Bodur, Yasemin V; Felden, Janine; Braeckman, Ulrike (2024): Single prokaryotic cell abundances of the Northeast Greenland (NEG) shelf sediments from POLARSTERN cruise PS109 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.959552
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Abstract:
Samples for single cell abundances as an estimate for the abundance of benthic bacteria on the Northeast Greenland shelf have been collected with R/V Polarstern during PS109 between September and October 2017 using a camera-equipped multiple corer (TV-MUC; core area 0.007 m²). Upon arrival on deck, the upper 5 cm of three MUC cores were sliced into 1 cm layers with a 10 ml cut-off syringe. 2 ml of each slice was transferred into a scintillation vial and fixed with 2 % filtered formaldehyde-seawater solution. In the laboratory, the samples were diluted, filtered through polycarbonate filters (0.2 µm, Whatman Nucleopore Track-Etch Membrane) and stained with a 0.001 % acridine orange solution after Hobbie et al., 1977 (doi:10.1128/aem.33.5.1225-1228.1977). Cells were counted on at least 30 grids (125 µm * 125 µm area) for 2 replicate filters per sample each with a Zeiss Axiophot microscope (Germany) and a 100x oil immersion objective lens (Zeiss Plan-Apochromat, Germany).
Supplement to:
Bodur, Yasemin V; Renaud, Paul E; Lins, Lidia; Da Costa Monteiro, Luana; Ambrose, William G Jr; Felden, Janine; Krumpen, Thomas; Wenzhöfer, Frank; Wlodarska-Kowalczuk, Maria; Braeckman, Ulrike (submitted): Weaker pelagic-benthic coupling leads to ecosystem change on an Arctic outflow shelf (Northeast Greenland). Elementa - Science of the Anthropocene
Project(s):
Funding:
Coverage:
Median Latitude: 78.720554 * Median Longitude: -14.878132 * South-bound Latitude: 76.486900 * West-bound Longitude: -19.321920 * North-bound Latitude: 80.317630 * East-bound Longitude: -7.132010
Date/Time Start: 2017-09-17T15:16:00 * Date/Time End: 2017-10-07T02:06:00
Minimum DEPTH, sediment/rock: 0.005 m * Maximum DEPTH, sediment/rock: 0.045 m
Event(s):
PS109_19-4 * Latitude: 80.134150 * Longitude: -7.931730 * Date/Time: 2017-09-17T15:16:00 * Elevation: -315.0 m * Campaign: PS109 (ARK-XXXI/4) * Basis: Polarstern * Method/Device: Multicorer with television (TVMUC)
PS109_36-2 * Latitude: 80.317630 * Longitude: -10.031730 * Date/Time: 2017-09-19T06:09:00 * Elevation: -318.0 m * Campaign: PS109 (ARK-XXXI/4) * Basis: Polarstern * Method/Device: Multicorer with television (TVMUC)
PS109_45-4 * Latitude: 80.134370 * Longitude: -17.710500 * Date/Time: 2017-09-20T20:05:00 * Elevation: -219.0 m * Campaign: PS109 (ARK-XXXI/4) * Basis: Polarstern * Method/Device: Multicorer with television (TVMUC)
Comment:
Single cell abundances were calculated with the following equation:
single cell abundance (cell number ml-1 sediment-1) = average cell count * (filtration funnel area/grid area) * dilution
where
filtration funnel area = 203077576 µm²
grid area = 15625 µm²
dilution=4830 µl
Parameter(s):
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1928 data points