Citation:

PANGAEA.961012

Name: Seawater carbonate chemistry and photosynthetic performance and N2 fixation in the marine cyanobacterium Trichodesmium
Published: 2023-07-19
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Bacteria; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Cyanobacteria; Growth/Morphology; Laboratory experiment; Laboratory strains; Not applicable; Other metabolic rates; Oxygen; Pelagos; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species; Trichodesmium erythraeum

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Abstract:

Effects of changed levels of dissolved O2 and CO2 on marine primary producers are of general concern with respect to ecological effects of ongoing ocean deoxygenation and acidification as well as upwelled seawaters. We investigated the response of the diazotroph Trichodesmium erythraeum IMS 101 after it had acclimated to lowered pO2 (~60 μM O2) and/or elevated pCO2 levels (HC, ~32 μM CO2) for about 20 generations. Our results showed that reduced O2 levels decreased dark respiration significantly, and increased the net photosynthetic rate by 66 and 89% under the ambient (AC, ~13 μM CO2) and the HC, respectively. The reduced pO2 enhanced the N2 fixation rate by ~139% under AC and only by 44% under HC, respectively. The N2 fixation quotient, the ratio of N2 fixed per O2 evolved, increased by 143% when pO2 decreased by 75% under the elevated pCO2. Meanwhile, particulate organic carbon and nitrogen quota increased simultaneously under reduced O2 levels, regardless of the pCO2 treatments. Nevertheless, changed levels of O2 and CO2 did not bring about significant changes in the specific growth rate of the diazotroph. Such inconsistency was attributed to the daytime positive and nighttime negative effects of both lowered pO2 and elevated pCO2 on the energy supply for growth. Our results suggest that Trichodesmium decrease its dark respiration by 5% and increase its N2-fixation by 49% and N2-fixation quotient by 30% under future ocean deoxygenation and acidification with 16% decline of pO2 and 138% rise of pCO2 by the end of this century.

Keyword(s):

Bacteria; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Cyanobacteria; Growth/Morphology; Laboratory experiment; Laboratory strains; Not applicable; Other metabolic rates; Oxygen; Pelagos; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species; Trichodesmium erythraeum

Supplement to:

Li, He; Gao, Kunshan (2023): Deoxygenation enhances photosynthetic performance and increases N2 fixation in the marine cyanobacterium Trichodesmium under elevated pCO2. Frontiers in Microbiology, 14, 1102909, https://doi.org/10.3389/fmicb.2023.1102909

Documentation:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2019): seacarb: Seawater Carbonate Chemistry. R package version 3.2.15. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-07-19.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type of study	Study type		Gao, Kunshan
2	Species	Species		Gao, Kunshan
3	Treatment	Treat		Gao, Kunshan
4	Replicate	Repl		Gao, Kunshan
5	Growth rate	µ	1/day	Gao, Kunshan
6	Net photosynthesis rate, oxygen, per chlorophyll a	PN O2/Chl a	µmol/µg/h	Gao, Kunshan
7	Respiration rate, oxygen, per chlorophyll a	Resp O2/chl a	µmol/µg/h	Gao, Kunshan
8	Gross photosynthesis rate, oxygen	PG O2	µmol/g/h	Gao, Kunshan		per Chl a
9	Nitrogen fixation rate per chlorophyll a	N2 fix/Chl	nmol/µg/h	Gao, Kunshan
10	Ratio	Ratio		Gao, Kunshan		Nitrogen fixation quotient (N2 fixation/gross O2 evolution)
11	Oxygen	O2	µmol/l	Gao, Kunshan
12	Carbon, organic, particulate per chlorophyll a	POC/Chl a	µmol/mg	Gao, Kunshan
13	Nitrogen, organic, particulate per chlorophyll a	PON/Chl a	µmol/mg	Gao, Kunshan
14	Carbon/Nitrogen ratio	C/N		Gao, Kunshan
15	Particulate organic carbon production, per chlorophyll a	POC prod/Chl a	µmol/µg/day	Gao, Kunshan
16	Particulate organic nitrogen production, per chlorophyll a	PON prod/Chl a	µmol/µg/day	Gao, Kunshan
17	Salinity	Sal		Gao, Kunshan
18	Temperature, water	Temp	°C	Gao, Kunshan
19	Oxygen	O2	µmol/l	Gao, Kunshan		initial
20	Oxygen, standard deviation	O2 std dev	±	Gao, Kunshan		initial
21	pH	pH		Gao, Kunshan		NBS scale, initial
22	pH, standard deviation	pH std dev	±	Gao, Kunshan		NBS scale, initial
23	Alkalinity, total	AT	µmol/l	Gao, Kunshan		Initial
24	Alkalinity, total, standard deviation	AT std dev	±	Gao, Kunshan		Initial
25	Oxygen	O2	µmol/l	Gao, Kunshan		end
26	Oxygen, standard deviation	O2 std dev	±	Gao, Kunshan		end
27	pH	pH		Gao, Kunshan		NBS scale, end
28	pH, standard deviation	pH std dev	±	Gao, Kunshan		NBS scale, end
29	Alkalinity, total	AT	µmol/l	Gao, Kunshan		end
30	Alkalinity, total, standard deviation	AT std dev	±	Gao, Kunshan		end
31	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
32	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale, initial
33	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
34	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
35	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
36	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
37	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
38	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
39	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
40	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
41	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
42	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale, end
43	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	end
44	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	end
45	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	end
46	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	end
47	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	end
48	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	end
49	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	end
50	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	end
51	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	end

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

3012 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Study type	2 Species	3 Treat	4 Repl	5 µ [1/day]	6 PN O2/Chl a [µmol/µg/h]	7 Resp O2/chl a [µmol/µg/h]	8 PG O2 [µmol/g/h] (per Chl a)	9 N2 fix/Chl [nmol/µg/h]	10 Ratio (Nitrogen fixation quotient (N...)	11 O2 [µmol/l]	12 POC/Chl a [µmol/mg]	13 PON/Chl a [µmol/mg]	14 C/N	15 POC prod/Chl a [µmol/µg/day]	16 PON prod/Chl a [µmol/µg/day]	17 Sal	18 Temp [°C]	19 O2 [µmol/l] (initial)	20 O2 std dev [±] (initial)	21 pH (NBS scale, initial)	22 pH std dev [±] (NBS scale, initial)	23 AT [µmol/l] (Initial)	24 AT std dev [±] (Initial)	25 O2 [µmol/l] (end)	26 O2 std dev [±] (end)	27 pH (NBS scale, end)	28 pH std dev [±] (NBS scale, end)	29 AT [µmol/l] (end)	30 AT std dev [±] (end)	31 CSC flag	32 pH (total scale, initial)	33 CO2 [µmol/kg] (initial)	34 fCO2water_SST_wet [µatm] (initial)	35 pCO2water_SST_wet [µatm] (initial)	36 [HCO3]- [µmol/kg] (initial)	37 [CO3]2- [µmol/kg] (initial)	38 DIC [µmol/kg] (initial)	39 AT [µmol/kg] (initial)	40 Omega Arg (initial)	41 Omega Cal (initial)	42 pH (total scale, end)	43 CO2 [µmol/kg] (end)	44 fCO2water_SST_wet [µatm] (end)	45 pCO2water_SST_wet [µatm] (end)	46 [HCO3]- [µmol/kg] (end)	47 [CO3]2- [µmol/kg] (end)	48 DIC [µmol/kg] (end)	49 AT [µmol/kg] (end)	50 Omega Arg (end)	51 Omega Cal (end)
laboratory	Trichodesmium erythraeum	ACAO	1	0.5247												32	27	248.7	1.5	8.17	0.00	2368.9	32.8	256.7	1.5	8.20	0.01	2394.1	12.0	26	8.03	11.89	433.52	434.88	1815.52	208.79	2036.2	2321.4	3.41	5.17	8.06	11.05	402.73	403.99	1807.32	222.73	2041.1	2346.1	3.64	5.52
laboratory	Trichodesmium erythraeum	HCAO	1	0.5192												32	27	245.0	1.0	7.79	0.01	2373.5	19.1	253.3	1.5	7.86	0.02	2350.9	55.4	26	7.65	32.75	1193.74	1197.48	2084.40	99.95	2217.1	2325.9	1.63	2.48	7.72	27.11	988.16	991.25	2027.27	114.21	2168.6	2303.8	1.86	2.83
laboratory	Trichodesmium erythraeum	ACLO	1	0.4880												32	27	60.2	0.8	8.15	0.01	2365.5	19.3	89.2	1.6	8.21	0.01	2402.0	36.4	26	8.01	12.55	457.56	458.99	1830.70	201.14	2044.4	2318.1	3.28	4.98	8.07	10.78	392.86	394.09	1803.87	227.45	2042.1	2353.8	3.71	5.64
laboratory	Trichodesmium erythraeum	HCLO	1	0.4885												32	27	61.9	1.0	7.81	0.01	2390.1	18.3	91.3	2.8	7.90	0.01	2409.2	17.0	26	7.67	31.35	1142.69	1146.27	2089.07	104.88	2225.3	2342.2	1.71	2.60	7.76	25.06	913.29	916.14	2054.79	126.95	2206.8	2360.9	2.07	3.15
laboratory	Trichodesmium erythraeum	ACAO	2	0.5317												32	27	248.7	1.5	8.17	0.00	2368.9	32.8	256.7	1.5	8.20	0.01	2394.1	12.0	26	8.03	11.89	433.52	434.88	1815.52	208.79	2036.2	2321.4	3.41	5.17	8.06	11.05	402.73	403.99	1807.32	222.73	2041.1	2346.1	3.64	5.52
laboratory	Trichodesmium erythraeum	HCAO	2	0.5252												32	27	245.0	1.0	7.79	0.01	2373.5	19.1	253.3	1.5	7.86	0.02	2350.9	55.4	26	7.65	32.75	1193.74	1197.48	2084.40	99.95	2217.1	2325.9	1.63	2.48	7.72	27.11	988.16	991.25	2027.27	114.21	2168.6	2303.8	1.86	2.83
laboratory	Trichodesmium erythraeum	ACLO	2	0.4937												32	27	60.2	0.8	8.15	0.01	2365.5	19.3	89.2	1.6	8.21	0.01	2402.0	36.4	26	8.01	12.55	457.56	458.99	1830.70	201.14	2044.4	2318.1	3.28	4.98	8.07	10.78	392.86	394.09	1803.87	227.45	2042.1	2353.8	3.71	5.64
laboratory	Trichodesmium erythraeum	HCLO	2	0.5027												32	27	61.9	1.0	7.81	0.01	2390.1	18.3	91.3	2.8	7.90	0.01	2409.2	17.0	26	7.67	31.35	1142.69	1146.27	2089.07	104.88	2225.3	2342.2	1.71	2.60	7.76	25.06	913.29	916.14	2054.79	126.95	2206.8	2360.9	2.07	3.15
laboratory	Trichodesmium erythraeum	ACAO	3	0.5178												32	27	248.7	1.5	8.17	0.00	2368.9	32.8	256.7	1.5	8.20	0.01	2394.1	12.0	26	8.03	11.89	433.52	434.88	1815.52	208.79	2036.2	2321.4	3.41	5.17	8.06	11.05	402.73	403.99	1807.32	222.73	2041.1	2346.1	3.64	5.52
laboratory	Trichodesmium erythraeum	HCAO	3	0.5222												32	27	245.0	1.0	7.79	0.01	2373.5	19.1	253.3	1.5	7.86	0.02	2350.9	55.4	26	7.65	32.75	1193.74	1197.48	2084.40	99.95	2217.1	2325.9	1.63	2.48	7.72	27.11	988.16	991.25	2027.27	114.21	2168.6	2303.8	1.86	2.83
laboratory	Trichodesmium erythraeum	ACLO	3	0.4963												32	27	60.2	0.8	8.15	0.01	2365.5	19.3	89.2	1.6	8.21	0.01	2402.0	36.4	26	8.01	12.55	457.56	458.99	1830.70	201.14	2044.4	2318.1	3.28	4.98	8.07	10.78	392.86	394.09	1803.87	227.45	2042.1	2353.8	3.71	5.64
laboratory	Trichodesmium erythraeum	HCLO	3	0.4913												32	27	61.9	1.0	7.81	0.01	2390.1	18.3	91.3	2.8	7.90	0.01	2409.2	17.0	26	7.67	31.35	1142.69	1146.27	2089.07	104.88	2225.3	2342.2	1.71	2.60	7.76	25.06	913.29	916.14	2054.79	126.95	2206.8	2360.9	2.07	3.15
laboratory	Trichodesmium erythraeum	ACAO	4	0.5347												32	27	248.7	1.5	8.17	0.00	2368.9	32.8	256.7	1.5	8.20	0.01	2394.1	12.0	26	8.03	11.89	433.52	434.88	1815.52	208.79	2036.2	2321.4	3.41	5.17	8.06	11.05	402.73	403.99	1807.32	222.73	2041.1	2346.1	3.64	5.52
laboratory	Trichodesmium erythraeum	HCAO	4	0.5471												32	27	245.0	1.0	7.79	0.01	2373.5	19.1	253.3	1.5	7.86	0.02	2350.9	55.4	26	7.65	32.75	1193.74	1197.48	2084.40	99.95	2217.1	2325.9	1.63	2.48	7.72	27.11	988.16	991.25	2027.27	114.21	2168.6	2303.8	1.86	2.83
laboratory	Trichodesmium erythraeum	ACLO	4	0.5532												32	27	60.2	0.8	8.15	0.01	2365.5	19.3	89.2	1.6	8.21	0.01	2402.0	36.4	26	8.01	12.55	457.56	458.99	1830.70	201.14	2044.4	2318.1	3.28	4.98	8.07	10.78	392.86	394.09	1803.87	227.45	2042.1	2353.8	3.71	5.64
laboratory	Trichodesmium erythraeum	HCLO	4	0.5551												32	27	61.9	1.0	7.81	0.01	2390.1	18.3	91.3	2.8	7.90	0.01	2409.2	17.0	26	7.67	31.35	1142.69	1146.27	2089.07	104.88	2225.3	2342.2	1.71	2.60	7.76	25.06	913.29	916.14	2054.79	126.95	2206.8	2360.9	2.07	3.15
laboratory	Trichodesmium erythraeum	ACAO	5	0.5410												32	27	248.7	1.5	8.17	0.00	2368.9	32.8	256.7	1.5	8.20	0.01	2394.1	12.0	26	8.03	11.89	433.52	434.88	1815.52	208.79	2036.2	2321.4	3.41	5.17	8.06	11.05	402.73	403.99	1807.32	222.73	2041.1	2346.1	3.64	5.52
laboratory	Trichodesmium erythraeum	HCAO	5	0.5079												32	27	245.0	1.0	7.79	0.01	2373.5	19.1	253.3	1.5	7.86	0.02	2350.9	55.4	26	7.65	32.75	1193.74	1197.48	2084.40	99.95	2217.1	2325.9	1.63	2.48	7.72	27.11	988.16	991.25	2027.27	114.21	2168.6	2303.8	1.86	2.83
laboratory	Trichodesmium erythraeum	ACLO	5	0.5746												32	27	60.2	0.8	8.15	0.01	2365.5	19.3	89.2	1.6	8.21	0.01	2402.0	36.4	26	8.01	12.55	457.56	458.99	1830.70	201.14	2044.4	2318.1	3.28	4.98	8.07	10.78	392.86	394.09	1803.87	227.45	2042.1	2353.8	3.71	5.64
laboratory	Trichodesmium erythraeum	HCLO	5	0.5636												32	27	61.9	1.0	7.81	0.01	2390.1	18.3	91.3	2.8	7.90	0.01	2409.2	17.0	26	7.67	31.35	1142.69	1146.27	2089.07	104.88	2225.3	2342.2	1.71	2.60	7.76	25.06	913.29	916.14	2054.79	126.95	2206.8	2360.9	2.07	3.15
laboratory	Trichodesmium erythraeum	ACAO	6	0.5526												32	27	248.7	1.5	8.17	0.00	2368.9	32.8	256.7	1.5	8.20	0.01	2394.1	12.0	26	8.03	11.89	433.52	434.88	1815.52	208.79	2036.2	2321.4	3.41	5.17	8.06	11.05	402.73	403.99	1807.32	222.73	2041.1	2346.1	3.64	5.52
laboratory	Trichodesmium erythraeum	HCAO	6	0.5256												32	27	245.0	1.0	7.79	0.01	2373.5	19.1	253.3	1.5	7.86	0.02	2350.9	55.4	26	7.65	32.75	1193.74	1197.48	2084.40	99.95	2217.1	2325.9	1.63	2.48	7.72	27.11	988.16	991.25	2027.27	114.21	2168.6	2303.8	1.86	2.83
laboratory	Trichodesmium erythraeum	ACLO	6	0.5783												32	27	60.2	0.8	8.15	0.01	2365.5	19.3	89.2	1.6	8.21	0.01	2402.0	36.4	26	8.01	12.55	457.56	458.99	1830.70	201.14	2044.4	2318.1	3.28	4.98	8.07	10.78	392.86	394.09	1803.87	227.45	2042.1	2353.8	3.71	5.64
laboratory	Trichodesmium erythraeum	HCLO	6	0.5666												32	27	61.9	1.0	7.81	0.01	2390.1	18.3	91.3	2.8	7.90	0.01	2409.2	17.0	26	7.67	31.35	1142.69	1146.27	2089.07	104.88	2225.3	2342.2	1.71	2.60	7.76	25.06	913.29	916.14	2054.79	126.95	2206.8	2360.9	2.07	3.15
laboratory	Trichodesmium erythraeum	ACAO	1		0.343220362	0.221950059	565170.4202									32	27	248.7	1.5	8.17	0.00	2368.9	32.8	256.7	1.5	8.20	0.01	2394.1	12.0	26	8.03	11.89	433.52	434.88	1815.52	208.79	2036.2	2321.4	3.41	5.17	8.06	11.05	402.73	403.99	1807.32	222.73	2041.1	2346.1	3.64	5.52
laboratory	Trichodesmium erythraeum	HCAO	1		0.337140437	0.302377175	639517.6123									32	27	245.0	1.0	7.79	0.01	2373.5	19.1	253.3	1.5	7.86	0.02	2350.9	55.4	26	7.65	32.75	1193.74	1197.48	2084.40	99.95	2217.1	2325.9	1.63	2.48	7.72	27.11	988.16	991.25	2027.27	114.21	2168.6	2303.8	1.86	2.83
laboratory	Trichodesmium erythraeum	ACLO	1		0.643981459	0.137011903	780993.3618									32	27	60.2	0.8	8.15	0.01	2365.5	19.3	89.2	1.6	8.21	0.01	2402.0	36.4	26	8.01	12.55	457.56	458.99	1830.70	201.14	2044.4	2318.1	3.28	4.98	8.07	10.78	392.86	394.09	1803.87	227.45	2042.1	2353.8	3.71	5.64
laboratory	Trichodesmium erythraeum	HCLO	1		0.602457868	0.156013307	758471.1749									32	27	61.9	1.0	7.81	0.01	2390.1	18.3	91.3	2.8	7.90	0.01	2409.2	17.0	26	7.67	31.35	1142.69	1146.27	2089.07	104.88	2225.3	2342.2	1.71	2.60	7.76	25.06	913.29	916.14	2054.79	126.95	2206.8	2360.9	2.07	3.15
laboratory	Trichodesmium erythraeum	ACAO	2		0.396511875	0.203839128	600351.0036									32	27	248.7	1.5	8.17	0.00	2368.9	32.8	256.7	1.5	8.20	0.01	2394.1	12.0	26	8.03	11.89	433.52	434.88	1815.52	208.79	2036.2	2321.4	3.41	5.17	8.06	11.05	402.73	403.99	1807.32	222.73	2041.1	2346.1	3.64	5.52
laboratory	Trichodesmium erythraeum	HCAO	2		0.339931222	0.269228095	609159.3174									32	27	245.0	1.0	7.79	0.01	2373.5	19.1	253.3	1.5	7.86	0.02	2350.9	55.4	26	7.65	32.75	1193.74	1197.48	2084.40	99.95	2217.1	2325.9	1.63	2.48	7.72	27.11	988.16	991.25	2027.27	114.21	2168.6	2303.8	1.86	2.83
laboratory	Trichodesmium erythraeum	ACLO	2		0.647157374	0.134118908	781276.2827									32	27	60.2	0.8	8.15	0.01	2365.5	19.3	89.2	1.6	8.21	0.01	2402.0	36.4	26	8.01	12.55	457.56	458.99	1830.70	201.14	2044.4	2318.1	3.28	4.98	8.07	10.78	392.86	394.09	1803.87	227.45	2042.1	2353.8	3.71	5.64
laboratory	Trichodesmium erythraeum	HCLO	2		0.714320661	0.182056492	896377.1531									32	27	61.9	1.0	7.81	0.01	2390.1	18.3	91.3	2.8	7.90	0.01	2409.2	17.0	26	7.67	31.35	1142.69	1146.27	2089.07	104.88	2225.3	2342.2	1.71	2.60	7.76	25.06	913.29	916.14	2054.79	126.95	2206.8	2360.9	2.07	3.15
laboratory	Trichodesmium erythraeum	ACAO	3		0.397619795	0.252573081	650192.8762									32	27	248.7	1.5	8.17	0.00	2368.9	32.8	256.7	1.5	8.20	0.01	2394.1	12.0	26	8.03	11.89	433.52	434.88	1815.52	208.79	2036.2	2321.4	3.41	5.17	8.06	11.05	402.73	403.99	1807.32	222.73	2041.1	2346.1	3.64	5.52
laboratory	Trichodesmium erythraeum	HCAO	3		0.367730133	0.277562922	645293.0543									32	27	245.0	1.0	7.79	0.01	2373.5	19.1	253.3	1.5	7.86	0.02	2350.9	55.4	26	7.65	32.75	1193.74	1197.48	2084.40	99.95	2217.1	2325.9	1.63	2.48	7.72	27.11	988.16	991.25	2027.27	114.21	2168.6	2303.8	1.86	2.83
laboratory	Trichodesmium erythraeum	ACLO	3		0.602397672	0.136694113	739091.7849									32	27	60.2	0.8	8.15	0.01	2365.5	19.3	89.2	1.6	8.21	0.01	2402.0	36.4	26	8.01	12.55	457.56	458.99	1830.70	201.14	2044.4	2318.1	3.28	4.98	8.07	10.78	392.86	394.09	1803.87	227.45	2042.1	2353.8	3.71	5.64
laboratory	Trichodesmium erythraeum	HCLO	3		0.660299477	0.179283931	839583.4079									32	27	61.9	1.0	7.81	0.01	2390.1	18.3	91.3	2.8	7.90	0.01	2409.2	17.0	26	7.67	31.35	1142.69	1146.27	2089.07	104.88	2225.3	2342.2	1.71	2.60	7.76	25.06	913.29	916.14	2054.79	126.95	2206.8	2360.9	2.07	3.15
laboratory	Trichodesmium erythraeum	ACAO	1					7.477557831	0.013230625							32	27	248.7	1.5	8.17	0.00	2368.9	32.8	256.7	1.5	8.20	0.01	2394.1	12.0	26	8.03	11.89	433.52	434.88	1815.52	208.79	2036.2	2321.4	3.41	5.17	8.06	11.05	402.73	403.99	1807.32	222.73	2041.1	2346.1	3.64	5.52
laboratory	Trichodesmium erythraeum	HCAO	1					18.317063620	0.028642000							32	27	245.0	1.0	7.79	0.01	2373.5	19.1	253.3	1.5	7.86	0.02	2350.9	55.4	26	7.65	32.75	1193.74	1197.48	2084.40	99.95	2217.1	2325.9	1.63	2.48	7.72	27.11	988.16	991.25	2027.27	114.21	2168.6	2303.8	1.86	2.83
laboratory	Trichodesmium erythraeum	ACLO	1					20.997570880	0.026885723							32	27	60.2	0.8	8.15	0.01	2365.5	19.3	89.2	1.6	8.21	0.01	2402.0	36.4	26	8.01	12.55	457.56	458.99	1830.70	201.14	2044.4	2318.1	3.28	4.98	8.07	10.78	392.86	394.09	1803.87	227.45	2042.1	2353.8	3.71	5.64
laboratory	Trichodesmium erythraeum	HCLO	1					29.294136810	0.038622611							32	27	61.9	1.0	7.81	0.01	2390.1	18.3	91.3	2.8	7.90	0.01	2409.2	17.0	26	7.67	31.35	1142.69	1146.27	2089.07	104.88	2225.3	2342.2	1.71	2.60	7.76	25.06	913.29	916.14	2054.79	126.95	2206.8	2360.9	2.07	3.15
laboratory	Trichodesmium erythraeum	ACAO	2					7.656018221	0.012752570							32	27	248.7	1.5	8.17	0.00	2368.9	32.8	256.7	1.5	8.20	0.01	2394.1	12.0	26	8.03	11.89	433.52	434.88	1815.52	208.79	2036.2	2321.4	3.41	5.17	8.06	11.05	402.73	403.99	1807.32	222.73	2041.1	2346.1	3.64	5.52
laboratory	Trichodesmium erythraeum	HCAO	2					18.587208970	0.030512886							32	27	245.0	1.0	7.79	0.01	2373.5	19.1	253.3	1.5	7.86	0.02	2350.9	55.4	26	7.65	32.75	1193.74	1197.48	2084.40	99.95	2217.1	2325.9	1.63	2.48	7.72	27.11	988.16	991.25	2027.27	114.21	2168.6	2303.8	1.86	2.83
laboratory	Trichodesmium erythraeum	ACLO	2					19.810821980	0.025356999							32	27	60.2	0.8	8.15	0.01	2365.5	19.3	89.2	1.6	8.21	0.01	2402.0	36.4	26	8.01	12.55	457.56	458.99	1830.70	201.14	2044.4	2318.1	3.28	4.98	8.07	10.78	392.86	394.09	1803.87	227.45	2042.1	2353.8	3.71	5.64
laboratory	Trichodesmium erythraeum	HCLO	2					27.426060970	0.030596564							32	27	61.9	1.0	7.81	0.01	2390.1	18.3	91.3	2.8	7.90	0.01	2409.2	17.0	26	7.67	31.35	1142.69	1146.27	2089.07	104.88	2225.3	2342.2	1.71	2.60	7.76	25.06	913.29	916.14	2054.79	126.95	2206.8	2360.9	2.07	3.15
laboratory	Trichodesmium erythraeum	ACAO	3					9.935646142	0.015281075							32	27	248.7	1.5	8.17	0.00	2368.9	32.8	256.7	1.5	8.20	0.01	2394.1	12.0	26	8.03	11.89	433.52	434.88	1815.52	208.79	2036.2	2321.4	3.41	5.17	8.06	11.05	402.73	403.99	1807.32	222.73	2041.1	2346.1	3.64	5.52
laboratory	Trichodesmium erythraeum	HCAO	3					20.601298210	0.031925492							32	27	245.0	1.0	7.79	0.01	2373.5	19.1	253.3	1.5	7.86	0.02	2350.9	55.4	26	7.65	32.75	1193.74	1197.48	2084.40	99.95	2217.1	2325.9	1.63	2.48	7.72	27.11	988.16	991.25	2027.27	114.21	2168.6	2303.8	1.86	2.83
laboratory	Trichodesmium erythraeum	ACLO	3					19.104644170	0.025848811							32	27	60.2	0.8	8.15	0.01	2365.5	19.3	89.2	1.6	8.21	0.01	2402.0	36.4	26	8.01	12.55	457.56	458.99	1830.70	201.14	2044.4	2318.1	3.28	4.98	8.07	10.78	392.86	394.09	1803.87	227.45	2042.1	2353.8	3.71	5.64
laboratory	Trichodesmium erythraeum	HCLO	3					25.905020840	0.030854613							32	27	61.9	1.0	7.81	0.01	2390.1	18.3	91.3	2.8	7.90	0.01	2409.2	17.0	26	7.67	31.35	1142.69	1146.27	2089.07	104.88	2225.3	2342.2	1.71	2.60	7.76	25.06	913.29	916.14	2054.79	126.95	2206.8	2360.9	2.07	3.15
laboratory	Trichodesmium erythraeum		1					10.290990000		240.00
laboratory	Trichodesmium erythraeum		1					15.151530000		176.25
laboratory	Trichodesmium erythraeum		1					14.713680000		115.00
laboratory	Trichodesmium erythraeum		1					17.805220000		55.00
laboratory	Trichodesmium erythraeum		2					10.672040000		240.00
laboratory	Trichodesmium erythraeum		2					11.912010000		176.25
laboratory	Trichodesmium erythraeum		2					14.853760000		115.00
laboratory	Trichodesmium erythraeum		2					15.499890000		55.00
laboratory	Trichodesmium erythraeum		3					10.495570000		240.00
laboratory	Trichodesmium erythraeum		3					11.665080000		176.25
laboratory	Trichodesmium erythraeum		3					16.014130000		115.00
laboratory	Trichodesmium erythraeum		3					19.892380000		55.00
laboratory	Trichodesmium erythraeum	ACAO	1								9629.832465	1378.143481	6.987539831			32	27	248.7	1.5	8.17	0.00	2368.9	32.8	256.7	1.5	8.20	0.01	2394.1	12.0	26	8.03	11.89	433.52	434.88	1815.52	208.79	2036.2	2321.4	3.41	5.17	8.06	11.05	402.73	403.99	1807.32	222.73	2041.1	2346.1	3.64	5.52
laboratory	Trichodesmium erythraeum	HCAO	1								10098.303930	1479.234827	6.826707796			32	27	245.0	1.0	7.79	0.01	2373.5	19.1	253.3	1.5	7.86	0.02	2350.9	55.4	26	7.65	32.75	1193.74	1197.48	2084.40	99.95	2217.1	2325.9	1.63	2.48	7.72	27.11	988.16	991.25	2027.27	114.21	2168.6	2303.8	1.86	2.83
laboratory	Trichodesmium erythraeum	ACLO	1								10827.762210	1671.524709	6.477775743			32	27	60.2	0.8	8.15	0.01	2365.5	19.3	89.2	1.6	8.21	0.01	2402.0	36.4	26	8.01	12.55	457.56	458.99	1830.70	201.14	2044.4	2318.1	3.28	4.98	8.07	10.78	392.86	394.09	1803.87	227.45	2042.1	2353.8	3.71	5.64
laboratory	Trichodesmium erythraeum	HCLO	1								13945.666380	2020.875724	6.900803555			32	27	61.9	1.0	7.81	0.01	2390.1	18.3	91.3	2.8	7.90	0.01	2409.2	17.0	26	7.67	31.35	1142.69	1146.27	2089.07	104.88	2225.3	2342.2	1.71	2.60	7.76	25.06	913.29	916.14	2054.79	126.95	2206.8	2360.9	2.07	3.15
laboratory	Trichodesmium erythraeum	ACAO	2								9329.079317	1500.845814	6.215881223			32	27	248.7	1.5	8.17	0.00	2368.9	32.8	256.7	1.5	8.20	0.01	2394.1	12.0	26	8.03	11.89	433.52	434.88	1815.52	208.79	2036.2	2321.4	3.41	5.17	8.06	11.05	402.73	403.99	1807.32	222.73	2041.1	2346.1	3.64	5.52
laboratory	Trichodesmium erythraeum	HCAO	2								9895.275131	1397.419999	7.081103132			32	27	245.0	1.0	7.79	0.01	2373.5	19.1	253.3	1.5	7.86	0.02	2350.9	55.4	26	7.65	32.75	1193.74	1197.48	2084.40	99.95	2217.1	2325.9	1.63	2.48	7.72	27.11	988.16	991.25	2027.27	114.21	2168.6	2303.8	1.86	2.83
laboratory	Trichodesmium erythraeum	ACLO	2								10779.983890	1638.275573	6.580079728			32	27	60.2	0.8	8.15	0.01	2365.5	19.3	89.2	1.6	8.21	0.01	2402.0	36.4	26	8.01	12.55	457.56	458.99	1830.70	201.14	2044.4	2318.1	3.28	4.98	8.07	10.78	392.86	394.09	1803.87	227.45	2042.1	2353.8	3.71	5.64
laboratory	Trichodesmium erythraeum	HCLO	2								12681.162950	1911.603616	6.633782674			32	27	61.9	1.0	7.81	0.01	2390.1	18.3	91.3	2.8	7.90	0.01	2409.2	17.0	26	7.67	31.35	1142.69	1146.27	2089.07	104.88	2225.3	2342.2	1.71	2.60	7.76	25.06	913.29	916.14	2054.79	126.95	2206.8	2360.9	2.07	3.15
laboratory	Trichodesmium erythraeum	ACAO	3								9826.755595	1497.002250	6.564289131			32	27	248.7	1.5	8.17	0.00	2368.9	32.8	256.7	1.5	8.20	0.01	2394.1	12.0	26	8.03	11.89	433.52	434.88	1815.52	208.79	2036.2	2321.4	3.41	5.17	8.06	11.05	402.73	403.99	1807.32	222.73	2041.1	2346.1	3.64	5.52
laboratory	Trichodesmium erythraeum	HCAO	3								9393.551682	1373.818324	6.837550148			32	27	245.0	1.0	7.79	0.01	2373.5	19.1	253.3	1.5	7.86	0.02	2350.9	55.4	26	7.65	32.75	1193.74	1197.48	2084.40	99.95	2217.1	2325.9	1.63	2.48	7.72	27.11	988.16	991.25	2027.27	114.21	2168.6	2303.8	1.86	2.83
laboratory	Trichodesmium erythraeum	ACLO	3								11716.690460	1721.637042	6.805552030			32	27	60.2	0.8	8.15	0.01	2365.5	19.3	89.2	1.6	8.21	0.01	2402.0	36.4	26	8.01	12.55	457.56	458.99	1830.70	201.14	2044.4	2318.1	3.28	4.98	8.07	10.78	392.86	394.09	1803.87	227.45	2042.1	2353.8	3.71	5.64
laboratory	Trichodesmium erythraeum	HCLO	3								15728.511710	2215.526526	7.099220670			32	27	61.9	1.0	7.81	0.01	2390.1	18.3	91.3	2.8	7.90	0.01	2409.2	17.0	26	7.67	31.35	1142.69	1146.27	2089.07	104.88	2225.3	2342.2	1.71	2.60	7.76	25.06	913.29	916.14	2054.79	126.95	2206.8	2360.9	2.07	3.15
laboratory	Trichodesmium erythraeum	ACAO	1											5.052773095	0.723111884	32	27	248.7	1.5	8.17	0.00	2368.9	32.8	256.7	1.5	8.20	0.01	2394.1	12.0	26	8.03	11.89	433.52	434.88	1815.52	208.79	2036.2	2321.4	3.41	5.17	8.06	11.05	402.73	403.99	1807.32	222.73	2041.1	2346.1	3.64	5.52
laboratory	Trichodesmium erythraeum	HCAO	1											5.243039399	0.768018722	32	27	245.0	1.0	7.79	0.01	2373.5	19.1	253.3	1.5	7.86	0.02	2350.9	55.4	26	7.65	32.75	1193.74	1197.48	2084.40	99.95	2217.1	2325.9	1.63	2.48	7.72	27.11	988.16	991.25	2027.27	114.21	2168.6	2303.8	1.86	2.83
laboratory	Trichodesmium erythraeum	ACLO	1											5.283947959	0.815704058	32	27	60.2	0.8	8.15	0.01	2365.5	19.3	89.2	1.6	8.21	0.01	2402.0	36.4	26	8.01	12.55	457.56	458.99	1830.70	201.14	2044.4	2318.1	3.28	4.98	8.07	10.78	392.86	394.09	1803.87	227.45	2042.1	2353.8	3.71	5.64
laboratory	Trichodesmium erythraeum	HCLO	1											6.812458026	0.987197791	32	27	61.9	1.0	7.81	0.01	2390.1	18.3	91.3	2.8	7.90	0.01	2409.2	17.0	26	7.67	31.35	1142.69	1146.27	2089.07	104.88	2225.3	2342.2	1.71	2.60	7.76	25.06	913.29	916.14	2054.79	126.95	2206.8	2360.9	2.07	3.15
laboratory	Trichodesmium erythraeum	ACAO	2											4.960271473	0.797999720	32	27	248.7	1.5	8.17	0.00	2368.9	32.8	256.7	1.5	8.20	0.01	2394.1	12.0	26	8.03	11.89	433.52	434.88	1815.52	208.79	2036.2	2321.4	3.41	5.17	8.06	11.05	402.73	403.99	1807.32	222.73	2041.1	2346.1	3.64	5.52
laboratory	Trichodesmium erythraeum	HCAO	2											5.196998499	0.733924983	32	27	245.0	1.0	7.79	0.01	2373.5	19.1	253.3	1.5	7.86	0.02	2350.9	55.4	26	7.65	32.75	1193.74	1197.48	2084.40	99.95	2217.1	2325.9	1.63	2.48	7.72	27.11	988.16	991.25	2027.27	114.21	2168.6	2303.8	1.86	2.83
laboratory	Trichodesmium erythraeum	ACLO	2											5.322078046	0.808816651	32	27	60.2	0.8	8.15	0.01	2365.5	19.3	89.2	1.6	8.21	0.01	2402.0	36.4	26	8.01	12.55	457.56	458.99	1830.70	201.14	2044.4	2318.1	3.28	4.98	8.07	10.78	392.86	394.09	1803.87	227.45	2042.1	2353.8	3.71	5.64
laboratory	Trichodesmium erythraeum	HCLO	2											6.374820615	0.960963138	32	27	61.9	1.0	7.81	0.01	2390.1	18.3	91.3	2.8	7.90	0.01	2409.2	17.0	26	7.67	31.35	1142.69	1146.27	2089.07	104.88	2225.3	2342.2	1.71	2.60	7.76	25.06	913.29	916.14	2054.79	126.95	2206.8	2360.9	2.07	3.15
laboratory	Trichodesmium erythraeum	ACAO	3											5.088294047	0.775147765	32	27	248.7	1.5	8.17	0.00	2368.9	32.8	256.7	1.5	8.20	0.01	2394.1	12.0	26	8.03	11.89	433.52	434.88	1815.52	208.79	2036.2	2321.4	3.41	5.17	8.06	11.05	402.73	403.99	1807.32	222.73	2041.1	2346.1	3.64	5.52
laboratory	Trichodesmium erythraeum	HCAO	3											4.905312688	0.717407929	32	27	245.0	1.0	7.79	0.01	2373.5	19.1	253.3	1.5	7.86	0.02	2350.9	55.4	26	7.65	32.75	1193.74	1197.48	2084.40	99.95	2217.1	2325.9	1.63	2.48	7.72	27.11	988.16	991.25	2027.27	114.21	2168.6	2303.8	1.86	2.83
laboratory	Trichodesmium erythraeum	ACLO	3											5.814993477	0.854448464	32	27	60.2	0.8	8.15	0.01	2365.5	19.3	89.2	1.6	8.21	0.01	2402.0	36.4	26	8.01	12.55	457.56	458.99	1830.70	201.14	2044.4	2318.1	3.28	4.98	8.07	10.78	392.86	394.09	1803.87	227.45	2042.1	2353.8	3.71	5.64
laboratory	Trichodesmium erythraeum	HCLO	3											7.727417802	1.088488182	32	27	61.9	1.0	7.81	0.01	2390.1	18.3	91.3	2.8	7.90	0.01	2409.2	17.0	26	7.67	31.35	1142.69	1146.27	2089.07	104.88	2225.3	2342.2	1.71	2.60	7.76	25.06	913.29	916.14	2054.79	126.95	2206.8	2360.9	2.07	3.15