Stief, Peter; Middelboe, Mathias (2023): Abundance of free-living microorganisms in the water surrounding sinking diatom aggregates incubated in rotating pressure and control tanks [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.960509, In: Stief, Peter; Schauberger, Clemens; Becker, Kevin W; Elvert, Marcus; Balmonte, John Paul; Franco-Cisterna, Belén; Middelboe, Mathias; Glud, Ronnie N (2023): Effects of increasing hydrostatic pressure on sinking diatom aggregates [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.960357
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Published: 2023-08-11 • DOI registered: 2023-09-09
Abstract:
The effect of increasing hydrostatic pressure on the microbial degradation, the organic matter composition, and the microbiome of 'marine snow' particles was studied in laboratory incubation experiments. Model aggregates were produced from the diatom Skeletonema marinoi and the natural microbial community of surface seawater collected in the Kattegat. The aggregates were incubated individually in rotating pressure and control tanks to keep them suspended during 20-day incubations in the dark and at 3°C. In the pressure tanks, hydrostatic pressure was increased at increments of 5 MPa per day to finally reach 100 MPa. This pressure scheme simulates the descent of diatom aggregates from the surface ocean down into a 10-km deep hadal trench. In the control tanks, pressure was always left at atmospheric level.
The abundance of free-living diatoms, bacteria, and viruses in the water surrounding sinking diatom aggregates was determined by microscopic (diatoms) and flow-cytometric cell counts (bacteria and viruses) in samples retrieved every 4 days throughout the 20-day incubation experiment.
Keyword(s):
Funding:
Danish National Research Foundation (DG), grant/award no. DNRF145: Danish Center for Hadal Research, HADAL
Coverage:
Latitude: 55.368167 * Longitude: 10.426563
Date/Time Start: 2021-07-27T13:48:26 * Date/Time End: 2022-11-02T08:27:14
Event(s):
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Experiment | Exp | Stief, Peter | |||
| 2 | Type of study | Study type | Stief, Peter | |||
| 3 | Date/time start, experiment | Date/time start exp | Stief, Peter | |||
| 4 | Date/time end, experiment | Date/time end exp | Stief, Peter | |||
| 5 | Experimental treatment | Exp treat | Stief, Peter | |||
| 6 | Replicate | Repl | Stief, Peter | |||
| 7 | Treatment: time after | T:time after | h | Stief, Peter | Time after starting the experimental incubation | |
| 8 | Treatment: pressure | T:pressure | MPa | Stief, Peter | Digital manometer, Keller AG, LEO5 | |
| 9 | Diatoms, free-living, abundance | Diatom abund FL | #/ml | Stief, Peter | Microscopic cell count, Fuchs-Rosenthal, Fuchs-Rosenthal counting chamber | |
| 10 | Bacteria, free-living, abundance | Bact abund FL | #/ml | Stief, Peter | Flow-cytometric cell count, BD Biosciences, BD FACS Canto™ II | |
| 11 | Bacterial, free-living, abundance, standard deviation | Bact abund FL std dev | ± | Stief, Peter | ||
| 12 | Virus, free-living, abundance | Virus abund FL | #/ml | Stief, Peter | Flow-cytometric cell count, BD Biosciences, BD FACS Canto™ II | |
| 13 | Virus, free-living, abundance, standard deviation | Virus abund FL std dev | ± | Stief, Peter | ||
| 14 | Laboratory | Lab | Stief, Peter |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC) * Processing Level: PANGAEA data processing level 3 (ProcLevel3)
Size:
168 data points
Data
| 1 Exp | 2 Study type | 3 Date/time start exp | 4 Date/time end exp | 5 Exp treat | 6 Repl | 7 T:time after [h] | 8 T:pressure [MPa] | 9 Diatom abund FL [#/ml] | 10 Bact abund FL [#/ml] | 11 Bact abund FL std dev [±] | 12 Virus abund FL [#/ml] | 13 Virus abund FL std dev [±] | 14 Lab |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ALT 3 | Laboratory experiment | 2022-10-12T14:22:14 | 2022-11-02T08:27:14 | Control | 3 | 0 | 0.1 | <2000 | 8.43E4 | 5.24E4 | 3.54E6 | 1.67E6 | HADAL Center, University of Southern Denmark |
| ALT 3 | Laboratory experiment | 2022-10-12T14:22:14 | 2022-11-02T08:27:14 | Control | 3 | 96 | 0.1 | <2000 | 6.68E4 | 4.04E4 | 3.53E6 | 7.13E5 | HADAL Center, University of Southern Denmark |
| ALT 3 | Laboratory experiment | 2022-10-12T14:22:14 | 2022-11-02T08:27:14 | Control | 3 | 192 | 0.1 | <2000 | 1.06E5 | 5.00E3 | 2.67E6 | 7.45E5 | HADAL Center, University of Southern Denmark |
| ALT 3 | Laboratory experiment | 2022-10-12T14:22:14 | 2022-11-02T08:27:14 | Control | 3 | 288 | 0.1 | <2000 | 1.31E5 | 4.93E4 | 3.10E6 | 2.38E5 | HADAL Center, University of Southern Denmark |
| ALT 3 | Laboratory experiment | 2022-10-12T14:22:14 | 2022-11-02T08:27:14 | Control | 3 | 384 | 0.1 | <2000 | 4.23E4 | 1.78E4 | 2.53E6 | 1.95E6 | HADAL Center, University of Southern Denmark |
| ALT 3 | Laboratory experiment | 2022-10-12T14:22:14 | 2022-11-02T08:27:14 | Control | 3 | 480 | 0.1 | <2000 | 1.08E5 | 1.86E4 | 4.19E6 | 1.51E6 | HADAL Center, University of Southern Denmark |
| ALT 3 | Laboratory experiment | 2022-10-12T14:22:14 | 2022-11-02T08:27:14 | Pressure | 3 | 0 | 0.1 | <2000 | 8.43E4 | 5.24E4 | 1.67E6 | 1.87E6 | HADAL Center, University of Southern Denmark |
| ALT 3 | Laboratory experiment | 2022-10-12T14:22:14 | 2022-11-02T08:27:14 | Pressure | 3 | 96 | 20.0 | <2000 | 9.13E4 | 4.86E4 | 1.73E6 | 4.23E6 | HADAL Center, University of Southern Denmark |
| ALT 3 | Laboratory experiment | 2022-10-12T14:22:14 | 2022-11-02T08:27:14 | Pressure | 3 | 192 | 40.0 | <2000 | 5.83E4 | 2.17E4 | 4.97E5 | 4.74E6 | HADAL Center, University of Southern Denmark |
| ALT 3 | Laboratory experiment | 2022-10-12T14:22:14 | 2022-11-02T08:27:14 | Pressure | 3 | 288 | 60.0 | <2000 | 7.06E4 | 5.32E4 | 9.67E5 | 2.89E6 | HADAL Center, University of Southern Denmark |
| ALT 3 | Laboratory experiment | 2022-10-12T14:22:14 | 2022-11-02T08:27:14 | Pressure | 3 | 384 | 80.0 | <2000 | 2.73E4 | 1.94E4 | 8.08E5 | 3.66E5 | HADAL Center, University of Southern Denmark |
| ALT 3 | Laboratory experiment | 2022-10-12T14:22:14 | 2022-11-02T08:27:14 | Pressure | 3 | 480 | 100.0 | <2000 | 4.10E4 | 1.90E4 | 6.60E5 | 4.57E6 | HADAL Center, University of Southern Denmark |