Citation:

PANGAEA.958748

Name: Seawater carbonate chemistry and genes regulating neurogenesis and neuroplasticity in brains of three-spined stickleback, cinnamon anemonefish and spiny damselfish exposed to elevated CO2
Published: 2023-05-10
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Acanthochromis polyacanthus; Amphiprion melanopus; Animalia; Chordata; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Gasterosteus aculeatus; Gene expression (incl. proteomics); Laboratory experiment; Nekton; North Atlantic; Pelagos; Single species; South Pacific; Temperate; Tropical

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Abstract:

The continuous increase of anthropogenic CO2 in the atmosphere resulting in ocean acidification has been reported to affect brain function in some fishes. During adulthood, cell proliferation is fundamental for fish brain growth and for it to adapt in response to external stimuli, such as environmental changes. Here we report the first expression study of genes regulating neurogenesis and neuroplasticity in brains of three-spined stickleback (Gasterosteus aculeatus), cinnamon anemonefish (Amphiprion melanopus) and spiny damselfish (Acanthochromis polyacanthus) exposed to elevated CO2. The mRNA expression levels of the neurogenic differentiation factor (NeuroD) and doublecortin (DCX) were upregulated in three-spined stickleback exposed to high-CO2 compared with controls, while no changes were detected in the other species. The mRNA expression levels of the proliferating cell nuclear antigen (PCNA) and the brain-derived neurotrophic factor (BDNF) remained unaffected in the high-CO2 exposed groups compared to the control in all three species. These results indicate a species-specific regulation of genes involved in neurogenesis in response to elevated ambient CO2 levels. The higher expression of NeuroD and DCX mRNA transcripts in the brain of high-CO2–exposed three-spined stickleback, together with the lack of effects on mRNA levels in cinnamon anemonefish and spiny damselfish, indicate differences in coping mechanisms among fish in response to the predicted-future CO2 level.

Keyword(s):

Acanthochromis polyacanthus; Amphiprion melanopus; Animalia; Chordata; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Gasterosteus aculeatus; Gene expression (incl. proteomics); Laboratory experiment; Nekton; North Atlantic; Pelagos; Single species; South Pacific; Temperate; Tropical

Supplement to:

Lai, Floriana; Fagernes, Cathrine E; Bernier, Nicholas J; Miller, Garielle M; Munday, Philip L; Jutfelt, Fredrik; Nilsson, Göran E (2017): Responses of neurogenesis and neuroplasticity related genes to elevated CO 2 levels in the brain of three teleost species. Biology Letters, 13(8), 20170240, https://doi.org/10.1098/rsbl.2017.0240

Source:

Lai, Floriana; Fagernes, Cathrine E; Bernier, Nicholas J; Miller, Garielle M; Munday, Philip L; Jutfelt, Fredrik; Nilsson, Göran E (2017): Data from: Responses of neurogenesis and neuroplasticity related genes to elevated CO2 levels in the brain of three teleost species. Dryad, https://doi.org/10.5061/dryad.pn397

Documentation:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2022): seacarb: seawater carbonate chemistry with R. R package version 3.3.1. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-05-08.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type of study	Study type		Lai, Floriana
2	Species, unique identification	Species UID		Lai, Floriana
3	Species, unique identification (URI)	Species UID (URI)		Lai, Floriana
4	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		Lai, Floriana
5	Individual ID	Ind ID		Lai, Floriana
6	Treatment	Treat		Lai, Floriana
7	mRNA gene expression, relative	mRNA express		Lai, Floriana		PCNA (ubc/rpl13A)
8	mRNA gene expression, relative	mRNA express		Lai, Floriana		NeuroD (ubc/rpl13A)
9	mRNA gene expression, relative	mRNA express		Lai, Floriana		DCX (ubc/rpl13A)
10	mRNA gene expression, relative	mRNA express		Lai, Floriana		BDNF (ubc/rpl13A)
11	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Lai, Floriana
12	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Lai, Floriana
13	Alkalinity, total	AT	µmol/kg	Lai, Floriana
14	Alkalinity, total, standard deviation	AT std dev	±	Lai, Floriana
15	Salinity	Sal		Lai, Floriana
16	Salinity, standard deviation	Sal std dev	±	Lai, Floriana
17	Temperature, water	Temp	°C	Lai, Floriana
18	Temperature, water, standard deviation	Temp std dev	±	Lai, Floriana
19	pH	pH		Lai, Floriana		total scale
20	pH, standard deviation	pH std dev	±	Lai, Floriana
21	Aragonite saturation state	Omega Arg		Lai, Floriana
22	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Lai, Floriana
23	Calcite saturation state	Omega Cal		Lai, Floriana
24	Calcite saturation state, standard deviation	Omega Cal std dev	±	Lai, Floriana
25	pH	pH		Lai, Floriana		NBS scale
26	pH, standard deviation	pH std dev	±	Lai, Floriana
27	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
28	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
29	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
31	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
32	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
33	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1474 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Study type	2 Species UID	3 Species UID (URI)	4 Species UID (Semantic URI)	5 Ind ID	6 Treat	7 mRNA express (PCNA (ubc/rpl13A))	8 mRNA express (NeuroD (ubc/rpl13A))	9 mRNA express (DCX (ubc/rpl13A))	10 mRNA express (BDNF (ubc/rpl13A))	11 pCO2water_SST_wet [µatm]	12 pCO2 std dev [±]	13 AT [µmol/kg]	14 AT std dev [±]	15 Sal	16 Sal std dev [±]	17 Temp [°C]	18 Temp std dev [±]	19 pH (total scale)	20 pH std dev [±]	21 Omega Arg	22 Omega Arg std dev [±]	23 Omega Cal	24 Omega Cal std dev [±]	25 pH (NBS scale)	26 pH std dev [±]	27 CSC flag	28 pH	29 CO2 [µmol/kg]	30 fCO2water_SST_wet [µatm]	31 pCO2water_SST_wet [µatm]	32 [HCO3]- [µmol/kg]	33 [CO3]2- [µmol/kg]	34 DIC [µmol/kg]	35 Omega Arg	36 Omega Cal
laboratory	Gasterosteus aculeatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:126505	1	Control	0.021720	0.074730	0.028660	0.013680	333.0	33.0	1890	386	24.20	3.40	17.6	1.20	8.08	0.085	2.09	0.74	3.34	1.19			24	8.08	12.21	331.84		1597.07	119.82	1729.11	1.91	3.07
laboratory	Gasterosteus aculeatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:126505	2	Control	0.017890	0.084770	0.043950	0.009950	333.0	33.0	1890	386	24.20	3.40	17.6	1.20	8.08	0.085	2.09	0.74	3.34	1.19			24	8.08	12.21	331.84		1597.07	119.82	1729.11	1.91	3.07
laboratory	Gasterosteus aculeatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:126505	3	Control					333.0	33.0	1890	386	24.20	3.40	17.6	1.20	8.08	0.085	2.09	0.74	3.34	1.19			24	8.08	12.21	331.84		1597.07	119.82	1729.11	1.91	3.07
laboratory	Gasterosteus aculeatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:126505	4	Control	0.020790	0.090940	0.037660	0.007400	333.0	33.0	1890	386	24.20	3.40	17.6	1.20	8.08	0.085	2.09	0.74	3.34	1.19			24	8.08	12.21	331.84		1597.07	119.82	1729.11	1.91	3.07
laboratory	Gasterosteus aculeatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:126505	5	Control	0.020060	0.088770	0.022310	0.015430	333.0	33.0	1890	386	24.20	3.40	17.6	1.20	8.08	0.085	2.09	0.74	3.34	1.19			24	8.08	12.21	331.84		1597.07	119.82	1729.11	1.91	3.07
laboratory	Gasterosteus aculeatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:126505	6	Control	0.022760	0.074430	0.037440	0.008880	333.0	33.0	1890	386	24.20	3.40	17.6	1.20	8.08	0.085	2.09	0.74	3.34	1.19			24	8.08	12.21	331.84		1597.07	119.82	1729.11	1.91	3.07
laboratory	Gasterosteus aculeatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:126505	7	Control	0.021710			0.011400	333.0	33.0	1890	386	24.20	3.40	17.6	1.20	8.08	0.085	2.09	0.74	3.34	1.19			24	8.08	12.21	331.84		1597.07	119.82	1729.11	1.91	3.07
laboratory	Gasterosteus aculeatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:126505	8	Control	0.017190	0.041300	0.042770	0.009720	333.0	33.0	1890	386	24.20	3.40	17.6	1.20	8.08	0.085	2.09	0.74	3.34	1.19			24	8.08	12.21	331.84		1597.07	119.82	1729.11	1.91	3.07
laboratory	Gasterosteus aculeatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:126505	9	Control	0.013310	0.052910	0.023820	0.009440	333.0	33.0	1890	386	24.20	3.40	17.6	1.20	8.08	0.085	2.09	0.74	3.34	1.19			24	8.08	12.21	331.84		1597.07	119.82	1729.11	1.91	3.07
laboratory	Gasterosteus aculeatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:126505	10	Control	0.017160	0.080430	0.007030	0.009190	333.0	33.0	1890	386	24.20	3.40	17.6	1.20	8.08	0.085	2.09	0.74	3.34	1.19			24	8.08	12.21	331.84		1597.07	119.82	1729.11	1.91	3.07
laboratory	Gasterosteus aculeatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:126505	11	Control	0.026940			0.012940	333.0	33.0	1890	386	24.20	3.40	17.6	1.20	8.08	0.085	2.09	0.74	3.34	1.19			24	8.08	12.21	331.84		1597.07	119.82	1729.11	1.91	3.07
laboratory	Gasterosteus aculeatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:126505	12	Control	0.023430	0.105330	0.031750	0.014690	333.0	33.0	1890	386	24.20	3.40	17.6	1.20	8.08	0.085	2.09	0.74	3.34	1.19			24	8.08	12.21	331.84		1597.07	119.82	1729.11	1.91	3.07
laboratory	Gasterosteus aculeatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:126505	1	High CO2	0.015840	0.102670	0.043720	0.009680	991.3	56.6	1839	131	24.20	3.40	17.6	1.20	7.65	0.031	0.82	0.11	1.31	0.18			24	7.64	36.36	987.83		1723.64	46.89	1806.89	0.75	1.20
laboratory	Gasterosteus aculeatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:126505	2	High CO2	0.023187	0.102740	0.068350	0.015800	991.3	56.6	1839	131	24.20	3.40	17.6	1.20	7.65	0.031	0.82	0.11	1.31	0.18			24	7.64	36.36	987.83		1723.64	46.89	1806.89	0.75	1.20
laboratory	Gasterosteus aculeatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:126505	3	High CO2					991.3	56.6	1839	131	24.20	3.40	17.6	1.20	7.65	0.031	0.82	0.11	1.31	0.18			24	7.64	36.36	987.83		1723.64	46.89	1806.89	0.75	1.20
laboratory	Gasterosteus aculeatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:126505	4	High CO2	0.016030	0.094610	0.050020	0.011590	991.3	56.6	1839	131	24.20	3.40	17.6	1.20	7.65	0.031	0.82	0.11	1.31	0.18			24	7.64	36.36	987.83		1723.64	46.89	1806.89	0.75	1.20
laboratory	Gasterosteus aculeatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:126505	5	High CO2	0.017907		0.037520	0.008410	991.3	56.6	1839	131	24.20	3.40	17.6	1.20	7.65	0.031	0.82	0.11	1.31	0.18			24	7.64	36.36	987.83		1723.64	46.89	1806.89	0.75	1.20
laboratory	Gasterosteus aculeatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:126505	6	High CO2	0.016715	0.078350	0.039740	0.011280	991.3	56.6	1839	131	24.20	3.40	17.6	1.20	7.65	0.031	0.82	0.11	1.31	0.18			24	7.64	36.36	987.83		1723.64	46.89	1806.89	0.75	1.20
laboratory	Gasterosteus aculeatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:126505	7	High CO2	0.018704	0.097300		0.015210	991.3	56.6	1839	131	24.20	3.40	17.6	1.20	7.65	0.031	0.82	0.11	1.31	0.18			24	7.64	36.36	987.83		1723.64	46.89	1806.89	0.75	1.20
laboratory	Gasterosteus aculeatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:126505	8	High CO2	0.011442		0.032110	0.013900	991.3	56.6	1839	131	24.20	3.40	17.6	1.20	7.65	0.031	0.82	0.11	1.31	0.18			24	7.64	36.36	987.83		1723.64	46.89	1806.89	0.75	1.20
laboratory	Gasterosteus aculeatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:126505	9	High CO2	0.029679	0.103750	0.062300	0.010990	991.3	56.6	1839	131	24.20	3.40	17.6	1.20	7.65	0.031	0.82	0.11	1.31	0.18			24	7.64	36.36	987.83		1723.64	46.89	1806.89	0.75	1.20
laboratory	Gasterosteus aculeatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:126505	10	High CO2	0.024363	0.100010	0.049130	0.011150	991.3	56.6	1839	131	24.20	3.40	17.6	1.20	7.65	0.031	0.82	0.11	1.31	0.18			24	7.64	36.36	987.83		1723.64	46.89	1806.89	0.75	1.20
laboratory	Gasterosteus aculeatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:126505	11	High CO2	0.018237	0.117810			991.3	56.6	1839	131	24.20	3.40	17.6	1.20	7.65	0.031	0.82	0.11	1.31	0.18			24	7.64	36.36	987.83		1723.64	46.89	1806.89	0.75	1.20
laboratory	Gasterosteus aculeatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:126505	12	High CO2	0.022735	0.093230	0.034480	0.012080	991.3	56.6	1839	131	24.20	3.40	17.6	1.20	7.65	0.031	0.82	0.11	1.31	0.18			24	7.64	36.36	987.83		1723.64	46.89	1806.89	0.75	1.20
laboratory	Amphiprion melanopus	marinespecies.org	urn:lsid:marinespecies.org:taxname:278398	1	Control	0.111294	0.323206	0.232672	0.076814			2058	16	33.32	0.12	28.4	0.01							8.15	0.005	26	8.00	10.55	400.20	401.43	1588.36	188.49	1787.40	3.08	4.63
laboratory	Amphiprion melanopus	marinespecies.org	urn:lsid:marinespecies.org:taxname:278398	2	Control	0.092887	0.254169	0.299707	0.061481			2058	16	33.32	0.12	28.4	0.01							8.15	0.005	26	8.00	10.55	400.20	401.43	1588.36	188.49	1787.40	3.08	4.63
laboratory	Amphiprion melanopus	marinespecies.org	urn:lsid:marinespecies.org:taxname:278398	3	Control	0.075178	0.200122	0.254004	0.074326			2058	16	33.32	0.12	28.4	0.01							8.15	0.005	26	8.00	10.55	400.20	401.43	1588.36	188.49	1787.40	3.08	4.63
laboratory	Amphiprion melanopus	marinespecies.org	urn:lsid:marinespecies.org:taxname:278398	4	Control	0.043095	0.357713	0.206966	0.086702			2058	16	33.32	0.12	28.4	0.01							8.15	0.005	26	8.00	10.55	400.20	401.43	1588.36	188.49	1787.40	3.08	4.63
laboratory	Amphiprion melanopus	marinespecies.org	urn:lsid:marinespecies.org:taxname:278398	5	Control	0.072555	0.252517	0.158531	0.043072			2058	16	33.32	0.12	28.4	0.01							8.15	0.005	26	8.00	10.55	400.20	401.43	1588.36	188.49	1787.40	3.08	4.63
laboratory	Amphiprion melanopus	marinespecies.org	urn:lsid:marinespecies.org:taxname:278398	6	Control	0.071974	0.341639	0.246080	0.078923			2058	16	33.32	0.12	28.4	0.01							8.15	0.005	26	8.00	10.55	400.20	401.43	1588.36	188.49	1787.40	3.08	4.63
laboratory	Amphiprion melanopus	marinespecies.org	urn:lsid:marinespecies.org:taxname:278398	7	Control	0.063040	0.244663	0.155291	0.043537			2058	16	33.32	0.12	28.4	0.01							8.15	0.005	26	8.00	10.55	400.20	401.43	1588.36	188.49	1787.40	3.08	4.63
laboratory	Amphiprion melanopus	marinespecies.org	urn:lsid:marinespecies.org:taxname:278398	8	Control	0.070540	0.211116	0.239905	0.028574			2058	16	33.32	0.12	28.4	0.01							8.15	0.005	26	8.00	10.55	400.20	401.43	1588.36	188.49	1787.40	3.08	4.63
laboratory	Amphiprion melanopus	marinespecies.org	urn:lsid:marinespecies.org:taxname:278398	9	Control	0.061426	0.417838	0.127530	0.060115			2058	16	33.32	0.12	28.4	0.01							8.15	0.005	26	8.00	10.55	400.20	401.43	1588.36	188.49	1787.40	3.08	4.63
laboratory	Amphiprion melanopus	marinespecies.org	urn:lsid:marinespecies.org:taxname:278398	10	Control	0.063119	0.260821	0.209900	0.049707			2058	16	33.32	0.12	28.4	0.01							8.15	0.005	26	8.00	10.55	400.20	401.43	1588.36	188.49	1787.40	3.08	4.63
laboratory	Amphiprion melanopus	marinespecies.org	urn:lsid:marinespecies.org:taxname:278398	11	Control	0.064464	0.344861	0.279769	0.066024			2058	16	33.32	0.12	28.4	0.01							8.15	0.005	26	8.00	10.55	400.20	401.43	1588.36	188.49	1787.40	3.08	4.63
laboratory	Amphiprion melanopus	marinespecies.org	urn:lsid:marinespecies.org:taxname:278398	12	Control	0.050389	0.162344	0.092796	0.049770			2058	16	33.32	0.12	28.4	0.01							8.15	0.005	26	8.00	10.55	400.20	401.43	1588.36	188.49	1787.40	3.08	4.63
laboratory	Amphiprion melanopus	marinespecies.org	urn:lsid:marinespecies.org:taxname:278398	1	High CO2	0.096409	0.279535	0.356223	0.074907			2168	7	33.62	0.09	28.5	0.01							7.81	0.008	26	7.66	27.62	1052.16	1055.40	1910.32	104.76	2042.70	1.71	2.57
laboratory	Amphiprion melanopus	marinespecies.org	urn:lsid:marinespecies.org:taxname:278398	2	High CO2	0.080576	0.268487	0.270570	0.088327			2168	7	33.62	0.09	28.5	0.01							7.81	0.008	26	7.66	27.62	1052.16	1055.40	1910.32	104.76	2042.70	1.71	2.57
laboratory	Amphiprion melanopus	marinespecies.org	urn:lsid:marinespecies.org:taxname:278398	3	High CO2	0.077424	0.254114	0.354197	0.046704			2168	7	33.62	0.09	28.5	0.01							7.81	0.008	26	7.66	27.62	1052.16	1055.40	1910.32	104.76	2042.70	1.71	2.57
laboratory	Amphiprion melanopus	marinespecies.org	urn:lsid:marinespecies.org:taxname:278398	4	High CO2	0.063484	0.432852	0.140386	0.079100			2168	7	33.62	0.09	28.5	0.01							7.81	0.008	26	7.66	27.62	1052.16	1055.40	1910.32	104.76	2042.70	1.71	2.57
laboratory	Amphiprion melanopus	marinespecies.org	urn:lsid:marinespecies.org:taxname:278398	5	High CO2	0.044218		0.154955				2168	7	33.62	0.09	28.5	0.01							7.81	0.008	26	7.66	27.62	1052.16	1055.40	1910.32	104.76	2042.70	1.71	2.57
laboratory	Amphiprion melanopus	marinespecies.org	urn:lsid:marinespecies.org:taxname:278398	6	High CO2	0.057228	0.281262	0.275670	0.044096			2168	7	33.62	0.09	28.5	0.01							7.81	0.008	26	7.66	27.62	1052.16	1055.40	1910.32	104.76	2042.70	1.71	2.57
laboratory	Amphiprion melanopus	marinespecies.org	urn:lsid:marinespecies.org:taxname:278398	7	High CO2	0.069004	0.242787	0.183551	0.039957			2168	7	33.62	0.09	28.5	0.01							7.81	0.008	26	7.66	27.62	1052.16	1055.40	1910.32	104.76	2042.70	1.71	2.57
laboratory	Amphiprion melanopus	marinespecies.org	urn:lsid:marinespecies.org:taxname:278398	8	High CO2	0.059993	0.242212	0.207001	0.041566			2168	7	33.62	0.09	28.5	0.01							7.81	0.008	26	7.66	27.62	1052.16	1055.40	1910.32	104.76	2042.70	1.71	2.57
laboratory	Amphiprion melanopus	marinespecies.org	urn:lsid:marinespecies.org:taxname:278398	9	High CO2	0.067225	0.277352	0.138663	0.039011			2168	7	33.62	0.09	28.5	0.01							7.81	0.008	26	7.66	27.62	1052.16	1055.40	1910.32	104.76	2042.70	1.71	2.57
laboratory	Amphiprion melanopus	marinespecies.org	urn:lsid:marinespecies.org:taxname:278398	10	High CO2	0.077630	0.365016	0.132525	0.038347			2168	7	33.62	0.09	28.5	0.01							7.81	0.008	26	7.66	27.62	1052.16	1055.40	1910.32	104.76	2042.70	1.71	2.57
laboratory	Amphiprion melanopus	marinespecies.org	urn:lsid:marinespecies.org:taxname:278398	11	High CO2	0.059946	0.199307	0.172115	0.034370			2168	7	33.62	0.09	28.5	0.01							7.81	0.008	26	7.66	27.62	1052.16	1055.40	1910.32	104.76	2042.70	1.71	2.57
laboratory	Amphiprion melanopus	marinespecies.org	urn:lsid:marinespecies.org:taxname:278398	12	High CO2	0.067949	0.197285	0.215953	0.041316			2168	7	33.62	0.09	28.5	0.01							7.81	0.008	26	7.66	27.62	1052.16	1055.40	1910.32	104.76	2042.70	1.71	2.57
laboratory	Acanthochromis polyacanthus	marinespecies.org	urn:lsid:marinespecies.org:taxname:279469	1	Control	0.080262		0.790239
laboratory	Acanthochromis polyacanthus	marinespecies.org	urn:lsid:marinespecies.org:taxname:279469	2	Control	0.082039	1.137487	0.867778	0.044666
laboratory	Acanthochromis polyacanthus	marinespecies.org	urn:lsid:marinespecies.org:taxname:279469	3	Control	0.074461	1.043408	0.979092	0.049268
laboratory	Acanthochromis polyacanthus	marinespecies.org	urn:lsid:marinespecies.org:taxname:279469	4	Control	0.082873	0.683294	0.841397	0.062533
laboratory	Acanthochromis polyacanthus	marinespecies.org	urn:lsid:marinespecies.org:taxname:279469	5	Control	0.074044	0.611129	0.882274	0.057876
laboratory	Acanthochromis polyacanthus	marinespecies.org	urn:lsid:marinespecies.org:taxname:279469	6	Control	0.070332	0.668655	0.716081	0.048454
laboratory	Acanthochromis polyacanthus	marinespecies.org	urn:lsid:marinespecies.org:taxname:279469	7	Control	0.089436	0.769932	0.891841	0.061506
laboratory	Acanthochromis polyacanthus	marinespecies.org	urn:lsid:marinespecies.org:taxname:279469	8	Control	0.086997	0.910458	0.837442
laboratory	Acanthochromis polyacanthus	marinespecies.org	urn:lsid:marinespecies.org:taxname:279469	1	High CO2	0.085475	0.945560	0.822350	0.055372
laboratory	Acanthochromis polyacanthus	marinespecies.org	urn:lsid:marinespecies.org:taxname:279469	2	High CO2	0.075292	0.740189	0.735970	0.056902
laboratory	Acanthochromis polyacanthus	marinespecies.org	urn:lsid:marinespecies.org:taxname:279469	3	High CO2	0.074156	0.951415	0.652518	0.051310
laboratory	Acanthochromis polyacanthus	marinespecies.org	urn:lsid:marinespecies.org:taxname:279469	4	High CO2	0.079841	0.673790	0.866254	0.050147
laboratory	Acanthochromis polyacanthus	marinespecies.org	urn:lsid:marinespecies.org:taxname:279469	5	High CO2	0.076985	0.633929	1.012694	0.054828
laboratory	Acanthochromis polyacanthus	marinespecies.org	urn:lsid:marinespecies.org:taxname:279469	6	High CO2	0.086020	0.998548	0.816500	0.057033
laboratory	Acanthochromis polyacanthus	marinespecies.org	urn:lsid:marinespecies.org:taxname:279469	7	High CO2	0.080294	0.721699	0.872165	0.065348
laboratory	Acanthochromis polyacanthus	marinespecies.org	urn:lsid:marinespecies.org:taxname:279469	8	High CO2	0.080328	0.882214	0.817761	0.049871