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Spilling, Kristian; Piiparinen, Jonna; Achterberg, Eric Pieter; Arístegui, Javier; Bach, Lennart Thomas; Camarena-Gómez, Maria-Teresa; von der Esch, Elisabeth; Fischer, Martin A; Gómez-Letona, Markel; Hernández-Hernández, Nauzet; Meyer, Judith; Schmitz, Ruth A; Riebesell, Ulf (2023): Enzyme activity during a mesocosm experiment in the Peruvian upwelling system: bacteria [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.958425, In: Spilling, K et al. (2023): Enzyme activity during a mesocosm experiment in the Peruvian upwelling system [dataset publication series]. PANGAEA, https://doi.org/10.1594/PANGAEA.958426

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Abstract:
The data is from a mesocosm experiment set up outside Lima, Peru to study the influence of upwelling of oxygen minimum zone (OMZ) water.
The mesocosm bags were 2 m in diameter and extended from the surface down to 19 m depth, where the last 2 m was a conical sediment trap. Eight mesocosm bags were used and they were moored at 12.0555°S; 77.2348°W just north of Isla San Lorenzo where the water depth is ~30 m. The experiment was started 25 February 2017 by closing the mesocosm bags and were run for 50 days.
Two treatments were used (water with different OMZ signature), each with four replicates. Water (100 m3) from the OMZ was collected from two locations and depths. The first was collected from 12.028323°S; 77.223603°W from 30 m depth, and the second one from 12.044333°S; 77.377583°W from 70 m depth. The original aim was to collect severe and moderate OMZ signature water (differing in e.g. nitrate concentrations) from the first and second site, respectively. This assumption was based on long-term monitoring data, however, the chemical properties (e.g. nitrate concentration) was more similar in these water masses than anticipated, rather reflecting low and very low OMZ signatures from site 1 and 2 respectively.
To have a baseline of measured variables, the mesocosms where closed and environmental and biological variables were determined over 10 days. After this period, the OMZ water was added to the mesocosms in two steps on day 11 and 12 after the enclosure of the mesocosms. As the mesocosms contain a specific volume (~54 m3), the process of adding the OMZ water started with first removing water from the mesocosms. The water removed (~20 m3) was pumped out from 11-12 m depth. A similar volume of OMZ water, from both collection sites, was then pumped into four replicate mesocosms each. The OMZ water was pumped into the mesocosms moving the input hose between 14-17 m depth. The water collected at 30 m depth was pumped into mesocosms M1, M4, M5 and M8 having a low OMZ signature and water from 70 m depth into mesocosms M2, M3, M6 and M7 having a very low OMZ signature. Due a halocline at 12 m depth (see below), the added OMZ water was not immediately mixed throughout the mesocosm bag.
Sampling took place every second day over a period of 50 days, and all variables were taken with an integrated water sampler (HydroBios, IWS) pre-programed to fill from 0 – 10 m depth and all samples consisted of this integrated samples from the upper 10 m. The samples were stored dark in cool boxes and brought back to the laboratory and processed right away. Sampling took place in the morning, and the samples were usually back in the laboratory around noon.
Measured variables included inorganic nutrients, dissolved organic nutrients, extracellular enzyme activity: leucine aminopeptidase (LAP) and alkaline phosphatase activity (APA), and the phytoplankton and bacterial community composition.
Keyword(s):
alkaline phosphatase activity; Bacteria; chlorophyll-a; leucine aminopeptidase; oxygen minimum zone; Phytoplankton
Project(s):
Climate - Biogeochemistry Interactions in the Tropical Ocean (SFB754)
Network of Leading European AQUAtic MesoCOSM Facilities Connecting Mountains to Oceans from the Arctic to the Mediterranean (AQUACOSM)
Funding:
Academy of Finland, grant/award no. 259164
German Research Foundation (DFG), grant/award no. 27542298: Climate - Biogeochemistry Interactions in the Tropical Ocean
Horizon 2020 (H2020), grant/award no. 731065: Network of Leading European AQUAtic MesoCOSM Facilities Connecting Mountains to Oceans from the Arctic to the Mediterranean
Coverage:
Latitude: -12.055000 * Longitude: -77.235000
Date/Time Start: 2017-03-07T00:00:00 * Date/Time End: 2017-04-08T00:00:00
Event(s):
KOSMOS_2017_Peru * Latitude: -12.055000 * Longitude: -77.235000 * Date/Time Start: 2017-02-23T00:00:00 * Date/Time End: 2017-04-10T00:00:00 * Campaign: KOSMOS_2017 (KOSMOS Peru) * Method/Device: Mesocosm experiment (MESO)
Comment:
Bacteria are given in percent of total operational taxonomic unit.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1DATE/TIMEDate/TimeSpilling, KristianGeocode
2Day of experimentDOEdaySpilling, Kristian
3Mesocosm labelMeso labelRiebesell, Ulf
4Experimental treatmentExp treatRiebesell, Ulf
5Leucine aminopeptidase activityLeu aminopepµmol/l/hPiiparinen, JonnaLAP
6Alkaline phosphatase activityALKP Anmol/l/hMeyer, Judithrecalculated from nmol/l/day
7BacteriaBactSchmitz, Ruth AActinomarinales
8BacteriaBactSchmitz, Ruth AMicrotrichales
9BacteriaBactSchmitz, Ruth AActinobacteria_unclassified
10BacteriaBactSchmitz, Ruth ACorynebacteriales
11BacteriaBactSchmitz, Ruth AMicrococcales
12BacteriaBactSchmitz, Ruth APeM15
13BacteriaBactSchmitz, Ruth APropionibacteriales
14BacteriaBactSchmitz, Ruth ABacteria_unclassified
15BacteriaBactSchmitz, Ruth ABacteroidales
16BacteriaBactSchmitz, Ruth ABacteroidetes_VC2.1_Bac22
17BacteriaBactSchmitz, Ruth ABacteroidia_unclassified
18BacteriaBactSchmitz, Ruth AChitinophagales
19BacteriaBactSchmitz, Ruth ACytophagales
20BacteriaBactSchmitz, Ruth AFlavobacteriales
21BacteriaBactSchmitz, Ruth ASphingobacteriales
22BacteriaBactSchmitz, Ruth ABalneolales
23BacteriaBactSchmitz, Ruth ARhodothermales
24BacteriaBactSchmitz, Ruth ACalditrichales
25BacteriaBactSchmitz, Ruth AAnaerolineales
26BacteriaBactSchmitz, Ruth ASAR202_clade
27BacteriaBactSchmitz, Ruth ACloacimonadales
28BacteriaBactSchmitz, Ruth AOxyphotobacteria_unclassified
29BacteriaBactSchmitz, Ruth ASynechococcales
30BacteriaBactSchmitz, Ruth ADadabacteriales
31BacteriaBactSchmitz, Ruth ADeinococcales
32BacteriaBactSchmitz, Ruth ABabeliales
33BacteriaBactSchmitz, Ruth ACampylobacterales
34BacteriaBactSchmitz, Ruth AChitinivibrionales
35BacteriaBactSchmitz, Ruth AFibrobacteres_unclassified
36BacteriaBactSchmitz, Ruth ABacillales
37BacteriaBactSchmitz, Ruth ALactobacillales
38BacteriaBactSchmitz, Ruth AClostridiales
39BacteriaBactSchmitz, Ruth AErysipelotrichales
40BacteriaBactSchmitz, Ruth AFusobacteriales
41BacteriaBactSchmitz, Ruth AHydrogenedentiales
42BacteriaBactSchmitz, Ruth AKiritimatiellales
43BacteriaBactSchmitz, Ruth ALCP-89_or
44BacteriaBactSchmitz, Ruth ALatescibacteria_or
45BacteriaBactSchmitz, Ruth ALentisphaerae_unclassified
46BacteriaBactSchmitz, Ruth ALentisphaerales
47BacteriaBactSchmitz, Ruth AMargulisbacteria_or
48BacteriaBactSchmitz, Ruth AMarinimicrobia_(SAR406_clade)_or
49BacteriaBactSchmitz, Ruth ANitrospinales
50BacteriaBactSchmitz, Ruth AOmnitrophicaeota_or
51BacteriaBactSchmitz, Ruth AABY1_unclassified
52BacteriaBactSchmitz, Ruth ACandidatus_Magasanikbacteria
53BacteriaBactSchmitz, Ruth ACandidatus_Uhrbacteria
54BacteriaBactSchmitz, Ruth ACandidatus_Peregrinibacteria
55BacteriaBactSchmitz, Ruth AGracilibacteria_or
56BacteriaBactSchmitz, Ruth AGracilibacteria_unclassified
57BacteriaBactSchmitz, Ruth AJGI_0000069-P22
58BacteriaBactSchmitz, Ruth ACandidatus_Pacebacteria
59BacteriaBactSchmitz, Ruth ACandidatus_Campbellbacteria
60BacteriaBactSchmitz, Ruth ACandidatus_Kaiserbacteria
61BacteriaBactSchmitz, Ruth AParcubacteria_unclassified
62BacteriaBactSchmitz, Ruth ASaccharimonadales
63BacteriaBactSchmitz, Ruth AOM190_or
64BacteriaBactSchmitz, Ruth APhycisphaerales
65BacteriaBactSchmitz, Ruth AAlphaproteobacteria_unclassified
66BacteriaBactSchmitz, Ruth ACaulobacterales
67BacteriaBactSchmitz, Ruth AHolosporales
68BacteriaBactSchmitz, Ruth AKordiimonadales
69BacteriaBactSchmitz, Ruth AMicavibrionales
70BacteriaBactSchmitz, Ruth AParvibaculales
71BacteriaBactSchmitz, Ruth APuniceispirillales
72BacteriaBactSchmitz, Ruth ARhizobiales
73BacteriaBactSchmitz, Ruth ARhodobacterales
74BacteriaBactSchmitz, Ruth ARhodospirillales
75BacteriaBactSchmitz, Ruth ARickettsiales
76BacteriaBactSchmitz, Ruth ASAR11_clade
77BacteriaBactSchmitz, Ruth ASneathiellales
78BacteriaBactSchmitz, Ruth ASphingomonadales
79BacteriaBactSchmitz, Ruth AThalassobaculales
80BacteriaBactSchmitz, Ruth Auncultured
81BacteriaBactSchmitz, Ruth ABdellovibrionales
82BacteriaBactSchmitz, Ruth ABradymonadales
83BacteriaBactSchmitz, Ruth ADeltaproteobacteria_unclassified
84BacteriaBactSchmitz, Ruth ADesulfobacterales
85BacteriaBactSchmitz, Ruth ADesulfovibrionales
86BacteriaBactSchmitz, Ruth AMyxococcales
87BacteriaBactSchmitz, Ruth AOligoflexales
88BacteriaBactSchmitz, Ruth APB19
89BacteriaBactSchmitz, Ruth ASAR324_clade(Marine_group_B)
90BacteriaBactSchmitz, Ruth AAlteromonadales
91BacteriaBactSchmitz, Ruth AArenicellales
92BacteriaBactSchmitz, Ruth ABetaproteobacteriales
93BacteriaBactSchmitz, Ruth ACH2b56
94BacteriaBactSchmitz, Ruth ACellvibrionales
95BacteriaBactSchmitz, Ruth AChromatiales
96BacteriaBactSchmitz, Ruth ACoxiellales
97BacteriaBactSchmitz, Ruth AEctothiorhodospirales
98BacteriaBactSchmitz, Ruth AEnterobacteriales
99BacteriaBactSchmitz, Ruth AFrancisellales
100BacteriaBactSchmitz, Ruth AGammaproteobacteria_Incertae_Sedis
101BacteriaBactSchmitz, Ruth AGammaproteobacteria_unclassified
102BacteriaBactSchmitz, Ruth AHOC36
103BacteriaBactSchmitz, Ruth AKI89A_clade
104BacteriaBactSchmitz, Ruth ALegionellales
105BacteriaBactSchmitz, Ruth AMBAE14
106BacteriaBactSchmitz, Ruth AMethylococcales
107BacteriaBactSchmitz, Ruth ANitrosococcales
108BacteriaBactSchmitz, Ruth AOM182_clade
109BacteriaBactSchmitz, Ruth AOceanospirillales
110BacteriaBactSchmitz, Ruth APiscirickettsiales
111BacteriaBactSchmitz, Ruth APseudomonadales
112BacteriaBactSchmitz, Ruth ASAR86_clade
113BacteriaBactSchmitz, Ruth ASalinisphaerales
114BacteriaBactSchmitz, Ruth ASteroidobacterales
115BacteriaBactSchmitz, Ruth ATenderiales
116BacteriaBactSchmitz, Ruth AThiomicrospirales
117BacteriaBactSchmitz, Ruth AThiotrichales
118BacteriaBactSchmitz, Ruth AUBA10353_marine_group
119BacteriaBactSchmitz, Ruth AVibrionales
120BacteriaBactSchmitz, Ruth AXanthomonadales
121BacteriaBactSchmitz, Ruth AProteobacteria_unclassified
122BacteriaBactSchmitz, Ruth ASpirochaetales
123BacteriaBactSchmitz, Ruth AEntomoplasmatales
124BacteriaBactSchmitz, Ruth AIzimaplasmatales
125BacteriaBactSchmitz, Ruth AOpitutales
126BacteriaBactSchmitz, Ruth APedosphaerales
127BacteriaBactSchmitz, Ruth AVerrucomicrobiales
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
5040 data points

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