Hörterer, Christina; Petereit, Jessica; Johansen, Johan; Buck, Bela Hieronymus (2023): Chemical analysis of diets, carcass and faeces of juvenile turbot (Scophthalmus maximus) fed with emerging protein sources [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.960982
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Abstract:
This study examined the growth response of juvenile turbot (Scophthalmus maximus) to diets with graded fishmeal (FM) replacement with plant, animal, and emerging protein sources (PLANT, PAP, and MIX) in comparison to a commercial-like diet (CTRL). The feeding experiment was carried out from April to July 2019 in the Centre for Aquaculture Research (ZAF) at the Alfred Wegener Institute for Polar and Marine research in Bremerhaven, Germany. The juvenile turbot (Scophthalmus maximus) were purchased from France Turbot (L'Épine, France) and acclimated to the recirculating aquaculture system (RAS) for 2 weeks prior to starting the 16 weeks experimental trial. To elucidate the effects of the protein sources and the level of FM replacement on the proximate and mineral composition of the carcass and the apparent digestibility of the diets, in this study. The chemical analysis of the diets was conducted in duplicates and of the carcass and faeces as pooled replicates per tank (n =5 tanks per diet). The carcass samples were minced frozen using a meat grinder (MADO Primus, Germany), refrozen at−20 °C and then freeze-dried for 48 h. The samples of the experimental diets and faeces were freeze-dried for 24 h. The experimental diets and whole body samples were further homogenised in a knife grinder (5000 rpm, 30 s, Grindomix GM 200, Retsch, Germany). The moisture content, ash, crude protein, crude lipid and energy of the experimental diets, carcass and faeces was determined after AOAC (1980). Moisture content of the feeds was determined by drying the samples at 105 °C for 24 h. The moisture content of the whole body and faeces was determined by freeze-drying. Total ash content was determined by combustion of the samples in a muffle oven at 550 °C for 6 h. The total nitrogen in the feed and whole body samples was determined following the automated Kjeldahl Method. Due to small sample volume in the faeces samples, the total nitrogen was determined after the Dumas method. For all samples, the measured total nitrogen was converted to equivalent crude protein (%) by the numerical factor of 6.25. Crude lipid was determined by acid hydrolysis. Gross energy was measured in an adiabatic bomb calorimeter (Model 6100; Parr Instrument, Germany). For the analysis of the mineral content, 0.2 g of freeze-dried and homogenised samples of the experimental diets, whole body and faeces was digested in 3 mL nitric acid (HNO3) (65%, trace grade) in a microwave oven (CEM MARS5, Germany) according to DIN EN 13,805 (2014). After digestion, the samples were diluted with Milli-Q water to 50 mL. Calcium, potassium, magnesium, phosphorus, arsenic, copper, iron, manganese, yttrium and zinc concentrations were analysed in an ICP-OES (iCAP7400; Fisher Scientific, Germany). As reference fish muscle (ERM – BB422, EU) was used.
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Related to:
Hörterer, Christina; Petereit, Jessica; Lannig, Gisela; Johansen, Johan; Pereira, Gabriella V; Conceição, Luis E C; Pastres, Roberto; Buck, Bela Hieronymus (2022): Sustainable fish feeds: potential of emerging protein sources in diets for juvenile turbot (Scophthalmus maximus) in RAS. Aquaculture International, 30, 1481-1504, https://doi.org/10.1007/s10499-022-00859-x
References:
Funding:
Parameter(s):
# | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
---|---|---|---|---|---|---|
1 | Experiment | Exp | Hörterer, Christina | GAIN (project)_SM1 (Species; Experiment 1) | ||
2 | Type of study | Study type | Hörterer, Christina | |||
3 | Species, unique identification | Species UID | Hörterer, Christina | |||
4 | Species, unique identification (Semantic URI) | Species UID (Semantic URI) | Hörterer, Christina | |||
5 | Species, unique identification (URI) | Species UID (URI) | Hörterer, Christina | |||
6 | Analysis | Analysis | Hörterer, Christina | |||
7 | Location | Location | Hörterer, Christina | |||
8 | Method comment | Method comm | Hörterer, Christina | System: Centre for Aquaculture Research (ZAF), Recirculating Aquaculture System (KL) #1 | ||
9 | Tank number | Tank No | Hörterer, Christina | Fibre glas tanks, 0.7 m3, total number 36 | ||
10 | Sample ID | Sample ID | Hörterer, Christina | |||
11 | Treatment | Treat | Hörterer, Christina | Formulation concept: CTRL - commercial-like diet; PLANT - plant protein sources, PAP - animal protein source, MIX - emerging protein source | ||
12 | Treatment | Treat | Hörterer, Christina | FMR - Fishmeal replacement level (0; 20; 40) | ||
13 | Material | Material | Hörterer, Christina | sampled material | ||
14 | Sampling date/time, experiment | Date/time sampling exp | Hörterer, Christina | Date fish was sampled from tank | ||
15 | Time point, descriptive | Time point | Hörterer, Christina | final sampling at end of experiment | ||
16 | Dry mass | Dry m | % | Hörterer, Christina | Drying according to AOAC (1980) | Dry matter, determined by drying the samples at 105 °C for 24 h after AOAC (1980) |
17 | Proteins | Protein | % | Hörterer, Christina | see comment | Crude protein, determined following Kjeldahl (diet and carcass) and Dumas (feces) Method after AOAC (1980) |
18 | Lipids | Lipids | % | Hörterer, Christina | Acid hydrolysis according to AOAC (1980) | Crude lipid, determined by acid hydrolysis after AOAC (1980) |
19 | Ash | Ash | % | Hörterer, Christina | Dry combustion | Crude ash, determined by combustion of the samples in a muffle oven at 550 °C for 6 h |
20 | Gross energy, per dry mass | GE/dm | MJ/kg | Hörterer, Christina | Compensated calorimeter, Parr, 6100 | measured in an adiabatic bomb calorimeter |
21 | Arsenic | As | mg/kg | Hörterer, Christina | Inductively coupled plasma–optical emission spectrometer (ICP-OES), Thermo Fisher Scientific Inc., iCAP 7400 | digestion according to DIN EN 13,805 (2014), concentrations were analysed in an ICP-OES on dry matter basis |
22 | Calcium | Ca | mg/kg | Hörterer, Christina | Inductively coupled plasma–optical emission spectrometer (ICP-OES), Thermo Fisher Scientific Inc., iCAP 7400 | digestion according to DIN EN 13,805 (2014), concentrations were analysed in an ICP-OES on dry matter basis |
23 | Copper | Cu | mg/kg | Hörterer, Christina | Inductively coupled plasma–optical emission spectrometer (ICP-OES), Thermo Fisher Scientific Inc., iCAP 7400 | digestion according to DIN EN 13,805 (2014), concentrations were analysed in an ICP-OES on dry matter basis |
24 | Iron | Fe | mg/kg | Hörterer, Christina | Inductively coupled plasma–optical emission spectrometer (ICP-OES), Thermo Fisher Scientific Inc., iCAP 7400 | digestion according to DIN EN 13,805 (2014), concentrations were analysed in an ICP-OES on dry matter basis |
25 | Potassium | K | mg/kg | Hörterer, Christina | Inductively coupled plasma–optical emission spectrometer (ICP-OES), Thermo Fisher Scientific Inc., iCAP 7400 | digestion according to DIN EN 13,805 (2014), concentrations were analysed in an ICP-OES on dry matter basis |
26 | Magnesium | Mg | mg/kg | Hörterer, Christina | Inductively coupled plasma–optical emission spectrometer (ICP-OES), Thermo Fisher Scientific Inc., iCAP 7400 | digestion according to DIN EN 13,805 (2014), concentrations were analysed in an ICP-OES on dry matter basis |
27 | Manganese | Mn | mg/kg | Hörterer, Christina | Inductively coupled plasma–optical emission spectrometer (ICP-OES), Thermo Fisher Scientific Inc., iCAP 7400 | digestion according to DIN EN 13,805 (2014), concentrations were analysed in an ICP-OES on dry matter basis |
28 | Sodium | Na | mg/kg | Hörterer, Christina | Inductively coupled plasma–optical emission spectrometer (ICP-OES), Thermo Fisher Scientific Inc., iCAP 7400 | digestion according to DIN EN 13,805 (2014), concentrations were analysed in an ICP-OES on dry matter basis |
29 | Phosphorus | P | mg/kg | Hörterer, Christina | Inductively coupled plasma–optical emission spectrometer (ICP-OES), Thermo Fisher Scientific Inc., iCAP 7400 | digestion according to DIN EN 13,805 (2014), concentrations were analysed in an ICP-OES on dry matter basis |
30 | Zinc | Zn | mg/kg | Hörterer, Christina | Inductively coupled plasma–optical emission spectrometer (ICP-OES), Thermo Fisher Scientific Inc., iCAP 7400 | digestion according to DIN EN 13,805 (2014), concentrations were analysed in an ICP-OES on dry matter basis |
31 | Yttrium | Y | mg/kg | Hörterer, Christina | Inductively coupled plasma–optical emission spectrometer (ICP-OES), Thermo Fisher Scientific Inc., iCAP 7400 | digestion according to DIN EN 13,805 (2014), concentrations were analysed in an ICP-OES on dry matter basis |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1167 data points