Balmonte, John Paul; Franco-Cisterna, Belén (2023): Exoenzyme activities associated with sinking diatom aggregates incubated in rotating pressure and control tanks [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.960472, In: Stief, Peter; Schauberger, Clemens; Becker, Kevin W; Elvert, Marcus; Balmonte, John Paul; Franco-Cisterna, Belén; Middelboe, Mathias; Glud, Ronnie N (2023): Effects of increasing hydrostatic pressure on sinking diatom aggregates [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.960357
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Published: 2023-08-11 • DOI registered: 2023-09-09
Abstract:
The effect of increasing hydrostatic pressure on the microbial degradation, the organic matter composition, and the microbiome of 'marine snow' particles was studied in laboratory incubation experiments. Model aggregates were produced from the diatom Skeletonema marinoi and the natural microbial community of surface seawater collected in the Kattegat. The aggregates were incubated individually in rotating pressure and control tanks to keep them suspended during 20-day incubations in the dark and at 3°C. In the pressure tanks, hydrostatic pressure was increased at increments of 5 MPa per day to finally reach 100 MPa. This pressure scheme simulates the descent of diatom aggregates from the surface ocean down into a 10-km deep hadal trench. In the control tanks, pressure was always left at atmospheric level.
The activities of 5 exoenzymes (leucine-aminopeptidase, beta-glucosidase, laminarase, pullulanase, chondroitin sulfatase) associated with diatom aggregates were determined using fluorescently labeled substrates. Volumetric exoenzyme activities were calculated from the temporal change in fluorophore concentrations and the individual aggregate volume for samples retrieved every 4-8 days throughout the 20-day incubation experiment.
Keyword(s):
Funding:
Danish National Research Foundation (DG), grant/award no. DNRF145: Danish Center for Hadal Research, HADAL
Coverage:
Latitude: 55.368167 * Longitude: 10.426563
Date/Time Start: 2021-07-27T13:48:26 * Date/Time End: 2022-11-02T08:27:14
Event(s):
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Experiment | Exp | Balmonte, John Paul | |||
| 2 | Type of study | Study type | Balmonte, John Paul | |||
| 3 | Date/time start, experiment | Date/time start exp | Balmonte, John Paul | |||
| 4 | Date/time end, experiment | Date/time end exp | Balmonte, John Paul | |||
| 5 | Experimental treatment | Exp treat | Balmonte, John Paul | |||
| 6 | Replicate | Repl | Balmonte, John Paul | |||
| 7 | Treatment: time after | T:time after | h | Balmonte, John Paul | Time after starting the experimental incubation | |
| 8 | Treatment: pressure | T:pressure | MPa | Balmonte, John Paul | Digital manometer, Keller AG, LEO5 | |
| 9 | Leucin-aminopeptidase activity | Leucin-aminop. | µmol/mm3/day | Balmonte, John Paul | Fluorimeter, Promega, Quantifluor mini fluorimeter | Upon addition of leucine-7-amino-methylcoumarin (Leu-MCA) at 500 µmol/L |
| 10 | Leucin-aminopeptidase activity, standard deviation | Leucin-aminop. std dev | ± | Balmonte, John Paul | ||
| 11 | beta-glucosidase activity | B-glu | µmol/mm3/day | Balmonte, John Paul | Fluorimeter, Promega, Quantifluor mini fluorimeter | Upon addition of β-D-glucopyranoside-methylumbelliferyl (β-glu-MUF) at 500 µmol/L |
| 12 | beta-glucosidase activity, standard deviation | MUF-bglu std dev | ± | Balmonte, John Paul | ||
| 13 | Laminarase activity | Laminarase | µmol/mm3/day | Balmonte, John Paul | Gel permeation chromatography according to Arnosti 2003 | Upon addition of fluoresceine-labeled laminarin at 3.5 µmol/L |
| 14 | Laminarase activity, standard deviation | Laminarase std dev | ± | Balmonte, John Paul | ||
| 15 | Pullulanase activity | Pullulanase | µmol/mm3/day | Balmonte, John Paul | Gel permeation chromatography according to Arnosti 2003 | Upon addition of fluoresceine-labeled pullulan at 3.5 µmol/L |
| 16 | Pullulanase activity, standard deviation | Pullulanase std dev | ± | Balmonte, John Paul | ||
| 17 | Chondroitin sulfatase activity | Chondr sulf | µmol/mm3/day | Balmonte, John Paul | Gel permeation chromatography according to Arnosti 2003 | Upon addition of fluoresceine-labeled chondroitin sulfate at 3.5 µmol/L |
| 18 | Chondroitin sulfatase activity, standard deviation | Chondr sulf std dev | ± | Balmonte, John Paul | ||
| 19 | Laboratory | Lab | Balmonte, John Paul |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC) * Processing Level: PANGAEA data processing level 3 (ProcLevel3)
Size:
114 data points