Citation:

PANGAEA.960034

Name: Temperature effects on the physiology of photosynthesis and respiration in Phaeodactylum tricornutum
Published: 2023-07-17
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Diatom; Laboratory experiment; Phaeodactylum tricornutum; Photosynthesis; Physiological performance; Phytoplankton; Respiration; Temperature

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Abstract:

Phaeodactylum tricornutum strain CCAP 1052/1A was cultivated at 6°C and 15°C under controlled conditions (32 salinity, F/2 medium, 400 µatm pCO2, 100 µmol photons m-2 s-2 light intentsity 16:8 light:dark cycle) in semi-continous batch cultures. We assessed the carbonate chemistry (pH, total alkalinity, dissolved inorganic carbon), growth rates, particulate organic carbon and nitrogen (POC and PON), chlorophyll a quota (Chl a), POC:PON ratios, Chl a:POC ratios as well as production rates at both acclimation temperatures. Additionally, we performed biological invivo assays to measure rates of gross photosynthetic oxygen release, gross photosynthetic carbon uptake, respiratory oxygen uptake and respiratory carbon release using membrane-inlet mass-spectrometry. Assays were performed in photosynthesis-irradiance-(PI-)curves of increasing light intensity (0, 50, 150, 250, 400 µmol photons m-2 s-2). First rates were measured under acclimation temperature (6°C and 15°C), directly afterwards, the assay temperature was abruptly shifted to 15°C or 6°C, respectively, and the PI-curve measurement was repeated, so that 6°C acclimated cells were measured at 15°C and 15°C acclimated cells were measured at 6°C. Q10 factors were calculated from acclimated cells und the respective temperature shift. Photosynthetic and respiratory quotients were calculated for acclimated cells as well as after the abrupt temperature shift. PI-parameters, i.e. maximum photosynthesis rate, light use efficiency and light saturation index were calculated. All experiments were performed in laboratories at the Alfred-Wegener-Institute Bremerhaven.

Keyword(s):

Diatom; Laboratory experiment; Phaeodactylum tricornutum; Photosynthesis; Physiological performance; Phytoplankton; Respiration; Temperature

Supplement to:

Rehder, Linda; Rost, Björn; Rokitta, Sebastian D (2023): Abrupt and acclimation responses to changing temperature elicit divergent physiological effects in the diatom Phaeodactylum tricornutum. New Phytologist, 239(3), 1005-1013, https://doi.org/10.1111/nph.18982

References:

Guillard, R R L; Ryther, J H (1962): Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt, and Detonula confervacea (cleve) Gran. Canadian Journal of Microbiology, 8(2), 229-239, https://doi.org/10.1139/m62-029

Knap, Anthony H; Michaels, A; Close, A R; Ducklow, Hugh W; Dickson, Andrew G (1996): Protocols for the Joint Global Ocean Flux Study (JGOFS) Core Measurements. JGOFS, Reprint of the IOC Manuals and Guides No. 29, UNESCO 1994, 19, 210 pp, hdl:10013/epic.27912.d001

Rokitta, Sebastian D; Rost, Björn (2012): Effects of CO2 and their modulation by light in the life-cycle stages of the coccolithophore Emiliania huxleyi. Limnology and Oceanography, 57(2), 607-618, https://doi.org/10.4319/lo.2012.57.2.0607

Additional metadata:

Rehder, Linda (2023): Detailed parameter information for Phaeodactylum tricornutum data. Pangaea_Parameter_Rehder_et_al.csv

Comment:

Phaeodactylum tricornutum strain CCAP 1052/1A was originally sampled in 2003 from an estuary, polluted water (industrial area and seaside resort) Blackpool, England (detailed information from Culture Collection of algae and protozoa; https://www.ccap.ac.uk/catalogue/strain-1055-1). Prior to the experiments, the culture was at least one year cultivated at 15°C in North Sea water enriched with F/2 medium under 16:8 light:dark irradiance of ~10 µmol photons m-2 s-1 with regular dilution every ~4 weeks.

Experiment 1A and 1B refer to 'bulk' parameter measurements, i.e. growth rates, biomass and pigmentation. Due to logistical issues, Exp_1A and Exp_1B are from different batches, but the same stock culture. Exp_2 is the same batch as Exp_1A and refers to physiological measurements. Temperature shifts only apply to Exp_2 and took place after cultures were concentrated and physiological rates were measured under in-situ temperature at four different light levels in the membrane inlet mass spectrometer (MIMS). The temperature shift took place inside the cuvette of the MIMS and took ~1 h.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Experiment	Exp		Rehder, Linda
2	Date/time start, experiment	Date/time start exp		Rehder, Linda		including acclimation time for Exp_1A
3	Date/time end, experiment	Date/time end exp		Rehder, Linda
4	Sampling date/time, experiment	Date/time sampling exp		Rehder, Linda
5	Sample ID	Sample ID		Rehder, Linda
6	Type of study	Study type		Rehder, Linda
7	Laboratory	Lab		Rehder, Linda
8	Species	Species		Rehder, Linda
9	Species, unique identification	Species UID		Rehder, Linda
10	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		Rehder, Linda
11	Species, unique identification (URI)	Species UID (URI)		Rehder, Linda
12	Strain	Strain		Rehder, Linda
13	Treatment: temperature	T:temp	°C	Rehder, Linda
14	Treatment: light:dark cycle	T:L:D	hh:hh	Rehder, Linda
15	Treatment: light intensity	T:Io	µmol/m²/s	Rehder, Linda
16	Salinity	Sal		Rehder, Linda
17	Medium	Medium		Rehder, Linda
18	Generation	Generation	#	Rehder, Linda		in acclimation
19	pH	pH		Rehder, Linda		NBS scale
20	Alkalinity, total	AT	µmol/kg	Rehder, Linda	Titration analyzer, Schott Instruments, TitroLine alpha plus
21	Carbon, inorganic, dissolved	DIC	µmol/kg	Rehder, Linda	Measured with colorimetric assay on QuAAtro continuous segmented flow analyzer (Seal Analytical)
22	Growth rate	µ	1/day	Rehder, Linda	Coulter Counter (Beckman Coulter)
23	Carbon, organic, particulate, per cell	POC/cell	pg/#	Rehder, Linda	Elemental analyzer, EuroVector, EA 3000
24	Nitrogen, organic, particulate, per cell	PON/cell	pg/#	Rehder, Linda	Elemental analyzer, EuroVector, EA 3000
25	Carbon, organic, particulate/Nitrogen, organic, particulate ratio	POC/PON	mol/mol	Rehder, Linda	Calculated
26	Particulate organic carbon production per cell	POC prod/cell	pg/#/day	Rehder, Linda	Calculated	µ*POC
27	Chlorophyll a per cell	Chl a/cell	pg/#	Rehder, Linda	Laboratory fluorometer, Turner, Trilogy
28	Chlorophyll a/particulate organic carbon ratio	Chl a/POC		Rehder, Linda	Calculated	pg/pg
29	Respiratory oxygen uptake rate, per chlorophyll a	RO2	µmol/mg/h	Rehder, Linda	Membrane inlet mass spectrometer (MIMS), GV Instruments, Isoprime	RO2_accl: rate of respiratory O2 uptake under acclimation temperature
30	Respiratory oxygen uptake rate, per chlorophyll a	RO2	µmol/mg/h	Rehder, Linda	Membrane inlet mass spectrometer (MIMS), GV Instruments, Isoprime	RO2_shifted: rate of respiratory O2 uptake after abrupt temperature shift
31	Factor quantifying temperature dependent change of rates of processes	Q10		Rehder, Linda	Calculation according to Rehder et al. (2023)	Q10 of RO2, calculated from RO2_accl and RO2_shifted
32	Respiratory carbon release rate, per chlorophyll a	RCO2	µmol/mg/h	Rehder, Linda	Membrane inlet mass spectrometer (MIMS), GV Instruments, Isoprime	RCO2_accl: rate of respiratory CO2 release under acclimation temperature
33	Respiratory carbon release rate, per chlorophyll a	RCO2	µmol/mg/h	Rehder, Linda	Membrane inlet mass spectrometer (MIMS), GV Instruments, Isoprime	RCO2_shifted: rate of respiratory CO2 release after abrupt temperature shift
34	Factor quantifying temperature dependent change of rates of processes	Q10		Rehder, Linda	Calculation according to Rehder et al. (2023)	Q10 of RO2, calculated from RO2_accl and RO2_shifted
35	Respiratory quotient	RQ		Rehder, Linda	Calculation according to Rehder et al. (2023)	after acclimation
36	Respiratory quotient	RQ		Rehder, Linda	Calculation according to Rehder et al. (2023)	after the abrupt temperature shift
37	Gross photosynthetic oxygen release rate, per chlorophyll a	PSO2	µmol/mg/h	Rehder, Linda	Membrane inlet mass spectrometer (MIMS), GV Instruments, Isoprime	PSO2_accl_V150: rate of photosynthetic O2 release under acclimation temperature at in-situ light intensity
38	Gross photosynthetic oxygen release rate, per chlorophyll a	PSO2	µmol/mg/h	Rehder, Linda	Membrane inlet mass spectrometer (MIMS), GV Instruments, Isoprime	PSO2_shifted_V150: rate of photosynthetic O2 release after abrupt temperature shift at in-situ light intensity
39	Factor quantifying temperature dependent change of rates of processes	Q10		Rehder, Linda	Calculation according to Rehder et al. (2023)	Q10 of PSO2 at in-situ light intensity, calculated from PSO2_accl_V150 and PSO2_shifted_V150
40	Gross photosynthetic carbon uptake rate, per chlorophyll a	PSCO2	µmol/mg/h	Rehder, Linda	Membrane inlet mass spectrometer (MIMS), GV Instruments, Isoprime	PSCO2_accl_V150: rate of photosynthetic C uptake under acclimation temperature at in-situ light intensity
41	Gross photosynthetic carbon uptake rate, per chlorophyll a	PSCO2	µmol/mg/h	Rehder, Linda	Membrane inlet mass spectrometer (MIMS), GV Instruments, Isoprime	PSCO2_shifted_V150: rate of photosynthetic C uptake after abrupt temperature shift at in-situ light intensity
42	Factor quantifying temperature dependent change of rates of processes	Q10		Rehder, Linda	Calculation according to Rehder et al. (2023)	Q10 of PSO2 at in-situ light intensity, calculated from PSO2_accl_V150 and PSO2_shifted_V150
43	Photosynthetic quotient	PQ		Rehder, Linda	Calculation according to Rehder et al. (2023)	under acclimation temperature at in-situ light intensity
44	Photosynthetic quotient	PQ		Rehder, Linda	Calculation according to Rehder et al. (2023)	after abrupt temperature shift at in-situ light intensity
45	Maximum photosynthetic oxygen release rate, per chlorophyll a	PSO2 Vmax	µmol/mg/h	Rehder, Linda	Membrane inlet mass spectrometer (MIMS), GV Instruments, Isoprime	PSO2_accl_Vmax: maximum rate of photosynthetic O2 release under acclimation temperature
46	Maximum photosynthetic oxygen release rate, per chlorophyll a	PSO2 Vmax	µmol/mg/h	Rehder, Linda	Membrane inlet mass spectrometer (MIMS), GV Instruments, Isoprime	PSO2_shifted_Vmax: maximum rate of photosynthetic O2 releaseafter abrupt temperature shift
47	Factor quantifying temperature dependent change of rates of processes	Q10		Rehder, Linda	Calculation according to Rehder et al. (2023)	Q10 of maximum PSO2, calculated PSO2_accl_Vmax and PSO2_shifted_Vmax
48	Maximum photosynthetic carbon uptake rate, per chlorophyll a	PSCO2 Vmax	µmol/mg/h	Rehder, Linda	Membrane inlet mass spectrometer (MIMS), GV Instruments, Isoprime	PSCO2_accl_Vmax: maximum rate of photosynthetic C uptake under acclimation temperature
49	Maximum photosynthetic carbon uptake rate, per chlorophyll a	PSCO2 Vmax	µmol/mg/h	Rehder, Linda	Membrane inlet mass spectrometer (MIMS), GV Instruments, Isoprime	PSCO2_shifted_Vmax: maximum rate of photosynthetic C uptake after abrupt temperature shift
50	Factor quantifying temperature dependent change of rates of processes	Q10		Rehder, Linda	Calculation according to Rehder et al. (2023)	Q10 of maximum PSCO2, calculated PSCO2_accl_Vmax and PSCO2_shifted_Vmax
51	Light saturation index	Ik	µmol/m²/s	Rehder, Linda	Calculation according to Rokitta & Rost (2012)	Ik of PSO2 under acclimation temperature
52	Light saturation index	Ik	µmol/m²/s	Rehder, Linda	Calculation according to Rokitta & Rost (2012)	Ik of PSO2 after abrupt temperature shift
53	Light use efficiency	alpha	µmol/m²/s	Rehder, Linda	Calculation according to Rokitta & Rost (2012)	alpha of PSO2 under acclimation temperature
54	Light use efficiency	alpha	µmol/m²/s	Rehder, Linda	Calculation according to Rokitta & Rost (2012)	alpha of PSO2 after abrupt temperature shift
55	Light saturation index	Ik	µmol/m²/s	Rehder, Linda	Calculation according to Rokitta & Rost (2012)	Ik of PSCO2 under acclimation temperature
56	Light saturation index	Ik	µmol/m²/s	Rehder, Linda	Calculation according to Rokitta & Rost (2012)	Ik of PSCO2 after abrupt temperature shift
57	Light use efficiency	alpha	µmol/m²/s	Rehder, Linda	Calculation according to Rokitta & Rost (2012)	alpha of PSCO2 under acclimation temperature
58	Light use efficiency	alpha	µmol/m²/s	Rehder, Linda	Calculation according to Rokitta & Rost (2012)	alpha of PSCO2 after abrupt temperature shift

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

736 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Exp	2 Date/time start exp (including acclimation time fo...)	3 Date/time end exp	4 Date/time sampling exp	5 Sample ID	6 Study type	7 Lab	8 Species	9 Species UID	10 Species UID (Semantic URI)	11 Species UID (URI)	12 Strain	13 T:temp [°C]	14 T:L:D [hh:hh]	15 T:Io [µmol/m²/s]	16 Sal	17 Medium	18 Generation [#] (in acclimation)	19 pH (NBS scale)	20 AT [µmol/kg] (Titration analyzer, Schott In...)	21 DIC [µmol/kg] (Measured with colorimetric as...)	22 µ [1/day] (Coulter Counter (Beckman Coul...)	23 POC/cell [pg/#] (Elemental analyzer, EuroVecto...)	24 PON/cell [pg/#] (Elemental analyzer, EuroVecto...)	25 POC/PON [mol/mol] (Calculated)	26 POC prod/cell [pg/#/day] (µ*POC, Calculated)	27 Chl a/cell [pg/#] (Laboratory fluorometer, Turne...)	28 Chl a/POC (pg/pg, Calculated)	29 RO2 [µmol/mg/h] (RO2_accl: rate of respiratory...)	30 RO2 [µmol/mg/h] (RO2_shifted: rate of respirat...)	31 Q10 (Q10 of RO2, calculated from R...)	32 RCO2 [µmol/mg/h] (RCO2_accl: rate of respirator...)	33 RCO2 [µmol/mg/h] (RCO2_shifted: rate of respira...)	34 Q10 (Q10 of RO2, calculated from R...)	35 RQ (after acclimation, Calculatio...)	36 RQ (after the abrupt temperature ...)	37 PSO2 [µmol/mg/h] (PSO2_accl_V150: rate of photo...)	38 PSO2 [µmol/mg/h] (PSO2_shifted_V150: rate of ph...)	39 Q10 (Q10 of PSO2 at in-situ light ...)	40 PSCO2 [µmol/mg/h] (PSCO2_accl_V150: rate of phot...)	41 PSCO2 [µmol/mg/h] (PSCO2_shifted_V150: rate of p...)	42 Q10 (Q10 of PSO2 at in-situ light ...)	43 PQ (under acclimation temperature...)	44 PQ (after abrupt temperature shif...)	45 PSO2 Vmax [µmol/mg/h] (PSO2_accl_Vmax: maximum rate ...)	46 PSO2 Vmax [µmol/mg/h] (PSO2_shifted_Vmax: maximum ra...)	47 Q10 (Q10 of maximum PSO2, calculat...)	48 PSCO2 Vmax [µmol/mg/h] (PSCO2_accl_Vmax: maximum rate...)	49 PSCO2 Vmax [µmol/mg/h] (PSCO2_shifted_Vmax: maximum r...)	50 Q10 (Q10 of maximum PSCO2, calcula...)	51 Ik [µmol/m²/s] (Ik of PSO2 under acclimation ...)	52 Ik [µmol/m²/s] (Ik of PSO2 after abrupt tempe...)	53 alpha [µmol/m²/s] (alpha of PSO2 under acclimati...)	54 alpha [µmol/m²/s] (alpha of PSO2 after abrupt te...)	55 Ik [µmol/m²/s] (Ik of PSCO2 under acclimation...)	56 Ik [µmol/m²/s] (Ik of PSCO2 after abrupt temp...)	57 alpha [µmol/m²/s] (alpha of PSCO2 under acclimat...)	58 alpha [µmol/m²/s] (alpha of PSCO2 after abrupt t...)
Exp_1A	2020-07-07	2020-07-29	2020-07-29T10:00	6_A1	laboratory experiment	Alfred-Wegener-Institute Bremerhaven, Marine Biogeoscience	Phaeodactylum tricornutum Bohlin CCAP 1052/1A	Phaeodactylum tricornutum	urn:lsid:marinespecies.org:taxname:175584	marinespecies.org	CCAP 1052/1A	6	16:08	100	32	Northsea water, F/2 medium	>10	8.079	2347.561368	2161.886178	0.434256469					0.122773132
Exp_1A	2020-07-07	2020-07-29	2020-07-29T10:00	6_B1	laboratory experiment	Alfred-Wegener-Institute Bremerhaven, Marine Biogeoscience	Phaeodactylum tricornutum Bohlin CCAP 1052/1A	Phaeodactylum tricornutum	urn:lsid:marinespecies.org:taxname:175584	marinespecies.org	CCAP 1052/1A	6	16:08	100	32	Northsea water, F/2 medium	>10	8.094	2340.385416	2111.516122	0.438180039					0.164452919
Exp_1A	2020-07-07	2020-07-29	2020-07-29T10:00	6_C1	laboratory experiment	Alfred-Wegener-Institute Bremerhaven, Marine Biogeoscience	Phaeodactylum tricornutum Bohlin CCAP 1052/1A	Phaeodactylum tricornutum	urn:lsid:marinespecies.org:taxname:175584	marinespecies.org	CCAP 1052/1A	6	16:08	100	32	Northsea water, F/2 medium	>10	8.082	2343.973392	2094.929422	0.414995063					0.140905398
Exp_1A	2020-07-07	2020-07-29	2020-07-29T10:00	6_D1	laboratory experiment	Alfred-Wegener-Institute Bremerhaven, Marine Biogeoscience	Phaeodactylum tricornutum Bohlin CCAP 1052/1A	Phaeodactylum tricornutum	urn:lsid:marinespecies.org:taxname:175584	marinespecies.org	CCAP 1052/1A	6	16:08	100	32	Northsea water, F/2 medium	>10	8.085	2333.209464	2085.139374	0.424286378					0.105174739
Exp_1A	2020-07-07	2020-07-29	2020-07-29T10:00	15_A1	laboratory experiment	Alfred-Wegener-Institute Bremerhaven, Marine Biogeoscience	Phaeodactylum tricornutum Bohlin CCAP 1052/1A	Phaeodactylum tricornutum	urn:lsid:marinespecies.org:taxname:175584	marinespecies.org	CCAP 1052/1A	15	16:08	100	32	Northsea water, F/2 medium	>10	8.172	2440.336173	2075.013593	0.917995964					0.362620761
Exp_1A	2020-07-07	2020-07-29	2020-07-29T10:00	15_B1	laboratory experiment	Alfred-Wegener-Institute Bremerhaven, Marine Biogeoscience	Phaeodactylum tricornutum Bohlin CCAP 1052/1A	Phaeodactylum tricornutum	urn:lsid:marinespecies.org:taxname:175584	marinespecies.org	CCAP 1052/1A	15	16:08	100	32	Northsea water, F/2 medium	>10	8.173	2425.984269	2088.763230	0.975408247					0.390044905
Exp_1A	2020-07-07	2020-07-29	2020-07-29T10:00	15_C1	laboratory experiment	Alfred-Wegener-Institute Bremerhaven, Marine Biogeoscience	Phaeodactylum tricornutum Bohlin CCAP 1052/1A	Phaeodactylum tricornutum	urn:lsid:marinespecies.org:taxname:175584	marinespecies.org	CCAP 1052/1A	15	16:08	100	32	Northsea water, F/2 medium	>10	8.171	2424.959133	2077.994176	0.938755306					0.351797973
Exp_1A	2020-07-07	2020-07-29	2020-07-29T10:00	15_D1	laboratory experiment	Alfred-Wegener-Institute Bremerhaven, Marine Biogeoscience	Phaeodactylum tricornutum Bohlin CCAP 1052/1A	Phaeodactylum tricornutum	urn:lsid:marinespecies.org:taxname:175584	marinespecies.org	CCAP 1052/1A	15	16:08	100	32	Northsea water, F/2 medium	>10	8.125	2425.984269	2109.453036	0.744721411					0.379147313
Exp_1B	2019-12-10	2019-12-17	2019-12-19T10:00	6_A2	laboratory experiment	Alfred-Wegener-Institute Bremerhaven, Marine Biogeoscience	Phaeodactylum tricornutum Bohlin CCAP 1052/1A	Phaeodactylum tricornutum	urn:lsid:marinespecies.org:taxname:175584	marinespecies.org	CCAP 1052/1A	6	16:08	100	32	Northsea water, F/2 medium	>10					15.701369820	2.600169041	5.176584562	6.818421410		0.007819262
Exp_1B	2019-12-10	2019-12-17	2019-12-19T10:00	6_B2	laboratory experiment	Alfred-Wegener-Institute Bremerhaven, Marine Biogeoscience	Phaeodactylum tricornutum Bohlin CCAP 1052/1A	Phaeodactylum tricornutum	urn:lsid:marinespecies.org:taxname:175584	marinespecies.org	CCAP 1052/1A	6	16:08	100	32	Northsea water, F/2 medium	>10					16.832400720	2.475741581	5.828862036	7.375622009		0.009770022
Exp_1B	2019-12-10	2019-12-17	2019-12-19T10:00	6_C2	laboratory experiment	Alfred-Wegener-Institute Bremerhaven, Marine Biogeoscience	Phaeodactylum tricornutum Bohlin CCAP 1052/1A	Phaeodactylum tricornutum	urn:lsid:marinespecies.org:taxname:175584	marinespecies.org	CCAP 1052/1A	6	16:08	100	32	Northsea water, F/2 medium	>10					18.328182480	2.987856890	5.258930823	7.606105246		0.007687909
Exp_1B	2019-12-10	2019-12-17	2019-12-19T10:00	6_D2	laboratory experiment	Alfred-Wegener-Institute Bremerhaven, Marine Biogeoscience	Phaeodactylum tricornutum Bohlin CCAP 1052/1A	Phaeodactylum tricornutum	urn:lsid:marinespecies.org:taxname:175584	marinespecies.org	CCAP 1052/1A	6	16:08	100	32	Northsea water, F/2 medium	>10					16.899477690	2.634542639	5.494126768	7.170218183		0.006223550
Exp_1B	2019-12-10	2019-12-17	2019-12-19T10:00	15_A2	laboratory experiment	Alfred-Wegener-Institute Bremerhaven, Marine Biogeoscience	Phaeodactylum tricornutum Bohlin CCAP 1052/1A	Phaeodactylum tricornutum	urn:lsid:marinespecies.org:taxname:175584	marinespecies.org	CCAP 1052/1A	15	16:08	100	32	Northsea water, F/2 medium	>10					2.600169041	3.911363967	6.349109538	26.598175440		0.012515309
Exp_1B	2019-12-10	2019-12-17	2019-12-19T10:00	15_B2	laboratory experiment	Alfred-Wegener-Institute Bremerhaven, Marine Biogeoscience	Phaeodactylum tricornutum Bohlin CCAP 1052/1A	Phaeodactylum tricornutum	urn:lsid:marinespecies.org:taxname:175584	marinespecies.org	CCAP 1052/1A	15	16:08	100	32	Northsea water, F/2 medium	>10					2.475741581	4.343460114	4.270007879	21.118779500		0.018014915
Exp_1B	2019-12-10	2019-12-17	2019-12-19T10:00	15_C2	laboratory experiment	Alfred-Wegener-Institute Bremerhaven, Marine Biogeoscience	Phaeodactylum tricornutum Bohlin CCAP 1052/1A	Phaeodactylum tricornutum	urn:lsid:marinespecies.org:taxname:175584	marinespecies.org	CCAP 1052/1A	15	16:08	100	32	Northsea water, F/2 medium	>10					2.987856890	4.138654259	4.471871376	20.240821040		0.016316147
Exp_1B	2019-12-10	2019-12-17	2019-12-19T10:00	15_D2	laboratory experiment	Alfred-Wegener-Institute Bremerhaven, Marine Biogeoscience	Phaeodactylum tricornutum Bohlin CCAP 1052/1A	Phaeodactylum tricornutum	urn:lsid:marinespecies.org:taxname:175584	marinespecies.org	CCAP 1052/1A	15	16:08	100	32	Northsea water, F/2 medium	>10					2.634542639	4.074813988	4.262806645	15.268521990		0.018492892
Exp_2	2020-09-11	2020-10-28	2020-09-11T10:00	6_A	laboratory experiment	Alfred-Wegener-Institute Bremerhaven, Marine Biogeoscience	Phaeodactylum tricornutum Bohlin CCAP 1052/1A	Phaeodactylum tricornutum	urn:lsid:marinespecies.org:taxname:175584	marinespecies.org	CCAP 1052/1A	6	16:08	100	32	Northsea water, F/2 medium	>10											32.14330430	50.83687027	1.664214136	68.19915313	40.66005348	0.562901210	2.121721914	0.799814254	191.6902421	291.53846450	1.593414369	137.46391800	254.65619570	1.983888473	1.394476782	1.144831618	73.80000000	135.56688040	1.966615564	54.84634501	110.31872840	2.173821857	66.50000000	91.84919871	1.720000000	1.450130930	72.47592471	118.70458360	0.863650280	0.981268305
Exp_2	2020-09-11	2020-10-28	2020-09-14T10:00	6_B	laboratory experiment	Alfred-Wegener-Institute Bremerhaven, Marine Biogeoscience	Phaeodactylum tricornutum Bohlin CCAP 1052/1A	Phaeodactylum tricornutum	urn:lsid:marinespecies.org:taxname:175584	marinespecies.org	CCAP 1052/1A	6	16:08	100	32	Northsea water, F/2 medium	>10											37.38684354	59.12371346	1.664020917	79.31402872	45.46347926	0.538838365	2.121442229	0.768955071	202.2805638	268.24863410	1.368370610	129.70817400	135.36662970	1.048587666	1.559505137	1.981645216	78.04309727	135.00000000	1.839044491	46.37091494	170.25552370	4.242455280	36.79549159	74.40000000	1.242988348	1.080000000	62.89416393	346.33898970	0.877895578	0.496523683
Exp_2	2020-09-11	2020-10-28	2020-09-17T10:00	6_C	laboratory experiment	Alfred-Wegener-Institute Bremerhaven, Marine Biogeoscience	Phaeodactylum tricornutum Bohlin CCAP 1052/1A	Phaeodactylum tricornutum	urn:lsid:marinespecies.org:taxname:175584	marinespecies.org	CCAP 1052/1A	6	16:08	100	32	Northsea water, F/2 medium	>10											62.93220019	85.38417374	1.403547720	308.99793030	404.03369990				154.8850308	258.54423480	1.767056587						72.00000000	154.10000000	2.328072056				52.90000000	69.30000000	1.550000000	1.070000000
Exp_2	2020-09-11	2020-10-28	2020-10-12T10:00	6_D	laboratory experiment	Alfred-Wegener-Institute Bremerhaven, Marine Biogeoscience	Phaeodactylum tricornutum Bohlin CCAP 1052/1A	Phaeodactylum tricornutum	urn:lsid:marinespecies.org:taxname:175584	marinespecies.org	CCAP 1052/1A	6	16:08	100	32	Northsea water, F/2 medium	>10											30.91381896	55.33224068	1.909489749	54.28051560	43.82733472	0.788458784	1.755865740	0.792075907	165.1438978	232.12243200	1.459764736	92.45315144	150.39227550	1.717046324	1.786244117	1.543446505	71.92495041	135.90000000	2.028410265	35.01192632	75.80504955	2.359162859	47.66469497	70.60000000	0.874452074	1.010000000		113.94542640		0.716230575
Exp_2	2020-09-11	2020-10-28	2020-10-16T10:00	6_E	laboratory experiment	Alfred-Wegener-Institute Bremerhaven, Marine Biogeoscience	Phaeodactylum tricornutum Bohlin CCAP 1052/1A	Phaeodactylum tricornutum	urn:lsid:marinespecies.org:taxname:175584	marinespecies.org	CCAP 1052/1A	6	16:08	100	32	Northsea water, F/2 medium	>10											50.70489031	66.38992969	1.349143605		54.63628482			0.822960426	183.5243066	267.08449790	1.517264200		153.57412210				72.10000000	154.27216930	2.328875230				51.80000000	63.96353422	1.010000000	1.038638002		374.03165200		0.475635895
Exp_2	2020-09-11	2020-10-28	2020-10-20T10:00	15_A	laboratory experiment	Alfred-Wegener-Institute Bremerhaven, Marine Biogeoscience	Phaeodactylum tricornutum Bohlin CCAP 1052/1A	Phaeodactylum tricornutum	urn:lsid:marinespecies.org:taxname:175584	marinespecies.org	CCAP 1052/1A	15	16:08	100	32	Northsea water, F/2 medium	>10											44.12381930	16.60115432	0.337516225	27.69625853	58.59054024	2.299125427	3.529305198	0.627694043	220.2909656	99.11481975	0.411720149	177.18673140	84.34118693	0.438315910	1.243270102	1.175165105	97.23276354	42.10000000	0.394142220	65.79585613	32.22189819	0.452379447	91.84919871	66.50000000	1.116330846	0.660000000	64.53737909	19.82469754	1.072366887	1.947172516
Exp_2	2020-09-11	2020-10-28	2020-10-22T10:00	15_B	laboratory experiment	Alfred-Wegener-Institute Bremerhaven, Marine Biogeoscience	Phaeodactylum tricornutum Bohlin CCAP 1052/1A	Phaeodactylum tricornutum	urn:lsid:marinespecies.org:taxname:175584	marinespecies.org	CCAP 1052/1A	15	16:08	100	32	Northsea water, F/2 medium	>10											38.66583698	15.54767254	0.363392134	35.35002808	34.41859717	0.970766747	2.213745953	0.914244481	176.8287300	96.86992076	0.512384148	99.24955744	73.97545453	0.721400995	1.781657617	1.309487334	77.10000000	36.78558329	0.439337478	47.70098628	32.53348783	0.653637504	74.40000000	36.79549159	1.100000000	1.049109833	72.94925389	42.52123506	0.704241697	0.855472605
Exp_2	2020-09-11	2020-10-28	2020-10-26T10:00	15_C	laboratory experiment	Alfred-Wegener-Institute Bremerhaven, Marine Biogeoscience	Phaeodactylum tricornutum Bohlin CCAP 1052/1A	Phaeodactylum tricornutum	urn:lsid:marinespecies.org:taxname:175584	marinespecies.org		15	16:08	100	32	Northsea water, F/2 medium	>10											39.42999288	16.56071202	0.381409452	44.89679473	74.01892757	1.742820758	4.469549830	1.138645773	246.2442187	92.67492891	0.337629389	164.25256590	158.53307620	0.961385287	1.499180346	0.584577876	96.00000000	37.10000000	0.348123802	61.51175117	57.27850848	0.923831783	69.30000000	52.90000000	1.450000000	0.740000000	38.04316567	19.26977748	1.752730281	3.402394072
Exp_2	2020-09-11	2020-10-28	2020-10-27T10:00	15_D	laboratory experiment	Alfred-Wegener-Institute Bremerhaven, Marine Biogeoscience	Phaeodactylum tricornutum Bohlin CCAP 1052/1A	Phaeodactylum tricornutum	urn:lsid:marinespecies.org:taxname:175584	marinespecies.org		15	16:08	100	32	Northsea water, F/2 medium	>10											37.50483829	24.33688454	0.618455617						218.8241762	127.19305290	0.547251255						88.40000000	47.42129157	0.500695620	49.11498894			70.60000000	47.66469497	1.310000000	1.054732336	37.58708713		1.588173745
Exp_2	2020-09-11	2020-10-28	2020-10-28T10:00	15_E	laboratory experiment	Alfred-Wegener-Institute Bremerhaven, Marine Biogeoscience	Phaeodactylum tricornutum Bohlin CCAP 1052/1A	Phaeodactylum tricornutum	urn:lsid:marinespecies.org:taxname:175584	marinespecies.org		15	16:08	100	32	Northsea water, F/2 medium	>10											17.76661063	14.81641423	0.817289917						115.0354516	81.70492102	0.683765582						45.06747516	32.70000000	0.700984677	50.84923846			63.96353422	51.80000000	0.737339533	0.660000000	55.82775549		1.048052045