Durant, Andrea; Khodikian, Elissa; Porteus, Cosima S (2023): Seawater carbonate chemistry and antennular flicking and locomotory behaviours，olfactory nerve responses of Dungeness crab (Metacarcinus magister) [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.960337
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Abstract:
Crustacean olfaction is fundamental to most aspects of living and communicating in aquatic environments and more broadly, for individual- and population-level success. Accelerated ocean acidification from elevated CO2 threatens the ability of crabs to detect and respond to important olfactory-related cues. Here, we demonstrate that the ecologically and economically important Dungeness crab (Metacarcinus magister) exhibits reduced olfactory-related antennular flicking responses to a food cue when exposed to near-future CO2 levels, adding to the growing body of evidence of impaired crab behaviour. Underlying this altered behaviour, we find that crabs have lower olfactory nerve sensitivities (twofold reduction in antennular nerve activity) in response to a food cue when exposed to elevated CO2. This suggests that near-future CO2 levels will impact the threshold of detection of food by crabs. We also show that lower olfactory nerve sensitivity in elevated CO2 is accompanied by a decrease in the olfactory sensory neuron (OSN) expression of a principal chemosensory receptor protein, ionotropic receptor 25a (IR25a) which is fundamental for odorant coding and olfactory signalling cascades. The OSNs also exhibit morphological changes in the form of decreased surface areas of their somata. This study provides the first evidence of the effects of high CO2 levels at multiple levels of biological organization in marine crabs, linking physiological and cellular changes with whole animal behavioural responses.
Keyword(s):
Supplement to:
Durant, Andrea; Khodikian, Elissa; Porteus, Cosima S (2023): Ocean acidification alters foraging behaviour in Dungeness crab through impairment of the olfactory pathway. Global Change Biology, 29(14), 4126-4139, https://doi.org/10.1111/gcb.16738
Documentation:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2022): seacarb: seawater carbonate chemistry with R. R package version 3.3.1. https://cran.r-project.org/web/packages/seacarb/index.html
Project(s):
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-07-05.
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Type of study | Study type | Durant, Andrea | |||
| 2 | Species, unique identification | Species UID | Durant, Andrea | |||
| 3 | Species, unique identification (URI) | Species UID (URI) | Durant, Andrea | |||
| 4 | Species, unique identification (Semantic URI) | Species UID (Semantic URI) | Durant, Andrea | |||
| 5 | Experiment | Exp | Durant, Andrea | |||
| 6 | Figure | Fig | Durant, Andrea | |||
| 7 | Sampling date/time, experiment | Date/time sampling exp | Durant, Andrea | |||
| 8 | Treatment | Treat | Durant, Andrea | |||
| 9 | Identification | ID | Durant, Andrea | Crab | ||
| 10 | Experiment duration | Exp duration | days | Durant, Andrea | Time of exposure | |
| 11 | Antennular flicking rate | Antennular flick | #/min | Durant, Andrea | from 0 - 1 minutes (no odorant) | |
| 12 | Antennular flicking rate | Antennular flick | #/min | Durant, Andrea | from 2 - 3 minutes (no odorant) | |
| 13 | Antennular flicking rate | Antennular flick | #/min | Durant, Andrea | from 4 - 5 minutes (no odorant) | |
| 14 | Antennular flicking rate | Antennular flick | #/min | Durant, Andrea | from 0 - 1 minutes (10^-6M cad) | |
| 15 | Antennular flicking rate | Antennular flick | #/min | Durant, Andrea | from 2 - 3 minutes (10^-6M cad) | |
| 16 | Antennular flicking rate | Antennular flick | #/min | Durant, Andrea | from 4 - 5 minutes (10^-6M cad) | |
| 17 | Antennular flicking rate | Antennular flick | #/min | Durant, Andrea | from 0 - 1 minutes (10^-5M cad) | |
| 18 | Antennular flicking rate | Antennular flick | #/min | Durant, Andrea | from 2 - 3 minutes (10^-5M cad) | |
| 19 | Antennular flicking rate | Antennular flick | #/min | Durant, Andrea | from 4 - 5 minutes (10^-5M cad) | |
| 20 | Antennular flicking rate | Antennular flick | #/min | Durant, Andrea | from 0 - 1 minutes (10^-4M cad) | |
| 21 | Antennular flicking rate | Antennular flick | #/min | Durant, Andrea | from 2 - 3 minutes (10^-4M cad) | |
| 22 | Antennular flicking rate | Antennular flick | #/min | Durant, Andrea | from 4 - 5 minutes (10^-4M cad) | |
| 23 | Antennular flicking rate | Antennular flick | #/min | Durant, Andrea | from 4 - 5 minutes (10^-4M cad) | |
| 24 | Antennular flicking rate | Antennular flick | #/min | Durant, Andrea | from 2 - 3 minutes (10^-3M cad) | |
| 25 | Antennular flicking rate | Antennular flick | #/min | Durant, Andrea | from 4 - 5 minutes (10^-3M cad) | |
| 26 | Antennular flicking rate | Antennular flick | #/min | Durant, Andrea | from 0 - 1 (10^-3M NH4Cl) | |
| 27 | Antennular flicking rate | Antennular flick | #/min | Durant, Andrea | from 2 - 3 (10^-3M NH4Cl) | |
| 28 | Antennular flicking rate | Antennular flick | #/min | Durant, Andrea | from 4 - 5 (10^-3M NH4Cl) | |
| 29 | Salinity | Sal | Durant, Andrea | in behaviour tank | ||
| 30 | Temperature, water | Temp | °C | Durant, Andrea | in behaviour tank | |
| 31 | pH | pH | Durant, Andrea | NBS scale, in behaviour tank | ||
| 32 | Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) | pCO2water_SST_wet | µatm | Durant, Andrea | in behaviour tank | |
| 33 | Fugacity of carbon dioxide (water) at sea surface temperature (wet air) | fCO2water_SST_wet | µatm | Durant, Andrea | in behaviour tank | |
| 34 | Bicarbonate ion | [HCO3]- | µmol/kg | Durant, Andrea | in behaviour tank | |
| 35 | Carbonate ion | [CO3]2- | µmol/kg | Durant, Andrea | in behaviour tank | |
| 36 | Carbon, inorganic, dissolved | DIC | µmol/kg | Durant, Andrea | in behaviour tank | |
| 37 | Alkalinity, total | AT | µmol/kg | Durant, Andrea | in behaviour tank | |
| 38 | Aragonite saturation state | Omega Arg | Durant, Andrea | in behaviour tank | ||
| 39 | Calcite saturation state | Omega Cal | Durant, Andrea | in behaviour tank | ||
| 40 | Salinity | Sal | Durant, Andrea | in exposure | ||
| 41 | Salinity, standard deviation | Sal std dev | ± | Durant, Andrea | in exposure | |
| 42 | Temperature, water | Temp | °C | Durant, Andrea | in exposure | |
| 43 | Temperature, water, standard deviation | Temp std dev | ± | Durant, Andrea | in exposure | |
| 44 | pH | pH | Durant, Andrea | in exposure, NBS scale | ||
| 45 | pH, standard deviation | pH std dev | ± | Durant, Andrea | in exposure, NBS scale | |
| 46 | Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) | pCO2water_SST_wet | µatm | Durant, Andrea | in exposure | |
| 47 | Partial pressure of carbon dioxide, standard deviation | pCO2 std dev | ± | Durant, Andrea | in exposure | |
| 48 | Fugacity of carbon dioxide (water) at sea surface temperature (wet air) | fCO2water_SST_wet | µatm | Durant, Andrea | in exposure | |
| 49 | Bicarbonate ion | [HCO3]- | µmol/kg | Durant, Andrea | in exposure | |
| 50 | Carbonate ion | [CO3]2- | µmol/kg | Durant, Andrea | in exposure | |
| 51 | Carbon, inorganic, dissolved | DIC | µmol/kg | Durant, Andrea | in exposure | |
| 52 | Alkalinity, total | AT | µmol/kg | Durant, Andrea | in exposure | |
| 53 | Aragonite saturation state | Omega Arg | Durant, Andrea | in exposure | ||
| 54 | Calcite saturation state | Omega Cal | Durant, Andrea | in exposure | ||
| 55 | Total distance travelled | Tot dist trav | pixel | Durant, Andrea | from 0 - 1 minutes (no odorant) | |
| 56 | Total distance travelled | Tot dist trav | pixel | Durant, Andrea | from 2 - 3 minutes (no odorant) | |
| 57 | Total distance travelled | Tot dist trav | pixel | Durant, Andrea | from 4 - 5 minutes (no odorant) | |
| 58 | Total distance travelled | Tot dist trav | pixel | Durant, Andrea | from 0 - 1 minutes (10^-6M cad) | |
| 59 | Total distance travelled | Tot dist trav | pixel | Durant, Andrea | from 2 - 3 minutes (10^-6M cad) | |
| 60 | Total distance travelled | Tot dist trav | pixel | Durant, Andrea | from 4 - 5 minutes (10^-6M cad) | |
| 61 | Total distance travelled | Tot dist trav | pixel | Durant, Andrea | from 0 - 1 minutes (10^-5M cad) | |
| 62 | Total distance travelled | Tot dist trav | pixel | Durant, Andrea | from 2 - 3 minutes (10^-5M cad) | |
| 63 | Total distance travelled | Tot dist trav | pixel | Durant, Andrea | from 4 - 5 minutes (10^-5M cad) | |
| 64 | Total distance travelled | Tot dist trav | pixel | Durant, Andrea | from 0 - 1 minutes (10^-4M cad) | |
| 65 | Total distance travelled | Tot dist trav | pixel | Durant, Andrea | from 2 - 3 minutes (10^-4M cad) | |
| 66 | Total distance travelled | Tot dist trav | pixel | Durant, Andrea | from 4 - 5 minutes (10^-4M cad) | |
| 67 | Total distance travelled | Tot dist trav | pixel | Durant, Andrea | from 0 - 1 minutes (10^-3M cad) | |
| 68 | Total distance travelled | Tot dist trav | pixel | Durant, Andrea | from 2 - 3 minutes (10^-3M cad) | |
| 69 | Total distance travelled | Tot dist trav | pixel | Durant, Andrea | from 4 - 5 minutes (10^-3M cad) | |
| 70 | Total distance travelled | Tot dist trav | pixel | Durant, Andrea | from 0 - 1 (10^-3M NH4Cl) | |
| 71 | Total distance travelled | Tot dist trav | pixel | Durant, Andrea | from 2 - 3 (10^-3M NH4Cl) | |
| 72 | Total distance travelled | Tot dist trav | pixel | Durant, Andrea | from 4 - 5 (10^-3M NH4Cl) | |
| 73 | Cadaverine | Cad | mol/l | Durant, Andrea | ||
| 74 | Replicate | Repl | Durant, Andrea | |||
| 75 | Integrated nerve response | Int nerve resp | mV | Durant, Andrea | ||
| 76 | Ammonium chloride | NH4Cl | mol/l | Durant, Andrea | ||
| 77 | Total cell fluorescence, corrected | CTCF | arbitrary units | Durant, Andrea | ||
| 78 | Area | Area | arbitrary units | Durant, Andrea | OSN Cell Body | |
| 79 | mRNA abundance, relative | mRNA abund rel | Durant, Andrea | NKA | ||
| 80 | mRNA abundance, relative | mRNA abund rel | Durant, Andrea | VA | ||
| 81 | mRNA abundance, relative | mRNA abund rel | Durant, Andrea | Rh | ||
| 82 | Carbonate system computation flag | CSC flag | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | ||
| 83 | pH | pH | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | in exposure, total scale | |
| 84 | Carbon dioxide | CO2 | µmol/kg | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | in exposure |
| 85 | Fugacity of carbon dioxide (water) at sea surface temperature (wet air) | fCO2water_SST_wet | µatm | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | in exposure |
| 86 | Bicarbonate ion | [HCO3]- | µmol/kg | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | in exposure |
| 87 | Carbonate ion | [CO3]2- | µmol/kg | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | in exposure |
| 88 | Carbon, inorganic, dissolved | DIC | µmol/kg | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | in exposure |
| 89 | Alkalinity, total | AT | µmol/kg | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | in exposure |
| 90 | Aragonite saturation state | Omega Arg | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | in exposure | |
| 91 | Calcite saturation state | Omega Cal | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | in exposure |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
18008 data points
Download Data
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