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Giguet-Covex, Charline (2023): Intensity index of pastoral activities reconstructed from Lake Gers sediments, over the last 4600 years [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.958885, In: Giguet-Covex, Charline; Chen, Wentao; Ficetola, Francesco Gentile; Gielly, Ludovic; Pansu, Johan (2023): Plant DNA analyses, nature and intensity of human activities, and erosion intensities in the North-western Alps over the last 4000-6000 years inferred from lake sediments [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.958732

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Keyword(s):
Agriculture; DNA metabarcoding; human activity intensity; lake sediment DNA; lake sediments; pastoralism; Sporormiella spores
Related to:
Giguet-Covex, Charline; Bajard, Manon; Chen, Wentao; Walsh, Kevin; Rey, Pierre-Jérôme; Messager, Erwan; Etienne, David; Sabatier, Pierre; Ficetola, Francesco Gentile; Gielly, Ludovic; Blanchet, C; Guffond, Christophe; Chiquet, Patricia; Arnaud, Fabien; Poulenard, Jérôme (2023): Long-term trajectories of mountain agro-ecosystems in the North-Western Alps. Regional Environmental Change, 23(2), 58, https://doi.org/10.1007/s10113-023-02030-5
Coverage:
Latitude: 46.027233 * Longitude: 6.729512
Date/Time Start: 2016-01-18T00:00:00 * Date/Time End: 2016-01-18T00:00:00
Minimum Elevation: 1532.0 m * Maximum Elevation: 1532.0 m
Event(s):
GER16 * Latitude: 46.027233 * Longitude: 6.729512 * Date/Time: 2016-01-18T00:00:00 * Elevation: 1532.0 m * Location: North-western Alps * Method/Device: Sediment corer (SEDCO)
Comment:
Method used to calculate the index: This index has been calculated on the basis of pastoral indicators obtained from the coprophilous fungi spore analysis and the DNA metabarcoding approach targeting plants (ruderal/nitrophilous taxa associated with pastoralism) and mammals (for domesticated animals). The selected pastoral indicators, here, are Sporormiella sp. for fungi spores, Plantago sp. and Rumex sp. for plants, and Bos, Ovis and Capra hircus for mammals. The index is calculated, after a transformation of each of these indicators (calculation of a z-score), as the average value of all these standardized indicators.
Method used to produce the sedimentary DNA datasets: DNA sequences were produced by using g (5'-GGGCAATCCTGAGCCAA-3') and h (5'-CCATTGAGTCTCTGCACCTATC-3') primers for plants and MamP007F (5'-CGAGAAGACCCTATGGAGCT-3') and MamP007R (5'-CCGAGGTCRCCCCAACC-3') primers and the human-blocking primer (5'-GGAGCTTTAATTTATTAATGCAAACAGTACCC-3') for mammals. The sequences have been produced by the Illumina technology (HiSeq instrument). The bioinformatic treatment of the sequences was operated applying the Obitool program (Boyer, F. et al. OBITOOLS:aUNIX-inspired software package for DNA metabarcoding. Mol. Ecol. Resour. 16, 176–182 (2016)) and following the procedure described in Giguet-Covex et al. New insights on lake sediment DNA from the catchment: importance of taphonomic and analytical issues on the record quality. Scientific Reports. 9, 14676 (2019) (DOI https://doi.org/10.1038/s41598-019-50339-1). Further filtering treatments were then applied, as also described in Giguet-Covex et al. 2019. These second treatments allow the removal of 1) taxa assigned to a reference taxon at <95 % of sequence identity, 2) potential contaminations (based on sampling, extraction and PCR controls, and recognition of exotic taxa) and 3) stochastic detections. Several analytical replicates by samples were performed (8 for both, the plants and mammals). Data are presented in number of positive replicates by sample. A replicate is considered as positive if more than 5 sequences are detected.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
LakeLakeGiguet-Covex, Charline
AGEAgeka BPGiguet-Covex, CharlineAge model, radiocarbon dates and short radionuclidesGeocode
AgeAgea AD/CEGiguet-Covex, CharlineAge model, radiocarbon dates and short radionuclides
Period, culturalPeriod culturalGiguet-Covex, Charline
Intensity, z-scoreIntensity zGiguet-Covex, CharlineCalculating the mean of z-scores of all taxa associated with pastoral activities
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
140 data points

Data

Download dataset as tab-delimited text — use the following character encoding:


Lake

Age [ka BP]

Age [a AD/CE]

Period cultural

Intensity z
Gers-0.05002000.0Contemporary-0.530507737
Gers0.07151878.5Late modern0.116348754
Gers0.12801822.0Late modern0.634057772
Gers0.22301727.0Early modern1.094578780
Gers0.35851591.5Early modern1.518675677
Gers0.45401496.0Early modern0.328427753
Gers0.55601394.0Late middle age0.324983835
Gers0.66801282.0Late middle age1.269199001
Gers0.71101239.0High middle age0.651381355
Gers0.75451195.5High middle age0.652332521
Gers0.81151138.5High middle age1.830677014
Gers0.85951090.5High middle age1.744372199
Gers0.9635986.5Early middle age-0.542904720
Gers1.0915858.5Early middle age-0.332805456
Gers1.2485701.5Early middle age-0.326962891
Gers1.4305519.5Early middle age0.016914150
Gers1.6885261.5End Roman period-Early Middle Age-0.065476597
Gers1.7320218.0End Roman period-Early Middle Age-0.154609860
Gers1.9665-16.5Roman period-0.214903914
Gers2.0440-94.0Iron Age-0.327259967
Gers2.2145-264.5Iron Age-0.507484772
Gers2.4960-546.0Iron Age-0.515468978
Gers2.5855-635.5Iron Age-0.455160963
Gers2.7075-757.5Mid-late Bronze age-0.627079235
Gers2.8795-929.5Mid-late Bronze age-0.613069510
Gers3.1120-1162.0Mid-late Bronze age-0.335257771
Gers3.2775-1327.5Mid-late Bronze age-0.466080457
Gers3.5535-1603.5Mid-late Bronze age-0.427616825
Gers3.7605-1810.5Early Bronze age-0.605437902
Gers3.9780-2028.0Early Bronze age-0.575985431
Gers4.1495-2199.5Early Bronze age-0.462079063
Gers4.2260-2276.0Neolithic-0.605277603
Gers4.3050-2355.0Neolithic-0.587009328
Gers4.4140-2464.0Neolithic-0.506644063
Gers4.5570-2607.0Neolithic-0.396865770