Citation:

PANGAEA.958998

Name: Seawater carbonate chemistry and photosystem II (PSII) function, bleaching of coralline alga Amphiroa gracilis and larval settlement ofthe sea urchin Heliocidaris erythrogramma
Published: 2023-05-16
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Amphiroa gracilis; Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Community composition and diversity; Echinodermata; Entire community; Heliocidaris erythrogramma; Indian Ocean; Laboratory experiment; Macroalgae; Other studied parameter or process; Plantae; Primary production/Photosynthesis; Reproduction; Rhodophyta; Single species; Soft-bottom community; Species interaction; Temperate; Temperature

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Abstract:

Coralline algae are a crucial component of reef systems, stabilising reef substrate, providing habitat and contributing to accretion. Coralline algae and their surface microbial biofilms are also important as settlement cues for marine invertebrates, yet few studies address the impact of future environmental conditions on interactions between coralline algae, reef microbes and settlement by larvae of marine invertebrates. We exposed the temperate coralline algal species Amphiroa gracilis to warming and/or acidification scenarios for 21 days. Algae became bleached but photosystem II (PSII) function was not measurably impacted. Settlement by larvae of the sea urchin Heliocidaris erythrogramma was reduced and the structure of the prokaryotic community associated with A. gracilis was altered. Coralline algae in ambient conditions were dominated by Alphaproteobacteria from the Rhodobacteraceae including Loktonella; those under warming were dominated by Bacteroidetes and Verrucomicrobia; acidification resulted in less Loktonella and more Planctomycetes; and a combination of warming and acidification caused increases in Bacteroidetes, Verrucomicrobia and the Alphaproteobacteria family Hyphomonadaceae. These experiments indicate that predicted future environmental change may reduce the ability of some temperate reef coralline algae and associated reef microbes to facilitate settlement of invertebrate larvae as well as having a direct impact to algae via bleaching.

Keyword(s):

Amphiroa gracilis; Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Community composition and diversity; Echinodermata; Entire community; Heliocidaris erythrogramma; Indian Ocean; Laboratory experiment; Macroalgae; Other studied parameter or process; Plantae; Primary production/Photosynthesis; Reproduction; Rhodophyta; Single species; Soft-bottom community; Species interaction; Temperate; Temperature

Supplement to:

Huggett, Megan J; McMahon, Kathryn; Bernasconi, Rachele (2018): Future warming and acidification result in multiple ecological impacts to a temperate coralline alga. Environmental Microbiology, 20(8), 2769-2782, https://doi.org/10.1111/1462-2920.14113

Documentation:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2022): seacarb: seawater carbonate chemistry with R. R package version 3.3.1. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: -31.685600 * Longitude: 115.698300

Date/Time Start: 2013-11-25T00:00:00 * Date/Time End: 2013-11-25T00:00:00

Event(s):

Mindarie_Keys * Latitude: -31.685600 * Longitude: 115.698300 * Date/Time: 2013-11-25T00:00:00 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-05-15.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type of study	Study type		Huggett, Megan J
2	Species	Species		Huggett, Megan J
3	Time point, descriptive	Time point		Huggett, Megan J
4	Treatment	Treat		Huggett, Megan J
5	Maximal electron transport rate, relative	rETR max		Huggett, Megan J
6	Light saturation	Ek	µmol/m²/s	Huggett, Megan J
7	Identification	ID		Huggett, Megan J		Tank
8	Percentage	Perc	%	Huggett, Megan J		Healthy tissue
9	Percentage	Perc	%	Huggett, Megan J		Faded tissue
10	Percentage	Perc	%	Huggett, Megan J		Bleached tissue
11	Percentage	Perc	%	Huggett, Megan J		impacted tissues
12	Identification	ID		Huggett, Megan J		Treatment
13	Metamorphosed, larvae	Metamorph larv	%	Huggett, Megan J
14	Temperature, water	Temp	°C	Huggett, Megan J
15	Temperature, water, standard error	T std e	±	Huggett, Megan J
16	pH	pH		Huggett, Megan J		total scale
17	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Huggett, Megan J	Calculated using CO2SYS
18	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error	pCO2water_SST_wet std e	±	Huggett, Megan J	Calculated using CO2SYS
19	Alkalinity, total	AT	µmol/kg	Huggett, Megan J	Potentiometric titration
20	Alkalinity, total, standard error	AT std e	±	Huggett, Megan J	Potentiometric titration
21	Salinity	Sal		Huggett, Megan J
22	Salinity, standard error	Sal std e	±	Huggett, Megan J
23	Calcite saturation state	Omega Cal		Huggett, Megan J
24	Calcite saturation state, standard error	Omega Cal std e	±	Huggett, Megan J
25	Aragonite saturation state	Omega Arg		Huggett, Megan J
26	Aragonite saturation state, standard error	Omega Arg std e	±	Huggett, Megan J
27	Sample number	Sample no		Huggett, Megan J
28	Operational taxonomic unit	OTU	#	Huggett, Megan J
29	Operational taxonomic unit, standard deviation	OTU std dev	±	Huggett, Megan J
30	Chao 1 richness	Chao1 rich		Huggett, Megan J
31	Chao 1 richness, standard deviation	Chao 1 rich std dev	±	Huggett, Megan J
32	Pielou evenness index	J'		Huggett, Megan J
33	Pielou evenness index, standard deviation	J' std dev	±	Huggett, Megan J
34	Shannon Diversity Index	H'		Huggett, Megan J
35	Shannon Diversity Index, standard deviation	H' std dev	±	Huggett, Megan J
36	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
40	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

7584 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Study type	2 Species	3 Time point	4 Treat	5 rETR max	6 Ek [µmol/m²/s]	7 ID (Tank)	8 Perc [%] (Healthy tissue)	9 Perc [%] (Faded tissue)	10 Perc [%] (Bleached tissue)	11 Perc [%] (impacted tissues)	12 ID (Treatment)	13 Metamorph larv [%]	14 Temp [°C]	15 T std e [±]	16 pH (total scale)	17 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	18 pCO2water_SST_wet std e [±] (Calculated using CO2SYS)	19 AT [µmol/kg] (Potentiometric titration)	20 AT std e [±] (Potentiometric titration)	21 Sal	22 Sal std e [±]	23 Omega Cal	24 Omega Cal std e [±]	25 Omega Arg	26 Omega Arg std e [±]	27 Sample no	28 OTU [#]	29 OTU std dev [±]	30 Chao1 rich	31 Chao 1 rich std dev [±]	32 J'	33 J' std dev [±]	34 H'	35 H' std dev [±]	36 CSC flag (Calculated using seacarb afte...)	37 CO2 [µmol/kg] (Calculated using seacarb afte...)	38 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	39 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	40 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	41 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	42 DIC [µmol/kg] (Calculated using seacarb afte...)	43 Omega Arg (Calculated using seacarb afte...)	44 Omega Cal (Calculated using seacarb afte...)
laboratory	Amphiroa gracilis (macroalga)	initial	Ambient	2.903	30.782
laboratory	Amphiroa gracilis (macroalga)	initial	Ambient	2.961	26.182
laboratory	Amphiroa gracilis (macroalga)	initial	Ambient	2.568	21.489
laboratory	Amphiroa gracilis (macroalga)	initial	Ambient	2.688	25.720
laboratory	Amphiroa gracilis (macroalga)	initial	Ambient	2.461	17.198
laboratory	Amphiroa gracilis (macroalga)	initial	Ambient	2.538	24.664
laboratory	Amphiroa gracilis (macroalga)	initial	Ambient	3.768	29.394
laboratory	Amphiroa gracilis (macroalga)	initial	Ambient	3.171	27.552
laboratory	Amphiroa gracilis (macroalga)	initial	Ambient	2.776	20.232
laboratory	Amphiroa gracilis (macroalga)	initial	Ambient	2.708	26.090
laboratory	Amphiroa gracilis (macroalga)	initial	Ambient	3.206	26.715
laboratory	Amphiroa gracilis (macroalga)	initial	Ambient	3.245	29.289
laboratory	Amphiroa gracilis (macroalga)	initial	Ambient	3.444	27.441
laboratory	Amphiroa gracilis (macroalga)	initial	Ambient	3.437	29.057
laboratory	Amphiroa gracilis (macroalga)	1 week	Ambient	1.288	11.476								19.0	0.03	8.1	379	10	2327	6	37.4	0.02	4.6	0.09	3.0	0.06										8	11.24	341.62	342.80	1809.83	207.97	2029.03	3.17	4.88
laboratory	Amphiroa gracilis (macroalga)	1 week	Ambient	2.304	23.611								19.0	0.03	8.1	379	10	2327	6	37.4	0.02	4.6	0.09	3.0	0.06										8	11.24	341.62	342.80	1809.83	207.97	2029.03	3.17	4.88
laboratory	Amphiroa gracilis (macroalga)	1 week	Ambient	1.796	17.543								19.0	0.03	8.1	379	10	2327	6	37.4	0.02	4.6	0.09	3.0	0.06										8	11.24	341.62	342.80	1809.83	207.97	2029.03	3.17	4.88
laboratory	Amphiroa gracilis (macroalga)	1 week	Ambient	1.796	17.543								19.0	0.03	8.1	379	10	2327	6	37.4	0.02	4.6	0.09	3.0	0.06										8	11.24	341.62	342.80	1809.83	207.97	2029.03	3.17	4.88
laboratory	Amphiroa gracilis (macroalga)	1 week	Increased temperature	1.398	9.111								22.9	0.04	8.2	325	3	2762	5	38.3	0.04	7.5	0.04	4.9	0.03										8	8.84	300.25	301.23	1957.20	333.96	2300.00	5.13	7.80
laboratory	Amphiroa gracilis (macroalga)	1 week	Increased temperature	2.284	14.501								22.9	0.04	8.2	325	3	2762	5	38.3	0.04	7.5	0.04	4.9	0.03										8	8.84	300.25	301.23	1957.20	333.96	2300.00	5.13	7.80
laboratory	Amphiroa gracilis (macroalga)	1 week	Increased temperature	2.303	18.062								22.9	0.04	8.2	325	3	2762	5	38.3	0.04	7.5	0.04	4.9	0.03										8	8.84	300.25	301.23	1957.20	333.96	2300.00	5.13	7.80
laboratory	Amphiroa gracilis (macroalga)	1 week	Increased temperature	1.995	13.891								22.9	0.04	8.2	325	3	2762	5	38.3	0.04	7.5	0.04	4.9	0.03										8	8.84	300.25	301.23	1957.20	333.96	2300.00	5.13	7.80
laboratory	Amphiroa gracilis (macroalga)	1 week	Reduced pH	2.898	17.176								19.1	0.04	7.8	746	18	2319	4	37.5	0.05	2.8	0.05	1.8	0.03										8	25.04	763.85	766.48	2027.13	117.44	2169.62	1.79	2.75
laboratory	Amphiroa gracilis (macroalga)	1 week	Reduced pH	1.367	10.584								19.1	0.04	7.8	746	18	2319	4	37.5	0.05	2.8	0.05	1.8	0.03										8	25.04	763.85	766.48	2027.13	117.44	2169.62	1.79	2.75
laboratory	Amphiroa gracilis (macroalga)	1 week	Reduced pH	3.954	24.913								19.1	0.04	7.8	746	18	2319	4	37.5	0.05	2.8	0.05	1.8	0.03										8	25.04	763.85	766.48	2027.13	117.44	2169.62	1.79	2.75
laboratory	Amphiroa gracilis (macroalga)	1 week	Reduced pH	2.740	17.558								19.1	0.04	7.8	746	18	2319	4	37.5	0.05	2.8	0.05	1.8	0.03										8	25.04	763.85	766.48	2027.13	117.44	2169.62	1.79	2.75
laboratory	Amphiroa gracilis (macroalga)	1 week	Increased temperature and reduced pH	1.941	18.789								22.9	0.04	7.8	889	29	2574	5	36.5	0.11	3.4	0.08	2.2	0.05										8	25.53	858.76	861.59	2222.69	145.20	2393.43	2.26	3.44
laboratory	Amphiroa gracilis (macroalga)	1 week	Increased temperature and reduced pH	1.823	15.432								22.9	0.04	7.8	889	29	2574	5	36.5	0.11	3.4	0.08	2.2	0.05										8	25.53	858.76	861.59	2222.69	145.20	2393.43	2.26	3.44
laboratory	Amphiroa gracilis (macroalga)	1 week	Increased temperature and reduced pH	2.428	17.156								22.9	0.04	7.8	889	29	2574	5	36.5	0.11	3.4	0.08	2.2	0.05										8	25.53	858.76	861.59	2222.69	145.20	2393.43	2.26	3.44
laboratory	Amphiroa gracilis (macroalga)	1 week	Increased temperature and reduced pH	1.801	13.323								22.9	0.04	7.8	889	29	2574	5	36.5	0.11	3.4	0.08	2.2	0.05										8	25.53	858.76	861.59	2222.69	145.20	2393.43	2.26	3.44
laboratory	Amphiroa gracilis (macroalga)	2 weeks	Ambient	1.463	13.929								19.3	0.04	8.1	325	3	2393	20	37.3	0.04	5.3	0.10	3.5	0.06										8	11.48	351.80	353.01	1859.97	215.69	2087.15	3.30	5.07
laboratory	Amphiroa gracilis (macroalga)	2 weeks	Ambient	2.059	18.733								19.3	0.04	8.1	325	3	2393	20	37.3	0.04	5.3	0.10	3.5	0.06										8	11.48	351.80	353.01	1859.97	215.69	2087.15	3.30	5.07
laboratory	Amphiroa gracilis (macroalga)	2 weeks	Ambient	2.865	17.269								19.3	0.04	8.1	325	3	2393	20	37.3	0.04	5.3	0.10	3.5	0.06										8	11.48	351.80	353.01	1859.97	215.69	2087.15	3.30	5.07
laboratory	Amphiroa gracilis (macroalga)	2 weeks	Ambient	2.194	16.889								19.3	0.04	8.1	325	3	2393	20	37.3	0.04	5.3	0.10	3.5	0.06										8	11.48	351.80	353.01	1859.97	215.69	2087.15	3.30	5.07
laboratory	Amphiroa gracilis (macroalga)	2 weeks	Increased temperature	1.754	13.534								23.5	0.03	8.2	280	2	2930	4	37.4	0.08	9.1	0.05	6.0	0.03										8	9.35	320.97	322.01	2082.53	356.37	2448.25	5.52	8.39
laboratory	Amphiroa gracilis (macroalga)	2 weeks	Increased temperature	2.051	13.852								23.5	0.03	8.2	280	2	2930	4	37.4	0.08	9.1	0.05	6.0	0.03										8	9.35	320.97	322.01	2082.53	356.37	2448.25	5.52	8.39
laboratory	Amphiroa gracilis (macroalga)	2 weeks	Increased temperature	2.109	17.418								23.5	0.03	8.2	280	2	2930	4	37.4	0.08	9.1	0.05	6.0	0.03										8	9.35	320.97	322.01	2082.53	356.37	2448.25	5.52	8.39
laboratory	Amphiroa gracilis (macroalga)	2 weeks	Increased temperature	1.760	16.149								23.5	0.03	8.2	280	2	2930	4	37.4	0.08	9.1	0.05	6.0	0.03										8	9.35	320.97	322.01	2082.53	356.37	2448.25	5.52	8.39
laboratory	Amphiroa gracilis (macroalga)	2 weeks	Reduced pH	2.677	17.869								19.5	0.04	7.8	816	10	2398	3	37.1	0.07	2.8	0.03	1.8	0.02										8	25.75	792.51	795.22	2096.72	122.25	2244.72	1.87	2.88
laboratory	Amphiroa gracilis (macroalga)	2 weeks	Reduced pH	3.596	32.396								19.5	0.04	7.8	816	10	2398	3	37.1	0.07	2.8	0.03	1.8	0.02										8	25.75	792.51	795.22	2096.72	122.25	2244.72	1.87	2.88
laboratory	Amphiroa gracilis (macroalga)	2 weeks	Reduced pH	3.397	25.812								19.5	0.04	7.8	816	10	2398	3	37.1	0.07	2.8	0.03	1.8	0.02										8	25.75	792.51	795.22	2096.72	122.25	2244.72	1.87	2.88
laboratory	Amphiroa gracilis (macroalga)	2 weeks	Reduced pH	2.432	27.601								19.5	0.04	7.8	816	10	2398	3	37.1	0.07	2.8	0.03	1.8	0.02										8	25.75	792.51	795.22	2096.72	122.25	2244.72	1.87	2.88
laboratory	Amphiroa gracilis (macroalga)	2 weeks	Increased temperature and reduced pH	2.190	17.045								23.4	0.04	7.8	1013	20	2850	2	37.5	0.20	3.8	0.06	2.5	0.04										8	27.70	948.71	951.81	2452.37	166.81	2646.87	2.58	3.93
laboratory	Amphiroa gracilis (macroalga)	2 weeks	Increased temperature and reduced pH	2.241	19.087								23.4	0.04	7.8	1013	20	2850	2	37.5	0.20	3.8	0.06	2.5	0.04										8	27.70	948.71	951.81	2452.37	166.81	2646.87	2.58	3.93
laboratory	Amphiroa gracilis (macroalga)	2 weeks	Increased temperature and reduced pH	1.908	15.032								23.4	0.04	7.8	1013	20	2850	2	37.5	0.20	3.8	0.06	2.5	0.04										8	27.70	948.71	951.81	2452.37	166.81	2646.87	2.58	3.93
laboratory	Amphiroa gracilis (macroalga)	2 weeks	Increased temperature and reduced pH	1.932	17.918								23.4	0.04	7.8	1013	20	2850	2	37.5	0.20	3.8	0.06	2.5	0.04										8	27.70	948.71	951.81	2452.37	166.81	2646.87	2.58	3.93
laboratory	Amphiroa gracilis (macroalga)	3 weeks	Ambient	1.541	10.574								19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Amphiroa gracilis (macroalga)	3 weeks	Ambient	1.809	24.440								19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Amphiroa gracilis (macroalga)	3 weeks	Ambient	1.648	12.421								19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Amphiroa gracilis (macroalga)	3 weeks	Ambient	3.064	23.605								19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Amphiroa gracilis (macroalga)	3 weeks	Ambient	2.872	31.732								19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Amphiroa gracilis (macroalga)	3 weeks	Increased temperature	2.749	26.336								22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Amphiroa gracilis (macroalga)	3 weeks	Increased temperature	4.382	40.131								22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Amphiroa gracilis (macroalga)	3 weeks	Increased temperature	2.127	15.433								22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Amphiroa gracilis (macroalga)	3 weeks	Increased temperature	2.071	30.585								22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Amphiroa gracilis (macroalga)	3 weeks	Reduced pH	2.614	21.251								19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Amphiroa gracilis (macroalga)	3 weeks	Reduced pH	3.094	23.532								19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Amphiroa gracilis (macroalga)	3 weeks	Reduced pH	3.773	26.019								19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Amphiroa gracilis (macroalga)	3 weeks	Reduced pH	1.671	12.093								19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Amphiroa gracilis (macroalga)	3 weeks	Reduced pH	2.868	26.931								19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Amphiroa gracilis (macroalga)	3 weeks	Increased temperature and reduced pH	2.458	23.935								22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Amphiroa gracilis (macroalga)	3 weeks	Increased temperature and reduced pH	2.975	22.503								22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Amphiroa gracilis (macroalga)	3 weeks	Increased temperature and reduced pH	2.417	20.763								22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Amphiroa gracilis (macroalga)	3 weeks	Increased temperature and reduced pH	6.196	29.801								22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Amphiroa gracilis (macroalga)	3 weeks	Increased temperature and reduced pH	2.746	25.959								22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient			1	100	0	0	0			19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient			2	100	0	0	0			19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient			3	100	0	0	0			19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient			9	100	0	0	0			19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient			10	100	0	0	0			19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient			11	100	0	0	0			19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient			12	100	0	0	0			19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient			13	99	1	0	1			19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient			14	99	1	0	1			19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient			15	100	0	0	0			19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature			21	27	44	29	73			22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature			22	44	31	25	56			22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature			23	39	33	28	61			22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature			24	76	13	11	24			22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature			25	82	10	8	18			22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature			26	36	46	18	64			22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature			27	38	35	27	62			22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature			28	33	30	37	67			22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature			29	29	33	38	71			22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature			30	65	27	8	35			22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH			41	61	36	3	39			19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH			42	41	53	6	59			19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH			43	76	16	8	24			19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH			44	60	31	9	40			19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH			45	44	56	0	56			19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH			46	72	28	0	28			19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH			47	47	53	0	53			19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH			48	60	40	0	40			19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH			49	64	36	0	36			19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH			50	63	37	0	37			19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH			61	61	39	0	39			22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH			62	65	35	0	35			22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH			63	67	33	0	33			22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH			64	79	21	0	21			22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH			65	61	39	0	39			22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH			66	70	15	15	30			22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH			68	50	27	23	50			22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH			69	45	34	21	55			22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH			70	66	22	12	34			22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH			73	41	38	21	59			22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Ambient								1	75.00	19.3	0.04	8.1	325	3	2393	20	37.3	0.04	5.3	0.10	3.5	0.06										8	11.48	351.80	353.01	1859.97	215.69	2087.15	3.30	5.07
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Ambient								2	60.00	19.3	0.04	8.1	325	3	2393	20	37.3	0.04	5.3	0.10	3.5	0.06										8	11.48	351.80	353.01	1859.97	215.69	2087.15	3.30	5.07
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Ambient								3	60.00	19.3	0.04	8.1	325	3	2393	20	37.3	0.04	5.3	0.10	3.5	0.06										8	11.48	351.80	353.01	1859.97	215.69	2087.15	3.30	5.07
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Ambient								4	60.00	19.3	0.04	8.1	325	3	2393	20	37.3	0.04	5.3	0.10	3.5	0.06										8	11.48	351.80	353.01	1859.97	215.69	2087.15	3.30	5.07
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Ambient								5	60.00	19.3	0.04	8.1	325	3	2393	20	37.3	0.04	5.3	0.10	3.5	0.06										8	11.48	351.80	353.01	1859.97	215.69	2087.15	3.30	5.07
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Ambient								6	50.00	19.3	0.04	8.1	325	3	2393	20	37.3	0.04	5.3	0.10	3.5	0.06										8	11.48	351.80	353.01	1859.97	215.69	2087.15	3.30	5.07
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Ambient								7	75.00	19.3	0.04	8.1	325	3	2393	20	37.3	0.04	5.3	0.10	3.5	0.06										8	11.48	351.80	353.01	1859.97	215.69	2087.15	3.30	5.07
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Ambient								8	80.00	19.3	0.04	8.1	325	3	2393	20	37.3	0.04	5.3	0.10	3.5	0.06										8	11.48	351.80	353.01	1859.97	215.69	2087.15	3.30	5.07
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Ambient								9	0.00	19.3	0.04	8.1	325	3	2393	20	37.3	0.04	5.3	0.10	3.5	0.06										8	11.48	351.80	353.01	1859.97	215.69	2087.15	3.30	5.07
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Ambient								10	60.00	19.3	0.04	8.1	325	3	2393	20	37.3	0.04	5.3	0.10	3.5	0.06										8	11.48	351.80	353.01	1859.97	215.69	2087.15	3.30	5.07
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Ambient								11	20.00	19.3	0.04	8.1	325	3	2393	20	37.3	0.04	5.3	0.10	3.5	0.06										8	11.48	351.80	353.01	1859.97	215.69	2087.15	3.30	5.07
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Ambient								12	60.00	19.3	0.04	8.1	325	3	2393	20	37.3	0.04	5.3	0.10	3.5	0.06										8	11.48	351.80	353.01	1859.97	215.69	2087.15	3.30	5.07
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Ambient								13	40.00	19.3	0.04	8.1	325	3	2393	20	37.3	0.04	5.3	0.10	3.5	0.06										8	11.48	351.80	353.01	1859.97	215.69	2087.15	3.30	5.07
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Ambient								14	60.00	19.3	0.04	8.1	325	3	2393	20	37.3	0.04	5.3	0.10	3.5	0.06										8	11.48	351.80	353.01	1859.97	215.69	2087.15	3.30	5.07
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Ambient								15	75.00	19.3	0.04	8.1	325	3	2393	20	37.3	0.04	5.3	0.10	3.5	0.06										8	11.48	351.80	353.01	1859.97	215.69	2087.15	3.30	5.07
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Ambient								16	20.00	19.3	0.04	8.1	325	3	2393	20	37.3	0.04	5.3	0.10	3.5	0.06										8	11.48	351.80	353.01	1859.97	215.69	2087.15	3.30	5.07
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Ambient								17	80.00	19.3	0.04	8.1	325	3	2393	20	37.3	0.04	5.3	0.10	3.5	0.06										8	11.48	351.80	353.01	1859.97	215.69	2087.15	3.30	5.07
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Ambient								18	40.00	19.3	0.04	8.1	325	3	2393	20	37.3	0.04	5.3	0.10	3.5	0.06										8	11.48	351.80	353.01	1859.97	215.69	2087.15	3.30	5.07
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Ambient								19	100.00	19.3	0.04	8.1	325	3	2393	20	37.3	0.04	5.3	0.10	3.5	0.06										8	11.48	351.80	353.01	1859.97	215.69	2087.15	3.30	5.07
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Ambient								20	40.00	19.3	0.04	8.1	325	3	2393	20	37.3	0.04	5.3	0.10	3.5	0.06										8	11.48	351.80	353.01	1859.97	215.69	2087.15	3.30	5.07
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature								21	20.00	23.5	0.03	8.2	280	2	2930	4	37.4	0.08	9.1	0.05	6.0	0.03										8	9.35	320.97	322.01	2082.53	356.37	2448.25	5.52	8.39
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature								22	0.00	23.5	0.03	8.2	280	2	2930	4	37.4	0.08	9.1	0.05	6.0	0.03										8	9.35	320.97	322.01	2082.53	356.37	2448.25	5.52	8.39
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature								23	40.00	23.5	0.03	8.2	280	2	2930	4	37.4	0.08	9.1	0.05	6.0	0.03										8	9.35	320.97	322.01	2082.53	356.37	2448.25	5.52	8.39
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature								24	80.00	23.5	0.03	8.2	280	2	2930	4	37.4	0.08	9.1	0.05	6.0	0.03										8	9.35	320.97	322.01	2082.53	356.37	2448.25	5.52	8.39
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature								25	14.29	23.5	0.03	8.2	280	2	2930	4	37.4	0.08	9.1	0.05	6.0	0.03										8	9.35	320.97	322.01	2082.53	356.37	2448.25	5.52	8.39
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature								26	20.00	23.5	0.03	8.2	280	2	2930	4	37.4	0.08	9.1	0.05	6.0	0.03										8	9.35	320.97	322.01	2082.53	356.37	2448.25	5.52	8.39
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature								27	0.00	23.5	0.03	8.2	280	2	2930	4	37.4	0.08	9.1	0.05	6.0	0.03										8	9.35	320.97	322.01	2082.53	356.37	2448.25	5.52	8.39
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature								28	0.00	23.5	0.03	8.2	280	2	2930	4	37.4	0.08	9.1	0.05	6.0	0.03										8	9.35	320.97	322.01	2082.53	356.37	2448.25	5.52	8.39
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature								29	40.00	23.5	0.03	8.2	280	2	2930	4	37.4	0.08	9.1	0.05	6.0	0.03										8	9.35	320.97	322.01	2082.53	356.37	2448.25	5.52	8.39
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature								30	60.00	23.5	0.03	8.2	280	2	2930	4	37.4	0.08	9.1	0.05	6.0	0.03										8	9.35	320.97	322.01	2082.53	356.37	2448.25	5.52	8.39
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature								31	40.00	23.5	0.03	8.2	280	2	2930	4	37.4	0.08	9.1	0.05	6.0	0.03										8	9.35	320.97	322.01	2082.53	356.37	2448.25	5.52	8.39
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature								32	0.00	23.5	0.03	8.2	280	2	2930	4	37.4	0.08	9.1	0.05	6.0	0.03										8	9.35	320.97	322.01	2082.53	356.37	2448.25	5.52	8.39
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature								33	0.00	23.5	0.03	8.2	280	2	2930	4	37.4	0.08	9.1	0.05	6.0	0.03										8	9.35	320.97	322.01	2082.53	356.37	2448.25	5.52	8.39
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature								34	60.00	23.5	0.03	8.2	280	2	2930	4	37.4	0.08	9.1	0.05	6.0	0.03										8	9.35	320.97	322.01	2082.53	356.37	2448.25	5.52	8.39
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature								35	20.00	23.5	0.03	8.2	280	2	2930	4	37.4	0.08	9.1	0.05	6.0	0.03										8	9.35	320.97	322.01	2082.53	356.37	2448.25	5.52	8.39
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature								36	40.00	23.5	0.03	8.2	280	2	2930	4	37.4	0.08	9.1	0.05	6.0	0.03										8	9.35	320.97	322.01	2082.53	356.37	2448.25	5.52	8.39
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature								37	0.00	23.5	0.03	8.2	280	2	2930	4	37.4	0.08	9.1	0.05	6.0	0.03										8	9.35	320.97	322.01	2082.53	356.37	2448.25	5.52	8.39
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature								38	0.00	23.5	0.03	8.2	280	2	2930	4	37.4	0.08	9.1	0.05	6.0	0.03										8	9.35	320.97	322.01	2082.53	356.37	2448.25	5.52	8.39
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature								39	20.00	23.5	0.03	8.2	280	2	2930	4	37.4	0.08	9.1	0.05	6.0	0.03										8	9.35	320.97	322.01	2082.53	356.37	2448.25	5.52	8.39
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature								40	40.00	23.5	0.03	8.2	280	2	2930	4	37.4	0.08	9.1	0.05	6.0	0.03										8	9.35	320.97	322.01	2082.53	356.37	2448.25	5.52	8.39
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Reduced pH								41	0.00	19.5	0.04	7.8	816	10	2398	3	37.1	0.07	2.8	0.03	1.8	0.02										8	25.75	792.51	795.22	2096.72	122.25	2244.72	1.87	2.88
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Reduced pH								42	60.00	19.5	0.04	7.8	816	10	2398	3	37.1	0.07	2.8	0.03	1.8	0.02										8	25.75	792.51	795.22	2096.72	122.25	2244.72	1.87	2.88
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Reduced pH								43	20.00	19.5	0.04	7.8	816	10	2398	3	37.1	0.07	2.8	0.03	1.8	0.02										8	25.75	792.51	795.22	2096.72	122.25	2244.72	1.87	2.88
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Reduced pH								44	20.00	19.5	0.04	7.8	816	10	2398	3	37.1	0.07	2.8	0.03	1.8	0.02										8	25.75	792.51	795.22	2096.72	122.25	2244.72	1.87	2.88
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Reduced pH								45	40.00	19.5	0.04	7.8	816	10	2398	3	37.1	0.07	2.8	0.03	1.8	0.02										8	25.75	792.51	795.22	2096.72	122.25	2244.72	1.87	2.88
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Reduced pH								46	40.00	19.5	0.04	7.8	816	10	2398	3	37.1	0.07	2.8	0.03	1.8	0.02										8	25.75	792.51	795.22	2096.72	122.25	2244.72	1.87	2.88
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Reduced pH								47	20.00	19.5	0.04	7.8	816	10	2398	3	37.1	0.07	2.8	0.03	1.8	0.02										8	25.75	792.51	795.22	2096.72	122.25	2244.72	1.87	2.88
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Reduced pH								48	40.00	19.5	0.04	7.8	816	10	2398	3	37.1	0.07	2.8	0.03	1.8	0.02										8	25.75	792.51	795.22	2096.72	122.25	2244.72	1.87	2.88
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Reduced pH								49	20.00	19.5	0.04	7.8	816	10	2398	3	37.1	0.07	2.8	0.03	1.8	0.02										8	25.75	792.51	795.22	2096.72	122.25	2244.72	1.87	2.88
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Reduced pH								50	40.00	19.5	0.04	7.8	816	10	2398	3	37.1	0.07	2.8	0.03	1.8	0.02										8	25.75	792.51	795.22	2096.72	122.25	2244.72	1.87	2.88
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Reduced pH								51	100.00	19.5	0.04	7.8	816	10	2398	3	37.1	0.07	2.8	0.03	1.8	0.02										8	25.75	792.51	795.22	2096.72	122.25	2244.72	1.87	2.88
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Reduced pH								52	40.00	19.5	0.04	7.8	816	10	2398	3	37.1	0.07	2.8	0.03	1.8	0.02										8	25.75	792.51	795.22	2096.72	122.25	2244.72	1.87	2.88
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Reduced pH								53	60.00	19.5	0.04	7.8	816	10	2398	3	37.1	0.07	2.8	0.03	1.8	0.02										8	25.75	792.51	795.22	2096.72	122.25	2244.72	1.87	2.88
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Reduced pH								54	40.00	19.5	0.04	7.8	816	10	2398	3	37.1	0.07	2.8	0.03	1.8	0.02										8	25.75	792.51	795.22	2096.72	122.25	2244.72	1.87	2.88
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Reduced pH								55	60.00	19.5	0.04	7.8	816	10	2398	3	37.1	0.07	2.8	0.03	1.8	0.02										8	25.75	792.51	795.22	2096.72	122.25	2244.72	1.87	2.88
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Reduced pH								56	20.00	19.5	0.04	7.8	816	10	2398	3	37.1	0.07	2.8	0.03	1.8	0.02										8	25.75	792.51	795.22	2096.72	122.25	2244.72	1.87	2.88
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Reduced pH								57	20.00	19.5	0.04	7.8	816	10	2398	3	37.1	0.07	2.8	0.03	1.8	0.02										8	25.75	792.51	795.22	2096.72	122.25	2244.72	1.87	2.88
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Reduced pH								58	20.00	19.5	0.04	7.8	816	10	2398	3	37.1	0.07	2.8	0.03	1.8	0.02										8	25.75	792.51	795.22	2096.72	122.25	2244.72	1.87	2.88
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Reduced pH								59	60.00	19.5	0.04	7.8	816	10	2398	3	37.1	0.07	2.8	0.03	1.8	0.02										8	25.75	792.51	795.22	2096.72	122.25	2244.72	1.87	2.88
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Reduced pH								60	80.00	19.5	0.04	7.8	816	10	2398	3	37.1	0.07	2.8	0.03	1.8	0.02										8	25.75	792.51	795.22	2096.72	122.25	2244.72	1.87	2.88
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature and reduced pH								61	40.00	23.4	0.04	7.8	1013	20	2850	2	37.5	0.20	3.8	0.06	2.5	0.04										8	27.70	948.71	951.81	2452.37	166.81	2646.87	2.58	3.93
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature and reduced pH								62	40.00	23.4	0.04	7.8	1013	20	2850	2	37.5	0.20	3.8	0.06	2.5	0.04										8	27.70	948.71	951.81	2452.37	166.81	2646.87	2.58	3.93
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature and reduced pH								63	66.67	23.4	0.04	7.8	1013	20	2850	2	37.5	0.20	3.8	0.06	2.5	0.04										8	27.70	948.71	951.81	2452.37	166.81	2646.87	2.58	3.93
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature and reduced pH								64	20.00	23.4	0.04	7.8	1013	20	2850	2	37.5	0.20	3.8	0.06	2.5	0.04										8	27.70	948.71	951.81	2452.37	166.81	2646.87	2.58	3.93
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature and reduced pH								65	0.00	23.4	0.04	7.8	1013	20	2850	2	37.5	0.20	3.8	0.06	2.5	0.04										8	27.70	948.71	951.81	2452.37	166.81	2646.87	2.58	3.93
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature and reduced pH								66	20.00	23.4	0.04	7.8	1013	20	2850	2	37.5	0.20	3.8	0.06	2.5	0.04										8	27.70	948.71	951.81	2452.37	166.81	2646.87	2.58	3.93
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature and reduced pH								67	40.00	23.4	0.04	7.8	1013	20	2850	2	37.5	0.20	3.8	0.06	2.5	0.04										8	27.70	948.71	951.81	2452.37	166.81	2646.87	2.58	3.93
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature and reduced pH								68	0.00	23.4	0.04	7.8	1013	20	2850	2	37.5	0.20	3.8	0.06	2.5	0.04										8	27.70	948.71	951.81	2452.37	166.81	2646.87	2.58	3.93
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature and reduced pH								69	50.00	23.4	0.04	7.8	1013	20	2850	2	37.5	0.20	3.8	0.06	2.5	0.04										8	27.70	948.71	951.81	2452.37	166.81	2646.87	2.58	3.93
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature and reduced pH								70	0.00	23.4	0.04	7.8	1013	20	2850	2	37.5	0.20	3.8	0.06	2.5	0.04										8	27.70	948.71	951.81	2452.37	166.81	2646.87	2.58	3.93
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature and reduced pH								71		23.4	0.04	7.8	1013	20	2850	2	37.5	0.20	3.8	0.06	2.5	0.04										8	27.70	948.71	951.81	2452.37	166.81	2646.87	2.58	3.93
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature and reduced pH								72		23.4	0.04	7.8	1013	20	2850	2	37.5	0.20	3.8	0.06	2.5	0.04										8	27.70	948.71	951.81	2452.37	166.81	2646.87	2.58	3.93
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature and reduced pH								73	40.00	23.4	0.04	7.8	1013	20	2850	2	37.5	0.20	3.8	0.06	2.5	0.04										8	27.70	948.71	951.81	2452.37	166.81	2646.87	2.58	3.93
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature and reduced pH								74	60.00	23.4	0.04	7.8	1013	20	2850	2	37.5	0.20	3.8	0.06	2.5	0.04										8	27.70	948.71	951.81	2452.37	166.81	2646.87	2.58	3.93
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature and reduced pH								75	28.57	23.4	0.04	7.8	1013	20	2850	2	37.5	0.20	3.8	0.06	2.5	0.04										8	27.70	948.71	951.81	2452.37	166.81	2646.87	2.58	3.93
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature and reduced pH								76	40.00	23.4	0.04	7.8	1013	20	2850	2	37.5	0.20	3.8	0.06	2.5	0.04										8	27.70	948.71	951.81	2452.37	166.81	2646.87	2.58	3.93
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature and reduced pH								77	40.00	23.4	0.04	7.8	1013	20	2850	2	37.5	0.20	3.8	0.06	2.5	0.04										8	27.70	948.71	951.81	2452.37	166.81	2646.87	2.58	3.93
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature and reduced pH								78	60.00	23.4	0.04	7.8	1013	20	2850	2	37.5	0.20	3.8	0.06	2.5	0.04										8	27.70	948.71	951.81	2452.37	166.81	2646.87	2.58	3.93
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature and reduced pH								79	20.00	23.4	0.04	7.8	1013	20	2850	2	37.5	0.20	3.8	0.06	2.5	0.04										8	27.70	948.71	951.81	2452.37	166.81	2646.87	2.58	3.93
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks	Increased temperature and reduced pH								80	20.00	23.4	0.04	7.8	1013	20	2850	2	37.5	0.20	3.8	0.06	2.5	0.04										8	27.70	948.71	951.81	2452.37	166.81	2646.87	2.58	3.93
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks									FSW	40.00
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks									FSW	20.00
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks									FSW	0.00
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks									FSW	0.00
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks									FSW	0.00
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks									FSW	0.00
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks									FSW	20.00
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks									FSW	0.00
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks									FSW	33.33
laboratory	Heliocidaris erythogramma (echinoderm)	2 weeks									FSW	0.00
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient								1	20.00	19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient								2	0.00	19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient								3	100.00	19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient								4	60.00	19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient								5	66.66	19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient								6	20.00	19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient								7	0.00	19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient								8	20.00	19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient								9	50.00	19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient								10	20.00	19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient								11	0.00	19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient								12	40.00	19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient								13	50.00	19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient								14	60.00	19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient								15	40.00	19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient								16	80.00	19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient								17	80.00	19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient								18	33.33	19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient								19	40.00	19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient								20	40.00	19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01										8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature								21	40.00	22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature								22	0.00	22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature								23	40.00	22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature								24	20.00	22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature								25	0.00	22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature								26	0.00	22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature								27	0.00	22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature								28	0.00	22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature								29	20.00	22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature								30	40.00	22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature								31	40.00	22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature								32	20.00	22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature								33	0.00	22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature								34	40.00	22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature								35	0.00	22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature								36	0.00	22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature								37	0.00	22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature								38	0.00	22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature								39	40.00	22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature								40	40.00	22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02										8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH								41	40.00	19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH								42	20.00	19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH								43	60.00	19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH								44	60.00	19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH								45	20.00	19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH								46	40.00	19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH								47	40.00	19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH								48	0.00	19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH								49	40.00	19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH								50	20.00	19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH								51	40.00	19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH								52	60.00	19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH								53	60.00	19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH								54	20.00	19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH								55	20.00	19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH								56	20.00	19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH								57	40.00	19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH								58	40.00	19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH								59	0.00	19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH								60	20.00	19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06										8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH								61	0.00	22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH								62	20.00	22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH								63	20.00	22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH								64	0.00	22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH								65	60.00	22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH								66	20.00	22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH								67	20.00	22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH								68	20.00	22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH								69	33.33	22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH								70	20.00	22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH								71		22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH								72		22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH								73	0.00	22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH								74	20.00	22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH								75	0.00	22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH								76	0.00	22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH								77	60.00	22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH								78	0.00	22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH								79	40.00	22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH								80	20.00	22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01										8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks									FSW	0.00
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks									FSW	0.00
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks									FSW	0.00
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks									FSW	0.00
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks									FSW	0.00
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks									FSW	0.00
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks									FSW	0.00
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks									FSW	0.00
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks									FSW	0.00
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks									FSW	0.00
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient										19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01	4	436.7	24.3	479.15	54.92	0.65	0.01	3.96	0.06	8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Ambient										19.3	0.03	8.1	342	3	2420	2	36.8	0.11	5.3	0.02	3.4	0.01	12							3.64	0.07	8	11.69	357.22	358.45	1887.19	216.47	2115.35	3.32	5.11
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature										22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02	4	400.0	27.0	444.18	61.03	0.65	0.01	3.91	0.06	8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature										22.6	0.03	8.2	289	3	2967	6	37.4	0.06	8.9	0.03	5.9	0.02	12							3.76	0.11	8	9.74	326.54	327.61	2130.04	352.55	2492.33	5.45	8.30
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH										19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06	5	456.0	42.6	519.80	112.84	0.63	0.03	3.82	0.22	8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Reduced pH										19.3	0.03	7.9	650	24	2438	3	36.9	0.05	3.4	0.09	2.2	0.06	12							3.46	0.07	8	20.30	620.69	622.82	2069.31	150.09	2239.70	2.30	3.54
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH										22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01	4	439.0	27.7	496.21	55.07	0.66	0.02	4.00	0.15	8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89
laboratory	Heliocidaris erythogramma (echinoderm)	3 weeks	Increased temperature and reduced pH										22.6	0.03	7.8	1059	10	2904	8	37.4	0.10	3.6	0.02	2.4	0.01	12							3.91	0.09	8	28.84	966.81	970.00	2510.71	165.44	2704.98	2.56	3.89