Armstrong, Evelyn; Law, Cliff S (2023): Cell volume of Emiliania huxleyi measured at cross-over points during laboratory experiments [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.959827, In: Armstrong, E; Law, CS (2023): Physiological measurements from the long-term laboratory incubation of Emiliania huxleyi grown at present day temperature and pH conditions in subantarctic waters and projected conditions for 2100 [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.959857
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Abstract:
On four occasions during the two year incubation of a subantarctic E. huxleyi culture (2015-11-20 to 2017-12-01), from Day 309 onwards, cell size of the cultures was measured at cross-over points, after 7 – 8 generations while still in exponential growth phase. Cells from Now (11°C and pH 8.1) and Future (14°C and pH 7.8) cultures, and also the crossover experiments - Future cells inoculated into Now medium and incubated under Now conditions (Future in Now), and Now cells incubated in Future medium under Future conditions (Now in Future) were fixed in 1 % glutaraldehyde. Subsamples were mounted in a nanoplankton chamber (Phytotech) and measured with a calibrated eye-piece graticule under an Olympus IX70 inverted microscope. As the fixation process caused coccoliths to separate, the cell size measurements were for the cell body only, which allows more accurate determinations of cell volume (Buitenhuis et al., 2008). Cell volume was calculated assuming that the cells were spherical.
Keyword(s):
Supplement to:
Armstrong, Evelyn; Law, Cliff S (2023): Resilience of Emiliania huxleyi to future changes in subantarctic waters. PLoS ONE, 18(11), e0284415, https://doi.org/10.1371/journal.pone.0284415
References:
Buitenhuis, Erik Theodoor; Pangerc, Tanja; Franklin, Daniel J; Le Quéré, Corinne; Malin, Gill (2008): Growth rates of six coccolithophorid strains as a function of temperature. Limnology and Oceanography, 53(3), 1181-1185, https://doi.org/10.4319/lo.2008.53.3.1181
Funding:
Coverage:
Latitude: -45.829000 * Longitude: 171.532000
Date/Time Start: 2014-06-03T00:00:00 * Date/Time End: 2014-06-03T00:00:00
Event(s):
Emiliania_huxleyi_subantarctic * Latitude: -45.829000 * Longitude: 171.532000 * Date/Time: 2014-06-03T00:00:00 * Campaign: P140603 * Basis: Polaris II * Method/Device: Bottle, Niskin (NIS) * Comment: Original sampling of E. huxleyi between 2 -5 m depth
Comment:
The study was supported by Coasts and Oceans Centre (Strategic Science Investment Fund of the National Institute of Water and Atmospheric Research).
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Species, unique identification | Species UID | Armstrong, Evelyn | |||
| 2 | Species, unique identification (Semantic URI) | Species UID (Semantic URI) | Armstrong, Evelyn | |||
| 3 | Species, unique identification (URI) | Species UID (URI) | Armstrong, Evelyn | |||
| 4 | Type of study | Study type | Armstrong, Evelyn | |||
| 5 | Comment | Comment | Armstrong, Evelyn | |||
| 6 | Treatment | Treat | Armstrong, Evelyn | |||
| 7 | Time in days | Time | days | Armstrong, Evelyn | 2015-11-20, experiment, start. 2017-12-01, experiment, finish. | |
| 8 | Emiliania huxleyi, cell biovolume | E. huxleyi cell biovol | µm3 | Armstrong, Evelyn | Inverted tissue culture microscope, Olympus, IX70 |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
285 data points