Stief, Peter; Middelboe, Mathias (2023): Microbial cell abundance in sinking diatom aggregates incubated in rotating pressure and control tanks [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.960471, In: Stief, Peter; Schauberger, Clemens; Becker, Kevin W; Elvert, Marcus; Balmonte, John Paul; Franco-Cisterna, Belén; Middelboe, Mathias; Glud, Ronnie N (2023): Effects of increasing hydrostatic pressure on sinking diatom aggregates [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.960357
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Published: 2023-08-11 • DOI registered: 2023-09-09
Abstract:
The effect of increasing hydrostatic pressure on the microbial degradation, the organic matter composition, and the microbiome of 'marine snow' particles was studied in laboratory incubation experiments. Model aggregates were produced from the diatom Skeletonema marinoi and the natural microbial community of surface seawater collected in the Kattegat. The aggregates were incubated individually in rotating pressure and control tanks to keep them suspended during 20-day incubations in the dark and at 3°C. In the pressure tanks, hydrostatic pressure was increased at increments of 5 MPa per day to finally reach 100 MPa. This pressure scheme simulates the descent of diatom aggregates from the surface ocean down into a 10-km deep hadal trench. In the control tanks, pressure was always left at atmospheric level.
The abundance of diatoms, bacteria, and viruses in sinking diatom aggregates was determined by microscopic (diatoms) and flow-cytometric cell counts (bacteria and viruses) in samples retrieved every 4 days throughout the 20-day incubation experiment.
Keyword(s):
Funding:
Danish National Research Foundation (DG), grant/award no. DNRF145: Danish Center for Hadal Research, HADAL
Coverage:
Latitude: 55.368167 * Longitude: 10.426563
Date/Time Start: 2021-07-27T13:48:26 * Date/Time End: 2022-11-02T08:27:14
Event(s):
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Experiment | Exp | ||||
| 2 | Type of study | Study type | ||||
| 3 | Date/time start, experiment | Date/time start exp | ||||
| 4 | Date/time end, experiment | Date/time end exp | ||||
| 5 | Experimental treatment | Exp treat | ||||
| 6 | Replicate | Repl | ||||
| 7 | Treatment: time after | T:time after | h | Time after starting the experimental incubation | ||
| 8 | Treatment: pressure | T:pressure | MPa | Digital manometer, Keller AG, LEO5 | ||
| 9 | Diatom abundance | Diatom | #/mm3 | Microscopic cell count, Fuchs-Rosenthal, Fuchs-Rosenthal counting chamber | Abundance of intact diatom cells of dispersed aggregates is expressed per 1 mm**3 of intact aggregate | |
| 10 | Diatom abundance, standard deviation | Diatom std dev | ± | |||
| 11 | Bacterial abundance | Bact | #/mm3 | Flow-cytometric cell count, BD Biosciences, BD FACS Canto™ II | Abundance of bacteria of dispersed aggregates is expressed per 1 mm**3 of intact aggregate | |
| 12 | Bacterial abundance, standard deviation | Bact std dev | ± | |||
| 13 | Viral abundance | Virus | #/mm3 | Flow-cytometric cell count, BD Biosciences, BD FACS Canto™ II | Abundance of viruses of dispersed aggregates is expressed per 1 mm**3 of intact aggregate | |
| 14 | Viral abundance, standard deviation | Virus std dev | ± | |||
| 15 | Laboratory | Lab |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC) * Processing Level: PANGAEA data processing level 3 (ProcLevel3)
Size:
180 data points
Data
| 1 Exp | 2 Study type | 3 Date/time start exp | 4 Date/time end exp | 5 Exp treat | 6 Repl | 7 T:time after [h] | 8 T:pressure [MPa] | 9 Diatom [#/mm3] | 10 Diatom std dev [±] | 11 Bact [#/mm3] | 12 Bact std dev [±] | 13 Virus [#/mm3] | 14 Virus std dev [±] | 15 Lab |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ALT 2 | Laboratory experiment | 2021-10-21T15:29:10 | 2021-11-12T11:48:28 | Control | 3 | 0 | 0.1 | 2.08E5 | 1.05E4 | 1.52E5 | 7.81E4 | 2.43E5 | 1.06E5 | HADAL Center, University of Southern Denmark |
| ALT 2 | Laboratory experiment | 2021-10-21T15:29:10 | 2021-11-12T11:48:28 | Control | 3 | 96 | 0.1 | 1.77E5 | 3.86E4 | 5.23E5 | 3.24E5 | 4.65E5 | 1.80E5 | HADAL Center, University of Southern Denmark |
| ALT 2 | Laboratory experiment | 2021-10-21T15:29:10 | 2021-11-12T11:48:28 | Control | 3 | 192 | 0.1 | 1.63E5 | 7.91E4 | 1.55E6 | 1.20E6 | 2.96E6 | 9.56E5 | HADAL Center, University of Southern Denmark |
| ALT 2 | Laboratory experiment | 2021-10-21T15:29:10 | 2021-11-12T11:48:28 | Control | 3 | 288 | 0.1 | 1.31E5 | 4.82E4 | 9.95E5 | 1.96E5 | 3.88E6 | 1.26E6 | HADAL Center, University of Southern Denmark |
| ALT 2 | Laboratory experiment | 2021-10-21T15:29:10 | 2021-11-12T11:48:28 | Control | 3 | 384 | 0.1 | 1.25E5 | 2.06E4 | 1.37E6 | 4.97E5 | 2.97E6 | 8.30E5 | HADAL Center, University of Southern Denmark |
| ALT 2 | Laboratory experiment | 2021-10-21T15:29:10 | 2021-11-12T11:48:28 | Control | 3 | 480 | 0.1 | 1.28E5 | 3.43E4 | 2.52E6 | 1.31E6 | 4.76E6 | 1.26E6 | HADAL Center, University of Southern Denmark |
| ALT 2 | Laboratory experiment | 2021-10-21T15:29:10 | 2021-11-12T11:48:28 | Pressure | 3 | 0 | 0.1 | 2.08E5 | 1.05E4 | 1.52E5 | 7.81E4 | 2.43E5 | 1.06E5 | HADAL Center, University of Southern Denmark |
| ALT 2 | Laboratory experiment | 2021-10-21T15:29:10 | 2021-11-12T11:48:28 | Pressure | 3 | 96 | 20.0 | 2.05E5 | 6.02E4 | 3.56E5 | 1.80E5 | 8.12E5 | 5.84E5 | HADAL Center, University of Southern Denmark |
| ALT 2 | Laboratory experiment | 2021-10-21T15:29:10 | 2021-11-12T11:48:28 | Pressure | 3 | 192 | 40.0 | 1.81E5 | 6.64E4 | 5.51E5 | 1.70E5 | 2.09E6 | 8.16E5 | HADAL Center, University of Southern Denmark |
| ALT 2 | Laboratory experiment | 2021-10-21T15:29:10 | 2021-11-12T11:48:28 | Pressure | 3 | 288 | 60.0 | 2.21E5 | 4.83E4 | 3.52E5 | 7.92E4 | 8.59E5 | 1.90E5 | HADAL Center, University of Southern Denmark |
| ALT 2 | Laboratory experiment | 2021-10-21T15:29:10 | 2021-11-12T11:48:28 | Pressure | 3 | 384 | 80.0 | 2.11E5 | 4.14E4 | 3.31E5 | 6.94E4 | 1.19E6 | 3.13E5 | HADAL Center, University of Southern Denmark |
| ALT 2 | Laboratory experiment | 2021-10-21T15:29:10 | 2021-11-12T11:48:28 | Pressure | 3 | 480 | 100.0 | 1.87E5 | 9.23E3 | 4.30E5 | 1.63E5 | 2.05E6 | 7.00E5 | HADAL Center, University of Southern Denmark |