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Giguet-Covex, Charline (2023): Nature of pastoral activities and trees in Lake Anterne catchment over the Holocene [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.958701, In: Giguet-Covex, Charline; Chen, Wentao; Ficetola, Francesco Gentile; Gielly, Ludovic; Pansu, Johan (2023): Plant DNA analyses, nature and intensity of human activities, and erosion intensities in the North-western Alps over the last 4000-6000 years inferred from lake sediments [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.958732

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Keyword(s):
DNA metabarcoding; lake sediment DNA; lake sediments; pastoralism
Related to:
Giguet-Covex, Charline; Bajard, Manon; Chen, Wentao; Walsh, Kevin; Rey, Pierre-Jérôme; Messager, Erwan; Etienne, David; Sabatier, Pierre; Ficetola, Francesco Gentile; Gielly, Ludovic; Blanchet, C; Guffond, Christophe; Chiquet, Patricia; Arnaud, Fabien; Poulenard, Jérôme (2023): Long-term trajectories of mountain agro-ecosystems in the North-Western Alps. Regional Environmental Change, 23(2), 58, https://doi.org/10.1007/s10113-023-02030-5
Coverage:
Latitude: 45.991133 * Longitude: 6.797783
Date/Time Start: 2007-09-05T00:00:00 * Date/Time End: 2007-09-15T00:00:00
Minimum Elevation: 2063.0 m * Maximum Elevation: 2063.0 m
Event(s):
ANT07 * Latitude: 45.991133 * Longitude: 6.797783 * Date/Time Start: 2007-09-05T00:00:00 * Date/Time End: 2007-09-15T00:00:00 * Elevation: 2063.0 m * Method/Device: Sediment corer (SEDCO)
Comment:
Method used to produce the sediment DNA datasets: DNA sequences were generated using g (5'-GGGCAATCCTGAGCCAA-3') and h (5'-CCATTGAGTCTCTGCACCTATC-3') primers for plants and MamP007F (5'-CGAGAAGACCCTATGGAGCT-3') and MamP007R (5'-CCGAGGTCRCCCCAACC-3') primers and the human-blocking primer (5'-GGAGCTTTAATTTATTAATGCAAACAGTACCC-3') for mammals. The sequences have been produced by the Illumina technology (HiSeq instrument). The bioinformatic treatment of the sequences was operated applying the Obitool program (Boyer, F. et al. OBITOOLS:aUNIX-inspired software package for DNAmetabarcoding. Mol. Ecol. Resour. 16, 176–182 (2016)) and following the procedure described in Giguet-Covex et al. New insights on lake sediment DNA from the catchment: importance of taphonomic and analytical issues on the record quality. Scientific Reports. 9, 14676 (2019) (DOI https://doi.org/10.1038/s41598-019-50339-1). Further filtering treatments were then applied, as also described in Giguet-Covex et al. 2019. These second treatments allow the removal of 1) taxa assigned to a reference taxon at <95 % of sequence identity, 2) potential contaminations (based on sampling, extraction and PCR controls, and recognition of exotic taxa) and 3) stochastic detections. Several analytical replicates by samples were performed (8 for both, the plants and mammals). Data are presented in number of positive replicates by sample. A replicate is considered as positive if more than 5 sequences are detected.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
LakeLakeGiguet-Covex, Charline
AGEAgeka BPGiguet-Covex, CharlineAge model, radiocarbon dates and short radionuclidesGeocode
AgeAgea AD/CEGiguet-Covex, CharlineAge model, radiocarbon dates and short radionuclides
Period, culturalPeriod culturalGiguet-Covex, Charline
BosBos#Giguet-Covex, CharlineDNA metabarcoding approachNumber of positive replicates over 8
OvisOvis#Giguet-Covex, CharlineDNA metabarcoding approachNumber of positive replicates over 8
EquusEquus#Giguet-Covex, CharlineDNA metabarcoding approachNumber of positive replicates over 8
PinusPin#Giguet-Covex, CharlineDNA metabarcoding approachNumber of positive replicates over 8
PiceaPic#Giguet-Covex, CharlineDNA metabarcoding approachNumber of positive replicates over 8
10 AlnusAln#Giguet-Covex, CharlineDNA metabarcoding approachNumber of positive replicates over 8
11 PlantagoPla#Giguet-Covex, CharlineDNA metabarcoding approachNumber of positive replicates over 8
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
440 data points

Data

Download dataset as tab-delimited text — use the following character encoding:


Lake

Age [ka BP]

Age [a AD/CE]

Period cultural

Bos [#]

Ovis [#]

Equus [#]

Pin [#]

Pic [#]
10 
Aln [#]
11 
Pla [#]
Anterne-0.056002006.00Contemporary1001003
Anterne0.055001895.00Late Modern0000003
Anterne0.170991779.01Late Modern2000105
Anterne0.343431606.57Early Modern1000207
Anterne0.445551504.45Early Modern4000005
Anterne0.521071428.93Late Middle Age5000008
Anterne0.611241338.76Late Middle Age3000238
Anterne0.714711235.29Late Middle Age2001025
Anterne0.826321123.68High Middle Age0000026
Anterne0.923121026.88High Middle Age4200137
Anterne1.08740862.60Early Middle Age1000085
Anterne1.23690713.10Early Middle Age0000001
Anterne1.38660563.40Early Middle Age0000113
Anterne1.54320406.80End Roman period-Early Middle Age0100038
Anterne1.65260297.40End Roman period-Early Middle Age0001006
Anterne1.78840161.60Roman Period0100227
Anterne1.8925057.50Roman Period2200207
Anterne2.00080-50.80Iron Age2403088
Anterne2.12610-176.10Iron Age2220158
Anterne2.23150-281.50Iron Age2000088
Anterne2.33780-387.80Iron Age4400187
Anterne2.44490-494.90Mid Late Bronze Age0001004
Anterne2.54010-590.10Mid Late Bronze Age0000208
Anterne2.62620-676.20Mid Late Bronze Age0001003
Anterne2.77860-828.60Mid Late Bronze Age0003085
Anterne2.87950-929.50Mid Late Bronze Age0001003
Anterne2.99650-1046.50Mid Late Bronze Age0003016
Anterne3.07870-1128.70Mid Late Bronze Age0100006
Anterne3.22970-1279.70Mid Late Bronze Age0000005
Anterne3.32740-1377.40Mid Late Bronze Age0101115
Anterne3.40250-1452.50Early Bronze Age0002012
Anterne3.54350-1593.50Early Bronze Age0000003
Anterne3.71730-1767.30Early Bronze Age0000001
Anterne3.89530-1945.30Early Bronze Age0002012
Anterne4.08410-2134.10Early Bronze Age0003003
Anterne4.34370-2393.70Late Neolithic0001002
Anterne4.58880-2638.80Late Neolithic0000101
Anterne4.85630-2906.30Late Neolithic2001001
Anterne5.02070-3070.70Late Neolithic0003001
Anterne5.15740-3207.40Pre anthropic0002101
Anterne5.29950-3349.50Pre anthropic0004001
Anterne5.55260-3602.60Pre anthropic0006001
Anterne5.97030-4020.30Pre anthropic0007002
Anterne6.38490-4434.90Pre anthropic0001000