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Giguet-Covex, Charline (2023): Intensity index of agro-pastoral activities reconstructed from Lake La Thuile sediments, over the last 6400 years [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.958960, In: Giguet-Covex, Charline; Chen, Wentao; Ficetola, Francesco Gentile; Gielly, Ludovic; Pansu, Johan (2023): Plant DNA analyses, nature and intensity of human activities, and erosion intensities in the North-western Alps over the last 4000-6000 years inferred from lake sediments [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.958732

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Keyword(s):
Agriculture; DNA metabarcoding; human activity intensity; lake sediment DNA; lake sediments; pastoralism
Related to:
Giguet-Covex, Charline; Bajard, Manon; Chen, Wentao; Walsh, Kevin; Rey, Pierre-Jérôme; Messager, Erwan; Etienne, David; Sabatier, Pierre; Ficetola, Francesco Gentile; Gielly, Ludovic; Blanchet, C; Guffond, Christophe; Chiquet, Patricia; Arnaud, Fabien; Poulenard, Jérôme (2023): Long-term trajectories of mountain agro-ecosystems in the North-Western Alps. Regional Environmental Change, 23(2), 58, https://doi.org/10.1007/s10113-023-02030-5
Coverage:
Latitude: 45.530587 * Longitude: 6.056265
Date/Time Start: 2010-04-26T00:00:00 * Date/Time End: 2010-04-26T00:00:00
Minimum Elevation: 874.0 m * Maximum Elevation: 874.0 m
Event(s):
THU10_I * Latitude: 45.530587 * Longitude: 6.056265 * Date/Time: 2010-04-26T00:00:00 * Elevation: 874.0 m * Location: North-western Alps * Method/Device: Sediment corer (SEDCO)
Comment:
Method used to calculate the index: This index has been calculated on the basis of agro-pastoral indicators obtained from the DNA metabarcoding approach targeting plants (ruderal/nitrophilous taxa associated with pastoralism and crops) and mammals (for domesticated animals). The selected agro-pastoral indicators, here, are Plantago sp., Rumex sp., Cannabis sativa, Vicia faba, Juglandaceae, Pyrus sp., Prunus sp., and Vitaceae for plants, and Bos and Ovis for mammals. They are standardized by calculating a z-score. Then, the index is calculated as the average of all these standardized indicators.
Method used to generate sediment DNA datasets: DNA sequences were generated using g (5'-GGGCAATCCTGAGCCAA-3') and h (5'-CCATTGAGTCTCTGCACCTATC-3') primers for plants and MamP007F (5'-CGAGAAGACCCTATGGAGCT-3') and MamP007R (5'-CCGAGGTCRCCCCAACC-3') primers and the human-blocking primer (5'-GGAGCTTTAATTTATTAATGCAAACAGTACCC-3') for mammals. The sequences have been produced by the Illumina technology (HiSeq instrument). The bioinformatic treatment of the sequences was operated applying the Obitool program (Boyer, F. et al. OBITOOLS:aUNIX-inspired software package for DNAmetabarcoding. Mol. Ecol. Resour. 16, 176–182 (2016)) and following the procedure described in Giguet-Covex et al. New insights on lake sediment DNA from the catchment: importance of taphonomic and analytical issues on the record quality. Scientific Reports. 9, 14676 (2019) (DOI https://doi.org/10.1038/s41598-019-50339-1). Further filtering treatments were then applied, as also described in Giguet-Covex et al. 2019. These second treatments allow the removal of 1) taxa assigned to a reference taxon at <95 % of sequence identity, 2) potential contaminations (based on sampling, extraction and PCR controls, and recognition of exotic taxa) and 3) stochastic detections. Multiple analytical replicates by samples were performed (4 for the plants and 12 for the mammals). Data are presented in number of positive replicates by sample. A replicate is considered as positive if more than 5 sequences are detected.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
LakeLakeGiguet-Covex, Charline
AGEAgeka BPGiguet-Covex, CharlineAge model, radiocarbon dates and short radionuclidesGeocode
AgeAgea AD/CEGiguet-Covex, CharlineAge model, radiocarbon dates and short radionuclides
Period, culturalPeriod culturalGiguet-Covex, Charline
Intensity, z-scoreIntensity zGiguet-Covex, CharlineCalculating the mean of z-scores of all taxa associated with pastoral activities
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
171 data points

Data

Download dataset as tab-delimited text — use the following character encoding:


Lake

Age [ka BP]

Age [a AD/CE]

Period cultural

Intensity z
La Thuile-0.0011951Contemporary-0.210940889
La Thuile0.1291821Late modern0.197037317
La Thuile0.2601690Early modern0.295780446
La Thuile0.3891561Early modern0.645194721
La Thuile0.5201430Late middle Age1.603078694
La Thuile0.6491301Late middle Age0.902121749
La Thuile0.7801170High middle Age1.613334601
La Thuile0.9101040High middle Age0.575085649
La Thuile1.039911High middle Age1.393870451
La Thuile1.170780Early middle Age0.035223006
La Thuile1.284666Early middle Age0.214521119
La Thuile1.430520Early middle Age-0.043652072
La Thuile1.557393End Roman period-Early Middle Age-0.340118368
La Thuile1.694256End Roman period-Early Middle Age0.047819562
La Thuile1.821129Roman period-0.221372232
La Thuile1.9473Roman period-0.450777399
La Thuile2.085-135Iron Age-0.400414512
La Thuile2.212-262Iron Age-0.168485959
La Thuile2.338-388Iron Age-0.420571371
La Thuile2.465-515Iron Age-0.194494584
La ThuileMid-Late Bronze Age-0.308719652
La Thuile3.359-1409Mid-Late Bronze Age-0.284894309
La Thuile3.862-1912Early Bronze Age-0.565598358
La Thuile3.946-1996Early Bronze Age-0.573990070
La Thuile4.030-2080Early Bronze Age-0.582381783
La Thuile4.160-2210Neolithic-0.595368957
La Thuile4.290-2340Neolithic-0.597666688
La Thuile4.420-2470Neolithic-0.507100005
La Thuile4.550-2600Neolithic-0.436979621
La Thuile4.680-2730Neolithic-0.597666688
La Thuile4.810-2860Neolithic-0.507100005
La Thuile4.940-2990Neolithic-0.507100005
La Thuile5.075-3125Neolithic-0.597666688
La Thuile5.200-3250Neolithic-0.597666688
La Thuile5.330-3380Neolithic-0.507100005
La Thuile5.460-3510Pre anthropic-0.526664599
La Thuile5.590-3640Pre anthropic-0.526664599
La Thuile5.720-3770Pre anthropic-0.508083975
La Thuile5.850-3900Pre anthropic-0.485296418
La Thuile5.980-4030Pre anthropic-0.471711239
La Thuile6.110-4160Pre anthropic-0.469549701
La Thuile6.240-4290Pre anthropic-0.468437886
La Thuile6.370-4420Pre anthropic-0.479922740