Citation:

PANGAEA.960522

Name: Seawater carbonate chemistry and respiration rate of pteropod Limacina helicina
Published: 2023-07-10
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Laboratory experiment; Limacina helicina; Mollusca; Mortality/Survival; North Pacific; Pelagos; Respiration; Single species; Temperate; Zooplankton

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Abstract:

The pteropod Limacina helicina has become an important bioindicator species for the negative impacts of ocean acidification (OA) on marine ecosystems. However, pteropods diversified during earlier high CO2 periods in Earth history and currently inhabit regions that are naturally corrosive to their shells, suggesting that they possess mechanisms to survive unfavourable conditions. Recent work, which is still under considerable debate, has proposed that the periostracum, a thin organic coating on the outer shell, protects pteropods from shell dissolution. Here, we provide direct evidence that shows that damage to the L. helicina periostracum results in dissolution of the underlying shell when exposed to corrosive water for ∼8 d, while an intact periostracum protects the shell from dissolution under the same conditions. This important first line of defence suggests that pteropods are more resistant to OA-induced shell dissolution than is generally accepted.

Keyword(s):

Animalia; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Laboratory experiment; Limacina helicina; Mollusca; Mortality/Survival; North Pacific; Pelagos; Respiration; Single species; Temperate; Zooplankton

Supplement to:

Miller, M R; Oakes, R L; Covert, P A; Ianson, Debby; Dower, J F; Browman, Howard I (2023): Evidence for an effective defence against ocean acidification in the key bioindicator pteropod Limacina helicina. ICES Journal of Marine Science, 80(5), 1329-1341, https://doi.org/10.1093/icesjms/fsad059

Documentation:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2019): seacarb: Seawater Carbonate Chemistry. R package version 3.2.15. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 48.430000 * Longitude: -126.230000

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-07-10.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type of study	Study type		Miller, M R
2	Species, unique identification	Species UID		Miller, M R
3	Species, unique identification (URI)	Species UID (URI)		Miller, M R
4	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		Miller, M R
5	Identification	ID		Miller, M R		CRUISE
6	Sample code/label	Sample label		Miller, M R
7	Station label	Station		Miller, M R
8	Year of sampling	Year sampl		Miller, M R
9	Month	Month		Miller, M R
10	Day	Day		Miller, M R
11	LATITUDE	Latitude		Miller, M R		Geocode
12	LONGITUDE	Longitude		Miller, M R		Geocode
13	Hydrostatic pressure	Sigma H	kPa	Miller, M R	CTD
14	Temperature, water	Temp	°C	Miller, M R	CTD
15	Temperature, water	Temp	°C	Miller, M R		Incubation bottles were held at during the experiment
16	Salinity	Sal		Miller, M R	CTD
17	Density, sigma-theta (0)	Sigma-theta	kg/m³	Miller, M R
18	Oxygen, dissolved	DO	ml/l	Miller, M R
19	Oxygen	O2	µmol/kg	Miller, M R
20	Quality flag	QF		Miller, M R		DO
21	Oxygen	O2	µmol/kg	Miller, M R		adjusted for bottle headspace
22	Carbon, inorganic, dissolved	DIC	µmol/kg	Miller, M R
23	Quality flag	QF		Miller, M R		DIC
24	Alkalinity, total	AT	µmol/kg	Miller, M R
25	Quality flag	QF		Miller, M R		TA
26	Nitrate and Nitrite	[NO3]- + [NO2]-	µmol/l	Miller, M R
27	Quality flag	QF		Miller, M R		nitrate plus nitrite
28	Silicate	Si(OH)4	µmol/l	Miller, M R
29	Quality flag	QF		Miller, M R		Silicate
30	Phosphate	[PO4]3-	µmol/l	Miller, M R
31	Quality flag	QF		Miller, M R		Phosphate
32	Sample type	Samp type		Miller, M R
33	Treatment	Treat		Miller, M R
34	Individuals	Ind	#	Miller, M R		in incubation bottle
35	Individuals	Ind	#	Miller, M R		in incubation bottle that survived
36	Incubation duration	Inc dur	days	Miller, M R
37	Incubation duration	Inc dur	h	Miller, M R
38	pH	pH		Miller, M R		total scale
39	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Miller, M R
40	Aragonite saturation state	Omega Arg		Miller, M R
41	Calcite saturation state	Omega Cal		Miller, M R
42	Carbon, inorganic, dissolved, standard error	DIC std e	±	Miller, M R
43	Alkalinity, total, standard error	AT std e	±	Miller, M R
44	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error	pCO2water_SST_wet std e	±	Miller, M R
45	Aragonite saturation state, standard error	Omega Arg std e	±	Miller, M R
46	Calcite saturation state, standard error	Omega Cal std e	±	Miller, M R
47	Change	Change		Miller, M R		in dissolved inorganic carbon over incubation period (final-initial)
48	Change	Change		Miller, M R		dissolved oxygen, calculated from delta DIC and redfield respiration stoichiometry
49	Oxygen	O2	µmol/kg	Miller, M R		final dissolved oxygen (initial-delta oxygen)
50	Oxygen saturation	O2 sat	%	Miller, M R		final
51	Net oxygen consumption	N O2 con	µmol	Miller, M R		total
52	Net oxygen consumption	N O2 con	µmol	Miller, M R		per individual
53	Respiration rate, oxygen, per dry mass	Resp O2/dm	µmol/g/h	Miller, M R		calculated from dry body weight estimate of 1.4477766 g/ind (Moira Galbraith unpublished data)
54	Respiration rate, oxygen, per dry mass	Resp O2/dm	µmol/g/h	Miller, M R		calculated from dry body weight estimate of 2.0711 g/ind (Thibodeau et al. 2020)
55	Respiration rate, oxygen, per wet mass	Resp O2/wm	µmol/g/h	Miller, M R		calculated from wet body weight estimate of 6.42344852969905 g/ind (Comeau et al. 2010)
56	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
57	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
58	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
59	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
60	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
61	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
62	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
63	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
64	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1116 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Study type	2 Species UID	3 Species UID (URI)	4 Species UID (Semantic URI)	5 ID (CRUISE)	6 Sample label	7 Station	8 Year sampl	9 Month	10 Day	11 Latitude	12 Longitude	13 Sigma H [kPa] (CTD)	14 Temp [°C] (CTD)	15 Temp [°C] (Incubation bottles were held ...)	16 Sal (CTD)	17 Sigma-theta [kg/m³]	18 DO [ml/l]	19 O2 [µmol/kg]	20 QF (DO)	21 O2 [µmol/kg] (adjusted for bottle headspace)	22 DIC [µmol/kg]	23 QF (DIC)	24 AT [µmol/kg]	25 QF (TA)	26 [NO3]- + [NO2]- [µmol/l]	27 QF (nitrate plus nitrite)	28 Si(OH)4 [µmol/l]	29 QF (Silicate)	30 [PO4]3- [µmol/l]	31 QF (Phosphate)	32 Samp type	33 Treat	34 Ind [#] (in incubation bottle)	35 Ind [#] (in incubation bottle that sur...)	36 Inc dur [days]	37 Inc dur [h]	38 pH (total scale)	39 pCO2water_SST_wet [µatm]	40 Omega Arg	41 Omega Cal	42 DIC std e [±]	43 AT std e [±]	44 pCO2water_SST_wet std e [±]	45 Omega Arg std e [±]	46 Omega Cal std e [±]	47 Change (in dissolved inorganic carbon...)	48 Change (dissolved oxygen, calculated ...)	49 O2 [µmol/kg] (final dissolved oxygen (initi...)	50 O2 sat [%] (final)	51 N O2 con [µmol] (total)	52 N O2 con [µmol] (per individual)	53 Resp O2/dm [µmol/g/h] (calculated from dry body weig...)	54 Resp O2/dm [µmol/g/h] (calculated from dry body weig...)	55 Resp O2/wm [µmol/g/h] (calculated from wet body weig...)	56 CSC flag (Calculated using seacarb afte...)	57 pH (total scale, Calculated using...)	58 CO2 [µmol/kg] (Calculated using seacarb afte...)	59 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	60 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	61 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	62 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	63 Omega Arg (Calculated using seacarb afte...)	64 Omega Cal (Calculated using seacarb afte...)
laboratory	Limacina helicina	marinespecies.org	urn:lsid:marinespecies.org:taxname:140223	2019-023	M1	9	2019	9	1	48.43	-126.23	500	10.4877	8.955555556	32.3714	1025.067253	6.841	298.0	2	298.0000000	2006.76	2	2176.38	2	2.29	2	2.91	2	0.603	2	control end	supersaturated					8.077534366	351.8898534	1.882783818	2.980461195	6	5	16.88094759	0.072816365	0.018797735	4.250754908									15	8.08	16.17	350.51	351.89	1867.41	123.18	1.88	2.98
laboratory	Limacina helicina	marinespecies.org	urn:lsid:marinespecies.org:taxname:140223	2019-023	M2	9	2019	9	1	48.43	-126.23	500	10.4877	8.955555556	32.3714	1025.067253	6.841	298.0	2	298.0000000	1998.71	2	2165.28	2	2.29	2	2.91	2	0.603	2	control start	supersaturated					8.071385164	355.7422654	1.850251515	2.928962309	6	5	17.24319757	0.072501441	0.019016795	-3.798612987									15	8.07	16.35	354.36	355.75	1861.31	121.05	1.85	2.93
laboratory	Limacina helicina	marinespecies.org	urn:lsid:marinespecies.org:taxname:140223	2019-023	M5	9	2019	9	1	48.43	-126.23	500	10.4877	8.955555556	32.3714	1025.067253	6.841	298.0	2	298.0000000	2047.65	2	2169.14	2	2.29	2	2.91	2	0.603	2	incubation (3 individuals)	supersaturated	3	3	8.554166667	205.3000000	7.950782581	486.9859194	1.453470762	2.300855342	6	5	26.68441019	0.067782592	0.022010701	45.137832290	-65.15177679	232.8482232	81.33066265	33.38051284	11.126837610	37.43529395	26.16867490	8.437514886	15	7.95	22.38	485.08	486.98	1930.18	95.10	1.45	2.30
laboratory	Limacina helicina	marinespecies.org	urn:lsid:marinespecies.org:taxname:140223	2019-023	M6	9	2019	9	1	48.43	-126.23	500	10.4877	8.955555556	32.3714	1025.067253	6.841	298.0	2	298.0000000	2044.96	2	2160.86	2	2.29	2	2.91	2	0.603	2	incubation (4 individuals)	supersaturated	4	4	9.055555556	217.3333333	7.935474347	504.4082424	1.402993217	2.220949001	6	5	28.13344938	0.066980087	0.022463894	42.446977320	-61.26780688	236.7321931	82.68727960	31.39056086	7.847640214	24.94084653	17.43458741	5.621400066	15	7.94	23.18	502.47	504.44	1929.99	91.79	1.40	2.22
laboratory	Limacina helicina	marinespecies.org	urn:lsid:marinespecies.org:taxname:140223	2019-023	M7	9	2019	9	1	48.43	-126.23	500	10.4877	8.955555556	32.3714	1025.067253	6.841	298.0	2	298.0000000	2030.05	2	2169.93	2	2.29	2	2.91	2	0.603	2	incubation (3 individuals)	supersaturated	3	3	6.800694444	163.2166667	8.001761520	427.3719981	1.613072716	2.553506455	6	5	22.24251291	0.070005264	0.020709678	27.535929240	-39.74525635	258.2547436	90.20480871	20.36348209	6.787827364	28.72531046	20.08006968	6.474377762	15	8.00	19.64	425.71	427.38	1904.88	105.54	1.61	2.55
laboratory	Limacina helicina	marinespecies.org	urn:lsid:marinespecies.org:taxname:140223	2019-023	M8	9	2019	9	1	48.43	-126.23	500	10.4877	8.955555556	32.3714	1025.067253	6.841	298.0	2	298.0000000	2029.05	2	2168.77	2	2.29	2	2.91	2	0.603	2	incubation (3 individuals)	supersaturated	3	2	8.518750000	204.4500000	8.001466436	427.4646272	1.611231325	2.550591520	6	5	22.26301604	0.069982261	0.020725310	26.538457140	-38.30550889	259.6944911	90.70769258	19.62582748	6.541942493	22.10131194	15.44964620	4.981399338	15	8.00	19.64	425.81	427.48	1903.99	105.41	1.61	2.55
laboratory	Limacina helicina	marinespecies.org	urn:lsid:marinespecies.org:taxname:140223	2019-023	M9	9	2019	9	1	48.43	-126.23	500	10.4877	8.955555556	32.3714	1025.067253	6.841	298.0	2	298.0000000	2035.29	2	2167.91	2	2.29	2	2.91	2	0.603	2	incubation (3 individuals)	supersaturated	3	2	8.289583333	198.9500000	7.982180542	449.0653370	1.548800291	2.451762714	6	5	23.85952618	0.069172977	0.021220447	32.778457030	-47.31230119	250.6876988	87.56174465	24.24045751	8.080152504	28.05266085	19.60986237	6.322769734	15	7.98	20.63	447.32	449.07	1913.33	101.33	1.55	2.45
laboratory	Limacina helicina	marinespecies.org	urn:lsid:marinespecies.org:taxname:140223	2019-023	M10	9	2019	9	1	48.43	-126.23	500	10.4877	8.955555556	32.3714	1025.067253	6.841	298.0	2	298.0000000	2029.18	2	2166.64	2	2.29	2	2.91	2	0.603	2	incubation (3 individuals)	supersaturated	3	2	8.190972222	196.5833333	7.995547346	433.6066080	1.590433134	2.517667824	6	5	22.73721404	0.069720649	0.020890322	26.672999890	-38.49970738	259.5002926	90.63986173	19.72532508	6.575108359	23.10227061	16.14935387	5.207004717	15	8.00	19.92	431.90	433.59	1905.20	104.06	1.59	2.52
laboratory	Limacina helicina	marinespecies.org	urn:lsid:marinespecies.org:taxname:140223	2019-023	M12	9	2019	9	1	48.43	-126.23	500	10.4877	8.955555556	32.3714	1025.067253	6.841	298.0	2	298.0000000	2058.54	2	2165.50	2	2.29	2	2.91	2	0.603	2	incubation (4 individuals)	supersaturated	4	4	8.968750000	215.2500000	7.908615754	541.2178555	1.330234660	2.105771649	6	5	30.90733929	0.065668262	0.023106518	56.031155530	-80.87515845	217.1248416	75.83870299	41.43638743	10.359096860	33.24123730	23.23687196	7.492219373	15	7.91	24.87	539.10	541.21	1946.64	87.03	1.33	2.11
laboratory	Limacina helicina	marinespecies.org	urn:lsid:marinespecies.org:taxname:140223	2019-023	M16	9	2019	9	1	48.43	-126.23	2993	6.1726	8.955555556	33.9714	1026.319728	1.714	74.5	2	162.6260185	2258.70	2	2263.43	2	36.70	2	60.45	2	2.586	2	control end	undersaturated					7.542899605	1382.1240860	0.654652345	1.032832098	6	5	86.66539634	0.039091802	0.026572360	-5.454199334									15	7.54	62.92	1376.73	1382.13	2152.70	43.08	0.65	1.03
laboratory	Limacina helicina	marinespecies.org	urn:lsid:marinespecies.org:taxname:140223	2019-023	M17	9	2019	9	1	48.43	-126.23	2993	6.1726	8.955555556	33.9714	1026.319728	1.714	74.5	2	162.6260185	2261.75	2	2263.50	2	36.70	2	60.45	2	2.586	2	control start	undersaturated					7.532827351	1416.1565010	0.640369110	1.010297719	6	5	88.55634086	0.038217671	0.026527892	-2.405100598									15	7.53	64.47	1410.68	1416.21	2155.14	42.14	0.64	1.01
laboratory	Limacina helicina	marinespecies.org	urn:lsid:marinespecies.org:taxname:140223	2019-023	M20	9	2019	9	1	48.43	-126.23	2993	6.1726	8.955555556	33.9714	1026.319728	1.714	74.5	2	162.6260185	2298.68	2	2284.92	2	36.70	2	60.45	2	2.586	2	incubation (3 individuals)	undersaturated	3	3	8.543055556	205.0333333	7.481899523	1615.9550660	0.577952816	0.911824763	6	5	98.18071114	0.033878216	0.025911455	34.526991990	-49.83612995	112.7898886	39.85446466	25.58088550	8.526961835	28.72554611	20.08023440	6.474430874	15	7.48	73.57	1609.69	1616.00	2187.08	38.03	0.58	0.91
laboratory	Limacina helicina	marinespecies.org	urn:lsid:marinespecies.org:taxname:140223	2019-023	M21	9	2019	9	1	48.43	-126.23	2993	6.1726	8.955555556	33.9714	1026.319728	1.714	74.5	2	162.6260185	2302.62	2	2277.99	2	36.70	2	60.45	2	2.586	2	incubation (4 individuals)	undersaturated	4	4	9.036111111	216.8666667	7.445944482	1755.8999510	0.532172626	0.839598261	6	5	104.68421300	0.030834210	0.025516957	38.465797350	-55.52138674	107.1046318	37.84557124	28.49912782	7.124781954	22.69223229	15.86272170	5.114586465	15	7.45	79.94	1749.05	1755.92	2187.66	35.02	0.53	0.84
laboratory	Limacina helicina	marinespecies.org	urn:lsid:marinespecies.org:taxname:140223	2019-023	M24	9	2019	9	1	48.43	-126.23	2993	6.1726	8.955555556	33.9714	1026.319728	1.714	74.5	2	162.6260185	2330.18	2	2282.00	2	36.70	2	60.45	2	2.586	2	incubation (4 individuals)	undersaturated	4	0			7.372063733	2097.9232360	0.452460048	0.713837298	6	5	117.40293410	0.024969544	0.024124333	66.028167080									15	7.37	95.51	2089.77	2097.98	2204.90	29.77	0.45	0.71
laboratory	Limacina helicina	marinespecies.org	urn:lsid:marinespecies.org:taxname:140223	2019-023	M26	9	2019	9	1	48.43	-126.23	2993	6.1726	8.955555556	33.9714	1026.319728	1.714	74.5	2	162.6260185	2285.91	2	2279.97	2	36.70	2	60.45	2	2.586	2	incubation (3 individuals)	undersaturated	3	3	6.791666667	163.0000000	7.507605768	1515.8775590	0.610295124	0.962850584	6	5	93.38019433	0.036076571	0.026202478	21.755994860	-31.40252088	131.2234977	46.36800618	16.11891397	5.372971322	22.76802529	15.91570385	5.131669397	15	7.51	69.01	1509.99	1515.91	2176.74	40.16	0.61	0.96
laboratory	Limacina helicina	marinespecies.org	urn:lsid:marinespecies.org:taxname:140223	2019-023	M28	9	2019	9	1	48.43	-126.23	2993	6.1726	8.955555556	33.9714	1026.319728	1.714	74.5	2	162.6260185	2283.19	2	2281.30	2	36.70	2	60.45	2	2.586	2	incubation (3 individuals)	undersaturated	3	2	8.181944444	196.3666667	7.521202334	1467.9550960	0.629189934	0.992660554	6	5	90.84614412	0.037258117	0.026286644	19.040536100	-27.48303796	135.1429806	47.75296094	14.10704339	4.702347795	16.54037785	11.56234465	3.728024268	15	7.52	66.83	1462.25	1467.99	2174.96	41.40	0.63	0.99
laboratory	Limacina helicina	marinespecies.org	urn:lsid:marinespecies.org:taxname:140223	2019-023	M29	9	2019	9	1	48.43	-126.23	2993	6.1726	8.955555556	33.9714	1026.319728	1.714	74.5	2	162.6260185	2296.29	2	2288.43	2	36.70	2	60.45	2	2.586	2	incubation (3 individuals)	undersaturated	3	3	8.278472222	198.6833333	7.501585911	1543.7382070	0.604518595	0.953737069	6	5	94.55383962	0.035577520	0.026069580	32.135905450	-46.38484465	116.2411739	41.07398114	23.80934076	7.936446920	27.59072582	19.28695245	6.218654517	15	7.50	70.28	1537.74	1543.78	2186.23	39.78	0.60	0.95
laboratory	Limacina helicina	marinespecies.org	urn:lsid:marinespecies.org:taxname:140223	2019-023	M30	9	2019	9	1	48.43	-126.23	2993	6.1726	8.955555556	33.9714	1026.319728	1.714	74.5	2	162.6260185	2287.45	2	2284.71	2	36.70	2	60.45	2	2.586	2	incubation (3 individuals)	undersaturated	3	3	8.008333333	192.2000000	7.518449800	1479.9410550	0.626337369	0.988160118	6	5	91.38239598	0.037028263	0.026235378	23.303713670	-33.63649237	128.9895262	45.57862923	17.26561153	5.755203844	20.68263055	14.45793469	4.661643726	15	7.52	67.37	1474.06	1479.84	2178.86	41.22	0.63	0.99
laboratory	Limacina helicina	marinespecies.org	urn:lsid:marinespecies.org:taxname:140223	2019-023	163	9	2019	9	1	48.43	-126.23	2993	6.1726	8.955555556	33.9714	1026.319728	1.714	74.5	2	162.6260185	2264.15	6	2273.20	2	36.70	2	60.45	2	2.586	2	initial collection	undersaturated					7.557811696	1339.0640850	0.679343331	1.071786580	6	5	83.92810289	0.040417580	0.026518868										15	7.56	60.96	1333.86	1339.10	2158.49	44.70	0.68	1.07
laboratory	Limacina helicina	marinespecies.org	urn:lsid:marinespecies.org:taxname:140223	2019-023	173	9	2019	9	1	48.43	-126.23	500	10.4877	8.955555556	32.3714	1025.067253	6.841	298.0	2	298.0000000	2002.51	2	2162.99	2	2.29	2	2.91	2	0.603	2	initial collection	supersaturated					8.056375614	369.6059661	1.793969890	2.839868066	6	5	18.21557773	0.071973415	0.019396681										15	8.06	16.98	368.16	369.60	1868.15	117.37	1.79	2.84