Coffey, William D; Nardone, Jessica A; Yarram, Aparna; Long, W Christopher; Swiney, Katherine M; Foy, Robert J; Dickinson, Gary H (2023): Seawater carbonate chemistry and micromechanical properties of the mineralized cuticle in juvenile red and blue king crabs [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.958450
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Abstract:
Ocean acidification (OA) adversely affects a broad range of marine calcifying organisms. Crustaceans, however, exhibit mixed responses to OA, with growth or survival negatively affected in some species, but unaffected or positively affected in others. In crustaceans, the mineralized cuticle resists mechanical loads, provides protection from the environment, and enables mobility, but little is known about how OA or interactions between OA and temperature affect its structure or function. Here, the effects of OA on the mechanics, structure, and composition of the cuticle in two Alaska king crab species was assessed. Juvenile blue king crabs (Paralithodes platypus) were exposed for a year to three pH levels, 8.1 (ambient), 7.8 and 7.5. Juvenile red king crabs (Paralithodes camtschaticus) were exposed for ~ 6 months to two pH levels, 8.0 and 7.8, at three temperatures: ambient, ambient + 2 °C, and ambient + 4 °C. Cuticle microhardness (a measure of resistance to permanent or plastic mechanical deformation), thickness, ultrastructure, and elemental composition were assessed in two body regions, the carapace and the crushing chela (claw). In both species tested, OA reduced endocuticle microhardness in the chela, but not in the carapace. There was no effect of pH or temperature on total procuticle thickness of the chela or carapace in either species. Reductions in microhardness were not driven by reduced calcium content of the shell. In fact, calcium content was significantly elevated in the carapace of blue king crabs and in the chela of red king crabs exposed to lower than ambient pH at ambient temperature, suggesting that calcium content alone is not a sufficient proxy for mechanical properties. Reduced chela microhardness, indicative of more compliant material, could compromise the utility of crushing chelae in feeding and defense.
Keyword(s):
Animalia; Arthropoda; Benthic animals; Biomass/Abundance/Elemental composition; Containers and aquaria (20-1000 L or < 1 m**2); Growth/Morphology; Laboratory experiment; Laboratory strains; Not applicable; Other studied parameter or process; Paralithodes camtschaticus; Paralithodes platypus; Single species; Temperature
Supplement to:
Coffey, William D; Nardone, Jessica A; Yarram, Aparna; Long, W Christopher; Swiney, Katherine M; Foy, Robert J; Dickinson, Gary H (2017): Ocean acidification leads to altered micromechanical properties of the mineralized cuticle in juvenile red and blue king crabs. Journal of Experimental Marine Biology and Ecology, 495, 1-12, https://doi.org/10.1016/j.jembe.2017.05.011
Documentation:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2022): seacarb: seawater carbonate chemistry with R. R package version 3.3.1. https://cran.r-project.org/web/packages/seacarb/index.html
Project(s):
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-05-04.
Parameter(s):
# | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
---|---|---|---|---|---|---|
1 | Type of study | Study type | Coffey, William D | |||
2 | Species, unique identification | Species UID | Coffey, William D | |||
3 | Species, unique identification (URI) | Species UID (URI) | Coffey, William D | |||
4 | Species, unique identification (Semantic URI) | Species UID (Semantic URI) | Coffey, William D | |||
5 | Sample number | Sample no | Coffey, William D | |||
6 | Treatment | Treat | Coffey, William D | pH | ||
7 | Treatment | Treat | Coffey, William D | Temperature | ||
8 | Location | Location | Coffey, William D | |||
9 | Microhardness | Microhardness | Coffey, William D | Vickers hardness numbers | ||
10 | Thickness | Thick | µm | Coffey, William D | Exocuticle | |
11 | Thickness | Thick | µm | Coffey, William D | Endocuticle | |
12 | Thickness | Thick | µm | Coffey, William D | Total Procuticle | |
13 | Thickness | Thick | µm | Coffey, William D | Bouligand | |
14 | Carbon | C | % | Coffey, William D | ||
15 | Oxygen | O | % | Coffey, William D | ||
16 | Sodium | Na | % | Coffey, William D | ||
17 | Magnesium | Mg | % | Coffey, William D | ||
18 | Silicon | Si | % | Coffey, William D | ||
19 | Strontium | Sr | % | Coffey, William D | ||
20 | Phosphorus | P | % | Coffey, William D | ||
21 | Sulfur | S | % | Coffey, William D | ||
22 | Chlorine | Cl | % | Coffey, William D | ||
23 | Calcium | Ca | % | Coffey, William D | ||
24 | pH | pH | Coffey, William D | Potentiometric | free scale | |
25 | pH, standard deviation | pH std dev | ± | Coffey, William D | Potentiometric | free scale |
26 | Temperature, water | Temp | °C | Coffey, William D | ||
27 | Temperature, water, standard deviation | Temp std dev | ± | Coffey, William D | ||
28 | Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) | pCO2water_SST_wet | µatm | Coffey, William D | ||
29 | Partial pressure of carbon dioxide, standard deviation | pCO2 std dev | ± | Coffey, William D | ||
30 | Bicarbonate ion | [HCO3]- | µmol/kg | Coffey, William D | ||
31 | Bicarbonate ion, standard deviation | [HCO3]- std dev | ± | Coffey, William D | ||
32 | Carbonate ion | [CO3]2- | µmol/kg | Coffey, William D | ||
33 | Carbonate ion, standard deviation | [CO3]2- std dev | ± | Coffey, William D | ||
34 | Carbon, inorganic, dissolved | DIC | µmol/kg | Coffey, William D | Coulometric titration | |
35 | Carbon, inorganic, dissolved, standard deviation | DIC std dev | ± | Coffey, William D | Coulometric titration | |
36 | Alkalinity, total | AT | µmol/kg | Coffey, William D | Potentiometric titration | |
37 | Alkalinity, total, standard deviation | AT std dev | ± | Coffey, William D | Potentiometric titration | |
38 | Aragonite saturation state | Omega Arg | Coffey, William D | |||
39 | Aragonite saturation state, standard deviation | Omega Arg std dev | ± | Coffey, William D | ||
40 | Calcite saturation state | Omega Cal | Coffey, William D | |||
41 | Calcite saturation state, standard deviation | Omega Cal std dev | ± | Coffey, William D | ||
42 | Salinity | Sal | Coffey, William D | |||
43 | Salinity, standard deviation | Sal std dev | ± | Coffey, William D | ||
44 | Carbonate system computation flag | CSC flag | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | ||
45 | pH | pH | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | total scale | |
46 | Carbon dioxide | CO2 | µmol/kg | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | |
47 | Fugacity of carbon dioxide (water) at sea surface temperature (wet air) | fCO2water_SST_wet | µatm | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | |
48 | Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) | pCO2water_SST_wet | µatm | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | |
49 | Bicarbonate ion | [HCO3]- | µmol/kg | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | |
50 | Carbonate ion | [CO3]2- | µmol/kg | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | |
51 | Aragonite saturation state | Omega Arg | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) | ||
52 | Calcite saturation state | Omega Cal | Yang, Yan | Calculated using seacarb after Nisumaa et al. (2010) |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
5334 data points
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