Hörterer, Christina; Lannig, Gisela; Buck, Bela Hieronymus (2024): Glycogen and glucose content of liver and muscle tissue of juvenile turbot (Scophthalmus maximus) fed with emerging protein sources [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.960625
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Abstract:
This study examined the growth response of juvenile turbot (Scophthalmus maximus) to diets with graded fishmeal (FM) replacement with plant, animal, and emerging protein sources (PLANT, PAP, and MIX) in comparison to a commercial-like diet (CTRL). The feeding experiment was carried out from April to July 2019 in the Centre for Aquaculture Research (ZAF) at the Alfred Wegener Institute for Polar and Marine research in Bremerhaven, Germany. The juvenile turbot (Scophthalmus maximus) were purchased from France Turbot (L'Épine, France) and acclimated to the recirculating aquaculture system (RAS) for 2 weeks prior to starting the 16 weeks experimental trial. To elucidate the effects of the protein sources and the level of FM replacement on the nutritional status of the fish, glycogen, free glucose and lipid content of liver and muscle tissue were determined at the end of the experiment (t4). Following the procedure described by Keppler and Decker (1988), glycogen content was determined photometrically after enzymatic hydrolysis of glycogen to glucose. Briefly, filet and liver samples (3 individual fish per tank; 15 fish per diet in total) were grinded under liquid nitrogen, and approx. 200 mg tissue was homogenised in 5×volume of icecold 0.6 M perchloric acid (PCA) (w:v). After one cycle of 20 s at 6000 rpm and 3 °C using Precellys 24 (Bertin Technologies, France), samples were sonicated for 2 min at 0 °C and 360 W (Branson Ultrasonics Sonifier 450; Fisher Scientific, Germany), and homogenates were immediately divided for the analysis of total and free glucose concentrations.
Related to:
Hörterer, Christina; Petereit, Jessica; Lannig, Gisela; Bock, Christian; Buck, Bela Hieronymus (2023): 1H-NMR-Based Metabolic Profiling in Muscle and Liver Tissue of Juvenile Turbot (Scophthalmus maximus) Fed with Plant and Animal Protein Sources. Metabolites, 13(5), 612, https://doi.org/10.3390/metabo13050612
Hörterer, Christina; Petereit, Jessica; Lannig, Gisela; Johansen, Johan; Pereira, Gabriella V; Conceição, Luis E C; Pastres, Roberto; Buck, Bela Hieronymus (2022): Sustainable fish feeds: potential of emerging protein sources in diets for juvenile turbot (Scophthalmus maximus) in RAS. Aquaculture International, 30, 1481-1504, https://doi.org/10.1007/s10499-022-00859-x
References:
Funding:
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Experiment | Exp | Hörterer, Christina | GAIN (project)_SM1 (Species; Experiment 1) | ||
| 2 | Type of study | Study type | Hörterer, Christina | |||
| 3 | Species, unique identification | Species UID | Hörterer, Christina | |||
| 4 | Species, unique identification (Semantic URI) | Species UID (Semantic URI) | Hörterer, Christina | |||
| 5 | Species, unique identification (URI) | Species UID (URI) | Hörterer, Christina | |||
| 6 | Location | Location | Hörterer, Christina | |||
| 7 | Method comment | Method comm | Hörterer, Christina | System: Centre for Aquaculture Research (ZAF), Recirculating Aquaculture System (KL) #1 | ||
| 8 | Sampling date/time, experiment | Date/time sampling exp | Hörterer, Christina | Date fish was sampled from tank | ||
| 9 | Time point, descriptive | Time point | Hörterer, Christina | final sampling at end of experiment | ||
| 10 | Tank number | Tank No | Hörterer, Christina | Fibre glas tanks, 0.7 m3, total number 36 | ||
| 11 | Identification | ID | Hörterer, Christina | Fish#: Individual Fish sampled | ||
| 12 | Sample ID | Sample ID | Hörterer, Christina | sampling point_Tank_Fish# | ||
| 13 | Treatment | Treat | Hörterer, Christina | Formulation concept: CTRL - commercial-like diet; PLANT - plant protein sources, PAP - animal protein source, MIX - emerging protein source | ||
| 14 | Treatment | Treat | Hörterer, Christina | FMR - Fishmeal replacement level (0; 20; 40) | ||
| 15 | Scophthalmus maximus, total length | S. maximus TL | cm | Hörterer, Christina | Fish measuring board | Body length measured from head to end of fin |
| 16 | Scophthalmus maximus, mass | S. maximus m | g | Hörterer, Christina | Laboratory balance, Sartorius, Cubis | Body weigth of fish with water dropped for 10 seconds |
| 17 | Scophthalmus maximus, liver mass | S. maximus liver m | g | Hörterer, Christina | Laboratory balance, Sartorius, Cubis | weight of the seperated liver |
| 18 | Scophthalmus maximus, liver glycogen, per wet mass | S. maximus liver gly wm | mg/g | Hörterer, Christina | Enzymatic analysis according to Keppler and Decker (1988) | concentration in the wet tissue |
| 19 | Scophthalmus maximus, liver glucose, per wet mass | S. maximus liver glc wm | mg/g | Hörterer, Christina | Enzymatic analysis according to Keppler and Decker (1988) | concentration in the wet tissue |
| 20 | Scophthalmus maximus, muscle glycogen, per wet mass | S. maximus muscle gly wm | mg/g | Hörterer, Christina | Enzymatic analysis according to Keppler and Decker (1988) | concentration in the wet tissue |
| 21 | Scophthalmus maximus, muscle glucose, per wet mass | S. maximus muscle glc wm | mg/g | Hörterer, Christina | Enzymatic analysis according to Keppler and Decker (1988) | concentration in the wet tissue |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1140 data points