Wittenborn, Anna; Bauersachs, Thorsten; Hassenrück, Christiane; Käding, Katja; Jürgens, Klaus (2023): Glycerol dialkyl glycerol tetraethers (GDGT) lipids and CARD-FISH data from suspended particulate matter from the central Baltic Sea [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.958465
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Published: 2023-05-17 • DOI registered: 2023-06-15
Abstract:
During expedition EMB201 in the Baltic Sea we investigated the local producers of glycerol dialkyl glycerol tetraethers (GDGT) and their thriving depth by a combined 16S rRNA gene amplicon sequencing/ CARD-FISH and lipidomic approach. Water samples were taken in December 2018 by a pump-CTD, a giant water sampler and with Niskin bottles at the surface, suboxic and sulfidic zones of the Landsort Deep, Fårö Basin and East Gotland Basin. This data set contains the CARD-FISH and lipidomics data, while the 16S rRNA gene sequencing data is available on ENA. Cell abundance was analysed in an aliquot of 40 ml filtered (pore size 0.22 micro m) sea water fixed with particle-free formaldehyde. Archaeal cells on the filters were specifically hybridized via catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH) using the Cren537 probe. Cells on the hybridized filter were counter-stained with 40,6-diamidin-2-phenylindol (DAPI). For lipid analysis, 150–600 L sea water were filtered with a flow rate of 1.5 L min-1 on pre-ashed, 142-mm-diameter, 0.7µm pore size glass fibre GF/F filters, and frozen at –20 °C. The filters were lyophilized before different extraction methods were used to obtain intact and core GDGTs by ultra-sonification in different solvent mixtures. The combined supernatants were phase separated before analysis by high performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS2) for intact polar lipids and by high performance liquid chromatography coupled to atmospheric pressure chemical ionization mass spectrometry (HPLC APCI-MS; ThermoScientific) for core GDGTs.
Keyword(s):
Supplement to:
Wittenborn, Anna; Bauersachs, Thorsten; Hassenrück, Christiane; Käding, Katja; Jürgens, Klaus (2023): Nitrosopumilus as main source of isoprenoid glycerol dialkyl glycerol tetraether lipids in the central Baltic Sea. Frontiers in Microbiology, 14, https://doi.org/10.3389/fmicb.2023.1216130
Related to:
Wittenborn, Anna; Wäge-Recchioni, Janine; Hassenrück, Christiane; Jürgens, Klaus (2023): 16S rRNA gene amplicon sequences from suspended particulate matter from the central Baltic Sea [dataset]. European Nucleotide Archive (ENA), insdc:PRJEB61728
Funding:
Deutsche Forschungsgemeinschaft, Bonn (DFG), grant/award no. 328543621: A comprehensive study for the application of proxies based on glycerol dialkyl glycerol tetraethers in the Baltic Sea (TETRABAL)
Leibniz Association (Leibniz Association), grant/award no. SAW-2017-IOW-2 649: BaltRap: The Baltic Sea and its southern Lowlands: proxy – environment interactions in time of rapid change
Coverage:
Median Latitude: 58.022807 * Median Longitude: 19.448808 * South-bound Latitude: 57.319070 * West-bound Longitude: 18.266660 * North-bound Latitude: 58.639440 * East-bound Longitude: 20.080790
Date/Time Start: 2018-12-02T22:01:00 * Date/Time End: 2018-12-10T07:43:00
Minimum DEPTH, water: -425 m * Maximum DEPTH, water: -4 m
Event(s):
EMB201_5-3 (Stat. TF 271 Stat. 4) * Latitude Start: 57.319704 * Longitude Start: 20.048070 * Latitude End: 57.320194 * Longitude End: 20.049254 * Date/Time Start: 2018-12-02T22:01:00 * Date/Time End: 2018-12-03T06:48:00 * Elevation Start: -245.5 m * Elevation End: -245.4 m * Location: Baltic Sea * Campaign: EMB201 (BaltRap) * Basis: Elisabeth Mann Borgese * Method/Device: Go-Flo bottles (GOFLO)
EMB201_7-0 * Latitude: 57.319070 * Longitude: 20.050460 * Date/Time: 2018-12-05T20:24:00 * Elevation: -4.0 m * Location: Eastern Gotland Basin * Campaign: EMB201 (BaltRap) * Basis: Elisabeth Mann Borgese * Method/Device: Ships non-toxic pump (GPUMP)
EMB201_7-1 (Station 04 (TF 271)) * Latitude Start: 57.319071 * Longitude Start: 20.050457 * Latitude End: 57.319653 * Longitude End: 20.048042 * Date/Time Start: 2018-12-05T20:23:00 * Date/Time End: 2018-12-05T22:29:00 * Elevation Start: -246.0 m * Elevation End: -245.8 m * Location: Baltic Sea * Campaign: EMB201 (BaltRap) * Basis: Elisabeth Mann Borgese * Method/Device: Go-Flo bottles (GOFLO)
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | ||||
| 2 | Other event | Other event | ||||
| 3 | Station label | Station | ||||
| 4 | DATE/TIME | Date/Time | Geocode | |||
| 5 | LATITUDE | Latitude | Geocode | |||
| 6 | LONGITUDE | Longitude | Geocode | |||
| 7 | Location | Location | ||||
| 8 | Site | Site | ||||
| 9 | DEPTH, water | Depth water | m | Geocode | ||
| 10 | Sample code/label | Sample label | Sample names used for sequence data submission | |||
| 11 | Temperature, water | Temp | °C | Conductivity-Temperature-Depth- profiler (CTD), Sea-Bird Scientific, SBE911 plus | ||
| 12 | Oxygen, dissolved | DO | ml/l | Conductivity-Temperature-Depth- profiler (CTD), Sea-Bird Scientific, SBE911 plus | ||
| 13 | Cells, 4',6-Diamidin-2-phenylindol stained | Cells DAPI | #/ml | Käding, Katja | Catalyzed reporter deposition fluorescence in situ hybridisation (CARD-FISH) | |
| 14 | Crenarchaeota, targeted with Cren537 oligonucleotide FISH-probe | Cren537 | #/ml | Käding, Katja | Catalyzed reporter deposition fluorescence in situ hybridisation (CARD-FISH) | |
| 15 | Monohexose-archaeol | MH AR | peak area/l | Bauersachs, Thorsten | High performance liquid chromatography (HPLC), Waters Corporation, Alliance 2690; coupled with Triple quadrupole tandem mass spectrometer (LC-MS/MS), Micromass, Quattro LC (LC-MS/MS) | |
| 16 | Dihexose-archaeol | DH AR | peak area/l | Bauersachs, Thorsten | High performance liquid chromatography (HPLC), Waters Corporation, Alliance 2690; coupled with Triple quadrupole tandem mass spectrometer (LC-MS/MS), Micromass, Quattro LC (LC-MS/MS) | |
| 17 | Monohexose-macroarchaeol | MH MacroAR | peak area/l | Bauersachs, Thorsten | High performance liquid chromatography (HPLC), Waters Corporation, Alliance 2690; coupled with Triple quadrupole tandem mass spectrometer (LC-MS/MS), Micromass, Quattro LC (LC-MS/MS) | |
| 18 | Monohexose acyclic glycerol dialkyl glycerol tetraether | MH-GDGT-0 | peak area/l | Bauersachs, Thorsten | High performance liquid chromatography (HPLC), Waters Corporation, Alliance 2690; coupled with Triple quadrupole tandem mass spectrometer (LC-MS/MS), Micromass, Quattro LC (LC-MS/MS) | with the headgroup Monohexose |
| 19 | Monohexose monocyclic glycerol dialkyl glycerol tetraether | MH-GDGT-1 | peak area/l | Bauersachs, Thorsten | High performance liquid chromatography (HPLC), Waters Corporation, Alliance 2690; coupled with Triple quadrupole tandem mass spectrometer (LC-MS/MS), Micromass, Quattro LC (LC-MS/MS) | with the headgroup Monohexose |
| 20 | Monohexose dicyclic glycerol dialkyl glycerol tetraether | MH-GDGT-2 | peak area/l | Bauersachs, Thorsten | High performance liquid chromatography (HPLC), Waters Corporation, Alliance 2690; coupled with Triple quadrupole tandem mass spectrometer (LC-MS/MS), Micromass, Quattro LC (LC-MS/MS) | with the headgroup Monohexose |
| 21 | Monohexose tricyclic glycerol dialkyl glycerol tetraether | MH-GDGT-3 | peak area/l | Bauersachs, Thorsten | High performance liquid chromatography (HPLC), Waters Corporation, Alliance 2690; coupled with Triple quadrupole tandem mass spectrometer (LC-MS/MS), Micromass, Quattro LC (LC-MS/MS) | with the headgroup Monohexose |
| 22 | Monohexose crenarchaeol | MH-GDGT-cren | peak area/l | Bauersachs, Thorsten | High performance liquid chromatography (HPLC), Waters Corporation, Alliance 2690; coupled with Triple quadrupole tandem mass spectrometer (LC-MS/MS), Micromass, Quattro LC (LC-MS/MS) | with the headgroup Monohexose |
| 23 | Monohexose crenarchaeol regio-isomer | MH-GDGT-cren' | peak area/l | Bauersachs, Thorsten | High performance liquid chromatography (HPLC), Waters Corporation, Alliance 2690; coupled with Triple quadrupole tandem mass spectrometer (LC-MS/MS), Micromass, Quattro LC (LC-MS/MS) | with the headgroup Monohexose |
| 24 | Dihexose acyclic glycerol dialkyl glycerol tetraether | DH-GDGT-0 | peak area/l | Bauersachs, Thorsten | High performance liquid chromatography (HPLC), Waters Corporation, Alliance 2690; coupled with Triple quadrupole tandem mass spectrometer (LC-MS/MS), Micromass, Quattro LC (LC-MS/MS) | with the headgroup Dihexose |
| 25 | Dihexose monocyclic glycerol dialkyl glycerol tetraether | DH-GDGT-1 | peak area/l | Bauersachs, Thorsten | High performance liquid chromatography (HPLC), Waters Corporation, Alliance 2690; coupled with Triple quadrupole tandem mass spectrometer (LC-MS/MS), Micromass, Quattro LC (LC-MS/MS) | with the headgroup Dihexose |
| 26 | Dihexose dicyclic glycerol dialkyl glycerol tetraether | DH-GDGT-2 | peak area/l | Bauersachs, Thorsten | High performance liquid chromatography (HPLC), Waters Corporation, Alliance 2690; coupled with Triple quadrupole tandem mass spectrometer (LC-MS/MS), Micromass, Quattro LC (LC-MS/MS) | with the headgroup Dihexose |
| 27 | Dihexose tricyclic glycerol dialkyl glycerol tetraether | DH-GDGT-3 | peak area/l | Bauersachs, Thorsten | High performance liquid chromatography (HPLC), Waters Corporation, Alliance 2690; coupled with Triple quadrupole tandem mass spectrometer (LC-MS/MS), Micromass, Quattro LC (LC-MS/MS) | with the headgroup Dihexose |
| 28 | Dihexose crenarchaeol | DH-GDGT-cren | peak area/l | Bauersachs, Thorsten | High performance liquid chromatography (HPLC), Waters Corporation, Alliance 2690; coupled with Triple quadrupole tandem mass spectrometer (LC-MS/MS), Micromass, Quattro LC (LC-MS/MS) | with the headgroup Dihexose |
| 29 | Phosphohexose acyclic glycerol dialkyl glycerol tetraether | PH-GDGT-0 | peak area/l | Bauersachs, Thorsten | High performance liquid chromatography (HPLC), Waters Corporation, Alliance 2690; coupled with Triple quadrupole tandem mass spectrometer (LC-MS/MS), Micromass, Quattro LC (LC-MS/MS) | with the headgroup Phosphohexose |
| 30 | Phosphohexose monocyclic glycerol dialkyl glycerol tetraether | PH-GDGT-1 | peak area/l | Bauersachs, Thorsten | High performance liquid chromatography (HPLC), Waters Corporation, Alliance 2690; coupled with Triple quadrupole tandem mass spectrometer (LC-MS/MS), Micromass, Quattro LC (LC-MS/MS) | with the headgroup Phosphohexose |
| 31 | Phosphohexose dicyclic glycerol dialkyl glycerol tetraether | PH-GDGT-2 | peak area/l | Bauersachs, Thorsten | High performance liquid chromatography (HPLC), Waters Corporation, Alliance 2690; coupled with Triple quadrupole tandem mass spectrometer (LC-MS/MS), Micromass, Quattro LC (LC-MS/MS) | with the headgroup Phosphohexose |
| 32 | Phosphohexose tricyclic glycerol dialkyl glycerol tetraether | PH-GDGT-3 | peak area/l | Bauersachs, Thorsten | High performance liquid chromatography (HPLC), Waters Corporation, Alliance 2690; coupled with Triple quadrupole tandem mass spectrometer (LC-MS/MS), Micromass, Quattro LC (LC-MS/MS) | with the headgroup Phosphohexose |
| 33 | Phosphohexose crenarchaeol | PH-GDGT-cren | peak area/l | Bauersachs, Thorsten | High performance liquid chromatography (HPLC), Waters Corporation, Alliance 2690; coupled with Triple quadrupole tandem mass spectrometer (LC-MS/MS), Micromass, Quattro LC (LC-MS/MS) | with the headgroup Phosphohexose |
| 34 | Core acyclic glycerol dialkyl glycerol tetraether | C-GDGT-0 | ng/l | Wittenborn, Anna | Ultra high performance liquid chromatography (UHPLC), Dionex Corporation, UltiMate 3000 RS; coupled with Single quadrupole mass spectrometer, Thermo Scientific, MSQ Plus | |
| 35 | Core monocyclic glycerol dialkyl glycerol tetraether | C-GDGT-1 | ng/l | Wittenborn, Anna | Ultra high performance liquid chromatography (UHPLC), Dionex Corporation, UltiMate 3000 RS; coupled with Single quadrupole mass spectrometer, Thermo Scientific, MSQ Plus | |
| 36 | Core dicyclic glycerol dialkyl glycerol tetraether | C-GDGT-2 | ng/l | Wittenborn, Anna | Ultra high performance liquid chromatography (UHPLC), Dionex Corporation, UltiMate 3000 RS; coupled with Single quadrupole mass spectrometer, Thermo Scientific, MSQ Plus | |
| 37 | Core tricyclic glycerol dialkyl glycerol tetraether | C-GDGT-3 | ng/l | Wittenborn, Anna | Ultra high performance liquid chromatography (UHPLC), Dionex Corporation, UltiMate 3000 RS; coupled with Single quadrupole mass spectrometer, Thermo Scientific, MSQ Plus | |
| 38 | Core crenarchaeol | C-GDGT-cren | ng/l | Wittenborn, Anna | Ultra high performance liquid chromatography (UHPLC), Dionex Corporation, UltiMate 3000 RS; coupled with Single quadrupole mass spectrometer, Thermo Scientific, MSQ Plus | |
| 39 | Core crenarchaeol regio-isomer | C-GDGT-cren' | ng/l | Wittenborn, Anna | Ultra high performance liquid chromatography (UHPLC), Dionex Corporation, UltiMate 3000 RS; coupled with Single quadrupole mass spectrometer, Thermo Scientific, MSQ Plus |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
371 data points