Citation:

PANGAEA.957783

Name: Seawater carbonate chemistry and common sea star (Asterias rubens) coelomic fluid changes
Published: 2023-04-23
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Acid-base regulation; Animalia; Asterias rubens; Benthic animals; Benthos; Biomass/Abundance/Elemental composition; Containers and aquaria (20-1000 L or < 1 m**2); Echinodermata; Laboratory experiment; Laboratory strains; Not applicable; Other studied parameter or process; Oxygen; Single species; Temperature

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Abstract:

Common sea stars (Asterias rubens) are at risk of physiological stress and decline with projected shifts in oceanic conditions. This study assessed changes in coelomic fluid (CF) blood gases, electrolytes, osmolality, and coelomocyte counts in adult common sea stars after exposure to stressors mimicking effects from climate change for 14 days, including decreased pH (−0.4 units, mean: 7.37), hypoxia (target dissolved oxygen ~1.75 mg O2/L, mean: 1.80 mg O2/L), or increased temperature (+10 °C, mean: 17.2 °C) and compared sea star CF electrolytes and osmolality to tank water. Changes in CF blood gases, electrolytes, and/or coelomocyte counts occurred in all treatment groups after stressor exposures, indicating adverse systemic effects with evidence of increased energy expenditure, respiratory or metabolic derangements, and immunosuppression or inflammation. At baseline, CF potassium and osmolality of all groups combined were significantly higher than tank water, and, after exposures, CF potassium was significantly higher in the hypoxia group as compared to tank water. These findings indicate physiological challenges for A. rubens after stressor exposures and, given increased observations of sea star wasting events globally, this provides evidence that sea stars as a broad group are particularly vulnerable to changing oceans.

Keyword(s):

Acid-base regulation; Animalia; Asterias rubens; Benthic animals; Benthos; Biomass/Abundance/Elemental composition; Containers and aquaria (20-1000 L or < 1 m**2); Echinodermata; Laboratory experiment; Laboratory strains; Not applicable; Other studied parameter or process; Oxygen; Single species; Temperature

Supplement to:

Wahltinez, Sarah J; Kroll, Kevin J; Behringer, Donald C; Arnold, Jill E; Whitaker, Brent; Newton, Alisa L; Edmiston, Kristina; Hewson, Ian; Stacy, Nicole I (2023): Common Sea Star (Asterias rubens) Coelomic Fluid Changes in Response to Short-Term Exposure to Environmental Stressors. Fishes, 8(1), 51, https://doi.org/10.3390/fishes8010051

Documentation:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2022): seacarb: seawater carbonate chemistry with R. R package version 3.3.1. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-04-20.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type of study	Study type		Stacy, Nicole I
2	Species, unique identification	Species UID		Stacy, Nicole I
3	Species, unique identification (URI)	Species UID (URI)		Stacy, Nicole I
4	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		Stacy, Nicole I
5	Treatment	Treat		Stacy, Nicole I
6	Time point, descriptive	Time point		Stacy, Nicole I
7	Coelomic fluid, pH	pH (cf)		Stacy, Nicole I
8	Coelomic fluid, pH, standard deviation	pH (cf) std dev	±	Stacy, Nicole I
9	Coelomic fluid, partial pressure of carbon dioxide	pCO2 (cf)	Torr	Stacy, Nicole I
10	Coelomic fluid, partial pressure of carbon dioxide, standard deviation	pCO2 (cf) std dev	±	Stacy, Nicole I
11	Coelomic fluid, partial pressure of oxygen	pO2 (cf)	torr	Stacy, Nicole I
12	Coelomic fluid, partial pressure of oxygen, standard deviation	pO2 (cf) std dev	±	Stacy, Nicole I
13	Coelomic fluid, bicarbonate ion	[HCO3]- (cf)	mmol/l	Stacy, Nicole I
14	Coelomic fluid, bicarbonate ion, standard deviation	[HCO3]- (cf) std dev	±	Stacy, Nicole I
15	Type	Type		Stacy, Nicole I
16	Magnesium	Mg	mmol/l	Stacy, Nicole I
17	Magnesium, standard deviation	Mg std dev	±	Stacy, Nicole I
18	Sodium	Na	mmol/l	Stacy, Nicole I
19	Sodium, standard deviation	Na std dev	±	Stacy, Nicole I
20	Potassium	K	mmol/l	Stacy, Nicole I
21	Potassium, standard deviation	K std dev	±	Stacy, Nicole I
22	Chloride	Cl-	mmol/l	Stacy, Nicole I
23	Chloride, standard deviation	Cl std dev	±	Stacy, Nicole I
24	Calcium	Ca	mmol/l	Stacy, Nicole I
25	Calcium, standard deviation	Ca std dev	±	Stacy, Nicole I
26	Osmolality	Osm	mOsm/kg	Stacy, Nicole I
27	Osmolality, standard deviation	Osm std dev	±	Stacy, Nicole I
28	Total coelomocyte count	TCC	#/ml	Stacy, Nicole I
29	Total coelomocyte count, standard deviation	TCC std dev	±	Stacy, Nicole I
30	Salinity	Sal		Stacy, Nicole I
31	Temperature, water	Temp	°C	Stacy, Nicole I
32	pH	pH		Stacy, Nicole I		NBS scale
33	pH, standard deviation	pH std dev	±	Stacy, Nicole I		NBS scale
34	Alkalinity, total	AT	µmol/kg	Stacy, Nicole I
35	Alkalinity, total, standard deviation	AT std dev	±	Stacy, Nicole I
36	Carbon, inorganic, dissolved	DIC	µmol/kg	Stacy, Nicole I
37	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Stacy, Nicole I
38	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Stacy, Nicole I
39	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Stacy, Nicole I
40	Bicarbonate ion	[HCO3]-	µmol/kg	Stacy, Nicole I
41	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Stacy, Nicole I
42	Carbonate ion	[CO3]2-	µmol/kg	Stacy, Nicole I
43	Carbonate ion, standard deviation	[CO3]2- std dev	±	Stacy, Nicole I
44	Aragonite saturation state	Omega Arg		Stacy, Nicole I
45	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Stacy, Nicole I
46	Calcite saturation state	Omega Cal		Stacy, Nicole I
47	Calcite saturation state, standard deviation	Omega Cal std dev	±	Stacy, Nicole I
48	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
49	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
50	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
51	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
52	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
53	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
54	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
55	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
56	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
57	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

538 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Study type	2 Species UID	3 Species UID (URI)	4 Species UID (Semantic URI)	5 Treat	6 Time point	7 pH (cf)	8 pH (cf) std dev [±]	9 pCO2 (cf) [Torr]	10 pCO2 (cf) std dev [±]	11 pO2 (cf) [torr]	12 pO2 (cf) std dev [±]	13 [HCO3]- (cf) [mmol/l]	14 [HCO3]- (cf) std dev [±]	15 Type	16 Mg [mmol/l]	17 Mg std dev [±]	18 Na [mmol/l]	19 Na std dev [±]	20 K [mmol/l]	21 K std dev [±]	22 Cl- [mmol/l]	23 Cl std dev [±]	24 Ca [mmol/l]	25 Ca std dev [±]	26 Osm [mOsm/kg]	27 Osm std dev [±]	28 TCC [#/ml]	29 TCC std dev [±]	30 Sal	31 Temp [°C]	32 pH (NBS scale)	33 pH std dev [±] (NBS scale)	34 AT [µmol/kg]	35 AT std dev [±]	36 DIC [µmol/kg]	37 DIC std dev [±]	38 pCO2water_SST_wet [µatm]	39 pCO2 std dev [±]	40 [HCO3]- [µmol/kg]	41 [HCO3]- std dev [±]	42 [CO3]2- [µmol/kg]	43 [CO3]2- std dev [±]	44 Omega Arg	45 Omega Arg std dev [±]	46 Omega Cal	47 Omega Cal std dev [±]	48 CSC flag	49 pH (total scale)	50 CO2 [µmol/kg]	51 fCO2water_SST_wet [µatm]	52 pCO2water_SST_wet [µatm]	53 [HCO3]- [µmol/kg]	54 [CO3]2- [µmol/kg]	55 AT [µmol/kg]	56 Omega Arg	57 Omega Cal
laboratory	Asterias rubens	marinespecies.org	urn:lsid:marinespecies.org:taxname:123776	pH	T0	7.42	0.08	2.2	0.2	103	9				52	3	427	6	9.4	0.5	495	7	9.7	0.1	959	8.0	15590	15880	33.1	7.65	7.78	0.09	1640	440	2180	100	783.5	177.7	2070	100	70	0.010	1.03	0.17	1.63	0.26	27	7.68	46.53	968.98	972.85	2080.67	52.80	2213.20	0.80	1.27
laboratory	Asterias rubens	marinespecies.org	urn:lsid:marinespecies.org:taxname:123776	pH	T1	7.31	0.08	2.4	0.2	113	6	1.6	0.3		54	7	416	10	9.2	0.3	482	12	9.4	0.2	941	4.0	5780	7070	33.1	7.65	7.45	0.05	1390	480	2060	170	1548.2	273.2	1960	160	30	0.003	0.45	0.04	0.72	0.06	27	7.35	92.91	1934.72	1942.44	1944.01	23.08	2003.20	0.35	0.56
laboratory	Asterias rubens	marinespecies.org	urn:lsid:marinespecies.org:taxname:123776	Hypoxia	T0	7.45	0.10	2.1	0.2	107	6	1.9	0.3		51	6	425	3	9.4	0.1	492	4	9.5	0.2	944	4.0	8900	13600
laboratory	Asterias rubens	marinespecies.org	urn:lsid:marinespecies.org:taxname:123776	Hypoxia	T1	7.65	0.07	1.8	0.2	55	2	2.7	0.4		47	6	402	57	9.5	1.0	468	64	9.2	1.1	939	6.0	14100	22150
laboratory	Asterias rubens	marinespecies.org	urn:lsid:marinespecies.org:taxname:123776	Temperature	T0	7.56	0.06	2.1	0.2	114	8	2.4	0.2		65	13	451	8	10.0	0.1	521	8	10.0	0.1	984	5.0	8580	12430
laboratory	Asterias rubens	marinespecies.org	urn:lsid:marinespecies.org:taxname:123776	Temperature	T1	7.35	0.08	3.0	0.2	98	7	2.0	0.3		54	4	435	4	9.6	0.3	505	4	9.8	0.1	968	25.0	11780	14660
laboratory	Asterias rubens	marinespecies.org	urn:lsid:marinespecies.org:taxname:123776	Control	T0	7.54	0.05	2.0	0.1	113	6	2.2	0.3		51	7	442	4	9.8	0.1	511	5	9.9	0.3	986	6.6	1140	770	33.1	7.65	7.85	0.04	1660	380	2180	120	655.6	88.0	2070	110	80	0.010	1.19	0.11	1.89	0.17	27	7.75	39.56	823.83	827.11	2078.47	61.97	2233.70	0.94	1.49
laboratory	Asterias rubens	marinespecies.org	urn:lsid:marinespecies.org:taxname:123776	Control	T1	7.51	0.11	2.3	0.2	112	8	2.4	0.5		54	2	432	4	9.4	0.1	501	7	9.6	0.2	944	3.4	1130	370	33.1	7.65	7.82	0.06	1210	400	2000	140	655.0	107.0	1900	130	70	0.010	1.02	0.15	1.61	0.23	27	7.72	38.92	810.53	813.76	1908.00	53.08	2043.50	0.81	1.28
laboratory	Asterias rubens	marinespecies.org	urn:lsid:marinespecies.org:taxname:123776		T0									SeaStar	54		338		9.6		506		9.8		969
laboratory	Asterias rubens	marinespecies.org	urn:lsid:marinespecies.org:taxname:123776		T0									Tank	46		394		8.2		452		8.9		866
laboratory	Asterias rubens	marinespecies.org	urn:lsid:marinespecies.org:taxname:123776	pH	T1									SeaStar	51		428		9.4		496		9.6		963				33.1	7.65	7.45	0.05	1390	480	2060	170	1548.2	273.2	1960	160	30	0.003	0.45	0.04	0.72	0.06	27	7.35	92.91	1934.72	1942.44	1944.01	23.08	2003.20	0.35	0.56
laboratory	Asterias rubens	marinespecies.org	urn:lsid:marinespecies.org:taxname:123776	pH	T1									Tank	42		337		7.0		391		7.8		824				33.1	7.65	7.45	0.05	1390	480	2060	170	1548.2	273.2	1960	160	30	0.003	0.45	0.04	0.72	0.06	27	7.35	92.91	1934.72	1942.44	1944.01	23.08	2003.20	0.35	0.56
laboratory	Asterias rubens	marinespecies.org	urn:lsid:marinespecies.org:taxname:123776	Hypoxia	T1									SeaStar	49		435		9.6		504		9.9		962
laboratory	Asterias rubens	marinespecies.org	urn:lsid:marinespecies.org:taxname:123776	Hypoxia	T1									Tank	47		409		8.6		452		8.8		911
laboratory	Asterias rubens	marinespecies.org	urn:lsid:marinespecies.org:taxname:123776	Temperature	T1									SeaStar	51		441		10.0		512		9.9		975
laboratory	Asterias rubens	marinespecies.org	urn:lsid:marinespecies.org:taxname:123776	Temperature	T1									Tank	47		410		8.6		476		9.2		944
laboratory	Asterias rubens	marinespecies.org	urn:lsid:marinespecies.org:taxname:123776	Control	T1									SeaStar	57		440		9.8		509		9.9		969				33.1	7.65	7.82	0.06	1210	400	2000	140	655.0	107.0	1900	130	70	0.010	1.02	0.15	1.61	0.23	27	7.72	38.92	810.53	813.76	1908.00	53.08	2043.50	0.81	1.28
laboratory	Asterias rubens	marinespecies.org	urn:lsid:marinespecies.org:taxname:123776	Control	T1									Tank	50		401		8.4		471		9.1		967				33.1	7.65	7.82	0.06	1210	400	2000	140	655.0	107.0	1900	130	70	0.010	1.02	0.15	1.61	0.23	27	7.72	38.92	810.53	813.76	1908.00	53.08	2043.50	0.81	1.28