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Data Publisher for Earth & Environmental Science

Giguet-Covex, Charline; Chen, Wentao; Ficetola, Francesco Gentile; Bajard, Manon (2023): Nature of pastoral activities in Lake Verney catchment over the last 4600 years from sedimentary DNA and Sporormiella spores [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.958764, In: Giguet-Covex, Charline; Chen, Wentao; Ficetola, Francesco Gentile; Gielly, Ludovic; Pansu, Johan (2023): Plant DNA analyses, nature and intensity of human activities, and erosion intensities in the North-western Alps over the last 4000-6000 years inferred from lake sediments [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.958732

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Keyword(s):
DNA metabarcoding; lake sediment DNA; lake sediments; pastoralism; Sporormiella spore concentration
Related to:
Giguet-Covex, Charline; Bajard, Manon; Chen, Wentao; Walsh, Kevin; Rey, Pierre-Jérôme; Messager, Erwan; Etienne, David; Sabatier, Pierre; Ficetola, Francesco Gentile; Gielly, Ludovic; Blanchet, C; Guffond, Christophe; Chiquet, Patricia; Arnaud, Fabien; Poulenard, Jérôme (2023): Long-term trajectories of mountain agro-ecosystems in the North-Western Alps. Regional Environmental Change, 23(2), 58, https://doi.org/10.1007/s10113-023-02030-5
Coverage:
Latitude: 45.687916 * Longitude: 6.057317
Minimum Elevation: 2090.0 m * Maximum Elevation: 2090.0 m
Event(s):
VER13_03 * Latitude: 45.687916 * Longitude: 6.057317 * Elevation: 2090.0 m * Method/Device: Sediment corer (SEDCO)
Comment:
Method used to produce the sediment DNA datasets: DNA sequences were generated using g (5'-GGGCAATCCTGAGCCAA-3') and h (5'-CCATTGAGTCTCTGCACCTATC-3') primers for plants and MamP007F (5'-CGAGAAGACCCTATGGAGCT-3') and MamP007R (5'-CCGAGGTCRCCCCAACC-3') primers and the human-blocking primer (5'-GGAGCTTTAATTTATTAATGCAAACAGTACCC-3') for mammals. The sequences have been produced by the Illumina technology (HiSeq instrument). The bioinformatic treatment of the sequences was operated applying the Obitool program (Boyer, F. et al. OBITOOLS:aUNIX-inspired software package for DNA metabarcoding. Mol. Ecol. Resour. 16, 176–182 (2016)) and following the procedure described in Giguet-Covex et al. New insights on lake sediment DNA from the catchment: importance of taphonomic and analytical issues on the record quality. Scientific Reports. 9, 14676 (2019) (DOI https://doi.org/10.1038/s41598-019-50339-1). Further filtering treatments were then applied, as also described in Giguet-Covex et al. 2019. These second treatments allow the removal of 1) taxa assigned to a reference taxon at <95 % of sequence identity, 2) potential contaminations (based on sampling, extraction and PCR controls, and recognition of exotic taxa) and 3) stochastic detections. Several analytical replicates by samples were performed (12 for both, the plants and mammals). Data are presented in number of positive replicates by sample. A replicate is considered as positive if more than 5 sequences are detected.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
LakeLakeGiguet-Covex, Charline
AGEAgeka BPGiguet-Covex, CharlineAge model, radiocarbon dates and short radionuclidesGeocode
AgeAgea AD/CEGiguet-Covex, CharlineAge model, radiocarbon dates and short radionuclides
Period, culturalPeriod culturalGiguet-Covex, Charline
BosBos#Giguet-Covex, CharlineDNA metabarcoding approachNumber of positive replicates over 12
OvisOvis#Giguet-Covex, CharlineDNA metabarcoding approachNumber of positive replicates over 12
PiceaPic#Giguet-Covex, Charline
PinusPin#Giguet-Covex, Charline
AlnusAln#Giguet-Covex, Charline
10 PlantagoPla#Giguet-Covex, CharlineDNA metabarcoding approachNumber of positive replicates over 12
11 RumexRum#Giguet-Covex, CharlineDNA metabarcoding approachNumber of positive replicates over 12
12 SporormiellaSporormiella#/cm3Giguet-Covex, Charline
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
462 data points

Data

Download dataset as tab-delimited text — use the following character encoding:


Lake

Age [ka BP]

Age [a AD/CE]

Period cultural

Bos [#]

Ovis [#]

Pic [#]

Pin [#]

Aln [#]
10 
Pla [#]
11 
Rum [#]
12 
Sporormiella [#/cm3]
Verney-0.0532003Contemporary0014090694.2314548
Verney-0.0401990Contemporary00001511023.4682830
Verney-0.0251975Contemporary00101120836.1392208
Verney-0.0061956Contemporary000001011063.4552730
Verney0.0181932Late modern1010070951.3062400
Verney0.0461904Late modern1010081827.3030075
Verney0.0741876Late modern00100801030.2728570
Verney0.1051845Late modern0001091507.5176471
Verney0.1501800Late modern00001120821.0133333
Verney0.2051745Early modern00120811944.7071430
Verney0.2761674Early modern01010902034.9473680
Verney0.3441606Early modern103001012712.5384620
Verney0.3961554Early modern000001001188.6208070
Verney0.4531497Early modern21000911402.4875230
Verney0.5111439Late middle age11001701997.7525100
Verney0.5651385Late middle age10000721155.4105770
Verney0.6191331Late middle age01100601325.2907040
Verney0.6721278Late middle age001101012789.8729410
Verney0.7221228High middle age112001021728.8465570
Verney0.7701180High middle age31000914115.4853850
Verney0.8141136High middle age00002901277.2707990
Verney0.8531097High middle age02440432224.3733330
Verney0.9021048High middle age00041321657.9284460
Verney0.960990Early middle age0031003996.7072131
Verney1.057893Early middle age01320321925.5252690
Verney1.161789Early middle age00020511109.1994840
Verney1.283667Early middle age00100441803.9104430
Verney1.439511Early middle age02320410949.2406531
Verney1.589361End Roman period-Early Middle Age0000039613.1184466
Verney1.757193Roman period10100611493.5725709
Verney1.962-12Roman period00401471105.7133290
Verney2.210-260Iron Age00010781911.0810070
Verney2.612-662Iron Age03112761931.1477710
Verney3.038-1088Mid-Late Bronze Age00310723105.8986110
Verney3.352-1402Mid-Late Bronze Age00100112131.5831430
Verney3.568-1618Mid-Late Bronze Age00010231114.1883870
Verney3.724-1774Early Bronze age00160022231.7027100
Verney3.908-1958Early Bronze age0001001410.1596439
Verney4.094-2144Early Bronze age00050112188.4589040
Verney4.281-2331Neolithic0013012296.1369474
Verney4.452-2502Neolithic0005000543.7376183
Verney4.635-2685Neolithic0011000135.7776065