Mediterranean (AQUACOSM)2020-02-28T00:00:00/2020-04-02T00:00:00Dataset10.1016/j.dsr2.2025.1055225484 data pointstext/tab-separated-valuesCreative Commons Attribution 4.0 InternationalThis data is part of the BMBF project CUSCO (Coastal Upwelling Systems in a Changing Ocean). Here we report pigment concentrations and the phytoplankton community composition based on CHEMTAX analysis during a 35-day experiment, where we enclosed natural plankton communities in in-situ mesocosms off Peru. The experiment investigated the interactive effects of light and upwelling on the Humboldt upwelling ecosystem by mimicking a gradient of upwelling intensities (0%, 15%, 30%, 45% and 60%) under summer-time high light and winter-time low light. Integrated seawater samples from a depth between 0 and 10m were collected using a 5L Integrating Water sampler (IWS; Hydro-Bios, Kiel). To obtain pigment concentrations 0.15 – 1L of water sampled from the water column were filtered onto glass-fiber filters (precombusted at 450°C for 6h, GFF, 0.7 µm nominal pore size, Whatman) using a low vacuum of 200 mbar. Afterwards the filters were stored in cryovials at -80°C until analysis of photosynthetic pigments using reverse-phase high-performance liquid chromatography (HPLC) following (Barlow et al., 1997; doi:10.3354/meps161303) as described by (Paul et al., 2015; doi:10.5194/bg-19-5911-2022). The phytoplankton community composition was calculated with CHEMTAX, which classifies phytoplankton taxa based upon taxon-specific pigment ratios (Mackey et al., 1996; doi:10.3354/meps144265). The values for the Peruvian upwelling system determined by (DiTullio et al., 2005; doi:10.4319/LO.2005.50.6.1887) as described by (Meyer et al., 2017; doi:10.3389/fmars.2017.00001) were used as pigment ratios. As phytoplankton microscopy revealed high abundances of the raphydophite Fibrocapsa japonica, the initial pigment matrix was complemented by ratios for F. japonica from (Laza-Martinez et al., 2007; doi:10.1093/plankt/fbm069) from the Atlantic.Mesocosm 10 describes the control sample from the Pacific
HPLC data for day3 - mesocosm3 was measured as triplicate and day 7 - mesocosm 9 as duplicate. The values provided are the averages of the measured replicates.-77.2356439-77.2346773-12.0561296-12.0546899Federal Ministry of Education and Researchhttps://doi.org/10.13039/50110000234703F0813AKüstennahe Auftriebsgebiete CUSCO: Auswirkungen geänderter Auftriebsintensitäten auf Struktur, Funktion und Produktivität pelagischer Nahrungsnetze im peruanischen Humboldt-AuftriebsgebietHorizon 2020https://doi.org/10.13039/501100007601731065Network of Leading European AQUAtic MesoCOSM Facilities Connecting Mountains to Oceans from the Arctic to the Mediterranean