Citation:

PANGAEA.960043

Name: Seawater carbonate chemistry and biomineralization pathways and shell material properties of Magallana gigas and Mytilus spp.
Published: 2023-06-26
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Growth/Morphology; Laboratory experiment; Laboratory strains; Magallana gigas; Mytilus spp.; Not applicable; Other; Other studied parameter or process; Single species; Temperature

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Abstract:

Molluscs are among the organisms affected by ocean acidification (OA), relying on carbon for shell biomineralization. Metabolic and environmental sourcing are two pathways potentially affected by OA, but the circumstances and patterns by which they are altered are poorly understood. From previous studies, mollusc shells grown under OA appear smaller in size, brittle and thinner, suggesting an important alteration in carbon sequestration. However, supplementary feeding experiments have shown promising results in offsetting the negative consequences of OA on shell growth. Our study compared carbon uptake by δ13C tracing and deposition into mantle tissue and shell layers in Magallana gigas and Mytilus species, two economically valuable and common species. After subjecting the species to 7.7 pH, +2 °C seawater, and enhanced feeding, both species maintain shell growth and metabolic pathways under OA without benefitting from extra feeding, thus, showing effective acclimation to rapid and short-term environmental change. Mytilus spp. increases metabolic carbon into the calcite and environmental sourcing of carbon into the shell aragonite in low pH and high temperature conditions. Low pH affects M. gigas mantle nitrogen isotopes maintaining growth. Calcite biomineralization pathway differs between the two species and suggests species-specific response to OA.

Keyword(s):

Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Growth/Morphology; Laboratory experiment; Laboratory strains; Magallana gigas; Mytilus spp.; Not applicable; Other; Other studied parameter or process; Single species; Temperature

Supplement to:

Mele, Isabella; McGill, Rona A R; Thompson, J; Fennell, James; Fitzer, Susan C (2023): Ocean acidification, warming and feeding impacts on biomineralization pathways and shell material properties of Magallana gigas and Mytilus spp. Marine Environmental Research, 186, 105925, https://doi.org/10.1016/j.marenvres.2023.105925

Documentation:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2022): seacarb: seawater carbonate chemistry with R. R package version 3.3.1. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-06-26.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type of study	Study type		Fitzer, Susan C
2	Species	Species		Fitzer, Susan C
3	Treatment	Treat		Fitzer, Susan C
4	Mass	Mass	kg	Fitzer, Susan C		shell
5	Shell length	Shell l	mm	Fitzer, Susan C
6	Shell width	Shell w	mm	Fitzer, Susan C
7	Shell thickness	Shell thick	mm	Fitzer, Susan C
8	Shell thickness index	STI		Fitzer, Susan C
9	Diagonal length	Diag l	mm	Fitzer, Susan C		1
10	Diagonal length	Diag l	mm	Fitzer, Susan C		2
11	Vickers Hardness	VH	HV	Fitzer, Susan C
12	Vickers hardness	VH	MPa	Fitzer, Susan C
13	Treatment: pH	T:pH		Fitzer, Susan C
14	Treatment: temperature	T:temp	°C	Fitzer, Susan C
15	Treatment	Treat		Fitzer, Susan C		Feeding
16	Identification	ID		Fitzer, Susan C		Tank
17	δ13C	δ13C	‰ PDB	Fitzer, Susan C		mantle
18	δ15N	δ15N	‰	Fitzer, Susan C		mantle
19	δ13C, calcite	δ13C cal	‰ PDB	Fitzer, Susan C
20	δ18O, calcite	δ18O cal	‰ PDB	Fitzer, Susan C
21	δ13C, aragonite	δ13C arg	‰ PDB	Fitzer, Susan C
22	δ18O, aragonite	δ18O arg	‰ PDB	Fitzer, Susan C
23	Cell density	Cells	#/ml	Fitzer, Susan C		Feeding
24	Temperature, water	Temp	°C	Fitzer, Susan C
25	Temperature, water, standard deviation	Temp std dev	±	Fitzer, Susan C
26	pH	pH		Fitzer, Susan C		NBS scale
27	pH, standard deviation	pH std dev	±	Fitzer, Susan C		NBS scale
28	Salinity	Sal		Fitzer, Susan C
29	Salinity, standard deviation	Sal std dev	±	Fitzer, Susan C
30	δ13C, dissolved inorganic carbon	δ13C DIC	‰ PDB	Fitzer, Susan C		in DIC
31	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Fitzer, Susan C
32	Replicate	Repl		Fitzer, Susan C
33	Carbon, inorganic, dissolved	DIC	µmol/kg	Fitzer, Susan C
34	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
36	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
40	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

5968 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Study type	2 Species	3 Treat	4 Mass [kg] (shell)	5 Shell l [mm]	6 Shell w [mm]	7 Shell thick [mm]	8 STI	9 Diag l [mm] (1)	10 Diag l [mm] (2)	11 VH [HV]	12 VH [MPa]	13 T:pH	14 T:temp [°C]	15 Treat (Feeding)	16 ID (Tank)	17 δ13C [‰ PDB] (mantle)	18 δ15N [‰] (mantle)	19 δ13C cal [‰ PDB]	20 δ18O cal [‰ PDB]	21 δ13C arg [‰ PDB]	22 δ18O arg [‰ PDB]	23 Cells [#/ml] (Feeding)	24 Temp [°C]	25 Temp std dev [±]	26 pH (NBS scale)	27 pH std dev [±] (NBS scale)	28 Sal	29 Sal std dev [±]	30 δ13C DIC [‰ PDB] (in DIC)	31 pCO2water_SST_wet [µatm]	32 Repl	33 DIC [µmol/kg]	34 CSC flag	35 pH (total scale)	36 CO2 [µmol/kg]	37 fCO2water_SST_wet [µatm]	38 pCO2water_SST_wet [µatm]	39 [HCO3]- [µmol/kg]	40 [CO3]2- [µmol/kg]	41 AT [µmol/kg]	42 Omega Arg	43 Omega Cal
laboratory	Magallana gigas	1	0.059	69.0	56.0	36.4	0.815022	73.18400000	73.1840	103.84813430	1018.4023060	8.1	14	control								8000000	11.45	0.86	7.91	0.08	28.42	4.07	-1.49	506	1-4	3096	27	7.79	48.08	1106.35	1110.55	2947.21	100.71	3179.80	1.56	2.49
laboratory	Magallana gigas	1	0.053	105.0	51.8	17.9	0.586330	77.43900000	77.4680	92.71476557	909.2212558	8.1	14	control								8000000	11.45	0.86	7.91	0.08	28.42	4.07	-1.49	506	1-4	3096	27	7.79	48.08	1106.35	1110.55	2947.21	100.71	3179.80	1.56	2.49
laboratory	Magallana gigas	1	0.047	72.3	56.6	19.2	0.692424	72.09200000	72.1160	106.98238620	1049.1388180	8.1	14	control								8000000	11.45	0.86	7.91	0.08	28.42	4.07	-1.49	506	1-4	3096	27	7.79	48.08	1106.35	1110.55	2947.21	100.71	3179.80	1.56	2.49
laboratory	Magallana gigas	1	0.061	81.3	49.5	27.0	0.847144	72.23500000	73.9720	104.09166290	1020.7905060	8.1	14	control								8000000	11.45	0.86	7.91	0.08	28.42	4.07	-1.49	506	1-4	3096	27	7.79	48.08	1106.35	1110.55	2947.21	100.71	3179.80	1.56	2.49
laboratory	Magallana gigas	2	0.059	90.2	58.7	21.3	0.666850	75.95700000	75.9570	96.40407181	945.4009908	7.7	14	control								8000000	11.70	0.84	7.71	0.07	32.78	1.42	-5.49	826	5-8	1214	27	7.60	28.00	666.03	668.55	1157.45	28.55	1239.80	0.44	0.69
laboratory	Magallana gigas	2	0.044	81.0	42.2	16.5	0.763210	66.62000000	67.9370	122.89097530	1205.1487830	7.7	14	control								8000000	11.70	0.84	7.71	0.07	32.78	1.42	-5.49	826	5-8	1214	27	7.60	28.00	666.03	668.55	1157.45	28.55	1239.80	0.44	0.69
laboratory	Magallana gigas	2	0.057	86.2	43.0	24.4	0.851462	75.46700000	75.4930	97.62638641	957.3878023	7.7	14	control								8000000	11.70	0.84	7.71	0.07	32.78	1.42	-5.49	826	5-8	1214	27	7.60	28.00	666.03	668.55	1157.45	28.55	1239.80	0.44	0.69
laboratory	Magallana gigas	3	0.052	87.5	47.1	25.7	0.705119	76.91777778	77.8480	92.88739778	910.9141994	8.1	14	Extra Feeding								16000000	11.55	0.87	7.95	0.15	28.42	4.07	-1.76	661	9-12	3920	27	7.83	55.28	1276.18	1281.02	3724.63	140.09	4038.80	2.17	3.47
laboratory	Magallana gigas	3	0.045	80.2	49.5	27.7	0.629733	82.45700000	81.1820	83.08903367	814.8250720	8.1	14	Extra Feeding								16000000	11.55	0.87	7.95	0.15	28.42	4.07	-1.76	661	9-12	3920	27	7.83	55.28	1276.18	1281.02	3724.63	140.09	4038.80	2.17	3.47
laboratory	Magallana gigas	3	0.061	78.5	55.3	30.2	0.785124	71.70300000	71.6810	108.21553290	1061.2318560	8.1	14	Extra Feeding								16000000	11.55	0.87	7.95	0.15	28.42	4.07	-1.76	661	9-12	3920	27	7.83	55.28	1276.18	1281.02	3724.63	140.09	4038.80	2.17	3.47
laboratory	Magallana gigas	4	0.056	84.2	51.1	26.0	0.738487	75.39800000	76.0280	97.02811461	951.5207601	7.7	14	Extra Feeding								16000000	11.80	0.85	7.67	0.13	36.10	3.84	-6.56	951	13-16	1461	27	7.56	35.35	859.46	862.70	1391.27	34.38	1486.90	0.52	0.81
laboratory	Magallana gigas	4	0.045	89.2	41.5	23.2	0.675502	76.30700000	76.0040	95.90255013	940.4827432	7.7	14	Extra Feeding								16000000	11.80	0.85	7.67	0.13	36.10	3.84	-6.56	951	13-16	1461	27	7.56	35.35	859.46	862.70	1391.27	34.38	1486.90	0.52	0.81
laboratory	Magallana gigas	5	0.034	68.0	43.4	17.5	0.680217	77.05200000	77.0460	93.69080538	918.7929366	8.1	16	control								8000000	13.65	1.03	7.96	0.09	36.58	3.84	-1.57	521	17-20	3490	27	7.85	40.94	1058.06	1061.96	3278.56	170.50	3672.40	2.58	4.02
laboratory	Magallana gigas	5	0.053	82.6	52.3	25.2	0.703623	78.63500000	78.7440	89.82508179	880.8831383	8.1	16	control								8000000	13.65	1.03	7.96	0.09	36.58	3.84	-1.57	521	17-20	3490	27	7.85	40.94	1058.06	1061.96	3278.56	170.50	3672.40	2.58	4.02
laboratory	Magallana gigas	6	0.040	69.9	56.9	19.8	0.604687	78.84000000	79.3300	88.92971789	872.1026179	7.7	16	control								8000000	13.60	1.03	7.72	0.93	36.10	3.84	-2.67	800	21-24	1281	27	7.61	26.65	685.69	688.22	1218.46	35.90	1321.50	0.54	0.85
laboratory	Magallana gigas	6	0.057	77.0	59.0	29.8	0.712971	76.45300000	76.4590	95.14979111	933.1006990	7.7	16	control								8000000	13.60	1.03	7.72	0.93	36.10	3.84	-2.67	800	21-24	1281	27	7.61	26.65	685.69	688.22	1218.46	35.90	1321.50	0.54	0.85
laboratory	Magallana gigas	6	0.057	96.7	49.1	24.7	0.682748	72.32800000	72.5370	106.01441920	1039.6463040	7.7	16	control								8000000	13.60	1.03	7.72	0.93	36.10	3.84	-2.67	800	21-24	1281	27	7.61	26.65	685.69	688.22	1218.46	35.90	1321.50	0.54	0.85
laboratory	Magallana gigas	6	0.060	81.6	50.0	29.4	0.807031	73.50300000	73.7830	102.55801580	1005.7505660	7.7	16	control								8000000	13.60	1.03	7.72	0.93	36.10	3.84	-2.67	800	21-24	1281	27	7.61	26.65	685.69	688.22	1218.46	35.90	1321.50	0.54	0.85
laboratory	Magallana gigas	7	0.060	88.0	40.2	25.6	0.910756	70.01700000	70.0280	113.43726910	1112.4395950	8.1	16	Extra Feeding								16000000	13.55	1.07	7.81	0.11	36.58	3.84	-3.77	584	28-32	2500	27	7.70	41.93	1080.29	1084.29	2371.12	86.94	2583.60	1.32	2.05
laboratory	Magallana gigas	7	0.051	72.5	46.6	23.9	0.855102	71.05400000	71.3280	109.74440950	1076.2250130	8.1	16	Extra Feeding								16000000	13.55	1.07	7.81	0.11	36.58	3.84	-3.77	584	28-32	2500	27	7.70	41.93	1080.29	1084.29	2371.12	86.94	2583.60	1.32	2.05
laboratory	Magallana gigas	7	0.033	64.6	36.8	21.5	0.763037	80.92700000	83.2190	82.58763896	809.9080696	8.1	16	Extra Feeding								16000000	13.55	1.07	7.81	0.11	36.58	3.84	-3.77	584	28-32	2500	27	7.70	41.93	1080.29	1084.29	2371.12	86.94	2583.60	1.32	2.05
laboratory	Magallana gigas	7	0.049	91.2	47.1	22.5	0.655278	73.74000000	80.9230	93.20857675	914.0638892	8.1	16	Extra Feeding								16000000	13.55	1.07	7.81	0.11	36.58	3.84	-3.77	584	28-32	2500	27	7.70	41.93	1080.29	1084.29	2371.12	86.94	2583.60	1.32	2.05
laboratory	Magallana gigas	8	0.058	85.6	38.7	27.5	0.908581	68.60800000	68.5590	118.24744100	1159.6112670	7.7	16	Extra Feeding								16000000	13.70	1.02	7.73	0.13	36.10	3.84	-5.97	821	33-36	1467	27	7.62	29.74	767.69	770.52	1395.04	42.22	1511.50	0.64	1.00
laboratory	Magallana gigas	8	0.049	67.0	62.3	25.2	0.692802	67.59800000	68.3110	120.44991410	1181.2101500	7.7	16	Extra Feeding								16000000	13.70	1.02	7.73	0.13	36.10	3.84	-5.97	821	33-36	1467	27	7.62	29.74	767.69	770.52	1395.04	42.22	1511.50	0.64	1.00
laboratory	Magallana gigas	8	0.060	90.9	48.7	21.5	0.789355	75.08000000	75.2590	98.43471251	965.3147734	7.7	16	Extra Feeding								16000000	13.70	1.02	7.73	0.13	36.10	3.84	-5.97	821	33-36	1467	27	7.62	29.74	767.69	770.52	1395.04	42.22	1511.50	0.64	1.00
laboratory	Magallana gigas	8	0.049	78.4	40.5	24.6	0.839678	78.14200000	78.1380	91.09282506	893.3154529	7.7	16	Extra Feeding								16000000	13.70	1.02	7.73	0.13	36.10	3.84	-5.97	821	33-36	1467	27	7.62	29.74	767.69	770.52	1395.04	42.22	1511.50	0.64	1.00
laboratory	Mytilus spp.	1	0.016	55.8	27.7	20.0	0.534290	70.84100000	66.3430	118.34534910	1160.5714180	8.1	14	control								8000000	11.45	0.86	7.91	0.08	28.42	4.07	-1.49	506	1-4	3096	27	7.79	48.08	1106.35	1110.55	2947.21	100.71	3179.80	1.56	2.49
laboratory	Mytilus spp.	1	0.014	53.0	27.1	18.5	0.512499	73.22500000	72.8290	104.29590510	1022.7934380	8.1	14	control								8000000	11.45	0.86	7.91	0.08	28.42	4.07	-1.49	506	1-4	3096	27	7.79	48.08	1106.35	1110.55	2947.21	100.71	3179.80	1.56	2.49
laboratory	Mytilus spp.	1	0.014	57.3	25.3	18.9	0.492539	68.89200000	68.1950	118.38853810	1160.9949570	8.1	14	control								8000000	11.45	0.86	7.91	0.08	28.42	4.07	-1.49	506	1-4	3096	27	7.79	48.08	1106.35	1110.55	2947.21	100.71	3179.80	1.56	2.49
laboratory	Mytilus spp.	1	0.017	57.8	26.7	19.7	0.564302	71.96090909	72.2745	106.94223280	1048.7450470	8.1	14	control								8000000	11.45	0.86	7.91	0.08	28.42	4.07	-1.49	506	1-4	3096	27	7.79	48.08	1106.35	1110.55	2947.21	100.71	3179.80	1.56	2.49
laboratory	Mytilus spp.	2	0.015	56.4	26.4	19.1	0.519610	70.88500000	71.7530	109.35447930	1072.4011040	7.7	14	control								8000000	11.70	0.84	7.71	0.07	32.78	1.42	-5.49	826	5-8	1214	27	7.60	28.00	666.03	668.55	1157.45	28.55	1239.80	0.44	0.69
laboratory	Mytilus spp.	2	0.015	55.8	27.4	19.8	0.506235	72.13700000	76.4280	100.88356310	989.3297941	7.7	14	control								8000000	11.70	0.84	7.71	0.07	32.78	1.42	-5.49	826	5-8	1214	27	7.60	28.00	666.03	668.55	1157.45	28.55	1239.80	0.44	0.69
laboratory	Mytilus spp.	2	0.016	57.2	25.1	18.4	0.572188	63.82600000	66.6236	130.79925210	1282.7024860	7.7	14	control								8000000	11.70	0.84	7.71	0.07	32.78	1.42	-5.49	826	5-8	1214	27	7.60	28.00	666.03	668.55	1157.45	28.55	1239.80	0.44	0.69
laboratory	Mytilus spp.	2	0.015	55.1	28.7	19.0	0.503521	68.58090909	69.1327	117.31248330	1150.4424640	7.7	14	control								8000000	11.70	0.84	7.71	0.07	32.78	1.42	-5.49	826	5-8	1214	27	7.60	28.00	666.03	668.55	1157.45	28.55	1239.80	0.44	0.69
laboratory	Mytilus spp.	3	0.014	54.1	25.5	18.3	0.524883	68.45785714	73.1750	111.03117930	1088.8439140	8.1	14	Extra Feeding								16000000	11.55	0.87	7.95	0.15	28.42	4.07	-1.76	661	9-12	3920	27	7.83	55.28	1276.18	1281.02	3724.63	140.09	4038.80	2.17	3.47
laboratory	Mytilus spp.	3	0.018	56.9	27.5	20.2	0.590215	75.16083333	80.9558	91.40952014	896.4211707	8.1	14	Extra Feeding								16000000	11.55	0.87	7.95	0.15	28.42	4.07	-1.76	661	9-12	3920	27	7.83	55.28	1276.18	1281.02	3724.63	140.09	4038.80	2.17	3.47
laboratory	Mytilus spp.	3	0.016	57.0	28.8	19.2	0.516277	72.74900000	81.3980	93.92695050	921.1087291	8.1	14	Extra Feeding								16000000	11.55	0.87	7.95	0.15	28.42	4.07	-1.76	661	9-12	3920	27	7.83	55.28	1276.18	1281.02	3724.63	140.09	4038.80	2.17	3.47
laboratory	Mytilus spp.	3	0.013	52.0	27.3	17.7	0.489169	73.00153846	77.3554	98.49367352	965.8929834	8.1	14	Extra Feeding								16000000	11.55	0.87	7.95	0.15	28.42	4.07	-1.76	661	9-12	3920	27	7.83	55.28	1276.18	1281.02	3724.63	140.09	4038.80	2.17	3.47
laboratory	Mytilus spp.	4	0.010	48.3	23.8	17.0	0.450649	75.17181818	80.2509	92.19895914	904.1629227	7.7	14	Extra Feeding								16000000	11.80	0.85	7.67	0.13	36.10	3.84	-6.56	951	13-16	1461	27	7.56	35.35	859.46	862.70	1391.27	34.38	1486.90	0.52	0.81
laboratory	Mytilus spp.	4	0.017	59.8	26.0	20.8	0.543542	76.60200000	77.1630	94.09829874	922.7890813	7.7	14	Extra Feeding								16000000	11.80	0.85	7.67	0.13	36.10	3.84	-6.56	951	13-16	1461	27	7.56	35.35	859.46	862.70	1391.27	34.38	1486.90	0.52	0.81
laboratory	Mytilus spp.	4	0.015	56.1	26.0	19.0	0.528591	48.65100000	48.4950	235.74486920	2311.8674220	7.7	14	Extra Feeding								16000000	11.80	0.85	7.67	0.13	36.10	3.84	-6.56	951	13-16	1461	27	7.56	35.35	859.46	862.70	1391.27	34.38	1486.90	0.52	0.81
laboratory	Mytilus spp.	4	0.012	51.2	25.9	16.9	0.482467	47.07400000	49.9720	236.44119750	2318.6960690	7.7	14	Extra Feeding								16000000	11.80	0.85	7.67	0.13	36.10	3.84	-6.56	951	13-16	1461	27	7.56	35.35	859.46	862.70	1391.27	34.38	1486.90	0.52	0.81
laboratory	Mytilus spp.	5	0.018	56.8	26.9	19.8	0.604005	82.47900000	93.6480	72.00938320	706.1708178	8.1	16	control								8000000	13.65	1.03	7.96	0.09	36.58	3.84	-1.57	521	17-20	3490	27	7.85	40.94	1058.06	1061.96	3278.56	170.50	3672.40	2.58	4.02
laboratory	Mytilus spp.	5	0.015	55.8	24.3	20.7	0.536111	72.40500000	74.1290	103.62732440	1016.2369010	8.1	16	control								8000000	13.65	1.03	7.96	0.09	36.58	3.84	-1.57	521	17-20	3490	27	7.85	40.94	1058.06	1061.96	3278.56	170.50	3672.40	2.58	4.02
laboratory	Mytilus spp.	5	0.015	54.5	27.3	19.1	0.525889	73.80727273	73.8809	101.99987730	1000.2770970	8.1	16	control								8000000	13.65	1.03	7.96	0.09	36.58	3.84	-1.57	521	17-20	3490	27	7.85	40.94	1058.06	1061.96	3278.56	170.50	3672.40	2.58	4.02
laboratory	Mytilus spp.	5	0.017	55.9	27.8	19.9	0.566289	74.17000000	74.4140	100.77389750	988.2543419	8.1	16	control								8000000	13.65	1.03	7.96	0.09	36.58	3.84	-1.57	521	17-20	3490	27	7.85	40.94	1058.06	1061.96	3278.56	170.50	3672.40	2.58	4.02
laboratory	Mytilus spp.	6	0.015	52.5	26.4	19.5	0.554195	72.12250000	73.7067	104.62928190	1026.0627470	7.7	16	control								8000000	13.60	1.03	7.72	0.93	36.10	3.84	-2.67	800	21-24	1281	27	7.61	26.65	685.69	688.22	1218.46	35.90	1321.50	0.54	0.85
laboratory	Mytilus spp.	6	0.014	54.2	26.8	19.8	0.493507	69.17500000	76.9580	104.47876830	1024.5867130	7.7	16	control								8000000	13.60	1.03	7.72	0.93	36.10	3.84	-2.67	800	21-24	1281	27	7.61	26.65	685.69	688.22	1218.46	35.90	1321.50	0.54	0.85
laboratory	Mytilus spp.	6	0.016	57.4	27.4	18.1	0.540387	71.63181818	78.3818	99.06261252	971.4723691	7.7	16	control								8000000	13.60	1.03	7.72	0.93	36.10	3.84	-2.67	800	21-24	1281	27	7.61	26.65	685.69	688.22	1218.46	35.90	1321.50	0.54	0.85
laboratory	Mytilus spp.	6	0.017	56.5	27.3	19.9	0.566997	68.68545455	72.8282	111.19023080	1090.4036770	7.7	16	control								8000000	13.60	1.03	7.72	0.93	36.10	3.84	-2.67	800	21-24	1281	27	7.61	26.65	685.69	688.22	1218.46	35.90	1321.50	0.54	0.85
laboratory	Mytilus spp.	7	0.020	61.0	28.2	21.3	0.590625	69.47800000	71.1680	112.48611430	1103.1119530	8.1	16	Extra Feeding								16000000	13.55	1.07	7.81	0.11	36.58	3.84	-3.77	584	28-32	2500	27	7.70	41.93	1080.29	1084.29	2371.12	86.94	2583.60	1.32	2.05
laboratory	Mytilus spp.	7	0.013	53.5	23.9	17.4	0.523265	64.57818182	81.7755	105.32269490	1032.8628060	8.1	16	Extra Feeding								16000000	13.55	1.07	7.81	0.11	36.58	3.84	-3.77	584	28-32	2500	27	7.70	41.93	1080.29	1084.29	2371.12	86.94	2583.60	1.32	2.05
laboratory	Mytilus spp.	7	0.018	59.4	26.0	20.9	0.578304	71.16600000	71.3740	109.50107730	1073.8387400	8.1	16	Extra Feeding								16000000	13.55	1.07	7.81	0.11	36.58	3.84	-3.77	584	28-32	2500	27	7.70	41.93	1080.29	1084.29	2371.12	86.94	2583.60	1.32	2.05
laboratory	Mytilus spp.	7	0.018	58.5	27.5	19.2	0.584039	66.38200000	68.8360	121.72086380	1193.6739090	8.1	16	Extra Feeding								16000000	13.55	1.07	7.81	0.11	36.58	3.84	-3.77	584	28-32	2500	27	7.70	41.93	1080.29	1084.29	2371.12	86.94	2583.60	1.32	2.05
laboratory	Mytilus spp.	8	0.013	51.9	24.8	19.2	0.508428	74.79181818	74.8827	99.31055259	973.9038306	7.7	16	Extra Feeding								16000000	13.70	1.02	7.73	0.13	36.10	3.84	-5.97	821	33-36	1467	27	7.62	29.74	767.69	770.52	1395.04	42.22	1511.50	0.64	1.00
laboratory	Mytilus spp.	8	0.014	53.5	25.9	18.3	0.525316	73.78700000	76.1050	99.04622801	971.3116919	7.7	16	Extra Feeding								16000000	13.70	1.02	7.73	0.13	36.10	3.84	-5.97	821	33-36	1467	27	7.62	29.74	767.69	770.52	1395.04	42.22	1511.50	0.64	1.00
laboratory	Mytilus spp.	8	0.015	56.2	26.0	18.4	0.533453	69.79500000	73.5780	108.30754060	1062.1341430	7.7	16	Extra Feeding								16000000	13.70	1.02	7.73	0.13	36.10	3.84	-5.97	821	33-36	1467	27	7.62	29.74	767.69	770.52	1395.04	42.22	1511.50	0.64	1.00
laboratory	Mytilus spp.	8	0.018	60.8	29.1	20.0	0.533764	71.35000000	75.2164	103.63929820	1016.3543240	7.7	16	Extra Feeding								16000000	13.70	1.02	7.73	0.13	36.10	3.84	-5.97	821	33-36	1467	27	7.62	29.74	767.69	770.52	1395.04	42.22	1511.50	0.64	1.00
laboratory	Magallana gigas	1										8.1	14	control	1	-21.07	7.98					8000000	11.45	0.86	7.91	0.08	28.42	4.07	-1.49	506	1-4	3096	27	7.79	48.08	1106.35	1110.55	2947.21	100.71	3179.80	1.56	2.49
laboratory	Magallana gigas	1										8.1	14	control	1	-21.57	8.61					8000000	11.45	0.86	7.91	0.08	28.42	4.07	-1.49	506	1-4	3096	27	7.79	48.08	1106.35	1110.55	2947.21	100.71	3179.80	1.56	2.49
laboratory	Magallana gigas	1										8.1	14	control	2	-21.37	8.02					8000000	11.45	0.86	7.91	0.08	28.42	4.07	-1.49	506	1-4	3096	27	7.79	48.08	1106.35	1110.55	2947.21	100.71	3179.80	1.56	2.49
laboratory	Magallana gigas	1										8.1	14	control	2	-21.39	8.05					8000000	11.45	0.86	7.91	0.08	28.42	4.07	-1.49	506	1-4	3096	27	7.79	48.08	1106.35	1110.55	2947.21	100.71	3179.80	1.56	2.49
laboratory	Magallana gigas	2										7.7	14	control	3	-21.81	7.60					8000000	11.70	0.84	7.71	0.07	32.78	1.42	-5.49	826	5-8	1214	27	7.60	28.00	666.03	668.55	1157.45	28.55	1239.80	0.44	0.69
laboratory	Magallana gigas	2										7.7	14	control	3	-21.02	7.74					8000000	11.70	0.84	7.71	0.07	32.78	1.42	-5.49	826	5-8	1214	27	7.60	28.00	666.03	668.55	1157.45	28.55	1239.80	0.44	0.69
laboratory	Magallana gigas	2										7.7	14	control	4	-21.88	7.67					8000000	11.70	0.84	7.71	0.07	32.78	1.42	-5.49	826	5-8	1214	27	7.60	28.00	666.03	668.55	1157.45	28.55	1239.80	0.44	0.69
laboratory	Magallana gigas	2										7.7	14	control	4	-20.69	8.29					8000000	11.70	0.84	7.71	0.07	32.78	1.42	-5.49	826	5-8	1214	27	7.60	28.00	666.03	668.55	1157.45	28.55	1239.80	0.44	0.69
laboratory	Magallana gigas	3										8.1	14	Extra Feeding	5	-21.19	8.39					16000000	11.55	0.87	7.95	0.15	28.42	4.07	-1.76	661	9-12	3920	27	7.83	55.28	1276.18	1281.02	3724.63	140.09	4038.80	2.17	3.47
laboratory	Magallana gigas	3										8.1	14	Extra Feeding	5	-22.23	7.07					16000000	11.55	0.87	7.95	0.15	28.42	4.07	-1.76	661	9-12	3920	27	7.83	55.28	1276.18	1281.02	3724.63	140.09	4038.80	2.17	3.47
laboratory	Magallana gigas	3										8.1	14	Extra Feeding	6	-21.03	8.44					16000000	11.55	0.87	7.95	0.15	28.42	4.07	-1.76	661	9-12	3920	27	7.83	55.28	1276.18	1281.02	3724.63	140.09	4038.80	2.17	3.47
laboratory	Magallana gigas	3										8.1	14	Extra Feeding	6	-20.66	7.89					16000000	11.55	0.87	7.95	0.15	28.42	4.07	-1.76	661	9-12	3920	27	7.83	55.28	1276.18	1281.02	3724.63	140.09	4038.80	2.17	3.47
laboratory	Magallana gigas	4										7.7	14	Extra Feeding	7	-21.41	7.52					16000000	11.80	0.85	7.67	0.13	36.10	3.84	-6.56	951	13-16	1461	27	7.56	35.35	859.46	862.70	1391.27	34.38	1486.90	0.52	0.81
laboratory	Magallana gigas	4										7.7	14	Extra Feeding	7	-21.85	7.05					16000000	11.80	0.85	7.67	0.13	36.10	3.84	-6.56	951	13-16	1461	27	7.56	35.35	859.46	862.70	1391.27	34.38	1486.90	0.52	0.81
laboratory	Magallana gigas	4										7.7	14	Extra Feeding	8	-20.76	7.70					16000000	11.80	0.85	7.67	0.13	36.10	3.84	-6.56	951	13-16	1461	27	7.56	35.35	859.46	862.70	1391.27	34.38	1486.90	0.52	0.81
laboratory	Magallana gigas	4										7.7	14	Extra Feeding	8	-21.23	7.93					16000000	11.80	0.85	7.67	0.13	36.10	3.84	-6.56	951	13-16	1461	27	7.56	35.35	859.46	862.70	1391.27	34.38	1486.90	0.52	0.81
laboratory	Magallana gigas	5										8.1	16	control	9	-21.53	8.30					8000000	13.65	1.03	7.96	0.09	36.58	3.84	-1.57	521	17-20	3490	27	7.85	40.94	1058.06	1061.96	3278.56	170.50	3672.40	2.58	4.02
laboratory	Magallana gigas	5										8.1	16	control	9	-20.80	7.85					8000000	13.65	1.03	7.96	0.09	36.58	3.84	-1.57	521	17-20	3490	27	7.85	40.94	1058.06	1061.96	3278.56	170.50	3672.40	2.58	4.02
laboratory	Magallana gigas	5										8.1	16	control	10	-21.05	7.45					8000000	13.65	1.03	7.96	0.09	36.58	3.84	-1.57	521	17-20	3490	27	7.85	40.94	1058.06	1061.96	3278.56	170.50	3672.40	2.58	4.02
laboratory	Magallana gigas	5										8.1	16	control	10	-20.66	8.08					8000000	13.65	1.03	7.96	0.09	36.58	3.84	-1.57	521	17-20	3490	27	7.85	40.94	1058.06	1061.96	3278.56	170.50	3672.40	2.58	4.02
laboratory	Magallana gigas	6										7.7	16	control	11	-21.68	8.16					8000000	13.60	1.03	7.72	0.93	36.10	3.84	-2.67	800	21-24	1281	27	7.61	26.65	685.69	688.22	1218.46	35.90	1321.50	0.54	0.85
laboratory	Magallana gigas	6										7.7	16	control	11	-21.01	7.97					8000000	13.60	1.03	7.72	0.93	36.10	3.84	-2.67	800	21-24	1281	27	7.61	26.65	685.69	688.22	1218.46	35.90	1321.50	0.54	0.85
laboratory	Magallana gigas	6										7.7	16	control	12	-21.78	7.50					8000000	13.60	1.03	7.72	0.93	36.10	3.84	-2.67	800	21-24	1281	27	7.61	26.65	685.69	688.22	1218.46	35.90	1321.50	0.54	0.85
laboratory	Magallana gigas	6										7.7	16	control	12	-20.86	7.64					8000000	13.60	1.03	7.72	0.93	36.10	3.84	-2.67	800	21-24	1281	27	7.61	26.65	685.69	688.22	1218.46	35.90	1321.50	0.54	0.85
laboratory	Magallana gigas	7										8.1	16	Extra Feeding	13	-20.46	7.94					16000000	13.55	1.07	7.81	0.11	36.58	3.84	-3.77	584	28-32	2500	27	7.70	41.93	1080.29	1084.29	2371.12	86.94	2583.60	1.32	2.05
laboratory	Magallana gigas	7										8.1	16	Extra Feeding	13	-21.67	7.93					16000000	13.55	1.07	7.81	0.11	36.58	3.84	-3.77	584	28-32	2500	27	7.70	41.93	1080.29	1084.29	2371.12	86.94	2583.60	1.32	2.05
laboratory	Magallana gigas	7										8.1	16	Extra Feeding	14	-21.39	7.60					16000000	13.55	1.07	7.81	0.11	36.58	3.84	-3.77	584	28-32	2500	27	7.70	41.93	1080.29	1084.29	2371.12	86.94	2583.60	1.32	2.05
laboratory	Magallana gigas	7										8.1	16	Extra Feeding	14	-20.59	7.94					16000000	13.55	1.07	7.81	0.11	36.58	3.84	-3.77	584	28-32	2500	27	7.70	41.93	1080.29	1084.29	2371.12	86.94	2583.60	1.32	2.05
laboratory	Magallana gigas	8										7.7	16	Extra Feeding	15	-21.46	7.55					16000000	13.70	1.02	7.73	0.13	36.10	3.84	-5.97	821	33-36	1467	27	7.62	29.74	767.69	770.52	1395.04	42.22	1511.50	0.64	1.00
laboratory	Magallana gigas	8										7.7	16	Extra Feeding	15	-21.47	7.74					16000000	13.70	1.02	7.73	0.13	36.10	3.84	-5.97	821	33-36	1467	27	7.62	29.74	767.69	770.52	1395.04	42.22	1511.50	0.64	1.00
laboratory	Magallana gigas	8										7.7	16	Extra Feeding	16	-20.75	7.97					16000000	13.70	1.02	7.73	0.13	36.10	3.84	-5.97	821	33-36	1467	27	7.62	29.74	767.69	770.52	1395.04	42.22	1511.50	0.64	1.00
laboratory	Magallana gigas	8										7.7	16	Extra Feeding	16	-20.81	7.54					16000000	13.70	1.02	7.73	0.13	36.10	3.84	-5.97	821	33-36	1467	27	7.62	29.74	767.69	770.52	1395.04	42.22	1511.50	0.64	1.00
laboratory	Mytilus spp.	1										8.1	14	control	1	-19.63	7.08					8000000	11.45	0.86	7.91	0.08	28.42	4.07	-1.49	506	1-4	3096	27	7.79	48.08	1106.35	1110.55	2947.21	100.71	3179.80	1.56	2.49
laboratory	Mytilus spp.	1										8.1	14	control	1	-20.30	6.98					8000000	11.45	0.86	7.91	0.08	28.42	4.07	-1.49	506	1-4	3096	27	7.79	48.08	1106.35	1110.55	2947.21	100.71	3179.80	1.56	2.49
laboratory	Mytilus spp.	1										8.1	14	control	2	-20.09	6.84					8000000	11.45	0.86	7.91	0.08	28.42	4.07	-1.49	506	1-4	3096	27	7.79	48.08	1106.35	1110.55	2947.21	100.71	3179.80	1.56	2.49
laboratory	Mytilus spp.	1										8.1	14	control	2	-20.39	7.32					8000000	11.45	0.86	7.91	0.08	28.42	4.07	-1.49	506	1-4	3096	27	7.79	48.08	1106.35	1110.55	2947.21	100.71	3179.80	1.56	2.49
laboratory	Mytilus spp.	2										7.7	14	control	3	-20.22	7.49					8000000	11.70	0.84	7.71	0.07	32.78	1.42	-5.49	826	5-8	1214	27	7.60	28.00	666.03	668.55	1157.45	28.55	1239.80	0.44	0.69
laboratory	Mytilus spp.	2										7.7	14	control	3	-20.24	6.96					8000000	11.70	0.84	7.71	0.07	32.78	1.42	-5.49	826	5-8	1214	27	7.60	28.00	666.03	668.55	1157.45	28.55	1239.80	0.44	0.69
laboratory	Mytilus spp.	2										7.7	14	control	4	-19.44	6.93					8000000	11.70	0.84	7.71	0.07	32.78	1.42	-5.49	826	5-8	1214	27	7.60	28.00	666.03	668.55	1157.45	28.55	1239.80	0.44	0.69
laboratory	Mytilus spp.	2										7.7	14	control	4	-18.89	7.53					8000000	11.70	0.84	7.71	0.07	32.78	1.42	-5.49	826	5-8	1214	27	7.60	28.00	666.03	668.55	1157.45	28.55	1239.80	0.44	0.69
laboratory	Mytilus spp.	3										8.1	14	Extra Feeding	5	-20.27	7.93					16000000	11.55	0.87	7.95	0.15	28.42	4.07	-1.76	661	9-12	3920	27	7.83	55.28	1276.18	1281.02	3724.63	140.09	4038.80	2.17	3.47
laboratory	Mytilus spp.	3										8.1	14	Extra Feeding	5	-20.50	6.05					16000000	11.55	0.87	7.95	0.15	28.42	4.07	-1.76	661	9-12	3920	27	7.83	55.28	1276.18	1281.02	3724.63	140.09	4038.80	2.17	3.47
laboratory	Mytilus spp.	3										8.1	14	Extra Feeding	6	-19.51	7.75					16000000	11.55	0.87	7.95	0.15	28.42	4.07	-1.76	661	9-12	3920	27	7.83	55.28	1276.18	1281.02	3724.63	140.09	4038.80	2.17	3.47
laboratory	Mytilus spp.	3										8.1	14	Extra Feeding	6	-20.27	7.37					16000000	11.55	0.87	7.95	0.15	28.42	4.07	-1.76	661	9-12	3920	27	7.83	55.28	1276.18	1281.02	3724.63	140.09	4038.80	2.17	3.47
laboratory	Mytilus spp.	4										7.7	14	Extra Feeding	7	-20.35	6.61					16000000	11.80	0.85	7.67	0.13	36.10	3.84	-6.56	951	13-16	1461	27	7.56	35.35	859.46	862.70	1391.27	34.38	1486.90	0.52	0.81
laboratory	Mytilus spp.	4										7.7	14	Extra Feeding	7	-19.78	7.78					16000000	11.80	0.85	7.67	0.13	36.10	3.84	-6.56	951	13-16	1461	27	7.56	35.35	859.46	862.70	1391.27	34.38	1486.90	0.52	0.81
laboratory	Mytilus spp.	4										7.7	14	Extra Feeding	8	-20.85	4.47					16000000	11.80	0.85	7.67	0.13	36.10	3.84	-6.56	951	13-16	1461	27	7.56	35.35	859.46	862.70	1391.27	34.38	1486.90	0.52	0.81
laboratory	Mytilus spp.	4										7.7	14	Extra Feeding	8	-20.33	7.30					16000000	11.80	0.85	7.67	0.13	36.10	3.84	-6.56	951	13-16	1461	27	7.56	35.35	859.46	862.70	1391.27	34.38	1486.90	0.52	0.81
laboratory	Mytilus spp.	5										8.1	16	control	9	-19.60	7.18					8000000	13.65	1.03	7.96	0.09	36.58	3.84	-1.57	521	17-20	3490	27	7.85	40.94	1058.06	1061.96	3278.56	170.50	3672.40	2.58	4.02
laboratory	Mytilus spp.	5										8.1	16	control	9	-19.41	7.75					8000000	13.65	1.03	7.96	0.09	36.58	3.84	-1.57	521	17-20	3490	27	7.85	40.94	1058.06	1061.96	3278.56	170.50	3672.40	2.58	4.02
laboratory	Mytilus spp.	5										8.1	16	control	10	-19.72	8.01					8000000	13.65	1.03	7.96	0.09	36.58	3.84	-1.57	521	17-20	3490	27	7.85	40.94	1058.06	1061.96	3278.56	170.50	3672.40	2.58	4.02
laboratory	Mytilus spp.	5										8.1	16	control	10	-19.53	8.37					8000000	13.65	1.03	7.96	0.09	36.58	3.84	-1.57	521	17-20	3490	27	7.85	40.94	1058.06	1061.96	3278.56	170.50	3672.40	2.58	4.02
laboratory	Mytilus spp.	6										7.7	16	control	11	-20.10	7.60					8000000	13.60	1.03	7.72	0.93	36.10	3.84	-2.67	800	21-24	1281	27	7.61	26.65	685.69	688.22	1218.46	35.90	1321.50	0.54	0.85
laboratory	Mytilus spp.	6										7.7	16	control	11	-20.22	6.39					8000000	13.60	1.03	7.72	0.93	36.10	3.84	-2.67	800	21-24	1281	27	7.61	26.65	685.69	688.22	1218.46	35.90	1321.50	0.54	0.85
laboratory	Mytilus spp.	6										7.7	16	control	12	-19.60	4.83					8000000	13.60	1.03	7.72	0.93	36.10	3.84	-2.67	800	21-24	1281	27	7.61	26.65	685.69	688.22	1218.46	35.90	1321.50	0.54	0.85
laboratory	Mytilus spp.	6										7.7	16	control	12	-20.51	8.00					8000000	13.60	1.03	7.72	0.93	36.10	3.84	-2.67	800	21-24	1281	27	7.61	26.65	685.69	688.22	1218.46	35.90	1321.50	0.54	0.85
laboratory	Mytilus spp.	7										8.1	16	Extra Feeding	13	-19.98	7.49					16000000	13.55	1.07	7.81	0.11	36.58	3.84	-3.77	584	28-32	2500	27	7.70	41.93	1080.29	1084.29	2371.12	86.94	2583.60	1.32	2.05
laboratory	Mytilus spp.	7										8.1	16	Extra Feeding	13	-19.88	6.80					16000000	13.55	1.07	7.81	0.11	36.58	3.84	-3.77	584	28-32	2500	27	7.70	41.93	1080.29	1084.29	2371.12	86.94	2583.60	1.32	2.05
laboratory	Mytilus spp.	7										8.1	16	Extra Feeding	14	-20.47	8.21					16000000	13.55	1.07	7.81	0.11	36.58	3.84	-3.77	584	28-32	2500	27	7.70	41.93	1080.29	1084.29	2371.12	86.94	2583.60	1.32	2.05
laboratory	Mytilus spp.	7										8.1	16	Extra Feeding	14	-19.59	7.43					16000000	13.55	1.07	7.81	0.11	36.58	3.84	-3.77	584	28-32	2500	27	7.70	41.93	1080.29	1084.29	2371.12	86.94	2583.60	1.32	2.05
laboratory	Mytilus spp.	8										7.7	16	Extra Feeding	15	-18.96	7.21					16000000	13.70	1.02	7.73	0.13	36.10	3.84	-5.97	821	33-36	1467	27	7.62	29.74	767.69	770.52	1395.04	42.22	1511.50	0.64	1.00
laboratory	Mytilus spp.	8										7.7	16	Extra Feeding	15	-19.56	7.85					16000000	13.70	1.02	7.73	0.13	36.10	3.84	-5.97	821	33-36	1467	27	7.62	29.74	767.69	770.52	1395.04	42.22	1511.50	0.64	1.00
laboratory	Mytilus spp.	8										7.7	16	Extra Feeding	16	-19.45	7.46					16000000	13.70	1.02	7.73	0.13	36.10	3.84	-5.97	821	33-36	1467	27	7.62	29.74	767.69	770.52	1395.04	42.22	1511.50	0.64	1.00
laboratory	Mytilus spp.	8										7.7	16	Extra Feeding	16	-19.96	7.47					16000000	13.70	1.02	7.73	0.13	36.10	3.84	-5.97	821	33-36	1467	27	7.62	29.74	767.69	770.52	1395.04	42.22	1511.50	0.64	1.00
laboratory	Magallana gigas	1										8.1	14	control	1			-1.38	1.09			8000000	11.45	0.86	7.91	0.08	28.42	4.07	-1.49	506	1-4	3096	27	7.79	48.08	1106.35	1110.55	2947.21	100.71	3179.80	1.56	2.49
laboratory	Magallana gigas	1										8.1	14	control	1			-0.84	0.42			8000000	11.45	0.86	7.91	0.08	28.42	4.07	-1.49	506	1-4	3096	27	7.79	48.08	1106.35	1110.55	2947.21	100.71	3179.80	1.56	2.49
laboratory	Magallana gigas	1										8.1	14	control	2			-0.96	0.82			8000000	11.45	0.86	7.91	0.08	28.42	4.07	-1.49	506	1-4	3096	27	7.79	48.08	1106.35	1110.55	2947.21	100.71	3179.80	1.56	2.49
laboratory	Magallana gigas	1										8.1	14	control	2			-1.72	0.14			8000000	11.45	0.86	7.91	0.08	28.42	4.07	-1.49	506	1-4	3096	27	7.79	48.08	1106.35	1110.55	2947.21	100.71	3179.80	1.56	2.49
laboratory	Magallana gigas	2										7.7	14	control	3				0.32			8000000	11.70	0.84	7.71	0.07	32.78	1.42	-5.49	826	5-8	1214	27	7.60	28.00	666.03	668.55	1157.45	28.55	1239.80	0.44	0.69
laboratory	Magallana gigas	2										7.7	14	control	3			-0.72	0.15			8000000	11.70	0.84	7.71	0.07	32.78	1.42	-5.49	826	5-8	1214	27	7.60	28.00	666.03	668.55	1157.45	28.55	1239.80	0.44	0.69
laboratory	Magallana gigas	2										7.7	14	control	4			-0.55	-0.01			8000000	11.70	0.84	7.71	0.07	32.78	1.42	-5.49	826	5-8	1214	27	7.60	28.00	666.03	668.55	1157.45	28.55	1239.80	0.44	0.69
laboratory	Magallana gigas	2										7.7	14	control	4			-0.37	0.84			8000000	11.70	0.84	7.71	0.07	32.78	1.42	-5.49	826	5-8	1214	27	7.60	28.00	666.03	668.55	1157.45	28.55	1239.80	0.44	0.69
laboratory	Magallana gigas	3										8.1	14	Extra Feeding	5			-1.01	0.63			16000000	11.55	0.87	7.95	0.15	28.42	4.07	-1.76	661	9-12	3920	27	7.83	55.28	1276.18	1281.02	3724.63	140.09	4038.80	2.17	3.47
laboratory	Magallana gigas	3										8.1	14	Extra Feeding	5			-1.56	0.51			16000000	11.55	0.87	7.95	0.15	28.42	4.07	-1.76	661	9-12	3920	27	7.83	55.28	1276.18	1281.02	3724.63	140.09	4038.80	2.17	3.47
laboratory	Magallana gigas	3										8.1	14	Extra Feeding	6			-1.08	0.53			16000000	11.55	0.87	7.95	0.15	28.42	4.07	-1.76	661	9-12	3920	27	7.83	55.28	1276.18	1281.02	3724.63	140.09	4038.80	2.17	3.47
laboratory	Magallana gigas	3										8.1	14	Extra Feeding	6							16000000	11.55	0.87	7.95	0.15	28.42	4.07	-1.76	661	9-12	3920	27	7.83	55.28	1276.18	1281.02	3724.63	140.09	4038.80	2.17	3.47
laboratory	Magallana gigas	4										7.7	14	Extra Feeding	7			-0.55	0.66			16000000	11.80	0.85	7.67	0.13	36.10	3.84	-6.56	951	13-16	1461	27	7.56	35.35	859.46	862.70	1391.27	34.38	1486.90	0.52	0.81
laboratory	Magallana gigas	4										7.7	14	Extra Feeding	7							16000000	11.80	0.85	7.67	0.13	36.10	3.84	-6.56	951	13-16	1461	27	7.56	35.35	859.46	862.70	1391.27	34.38	1486.90	0.52	0.81
laboratory	Magallana gigas	4										7.7	14	Extra Feeding	8			-18.87	0.34			16000000	11.80	0.85	7.67	0.13	36.10	3.84	-6.56	951	13-16	1461	27	7.56	35.35	859.46	862.70	1391.27	34.38	1486.90	0.52	0.81
laboratory	Magallana gigas	4										7.7	14	Extra Feeding	8			-0.66	-1.72			16000000	11.80	0.85	7.67	0.13	36.10	3.84	-6.56	951	13-16	1461	27	7.56	35.35	859.46	862.70	1391.27	34.38	1486.90	0.52	0.81
laboratory	Magallana gigas	5										8.1	16	control	9			-0.82	0.14			8000000	13.65	1.03	7.96	0.09	36.58	3.84	-1.57	521	17-20	3490	27	7.85	40.94	1058.06	1061.96	3278.56	170.50	3672.40	2.58	4.02
laboratory	Magallana gigas	5										8.1	16	control	9			-0.84	0.02			8000000	13.65	1.03	7.96	0.09	36.58	3.84	-1.57	521	17-20	3490	27	7.85	40.94	1058.06	1061.96	3278.56	170.50	3672.40	2.58	4.02
laboratory	Magallana gigas	5										8.1	16	control	10			-1.23	1.05			8000000	13.65	1.03	7.96	0.09	36.58	3.84	-1.57	521	17-20	3490	27	7.85	40.94	1058.06	1061.96	3278.56	170.50	3672.40	2.58	4.02
laboratory	Magallana gigas	5										8.1	16	control	10			-4.88	1.29			8000000	13.65	1.03	7.96	0.09	36.58	3.84	-1.57	521	17-20	3490	27	7.85	40.94	1058.06	1061.96	3278.56	170.50	3672.40	2.58	4.02
laboratory	Magallana gigas	6										7.7	16	control	11			-2.40	0.13			8000000	13.60	1.03	7.72	0.93	36.10	3.84	-2.67	800	21-24	1281	27	7.61	26.65	685.69	688.22	1218.46	35.90	1321.50	0.54	0.85
laboratory	Magallana gigas	6										7.7	16	control	11			-1.34	0.16			8000000	13.60	1.03	7.72	0.93	36.10	3.84	-2.67	800	21-24	1281	27	7.61	26.65	685.69	688.22	1218.46	35.90	1321.50	0.54	0.85
laboratory	Magallana gigas	6										7.7	16	control	12			-0.79	0.37			8000000	13.60	1.03	7.72	0.93	36.10	3.84	-2.67	800	21-24	1281	27	7.61	26.65	685.69	688.22	1218.46	35.90	1321.50	0.54	0.85
laboratory	Magallana gigas	6										7.7	16	control	12			-0.05	6.35			8000000	13.60	1.03	7.72	0.93	36.10	3.84	-2.67	800	21-24	1281	27	7.61	26.65	685.69	688.22	1218.46	35.90	1321.50	0.54	0.85
laboratory	Magallana gigas	7										8.1	16	Extra Feeding	13			-0.93	0.94			16000000	13.55	1.07	7.81	0.11	36.58	3.84	-3.77	584	28-32	2500	27	7.70	41.93	1080.29	1084.29	2371.12	86.94	2583.60	1.32	2.05
laboratory	Magallana gigas	7										8.1	16	Extra Feeding	13			1.89	0.51			16000000	13.55	1.07	7.81	0.11	36.58	3.84	-3.77	584	28-32	2500	27	7.70	41.93	1080.20	1084.19	2371.12	86.95	2583.61	1.32	2.05
laboratory	Magallana gigas	7										8.1	16	Extra Feeding	14			-0.94	0.56			16000000	13.55	1.07	7.81	0.11	36.58	3.84	-3.77	584	28-32	2500	27	7.70	41.93	1080.20	1084.19	2371.12	86.95	2583.61	1.32	2.05
laboratory	Magallana gigas	7										8.1	16	Extra Feeding	14			-1.12	0.59			16000000	13.55	1.07	7.81	0.11	36.58	3.84	-3.77	584	28-32	2500	27	7.70	41.93	1080.20	1084.19	2371.12	86.95	2583.61	1.32	2.05
laboratory	Magallana gigas	8										7.7	16	Extra Feeding	15			-1.20	0.30			16000000	13.70	1.02	7.73	0.13	36.10	3.84	-5.97	821	33-36	1467	27	7.62	29.75	767.95	770.78	1395.04	42.21	1511.47	0.64	1.00
laboratory	Magallana gigas	8										7.7	16	Extra Feeding	15			-0.72	0.41			16000000	13.70	1.02	7.73	0.13	36.10	3.84	-5.97	821	33-36	1467	27	7.62	29.75	767.95	770.78	1395.04	42.21	1511.47	0.64	1.00
laboratory	Magallana gigas	8										7.7	16	Extra Feeding	16				0.06			16000000	13.70	1.02	7.73	0.13	36.10	3.84	-5.97	821	33-36	1467	27	7.62	29.75	767.95	770.78	1395.04	42.21	1511.47	0.64	1.00
laboratory	Magallana gigas	8										7.7	16	Extra Feeding	16			-0.38	0.81			16000000	13.70	1.02	7.73	0.13	36.10	3.84	-5.97	821	33-36	1467	27	7.62	29.75	767.95	770.78	1395.04	42.21	1511.47	0.64	1.00
laboratory	Mytilus spp.	1										8.1	14	control	1			-1.42	0.85	-2.13	0.06	8000000	11.45	0.86	7.91	0.08	28.42	4.07	-1.49	506	1-4	3096	27	7.79	48.07	1106.23	1110.43	2947.21	100.72	3179.82	1.56	2.49
laboratory	Mytilus spp.	1										8.1	14	control	1			-1.45	0.87			8000000	11.45	0.86	7.91	0.08	28.42	4.07	-1.49	506	1-4	3096	27	7.79	48.07	1106.23	1110.43	2947.21	100.72	3179.82	1.56	2.49
laboratory	Mytilus spp.	1										8.1	14	control	2			-2.09	0.95	-2.45	0.43	8000000	11.45	0.86	7.91	0.08	28.42	4.07	-1.49	506	1-4	3096	27	7.79	48.07	1106.23	1110.43	2947.21	100.72	3179.82	1.56	2.49
laboratory	Mytilus spp.	1										8.1	14	control	2			-1.39	1.00	-1.96	0.20	8000000	11.45	0.86	7.91	0.08	28.42	4.07	-1.49	506	1-4	3096	27	7.79	48.07	1106.23	1110.43	2947.21	100.72	3179.82	1.56	2.49
laboratory	Mytilus spp.	2										7.7	14	control	3			-0.76		-2.48	0.38	8000000	11.70	0.84	7.71	0.07	32.78	1.42	-5.49	826	5-8	1214	27	7.60	28.01	666.11	668.63	1157.45	28.54	1239.79	0.44	0.69
laboratory	Mytilus spp.	2										7.7	14	control	3			-2.29	0.19	-4.08	0.44	8000000	11.70	0.84	7.71	0.07	32.78	1.42	-5.49	826	5-8	1214	27	7.60	28.01	666.11	668.63	1157.45	28.54	1239.79	0.44	0.69
laboratory	Mytilus spp.	2										7.7	14	control	4			-0.76	-0.35	-3.14	0.30	8000000	11.70	0.84	7.71	0.07	32.78	1.42	-5.49	826	5-8	1214	27	7.60	28.01	666.11	668.63	1157.45	28.54	1239.79	0.44	0.69
laboratory	Mytilus spp.	2										7.7	14	control	4			-0.22	-0.82	-2.54	0.17	8000000	11.70	0.84	7.71	0.07	32.78	1.42	-5.49	826	5-8	1214	27	7.60	28.01	666.11	668.63	1157.45	28.54	1239.79	0.44	0.69
laboratory	Mytilus spp.	3										8.1	14	Extra Feeding	5			-1.45	1.42	-2.32	0.59	16000000	11.55	0.87	7.95	0.15	28.42	4.07	-1.76	661	9-12	3920	27	7.83	55.28	1276.29	1281.14	3724.64	140.08	4038.78	2.17	3.47
laboratory	Mytilus spp.	3										8.1	14	Extra Feeding	5					-1.94	0.08	16000000	11.55	0.87	7.95	0.15	28.42	4.07	-1.76	661	9-12	3920	27	7.83	55.28	1276.29	1281.14	3724.64	140.08	4038.78	2.17	3.47
laboratory	Mytilus spp.	3										8.1	14	Extra Feeding	6			-1.92	-0.06	-2.59	0.06	16000000	11.55	0.87	7.95	0.15	28.42	4.07	-1.76	661	9-12	3920	27	7.83	55.28	1276.29	1281.14	3724.64	140.08	4038.78	2.17	3.47
laboratory	Mytilus spp.	3										8.1	14	Extra Feeding	6			-2.40	0.18	-2.51	-0.15	16000000	11.55	0.87	7.95	0.15	28.42	4.07	-1.76	661	9-12	3920	27	7.83	55.28	1276.29	1281.14	3724.64	140.08	4038.78	2.17	3.47
laboratory	Mytilus spp.	4										7.7	14	Extra Feeding	7			-0.70	0.23	-2.72	0.10	16000000	11.80	0.85	7.67	0.13	36.10	3.84	-6.56	951	13-16	1461	27	7.56	35.36	859.69	862.94	1391.27	34.37	1486.87	0.52	0.81
laboratory	Mytilus spp.	4										7.7	14	Extra Feeding	7			-1.09	1.23	-3.19	-0.77	16000000	11.80	0.85	7.67	0.13	36.10	3.84	-6.56	951	13-16	1461	27	7.56	35.36	859.69	862.94	1391.27	34.37	1486.87	0.52	0.81
laboratory	Mytilus spp.	4										7.7	14	Extra Feeding	8			-13.76	-100.96	-3.08	0.15	16000000	11.80	0.85	7.67	0.13	36.10	3.84	-6.56	951	13-16	1461	27	7.56	35.36	859.69	862.94	1391.27	34.37	1486.87	0.52	0.81
laboratory	Mytilus spp.	4										7.7	14	Extra Feeding	8					-3.25	0.56	16000000	11.80	0.85	7.67	0.13	36.10	3.84	-6.56	951	13-16	1461	27	7.56	35.36	859.69	862.94	1391.27	34.37	1486.87	0.52	0.81
laboratory	Mytilus spp.	5										8.1	16	control	9			-1.34				8000000	13.65	1.03	7.96	0.09	36.58	3.84	-1.57	521	17-20	3490	27	7.85	40.94	1058.12	1062.02	3278.57	170.49	3672.39	2.58	4.02
laboratory	Mytilus spp.	5										8.1	16	control	9				0.56	-1.68	0.28	8000000	13.65	1.03	7.96	0.09	36.58	3.84	-1.57	521	17-20	3490	27	7.85	40.94	1058.12	1062.02	3278.57	170.49	3672.39	2.58	4.02
laboratory	Mytilus spp.	5										8.1	16	control	10			-2.70	0.13	-3.13	0.43	8000000	13.65	1.03	7.96	0.09	36.58	3.84	-1.57	521	17-20	3490	27	7.85	40.94	1058.12	1062.02	3278.57	170.49	3672.39	2.58	4.02
laboratory	Mytilus spp.	5										8.1	16	control	10			-1.94	0.66	2.33	-2.67	8000000	13.65	1.03	7.96	0.09	36.58	3.84	-1.57	521	17-20	3490	27	7.85	40.94	1058.12	1062.02	3278.57	170.49	3672.39	2.58	4.02
laboratory	Mytilus spp.	6										7.7	16	control	11							8000000	13.60	1.03	7.72	0.93	36.10	3.84	-2.67	800	21-24	1281	27	7.61	26.64	685.64	688.17	1218.45	35.90	1321.51	0.54	0.85
laboratory	Mytilus spp.	6										7.7	16	control	11			-2.44	0.62	-2.68	0.48	8000000	13.60	1.03	7.72	0.93	36.10	3.84	-2.67	800	21-24	1281	27	7.61	26.64	685.64	688.17	1218.45	35.90	1321.51	0.54	0.85
laboratory	Mytilus spp.	6										7.7	16	control	12			-0.67	0.39	-3.76	0.74	8000000	13.60	1.03	7.72	0.93	36.10	3.84	-2.67	800	21-24	1281	27	7.61	26.64	685.64	688.17	1218.45	35.90	1321.51	0.54	0.85
laboratory	Mytilus spp.	6										7.7	16	control	12			-1.75	0.55	-4.49	0.79	8000000	13.60	1.03	7.72	0.93	36.10	3.84	-2.67	800	21-24	1281	27	7.61	26.64	685.64	688.17	1218.45	35.90	1321.51	0.54	0.85
laboratory	Mytilus spp.	7										8.1	16	Extra Feeding	13			-1.10	1.21	-2.56	-0.51	16000000	13.55	1.07	7.81	0.11	36.58	3.84	-3.77	584	28-32	2500	27	7.70	41.93	1080.20	1084.19	2371.12	86.95	2583.61	1.32	2.05
laboratory	Mytilus spp.	7										8.1	16	Extra Feeding	13			-2.58		-3.80	0.77	16000000	13.55	1.07	7.81	0.11	36.58	3.84	-3.77	584	28-32	2500	27	7.70	41.93	1080.20	1084.19	2371.12	86.95	2583.61	1.32	2.05
laboratory	Mytilus spp.	7										8.1	16	Extra Feeding	14			-3.96	1.42	-1.53	0.33	16000000	13.55	1.07	7.81	0.11	36.58	3.84	-3.77	584	28-32	2500	27	7.70	41.93	1080.20	1084.19	2371.12	86.95	2583.61	1.32	2.05
laboratory	Mytilus spp.	7										8.1	16	Extra Feeding	14				0.51	-2.50	0.20	16000000	13.55	1.07	7.81	0.11	36.58	3.84	-3.77	584	28-32	2500	27	7.70	41.93	1080.20	1084.19	2371.12	86.95	2583.61	1.32	2.05
laboratory	Mytilus spp.	8										7.7	16	Extra Feeding	15			-1.32	0.35	-4.55	-0.14	16000000	13.70	1.02	7.73	0.13	36.10	3.84	-5.97	821	33-36	1467	27	7.62	29.75	767.95	770.78	1395.04	42.21	1511.47	0.64	1.00
laboratory	Mytilus spp.	8										7.7	16	Extra Feeding	15			-4.19	0.28	-3.65	0.90	16000000	13.70	1.02	7.73	0.13	36.10	3.84	-5.97	821	33-36	1467	27	7.62	29.75	767.95	770.78	1395.04	42.21	1511.47	0.64	1.00
laboratory	Mytilus spp.	8										7.7	16	Extra Feeding	16			-1.85	0.83	-3.81	0.20	16000000	13.70	1.02	7.73	0.13	36.10	3.84	-5.97	821	33-36	1467	27	7.62	29.75	767.95	770.78	1395.04	42.21	1511.47	0.64	1.00
laboratory	Mytilus spp.	8										7.7	16	Extra Feeding	16			-0.68		-2.18	-0.56	16000000	13.70	1.02	7.73	0.13	36.10	3.84	-5.97	821	33-36	1467	27	7.62	29.75	767.95	770.78	1395.04	42.21	1511.47	0.64	1.00