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Matthiessen, Birte; Hattich, Giannina S I; Pulina, Silvia; Hansen, Thomas; Reusch, Thorsten B H; Hamer, Jorin (2023): Short-term experiment (7 days) with nine Chaetoceros affinis genotypes at seven nitrate levels [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.957879, In: Matthiessen, B et al. (2023): Experiments on coexisting phytoplankton species Chaetoceros affinis and Emiliania huxleyi in response to nutrients [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.957930

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Abstract:
Measurements of cell size and cell density in a short-term experiment (7 days) of nine Chaetoceros affinis genotypes (http://roscoff-culture-collection.org/; B63: RCC6347, B67: RCC6348, B68: RCC6349, B74: RCC6350) separately at seven nitrate levels 2.5, 5, 7.5, 12.5, 20, 30, and 40 μmol L−1 N) and 2 μmoL-1 phosphate. Silicate concentrations were aligned to reflect a 1:1.5 N:Si ratio. Prior to the experiment each genotype was acclimated over two weeks at initial nutrient concentrations of 1.8 µmol L-1 P, 30 µmol L-1 N, and 40 µmol L-1 Si.
Keyword(s):
cell size; diatoms; evolution; Genotype Sorting; Laboratory experiment; nutrients; phenotypic plasticity; Phytoplankton; Species Sorting; trait variability
Funding:
German Research Foundation (DFG), grant/award no. MA5058/2-2
German Research Foundation (DFG), grant/award no. RE1708/17-2
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Type of studyStudy typeMatthiessen, Birte
2Sampling date/time, experimentDate/time sampling expMatthiessen, Birte
3Day of experimentDOEdayMatthiessen, Birte
4Bottle numberBottle#Matthiessen, Birte
5Treatment: nitrateT:[NO3]-µmol/lMatthiessen, Birte
6GenotypeGenotypeMatthiessen, Birte
7MorphotypeMorphotypeMatthiessen, Birtebased on cell diameter of Chaetoceros affinis, small (<5µm), medium(5-10 µm), large(10-15 µm), giant (>15 µm)
8Chaetoceros affinisC. affinis#/mlMatthiessen, BirteMicroscopy (Zeiss Axiovert 200)
9Chaetoceros affinis, heightC. affinis hµmMatthiessen, BirteMicroscopy (Zeiss Axiovert 200)
10Chaetoceros affinis, diameterC. affinis diamµmMatthiessen, BirteMicroscopy (Zeiss Axiovert 200)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
8660 data points

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