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Data Publisher for Earth & Environmental Science

Giguet-Covex, Charline (2023): Nature of agricultural and pastoral activities and main trees in Lake La Thuile catchment over the last 6400 years [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.958842, In: Giguet-Covex, Charline; Chen, Wentao; Ficetola, Francesco Gentile; Gielly, Ludovic; Pansu, Johan (2023): Plant DNA analyses, nature and intensity of human activities, and erosion intensities in the North-western Alps over the last 4000-6000 years inferred from lake sediments [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.958732

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Keyword(s):
Agriculture; DNA metabarcoding; lake sediment DNA; lake sediments; pastoralism; tree cover
Related to:
Giguet-Covex, Charline; Bajard, Manon; Chen, Wentao; Walsh, Kevin; Rey, Pierre-Jérôme; Messager, Erwan; Etienne, David; Sabatier, Pierre; Ficetola, Francesco Gentile; Gielly, Ludovic; Blanchet, C; Guffond, Christophe; Chiquet, Patricia; Arnaud, Fabien; Poulenard, Jérôme (2023): Long-term trajectories of mountain agro-ecosystems in the North-Western Alps. Regional Environmental Change, 23(2), 58, https://doi.org/10.1007/s10113-023-02030-5
Coverage:
Latitude: 45.530587 * Longitude: 6.056265
Date/Time Start: 2010-04-26T00:00:00 * Date/Time End: 2010-04-26T00:00:00
Minimum Elevation: 874.0 m * Maximum Elevation: 874.0 m
Event(s):
THU10_I * Latitude: 45.530587 * Longitude: 6.056265 * Date/Time: 2010-04-26T00:00:00 * Elevation: 874.0 m * Location: North-western Alps * Method/Device: Sediment corer (SEDCO)
Comment:
Method used to produce the sedimentary DNA datasets: DNA sequences were produced by using g (5'-GGGCAATCCTGAGCCAA-3') and h (5'-CCATTGAGTCTCTGCACCTATC-3') primers for plants and MamP007F (5'-CGAGAAGACCCTATGGAGCT-3') and MamP007R (5'-CCGAGGTCRCCCCAACC-3') primers and the human-blocking primer (5'-GGAGCTTTAATTTATTAATGCAAACAGTACCC-3') for mammals. The sequences have been produced by the Illumina technology (HiSeq instrument). The bioinformatic treatment of the sequences was operated applying the Obitool program (Boyer, F. et al. OBITOOLS:aUNIX-inspired software package for DNA metabarcoding. Mol. Ecol. Resour. 16, 176–182 (2016)) and following the procedure described in Giguet-Covex et al. New insights on lake sediment DNA from the catchment: importance of taphonomic and analytical issues on the record quality. Scientific Reports. 9, 14676 (2019) (DOI https://doi.org/10.1038/s41598-019-50339-1). Then, other filtering processing were performed as also described in Giguet-Covex et al. 2019. These second treatments allow for removing 1) taxa assigned to a reference taxon at <95 % of sequence identity, 2) potential contaminations (based on sampling, extraction and PCR controls, and recognition of exotic taxa) and 3) stochastic detections. Several analytical replicates by samples were performed (4 for the plants and 12 for the mammals). Data are presented in number of positive replicates by sample. A replicate is considered as positive if more than 5 sequences are detected.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
LakeLakeGiguet-Covex, Charline
AGEAgeka BPGiguet-Covex, CharlineAge model, radiocarbon dates and short radionuclidesGeocode
AgeAgea AD/CEGiguet-Covex, CharlineAge model, radiocarbon dates and short radionuclides
Period, culturalPeriod culturalGiguet-Covex, Charline
BosBos#Giguet-Covex, CharlineDNA metabarcoding approachNumber of positive replicates over 12
OvisOvis#Giguet-Covex, CharlineDNA metabarcoding approachNumber of positive replicates over 12
AbiesAbi#Giguet-Covex, CharlineDNA metabarcoding approachNumber of positive replicates over 4
FagusFag#Giguet-Covex, CharlineDNA metabarcoding approachNumber of positive replicates over 4
PiceaPic#Giguet-Covex, CharlineDNA metabarcoding approachNumber of positive replicates over 4
10 AlnusAln#Giguet-Covex, CharlineDNA metabarcoding approachNumber of positive replicates over 4
11 PlantagoPla#Giguet-Covex, CharlineDNA metabarcoding approachNumber of positive replicates over 4
12 RumexRum#Giguet-Covex, CharlineDNA metabarcoding approachNumber of positive replicates over 4
13 Cannabis sativaCan.s#Giguet-Covex, CharlineDNA metabarcoding approachNumber of positive replicates over 4
14 Vicia fabaVic.f#Giguet-Covex, CharlineDNA metabarcoding approachNumber of positive replicates over 4
15 JuglandaceaeJugae#Giguet-Covex, CharlineDNA metabarcoding approachNumber of positive replicates over 4
16 PrunusPrn#Giguet-Covex, CharlineDNA metabarcoding approachNumber of positive replicates over 4
17 PyrusYus#Giguet-Covex, CharlineDNA metabarcoding approachNumber of positive replicates over 4
18 MaleaeMaleae#Giguet-Covex, CharlineDNA metabarcoding approachNumber of positive replicates over 4
19 VitaceaeViteae#Giguet-Covex, CharlineDNA metabarcoding approachNumber of positive replicates over 4
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
722 data points

Data

Download dataset as tab-delimited text — use the following character encoding:


Lake

Age [ka BP]

Age [a AD/CE]

Period cultural

Bos [#]

Ovis [#]

Abi [#]

Fag [#]

Pic [#]
10 
Aln [#]
11 
Pla [#]
12 
Rum [#]
13 
Can.s [#]
14 
Vic.f [#]
15 
Jugae [#]
16 
Prn [#]
17 
Yus [#]
18 
Maleae [#]
19 
Viteae [#]
La Thuile-0.0011951Contemporary000201110000000
La Thuile0.1291821Late modern200102010001100
La Thuile0.2601690Early modern100000100001100
La Thuile0.3891561Early modern001101020022110
La Thuile0.5201430Late middle Age500011200130411
La Thuile0.6491301Late middle Age101100311013121
La Thuile0.7801170High middle Age210420010032213
La Thuile0.9101040High middle Age101001330100210
La Thuile1.039911High middle Age030000303200320
La Thuile1.170780Early middle Age000000103000010
La Thuile1.284666Early middle Age000100002000000
La Thuile1.430520Early middle Age001402301100010
La Thuile1.557393End Roman period-Early Middle Age004304100000020
La Thuile1.694256End Roman period-Early Middle Age101413000001000
La Thuile1.821129Roman period002434110001010
La Thuile1.9473Roman period000404010000010
La Thuile2.085-135Iron Age000403000000010
La Thuile2.212-262Iron Age000404010001120
La Thuile2.338-388Iron Age000114200000000
La Thuile2.465-515Iron Age004404000002010
La Thuile3.862-1912Early Bronze Age001001000000000
La Thuile3.946-1996Early Bronze Age001101010000000
La Thuile4.030-2080Early Bronze Age001021010000000
La Thuile4.160-2210Neolithic001101000000000
La Thuile4.290-2340Neolithic002302000000010
La Thuile4.420-2470Neolithic101112000000000
La Thuile4.550-2600Neolithic011004000000000
La Thuile4.680-2730Neolithic000002000000010
La Thuile4.810-2860Neolithic101004000000000
La Thuile4.940-2990Neolithic103004000000000
La Thuile5.075-3125Neolithic003002000000000
La Thuile5.200-3250Neolithic001004000000000
La Thuile5.330-3380Neolithic101004000000010
La Thuile5.460-3510Pre anthropic2003000000010
La Thuile5.590-3640Pre anthropic1003010000020
La Thuile5.720-3770Pre anthropic2004000000010
La Thuile5.850-3900Pre anthropic1001010000000
La Thuile5.980-4030Pre anthropic1003000000040
La Thuile6.110-4160Pre anthropic2004000000010
La Thuile6.240-4290Pre anthropic2013000000000
La Thuile6.370-4420Pre anthropic0002000000000