Becker, Kevin W; Elvert, Marcus (2023): Average unsaturation degree (AUD) of lipids in sinking diatom aggregates incubated in rotating pressure and control tanks [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.960507, In: Stief, Peter; Schauberger, Clemens; Becker, Kevin W; Elvert, Marcus; Balmonte, John Paul; Franco-Cisterna, Belén; Middelboe, Mathias; Glud, Ronnie N (2023): Effects of increasing hydrostatic pressure on sinking diatom aggregates [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.960357
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Published: 2023-08-11 • DOI registered: 2023-09-09
Abstract:
The effect of increasing hydrostatic pressure on the microbial degradation, the organic matter composition, and the microbiome of 'marine snow' particles was studied in laboratory incubation experiments. Model aggregates were produced from the diatom Skeletonema marinoi and the natural microbial community of surface seawater collected in the Kattegat. The aggregates were incubated individually in rotating pressure and control tanks to keep them suspended during 20-day incubations in the dark and at 3°C. In the pressure tanks, hydrostatic pressure was increased at increments of 5 MPa per day to finally reach 100 MPa. This pressure scheme simulates the descent of diatom aggregates from the surface ocean down into a 10-km deep hadal trench. In the control tanks, pressure was always left at atmospheric level.
Diatom-specific lipids in sinking diatom aggregates were analyzed by High Performance Liquid Chromatography (HPLC) coupled to quadrupole Time-of-Flight Mass Spectrometry (qToF-MS) in samples retrieved every 4 days throughout the 20-day incubation experiment. The AUD gives the average number of acyl double bonds in the fatty acids of 4 selected lipids.
Keyword(s):
Funding:
Danish National Research Foundation (DG), grant/award no. DNRF145: Danish Center for Hadal Research, HADAL
Coverage:
Latitude: 55.368167 * Longitude: 10.426563
Date/Time Start: 2021-07-27T13:48:26 * Date/Time End: 2022-11-02T08:27:14
Event(s):
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Experiment | Exp | Becker, Kevin W | |||
| 2 | Type of study | Study type | Becker, Kevin W | |||
| 3 | Date/time start, experiment | Date/time start exp | Becker, Kevin W | |||
| 4 | Date/time end, experiment | Date/time end exp | Becker, Kevin W | |||
| 5 | Experimental treatment | Exp treat | Becker, Kevin W | |||
| 6 | Replicate | Repl | Becker, Kevin W | |||
| 7 | Treatment: time after | T:time after | h | Becker, Kevin W | Time after starting the experimental incubation | |
| 8 | Treatment: pressure | T:pressure | MPa | Becker, Kevin W | Digital manometer, Keller AG, LEO5 | |
| 9 | Average unsaturation degree of triacylglycerol TAG-C48 | AUD TAG-C48 | Becker, Kevin W | Bruker maXis UHR TOF mass spectrometer coupled to a Dionex Ultimate 3000 UHPLC | ||
| 10 | Average unsaturation degree of triacylglycerol TAG-C48, standard deviation | AUD TAG-C48 std dev | Becker, Kevin W | |||
| 11 | Average unsaturation degree of triacylglycerol TAG-C50 | AUD TAG-C50 | Becker, Kevin W | Bruker maXis UHR TOF mass spectrometer coupled to a Dionex Ultimate 3000 UHPLC | ||
| 12 | Average unsaturation degree of triacylglycerol TAG-C50, standard deviation | AUD TAG-C50 std dev | Becker, Kevin W | |||
| 13 | Average unsaturation degree of monogalactosyl-diacylglycerol MGDG-C32 | AUD MGDG-C32 | Becker, Kevin W | Bruker maXis UHR TOF mass spectrometer coupled to a Dionex Ultimate 3000 UHPLC | ||
| 14 | Average unsaturation degree of monogalactosyl-diacylglycerol MGDG-C32, standard deviation | AUD MGDG-C32 std dev | Becker, Kevin W | |||
| 15 | Average unsaturation degree of monogalactosyl-diacylglycerol MGDG-C36 | AUD MGDG-C36 | Becker, Kevin W | Bruker maXis UHR TOF mass spectrometer coupled to a Dionex Ultimate 3000 UHPLC | ||
| 16 | Average unsaturation degree of monogalactosyl-diacylglycerol MGDG-C36, standard deviation | AUD MGDG-C36 std dev | Becker, Kevin W | |||
| 17 | Laboratory | Lab | Becker, Kevin W |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC) * Processing Level: PANGAEA data processing level 3 (ProcLevel3)
Size:
204 data points