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Thielecke, Antonia; Kittu, Leila; Fernández-Méndez, Mar; Georgieva, Silvia; Behncke, Jacqueline; Nachtigall, Kerstin; Hansen, Levka (2023): KOSMOS 2020 Peru mesocosm study on ecosystem responses to different light and upwelling intensities: carbon and nitrogen uptake rates from water samples [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.961202

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Abstract:
This data is part of the BMBF project CUSCO (Coastal Upwelling Systems in a Changing Ocean). Here we report carbon and nitrogen uptake rates by phytoplankton collected during a 35-day experiment where we enclosed natural plankton communities in in-situ mesocosms off Peru. The experiment investigated the interactive effects of light and upwelling on the Humboldt upwelling ecosystem by mimicking a gradient of upwelling intensities (0%, 15%, 30%, 45% and 60%) under summer-time high light and winter-time low light. Integrated seawater samples from a depth between 0 and 10m were collected using a 5L Integrating Water sampler (IWS; Hydro-Bios, Kiel). Samples were spiked with 13C labelled sodium bicarbonate and 15N labelled potassium nitrate. Samples were incubated for 24 hours at light intensities that resemble the light conditions in the mesocosms. After incubation a subsample (150-1000 ml) was filtered onto a 0.7 µm pre-combusted glass-fiber filters (GFF, Whatmann). The filters were acidified, then dried in the oven at 60 °C and later measured using an elemental analyzer (Flash IRMS, ThermoFisher) connected to a mass spectrometer (Delta V Advantage Isotope Ratio MS, ThermoFisher) with the ConFlo IV (ThermoFisher). Carbon and nitrogen uptake rates were calculated based on Slawyk et al. (1977, DOI:10.4319/lo.1977.22.5.0925) and Shiozaki et al. (2009, DOI:10.3354/meps07837) respectively.
Keyword(s):
carbon uptake; Field experiment; Humboldt Current System; light limitation; mesocosm study; nutrient uptake; Peru; Upwelling
Funding:
Federal Ministry of Education and Research (BMBF), grant/award no. 03F0813A: Küstennahe Auftriebsgebiete CUSCO: Auswirkungen geänderter Auftriebsintensitäten auf Struktur, Funktion und Produktivität pelagischer Nahrungsnetze im peruanischen Humboldt-Auftriebsgebiet
Horizon 2020 (H2020), grant/award no. 731065: Network of Leading European AQUAtic MesoCOSM Facilities Connecting Mountains to Oceans from the Arctic to the Mediterranean
Coverage:
Median Latitude: -12.055329 * Median Longitude: -77.235145 * South-bound Latitude: -12.056130 * West-bound Longitude: -77.235644 * North-bound Latitude: -12.054690 * East-bound Longitude: -77.234677
Date/Time Start: 2020-02-28T00:00:00 * Date/Time End: 2020-04-02T00:00:00
Event(s):
KOSMOS_2020_Mesocosm-M1 * Latitude: -12.054828 * Longitude: -77.235211 * Date/Time Start: 2020-02-28T00:00:00 * Date/Time End: 2020-04-02T00:00:00 * Campaign: KOSMOS_2020 (KOSMOS Peru) * Method/Device: Mesocosm experiment (MESO)
KOSMOS_2020_Mesocosm-M2 * Latitude: -12.054690 * Longitude: -77.234944 * Date/Time Start: 2020-02-28T00:00:00 * Date/Time End: 2020-04-02T00:00:00 * Campaign: KOSMOS_2020 (KOSMOS Peru) * Method/Device: Mesocosm experiment (MESO)
KOSMOS_2020_Mesocosm-M3 * Latitude: -12.054725 * Longitude: -77.234677 * Date/Time Start: 2020-02-28T00:00:00 * Date/Time End: 2020-04-02T00:00:00 * Campaign: KOSMOS_2020 (KOSMOS Peru) * Method/Device: Mesocosm experiment (MESO)
Comment:
Mesocosm 10 describes the control sample from the Pacific
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Type of studyStudy typeThielecke, Antonia
2Event labelEventThielecke, Antonia
3Mesocosm labelMeso labelThielecke, Antonia
4DATE/TIMEDate/TimeThielecke, AntoniaGeocode
5Day of experimentDOEdayThielecke, Antonia
6Depth, water, experiment, top/minimumDepth water exp topmThielecke, Antonia
7Depth, water, experiment, bottom/maximumDepth water exp maxmThielecke, Antonia
8Treatment: light conditionT:lightThielecke, Antoniasummer-time high light (HL) and winter-time low light (LL)
9TreatmentTreatThielecke, Antoniaupwelling intensity in %
10TreatmentTreatThielecke, Antonia
11Carbon, organic, particulatePOCµmol/lThielecke, Antonia
12Nitrogen, organic, particulatePONµmol/lThielecke, Antonia
13Atom percent, 13CAt perc 13C%Thielecke, AntoniaDelta V Advantage Isotope Ratio Mass spectrometer with ConFlo IV interface (ThermoFisher)13C-POC
14Atom percent, 15NAt perc 15N%Thielecke, AntoniaDelta V Advantage Isotope Ratio Mass spectrometer with ConFlo IV interface (ThermoFisher)15N-PON
15Carbon uptake rateC upt rateµmol/l/dayThielecke, AntoniaCalculated
16Nitrogen uptake rateN upt rateµmol/l/dayThielecke, AntoniaCalculated
17Carbon/Nitrogen uptake ratioC/N uptThielecke, AntoniaCalculated
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0) (License comes into effect after moratorium ends)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
2168 data points

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