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Giguet-Covex, Charline (2023): Intensity index of pastoral activities reconstructed from Lake Verney sediments, over the last 4600 years [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.958886, In: Giguet-Covex, Charline; Chen, Wentao; Ficetola, Francesco Gentile; Gielly, Ludovic; Pansu, Johan (2023): Plant DNA analyses, nature and intensity of human activities, and erosion intensities in the North-western Alps over the last 4000-6000 years inferred from lake sediments [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.958732

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Keyword(s):
Agriculture; DNA metabarcoding; human activity intensity; lake sediment DNA; lake sediments; pastoralism
Related to:
Giguet-Covex, Charline; Bajard, Manon; Chen, Wentao; Walsh, Kevin; Rey, Pierre-Jérôme; Messager, Erwan; Etienne, David; Sabatier, Pierre; Ficetola, Francesco Gentile; Gielly, Ludovic; Blanchet, C; Guffond, Christophe; Chiquet, Patricia; Arnaud, Fabien; Poulenard, Jérôme (2023): Long-term trajectories of mountain agro-ecosystems in the North-Western Alps. Regional Environmental Change, 23(2), 58, https://doi.org/10.1007/s10113-023-02030-5
Coverage:
Latitude: 45.687916 * Longitude: 6.057317
Minimum Elevation: 2090.0 m * Maximum Elevation: 2090.0 m
Event(s):
VER13_03 * Latitude: 45.687916 * Longitude: 6.057317 * Elevation: 2090.0 m * Method/Device: Sediment corer (SEDCO)
Comment:
Method used to calculate the index: This index has been calculated on the basis of pastoral indicators obtained from the DNA metabarcoding approach targeting plants (ruderal/nitrophilous taxa associated with pastoralism) and mammals (for domesticated animals). The selected pastoral indicators, here, are Plantago sp. and Rumex sp. for plants, and Bos and Ovis for mammals. They are standardized by calculating a z-score. Then, the index is calculated as the average of all these standardized indicators.
Method used to generate sediment DNA datasets: DNA sequences were generated using g (5'-GGGCAATCCTGAGCCAA-3') and h (5'-CCATTGAGTCTCTGCACCTATC-3') primers for plants and MamP007F (5'-CGAGAAGACCCTATGGAGCT-3') and MamP007R (5'-CCGAGGTCRCCCCAACC-3') primers and the human-blocking primer (5'-GGAGCTTTAATTTATTAATGCAAACAGTACCC-3') for mammals. The sequences have been produced by the Illumina technology (HiSeq instrument). The bioinformatic treatment of the sequences was operated applying the Obitool program (Boyer, F. et al. OBITOOLS:aUNIX-inspired software package for DNA metabarcoding. Mol. Ecol. Resour. 16, 176–182 (2016)) and following the procedure described in Giguet-Covex et al. New insights on lake sediment DNA from the catchment: importance of taphonomic and analytical issues on the record quality. Scientific Reports. 9, 14676 (2019) (DOI https://doi.org/10.1038/s41598-019-50339-1). Further filtering treatments were then applied, as also described in Giguet-Covex et al. 2019. These second treatments allow the removal of 1) taxa assigned to a reference taxon at <95 % of sequence identity, 2) potential contaminations (based on sampling, extraction and PCR controls, and recognition of exotic taxa) and 3) stochastic detections. Multiple analytical replicates by samples were performed (12 for both, the plants and mammals). Data are presented in number of positive replicates by sample. A replicate is considered as positive if more than 5 sequences are detected.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
LakeLakeGiguet-Covex, Charline
AGEAgeka BPGiguet-Covex, CharlineAge model, radiocarbon dates and short radionuclidesGeocode
AgeAgea AD/CEGiguet-Covex, CharlineAge model, radiocarbon dates and short radionuclides
Period, culturalPeriod culturalGiguet-Covex, Charline
Intensity, z-scoreIntensity zGiguet-Covex, CharlineCalculating the mean of z-scores of all taxa associated with pastoral activities
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
168 data points

Data

Download dataset as tab-delimited text — use the following character encoding:


Lake

Age [ka BP]

Age [a AD/CE]

Period cultural

Intensity z
Verney-0.05302003Contemporary-0.343409054
Verney-0.03951990Contemporary-0.414964658
Verney-0.02501975Contemporary-0.143734109
Verney-0.00601956Contemporary-0.129557785
Verney0.01851932Late modern-0.077143687
Verney0.04601904Late modern0.017784784
Verney0.07401876Late modern-0.317538657
Verney0.10501845Late modern-0.318753944
Verney0.15001800Late modern-0.147381137
Verney0.20501745Early modern-0.027383888
Verney0.27651674Early modern0.271104962
Verney0.34451606Early modern0.582643277
Verney0.39651554Early modern-0.169052901
Verney0.45351497Early modern0.817461203
Verney0.51101439Late middle age0.466418482
Verney0.56551385Late middle age0.111416230
Verney0.61901331Late middle age-0.065460872
Verney0.67251278Late middle age0.286701670
Verney0.72251228High middle age0.706389422
Verney0.77001180High middle age1.786184512
Verney0.81401136High middle age-0.202831462
Verney0.85301097High middle age0.541285953
Verney0.90251048High middle age-0.302621133
Verney0.9600990Early middle age-0.557834629
Verney1.0575893Early middle age0.053151342
Verney1.1610789Early middle age-0.394293865
Verney1.2835667Early middle age-0.072922084
Verney1.4395511Early middle age0.721554501
Verney1.5890361End Roman period-Early Middle Age-0.066819099
Verney1.7575193Roman period0.523752151
Verney1.9615-12Roman period-0.032243592
Verney2.2100-260Iron Age0.397073273
Verney2.6115-662Iron Age1.136318897
Verney3.0375-1088Mid-Late Bronze Age0.267113811
Verney3.3515-1402Mid-Late Bronze Age-0.368397664
Verney3.5675-1618Mid-Late Bronze Age-0.419202329
Verney3.7235-1774Early Bronze age-0.329736787
Verney3.9080-1958Early Bronze age-0.838606073
Verney4.0940-2144Early Bronze age-0.354684254
Verney4.2805-2331Neolithic-0.741271179
Verney4.4515-2502Neolithic-0.876072853
Verney4.6345-2685Neolithic-0.974436774