Citation:

PANGAEA.958009

Name: Seawater carbonate chemistry and larval growth rates of sea urchin Strongylocentrotus droebachiensis
Published: 2023-04-26
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Echinodermata; Gene expression (incl. proteomics); Growth/Morphology; Laboratory experiment; Mortality/Survival; North Atlantic; Pelagos; Polar; Single species; Strongylocentrotus droebachiensis; Temperate; Zooplankton

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Abstract:

Ocean acidification (OA) is increasing due to anthropogenic CO2 emissions, and poses a threat to marine species and communities worldwide. To better project the effects of acidification on organisms' health and persistence an understanding is needed of (1) the mechanisms underlying developmental and physiological tolerance, and (2) the potential populations have for rapid evolutionary adaptation. This is especially challenging in non-model species where targeted assays of metabolism and stress physiology may not be available or economical for large-scale assessments of genetic constraints. We used mRNA sequencing and a quantitative genetics breeding design to study mechanisms underlying genetic variability and tolerance to decreased seawater pH (-0.4 pH units) in larvae of the sea urchin Strongylocentrotus droebachiensis. We used a gene ontology-based approach to integrate expression profiles into indirect measures of cellular and biochemical traits underlying variation in larval performance (i.e., growth rates). Molecular responses to OA were complex, involving changes to several functions such as growth rates, cell division, metabolism, and immune activities. Surprisingly, the magnitude of pH effects on molecular traits tended to be small relative to variation attributable to segregating functional genetic variation in this species. We discuss how the application of transcriptomics and quantitative genetics approaches across diverse species can enrich our understanding of the biological impacts of climate change.

Keyword(s):

Animalia; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Echinodermata; Gene expression (incl. proteomics); Growth/Morphology; Laboratory experiment; Mortality/Survival; North Atlantic; Pelagos; Polar; Single species; Strongylocentrotus droebachiensis; Temperate; Zooplankton

Supplement to:

Runcie, Daniel E; Dorey, Narimane; Garfield, David; Stumpp, Meike; Dupont, Sam; Wray, Gregory A (2017): Genomic characterization of the evolutionary potential of the sea urchin Strongylocentrotus droebachiensis facing ocean acidification. Genome Biology and Evolution, evw272, https://doi.org/10.1093/gbe/evw272

Source:

Runcie, Daniel E; Dorey, Narimane; Garfield, David; Stumpp, Meike; Dupont, Sam; Wray, Gregory A (2017): Data from: Genomic characterization of the evolutionary potential of the sea urchin Strongylocentrotus droebachiensis facing ocean acidification. Dryad, https://doi.org/10.5061/dryad.1f6t8

Documentation:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2022): seacarb: seawater carbonate chemistry with R. R package version 3.3.1. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Median Latitude: 63.675000 * Median Longitude: 17.583300 * South-bound Latitude: 56.700000 * West-bound Longitude: 11.516600 * North-bound Latitude: 70.650000 * East-bound Longitude: 23.650000

Event(s):

Hammerfest * Latitude: 70.650000 * Longitude: 23.650000 * Method/Device: Experiment (EXP)

northern_Denmark * Latitude: 56.700000 * Longitude: 11.516600 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-04-26.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type of study	Study type		Runcie, Daniel E
2	Species, unique identification	Species UID		Runcie, Daniel E
3	Species, unique identification (URI)	Species UID (URI)		Runcie, Daniel E
4	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		Runcie, Daniel E
5	Identification	ID		Runcie, Daniel E		Culture
6	Identification	ID		Runcie, Daniel E		Mother
7	Identification	ID		Runcie, Daniel E		Father
8	Treatment	Treat		Runcie, Daniel E
9	Site	Site		Runcie, Daniel E		Mother
10	Site	Site		Runcie, Daniel E		Father
11	Growth rate	µ	mm/day	Runcie, Daniel E		Larval
12	Larvae, alive	Larvae alive	#	Runcie, Daniel E		Counted at day 6
13	Salinity	Sal		Runcie, Daniel E
14	pH	pH		Runcie, Daniel E	Potentiometric	total scale
15	pH, standard deviation	pH std dev	±	Runcie, Daniel E	Potentiometric	total scale
16	Temperature, water	Temp	°C	Runcie, Daniel E
17	Temperature, water, standard deviation	Temp std dev	±	Runcie, Daniel E
18	Carbon, inorganic, dissolved	DIC	µmol/kg	Runcie, Daniel E	Colorimetric
19	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Runcie, Daniel E	Colorimetric
20	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Runcie, Daniel E	Calculated using seacarb
21	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Runcie, Daniel E	Calculated using seacarb
22	Aragonite saturation state	Omega Arg		Runcie, Daniel E	Calculated using seacarb
23	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Runcie, Daniel E	Calculated using seacarb
24	Calcite saturation state	Omega Cal		Runcie, Daniel E	Calculated using seacarb
25	Calcite saturation state, standard deviation	Omega Cal std dev	±	Runcie, Daniel E	Calculated using seacarb
26	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
27	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
28	Carbon dioxide, standard deviation	CO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
29	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	Fugacity of carbon dioxide in seawater, standard deviation	fCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
31	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
32	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
33	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
35	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Carbonate ion, standard deviation	[CO3]2- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
37	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Alkalinity, total, standard deviation	AT std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
39	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
40	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
41	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Calcite saturation state, standard deviation	Omega Cal std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1680 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Study type	2 Species UID	3 Species UID (URI)	4 Species UID (Semantic URI)	5 ID (Culture)	6 ID (Mother)	7 ID (Father)	8 Treat	9 Site (Mother)	10 Site (Father)	11 µ [mm/day] (Larval)	12 Larvae alive [#] (Counted at day 6)	13 Sal	14 pH (total scale, Potentiometric)	15 pH std dev [±] (total scale, Potentiometric)	16 Temp [°C]	17 Temp std dev [±]	18 DIC [µmol/kg] (Colorimetric)	19 DIC std dev [±] (Colorimetric)	20 pCO2water_SST_wet [µatm] (Calculated using seacarb)	21 pCO2 std dev [±] (Calculated using seacarb)	22 Omega Arg (Calculated using seacarb)	23 Omega Arg std dev [±] (Calculated using seacarb)	24 Omega Cal (Calculated using seacarb)	25 Omega Cal std dev [±] (Calculated using seacarb)	26 CSC flag (Calculated using seacarb afte...)	27 CO2 [µmol/kg] (Calculated using seacarb afte...)	28 CO2 std dev [±] (Calculated using seacarb afte...)	29 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	30 fCO2 std dev [±] (Calculated using seacarb afte...)	31 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	32 pCO2 std dev [±] (Calculated using seacarb afte...)	33 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	34 [HCO3]- std dev [±] (Calculated using seacarb afte...)	35 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	36 [CO3]2- std dev [±] (Calculated using seacarb afte...)	37 AT [µmol/kg] (Calculated using seacarb afte...)	38 AT std dev [±] (Calculated using seacarb afte...)	39 Omega Arg (Calculated using seacarb afte...)	40 Omega Arg std dev [±] (Calculated using seacarb afte...)	41 Omega Cal (Calculated using seacarb afte...)	42 Omega Cal std dev [±] (Calculated using seacarb afte...)
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	AD_Ctl	A	D	Control	northern Denmark	northern Denmark	0.0380	42	34.7	8.00	0.03	10.17	0.20	2000	80	419	39	1.76	0.14	2.77	0.22	9	18.35	1.52	419.85	34.73	421.47	34.86	1867.43	74.96	114.22	8.83	2156.15	84.76	1.73	0.13	2.73	0.21
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	AD_Low	A	D	Low pH	northern Denmark	northern Denmark	0.0336	26	34.7	7.62	0.11	10.34	0.24	2130	60	1145	296	0.83	0.25	1.30	0.40	9	47.65	12.17	1096.32	279.98	1100.54	281.06	2030.23	57.20	52.11	13.26	2161.93	68.02	0.79	0.20	1.24	0.32
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	YB_Ctl	Y	B	Control	northern Norway	northern Denmark	0.0421	76	34.7	8.00	0.03	10.17	0.20	2000	80	419	39	1.76	0.14	2.77	0.22	9	18.35	1.52	419.85	34.73	421.47	34.86	1867.43	74.96	114.22	8.83	2156.15	84.76	1.73	0.13	2.73	0.21
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	YB_Low	Y	B	Low pH	northern Norway	northern Denmark	0.0384	93	34.7	7.62	0.11	10.34	0.24	2130	60	1145	296	0.83	0.25	1.30	0.40	9	47.65	12.17	1096.32	279.98	1100.54	281.06	2030.23	57.20	52.11	13.26	2161.93	68.02	0.79	0.20	1.24	0.32
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	AB_Ctl	A	B	Control	northern Denmark	northern Denmark	0.0378	39	34.7	8.00	0.03	10.17	0.20	2000	80	419	39	1.76	0.14	2.77	0.22	9	18.35	1.52	419.85	34.73	421.47	34.86	1867.43	74.96	114.22	8.83	2156.15	84.76	1.73	0.13	2.73	0.21
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	AZ_Ctl	A	Z	Control	northern Denmark	northern Norway	0.0388	31	34.7	8.00	0.03	10.17	0.20	2000	80	419	39	1.76	0.14	2.77	0.22	9	18.35	1.52	419.85	34.73	421.47	34.86	1867.43	74.96	114.22	8.83	2156.15	84.76	1.73	0.13	2.73	0.21
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	AX_Ctl	A	X	Control	northern Denmark	northern Norway	0.0419	29	34.7	8.00	0.03	10.17	0.20	2000	80	419	39	1.76	0.14	2.77	0.22	9	18.35	1.52	419.85	34.73	421.47	34.86	1867.43	74.96	114.22	8.83	2156.15	84.76	1.73	0.13	2.73	0.21
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	AW_Ctl	A	W	Control	northern Denmark	northern Norway	0.0401	45	34.7	8.00	0.03	10.17	0.20	2000	80	419	39	1.76	0.14	2.77	0.22	9	18.35	1.52	419.85	34.73	421.47	34.86	1867.43	74.96	114.22	8.83	2156.15	84.76	1.73	0.13	2.73	0.21
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	BB_Ctl	B	B	Control	northern Denmark	northern Denmark	0.0370	74	34.7	8.00	0.03	10.17	0.20	2000	80	419	39	1.76	0.14	2.77	0.22	9	18.35	1.52	419.85	34.73	421.47	34.86	1867.43	74.96	114.22	8.83	2156.15	84.76	1.73	0.13	2.73	0.21
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	BZ_Ctl	B	Z	Control	northern Denmark	northern Norway	0.0400	43	34.7	8.00	0.03	10.17	0.20	2000	80	419	39	1.76	0.14	2.77	0.22	9	18.35	1.52	419.85	34.73	421.47	34.86	1867.43	74.96	114.22	8.83	2156.15	84.76	1.73	0.13	2.73	0.21
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	BX_Ctl	B	X	Control	northern Denmark	northern Norway	0.0349	40	34.7	8.00	0.03	10.17	0.20	2000	80	419	39	1.76	0.14	2.77	0.22	9	18.35	1.52	419.85	34.73	421.47	34.86	1867.43	74.96	114.22	8.83	2156.15	84.76	1.73	0.13	2.73	0.21
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	BW_Ctl	B	W	Control	northern Denmark	northern Norway	0.0393	62	34.7	8.00	0.03	10.17	0.20	2000	80	419	39	1.76	0.14	2.77	0.22	9	18.35	1.52	419.85	34.73	421.47	34.86	1867.43	74.96	114.22	8.83	2156.15	84.76	1.73	0.13	2.73	0.21
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	BD_Ctl	B	D	Control	northern Denmark	northern Denmark	0.0379	67	34.7	8.00	0.03	10.17	0.20	2000	80	419	39	1.76	0.14	2.77	0.22	9	18.35	1.52	419.85	34.73	421.47	34.86	1867.43	74.96	114.22	8.83	2156.15	84.76	1.73	0.13	2.73	0.21
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	YD_Ctl	Y	D	Control	northern Norway	northern Denmark	0.0416	73	34.7	8.00	0.03	10.17	0.20	2000	80	419	39	1.76	0.14	2.77	0.22	9	18.35	1.52	419.85	34.73	421.47	34.86	1867.43	74.96	114.22	8.83	2156.15	84.76	1.73	0.13	2.73	0.21
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	YZ_Ctl	Y	Z	Control	northern Norway	northern Norway	0.0382	106	34.7	8.00	0.03	10.17	0.20	2000	80	419	39	1.76	0.14	2.77	0.22	9	18.35	1.52	419.85	34.73	421.47	34.86	1867.43	74.96	114.22	8.83	2156.15	84.76	1.73	0.13	2.73	0.21
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	YW_Ctl	Y	W	Control	northern Norway	northern Norway	0.0426	71	34.7	8.00	0.03	10.17	0.20	2000	80	419	39	1.76	0.14	2.77	0.22	9	18.35	1.52	419.85	34.73	421.47	34.86	1867.43	74.96	114.22	8.83	2156.15	84.76	1.73	0.13	2.73	0.21
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	AX_Low	A	X	Low pH	northern Denmark	northern Norway	0.0363	23	34.7	7.62	0.11	10.34	0.24	2130	60	1145	296	0.83	0.25	1.30	0.40	9	47.65	12.17	1096.32	279.98	1100.54	281.06	2030.23	57.20	52.11	13.26	2161.93	68.02	0.79	0.20	1.24	0.32
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	AW_Low	A	W	Low pH	northern Denmark	northern Norway	0.0348	34	34.7	7.62	0.11	10.34	0.24	2130	60	1145	296	0.83	0.25	1.30	0.40	9	47.65	12.17	1096.32	279.98	1100.54	281.06	2030.23	57.20	52.11	13.26	2161.93	68.02	0.79	0.20	1.24	0.32
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	AZ_Low	A	Z	Low pH	northern Denmark	northern Norway	0.0331	35	34.7	7.62	0.11	10.34	0.24	2130	60	1145	296	0.83	0.25	1.30	0.40	9	47.65	12.17	1096.32	279.98	1100.54	281.06	2030.23	57.20	52.11	13.26	2161.93	68.02	0.79	0.20	1.24	0.32
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	AB_Low	A	B	Low pH	northern Denmark	northern Denmark	0.0314	56	34.7	7.62	0.11	10.34	0.24	2130	60	1145	296	0.83	0.25	1.30	0.40	9	47.65	12.17	1096.32	279.98	1100.54	281.06	2030.23	57.20	52.11	13.26	2161.93	68.02	0.79	0.20	1.24	0.32
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	BX_Low	B	X	Low pH	northern Denmark	northern Norway	0.0299	67	34.7	7.62	0.11	10.34	0.24	2130	60	1145	296	0.83	0.25	1.30	0.40	9	47.65	12.17	1096.32	279.98	1100.54	281.06	2030.23	57.20	52.11	13.26	2161.93	68.02	0.79	0.20	1.24	0.32
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	BW_Low	B	W	Low pH	northern Denmark	northern Norway	0.0290	73	34.7	7.62	0.11	10.34	0.24	2130	60	1145	296	0.83	0.25	1.30	0.40	9	47.65	12.17	1096.32	279.98	1100.54	281.06	2030.23	57.20	52.11	13.26	2161.93	68.02	0.79	0.20	1.24	0.32
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	BD_Low	B	D	Low pH	northern Denmark	northern Denmark	0.0324	61	34.7	7.62	0.11	10.34	0.24	2130	60	1145	296	0.83	0.25	1.30	0.40	9	47.65	12.17	1096.32	279.98	1100.54	281.06	2030.23	57.20	52.11	13.26	2161.93	68.02	0.79	0.20	1.24	0.32
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	BZ_Low	B	Z	Low pH	northern Denmark	northern Norway	0.0315	40	34.7	7.62	0.11	10.34	0.24	2130	60	1145	296	0.83	0.25	1.30	0.40	9	47.65	12.17	1096.32	279.98	1100.54	281.06	2030.23	57.20	52.11	13.26	2161.93	68.02	0.79	0.20	1.24	0.32
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	BB_Low	B	B	Low pH	northern Denmark	northern Denmark	0.0299	65	34.7	7.62	0.11	10.34	0.24	2130	60	1145	296	0.83	0.25	1.30	0.40	9	47.65	12.17	1096.32	279.98	1100.54	281.06	2030.23	57.20	52.11	13.26	2161.93	68.02	0.79	0.20	1.24	0.32
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	YD_Low	Y	D	Low pH	northern Norway	northern Denmark	0.0329	44	34.7	7.62	0.11	10.34	0.24	2130	60	1145	296	0.83	0.25	1.30	0.40	9	47.65	12.17	1096.32	279.98	1100.54	281.06	2030.23	57.20	52.11	13.26	2161.93	68.02	0.79	0.20	1.24	0.32
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	YZ_Low	Y	Z	Low pH	northern Norway	northern Norway	0.0338	51	34.7	7.62	0.11	10.34	0.24	2130	60	1145	296	0.83	0.25	1.30	0.40	9	47.65	12.17	1096.32	279.98	1100.54	281.06	2030.23	57.20	52.11	13.26	2161.93	68.02	0.79	0.20	1.24	0.32
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	YW_Low	Y	W	Low pH	northern Norway	northern Norway	0.0375	48	34.7	7.62	0.11	10.34	0.24	2130	60	1145	296	0.83	0.25	1.30	0.40	9	47.65	12.17	1096.32	279.98	1100.54	281.06	2030.23	57.20	52.11	13.26	2161.93	68.02	0.79	0.20	1.24	0.32
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	AA_Ctl	A	A	Control	northern Denmark	northern Denmark	0.0368	36	34.7	8.00	0.03	10.17	0.20	2000	80	419	39	1.76	0.14	2.77	0.22	9	18.35	1.52	419.85	34.73	421.47	34.86	1867.43	74.96	114.22	8.83	2156.15	84.76	1.73	0.13	2.73	0.21
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	AA_Low	A	A	Low pH	northern Denmark	northern Denmark	0.0363	40	34.7	7.62	0.11	10.34	0.24	2130	60	1145	296	0.83	0.25	1.30	0.40	9	47.65	12.17	1096.32	279.98	1100.54	281.06	2030.23	57.20	52.11	13.26	2161.93	68.02	0.79	0.20	1.24	0.32
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	BA_Ctl	B	A	Control	northern Denmark	northern Denmark	0.0332	104	34.7	8.00	0.03	10.17	0.20	2000	80	419	39	1.76	0.14	2.77	0.22	9	18.35	1.52	419.85	34.73	421.47	34.86	1867.43	74.96	114.22	8.83	2156.15	84.76	1.73	0.13	2.73	0.21
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	BA_Low	B	A	Low pH	northern Denmark	northern Denmark	0.0329	27	34.7	7.62	0.11	10.34	0.24	2130	60	1145	296	0.83	0.25	1.30	0.40	9	47.65	12.17	1096.32	279.98	1100.54	281.06	2030.23	57.20	52.11	13.26	2161.93	68.02	0.79	0.20	1.24	0.32
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	YA_Ctl	Y	A	Control	northern Norway	northern Denmark	0.0384	102	34.7	8.00	0.03	10.17	0.20	2000	80	419	39	1.76	0.14	2.77	0.22	9	18.35	1.52	419.85	34.73	421.47	34.86	1867.43	74.96	114.22	8.83	2156.15	84.76	1.73	0.13	2.73	0.21
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	YA_Low	Y	A	Low pH	northern Norway	northern Denmark	0.0337	124	34.7	7.62	0.11	10.34	0.24	2130	60	1145	296	0.83	0.25	1.30	0.40	9	47.65	12.17	1096.32	279.98	1100.54	281.06	2030.23	57.20	52.11	13.26	2161.93	68.02	0.79	0.20	1.24	0.32
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	AC_Ctl	A	C	Control	northern Denmark	northern Denmark	0.0409	30	34.7	8.00	0.03	10.17	0.20	2000	80	419	39	1.76	0.14	2.77	0.22	9	18.35	1.52	419.85	34.73	421.47	34.86	1867.43	74.96	114.22	8.83	2156.15	84.76	1.73	0.13	2.73	0.21
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	AC_Low	A	C	Low pH	northern Denmark	northern Denmark	0.0377	28	34.7	7.62	0.11	10.34	0.24	2130	60	1145	296	0.83	0.25	1.30	0.40	9	47.65	12.17	1096.32	279.98	1100.54	281.06	2030.23	57.20	52.11	13.26	2161.93	68.02	0.79	0.20	1.24	0.32
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	BC_Ctl	B	C	Control	northern Denmark	northern Denmark	0.0342	37	34.7	8.00	0.03	10.17	0.20	2000	80	419	39	1.76	0.14	2.77	0.22	9	18.35	1.52	419.85	34.73	421.47	34.86	1867.43	74.96	114.22	8.83	2156.15	84.76	1.73	0.13	2.73	0.21
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	BC_Low	B	C	Low pH	northern Denmark	northern Denmark	0.0299	16	34.7	7.62	0.11	10.34	0.24	2130	60	1145	296	0.83	0.25	1.30	0.40	9	47.65	12.17	1096.32	279.98	1100.54	281.06	2030.23	57.20	52.11	13.26	2161.93	68.02	0.79	0.20	1.24	0.32
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	YC_Ctl	Y	C	Control	northern Norway	northern Denmark	0.0441	82	34.7	8.00	0.03	10.17	0.20	2000	80	419	39	1.76	0.14	2.77	0.22	9	18.35	1.52	419.85	34.73	421.47	34.86	1867.43	74.96	114.22	8.83	2156.15	84.76	1.73	0.13	2.73	0.21
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	YC_Low	Y	C	Low pH	northern Norway	northern Denmark	0.0311	100	34.7	7.62	0.11	10.34	0.24	2130	60	1145	296	0.83	0.25	1.30	0.40	9	47.65	12.17	1096.32	279.98	1100.54	281.06	2030.23	57.20	52.11	13.26	2161.93	68.02	0.79	0.20	1.24	0.32
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	YX_Ctl	Y	X	Control	northern Norway	northern Norway	0.0349	75	34.7	8.00	0.03	10.17	0.20	2000	80	419	39	1.76	0.14	2.77	0.22	9	18.35	1.52	419.85	34.73	421.47	34.86	1867.43	74.96	114.22	8.83	2156.15	84.76	1.73	0.13	2.73	0.21
laboratory	Strongylocentrotus droebachiensis	marinespecies.org	urn:lsid:marinespecies.org:taxname:124321	YX_Low	Y	X	Low pH	northern Norway	northern Norway	0.0309	63	34.7	7.62	0.11	10.34	0.24	2130	60	1145	296	0.83	0.25	1.30	0.40	9	47.65	12.17	1096.32	279.98	1100.54	281.06	2030.23	57.20	52.11	13.26	2161.93	68.02	0.79	0.20	1.24	0.32