Citation:

PANGAEA.957780

Name: Seawater carbonate chemistry and phenotypic Plasticity in Crassostrea virginica Larvae
Published: 2023-04-23
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea virginica; Growth/Morphology; Laboratory experiment; Mollusca; Mortality/Survival; North Atlantic; Pelagos; Single species; Temperate; Zooplankton

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Abstract:

Ocean acidification (OA) is a major threat to marine calcifiers, and little is known regarding acclimation to OA in bivalves. This study combined physiological assays with next-generation sequencing to assess the potential for recovery from and acclimation to OA in the eastern oyster (Crassostrea virginica) and identify molecular mechanisms associated with resilience. In a reciprocal transplant experiment, larvae transplanted from elevated pCO2 (~1400 ppm) to ambient pCO2 (~350 ppm) demonstrated significantly lower mortality and larger size post-transplant than oysters remaining under elevated pCO2 and had similar mortality compared to those remaining in ambient conditions. The recovery after transplantation to ambient conditions demonstrates the ability for larvae to rebound and suggests phenotypic plasticity and acclimation. Transcriptomic analysis supported this hypothesis as genes were differentially regulated under OA stress. Transcriptomic profiles of transplanted and non-transplanted larvae terminating in the same final pCO2 converged, further supporting the idea that acclimation underlies resilience. The functions of differentially expressed genes included cell differentiation, development, biomineralization, ion exchange, and immunity. Results suggest acclimation as a mode of resilience to OA. In addition, the identification of genes associated with resilience can serve as a valuable resource for the aquaculture industry, as these could enable marker-assisted selection of OA-resilient stocks.

Keyword(s):

Animalia; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea virginica; Growth/Morphology; Laboratory experiment; Mollusca; Mortality/Survival; North Atlantic; Pelagos; Single species; Temperate; Zooplankton

Supplement to:

Barbosa, Michelle; Schwaner, Caroline; Espinosa, Emmanuelle Pales; Allam, Bassem (2022): A Transcriptomic Analysis of Phenotypic Plasticity in Crassostrea virginica Larvae under Experimental Acidification. Genes, 13(9), 1529, https://doi.org/10.3390/genes13091529

Documentation:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2022): seacarb: seawater carbonate chemistry with R. R package version 3.3.1. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Median Latitude: 41.168275 * Median Longitude: -72.212275 * South-bound Latitude: 40.884000 * West-bound Longitude: -73.343200 * North-bound Latitude: 41.930500 * East-bound Longitude: -70.031000

Event(s):

Mount_Sinai_Harbor * Latitude: 40.957700 * Longitude: -73.033500 * Method/Device: Experiment (EXP)

Northport_NY * Latitude: 40.900900 * Longitude: -73.343200 * Method/Device: Experiment (EXP)

Southampton_NY * Latitude: 40.884000 * Longitude: -72.441400 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-04-23.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type of study	Study type		Allam, Bassem
2	Species, unique identification	Species UID		Allam, Bassem
3	Species, unique identification (URI)	Species UID (URI)		Allam, Bassem
4	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		Allam, Bassem
5	Treatment	Treat		Allam, Bassem		first letter signifies original pCO2 treatment (A: ambient, first letter signifies original pCO2 treatment (A: ambient, E: elevated); second letter signifies final pCO2 treatment
6	Identification	ID		Allam, Bassem		tank
7	Length	l	µm	Allam, Bassem		Length for 7 day old oyster larva
8	Mortality	Mortality	%	Allam, Bassem		for 7 day old oyster larva
9	Mortality	Mortality	%	Allam, Bassem		1 week post-transplant
10	Length	l	µm	Allam, Bassem		for 15 day old oyster larvae transplanted
11	Salinity	Sal		Allam, Bassem
12	Temperature, water	Temp	°C	Allam, Bassem
13	pH	pH		Allam, Bassem	Potentiometric	total scale
14	pH, standard deviation	pH std dev	±	Allam, Bassem	Potentiometric	total scale
15	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	ppmv	Allam, Bassem	Calculated using seacarb
16	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Allam, Bassem	Calculated using seacarb
17	Aragonite saturation state	Omega Arg		Allam, Bassem	Calculated using seacarb
18	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Allam, Bassem	Calculated using seacarb
19	Calcite saturation state	Omega Cal		Allam, Bassem	Calculated using seacarb
20	Calcite saturation state, standard deviation	Omega Cal std dev	±	Allam, Bassem	Calculated using seacarb
21	Carbon, inorganic, dissolved	DIC	µmol/kg	Allam, Bassem	Coulometric titration
22	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Allam, Bassem	Coulometric titration
23	Carbonate ion	[CO3]2-	µmol/kg	Allam, Bassem	Calculated using seacarb
24	Carbonate ion, standard deviation	[CO3]2- std dev	±	Allam, Bassem	Calculated using seacarb
25	Alkalinity, total	AT	µmol/kg	Allam, Bassem	Calculated using seacarb
26	Alkalinity, total, standard deviation	AT std dev	±	Allam, Bassem	Calculated using seacarb
27	pH	pH		Allam, Bassem	Potentiometric	total scale, post-transplant
28	pH, standard deviation	pH std dev	±	Allam, Bassem	Potentiometric	total scale, post-transplant
29	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	ppmv	Allam, Bassem	Calculated using seacarb	post-transplant
30	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Allam, Bassem	Calculated using seacarb	post-transplant
31	Aragonite saturation state	Omega Arg		Allam, Bassem	Calculated using seacarb	post-transplant
32	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Allam, Bassem	Calculated using seacarb	post-transplant
33	Calcite saturation state	Omega Cal		Allam, Bassem	Calculated using seacarb	post-transplant
34	Calcite saturation state, standard deviation	Omega Cal std dev	±	Allam, Bassem	Calculated using seacarb	post-transplant
35	Carbon, inorganic, dissolved	DIC	µmol/kg	Allam, Bassem	Coulometric titration	post-transplant
36	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Allam, Bassem	Coulometric titration	post-transplant
37	Carbonate ion	[CO3]2-	µmol/kg	Allam, Bassem	Calculated using seacarb	post-transplant
38	Carbonate ion, standard deviation	[CO3]2- std dev	±	Allam, Bassem	Calculated using seacarb	post-transplant
39	Alkalinity, total	AT	µmol/kg	Allam, Bassem	Calculated using seacarb	post-transplant
40	Alkalinity, total, standard deviation	AT std dev	±	Allam, Bassem	Calculated using seacarb	post-transplant
41	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
48	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
49	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
50	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	post-transplant
51	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	post-transplant
52	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	post-transplant
53	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	post-transplant
54	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	post-transplant
55	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	post-transplant
56	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	post-transplant
57	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	post-transplant

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1912 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Study type	2 Species UID	3 Species UID (URI)	4 Species UID (Semantic URI)	5 Treat (first letter signifies origin...)	6 ID (tank)	7 l [µm] (Length for 7 day old oyster l...)	8 Mortality [%] (for 7 day old oyster larva)	9 Mortality [%] (1 week post-transplant)	10 l [µm] (for 15 day old oyster larvae ...)	11 Sal	12 Temp [°C]	13 pH (total scale, Potentiometric)	14 pH std dev [±] (total scale, Potentiometric)	15 pCO2water_SST_wet [ppmv] (Calculated using seacarb)	16 pCO2 std dev [±] (Calculated using seacarb)	17 Omega Arg (Calculated using seacarb)	18 Omega Arg std dev [±] (Calculated using seacarb)	19 Omega Cal (Calculated using seacarb)	20 Omega Cal std dev [±] (Calculated using seacarb)	21 DIC [µmol/kg] (Coulometric titration)	22 DIC std dev [±] (Coulometric titration)	23 [CO3]2- [µmol/kg] (Calculated using seacarb)	24 [CO3]2- std dev [±] (Calculated using seacarb)	25 AT [µmol/kg] (Calculated using seacarb)	26 AT std dev [±] (Calculated using seacarb)	27 pH (total scale, post-transplant,...)	28 pH std dev [±] (total scale, post-transplant,...)	29 pCO2water_SST_wet [ppmv] (post-transplant, Calculated u...)	30 pCO2 std dev [±] (post-transplant, Calculated u...)	31 Omega Arg (post-transplant, Calculated u...)	32 Omega Arg std dev [±] (post-transplant, Calculated u...)	33 Omega Cal (post-transplant, Calculated u...)	34 Omega Cal std dev [±] (post-transplant, Calculated u...)	35 DIC [µmol/kg] (post-transplant, Coulometric ...)	36 DIC std dev [±] (post-transplant, Coulometric ...)	37 [CO3]2- [µmol/kg] (post-transplant, Calculated u...)	38 [CO3]2- std dev [±] (post-transplant, Calculated u...)	39 AT [µmol/kg] (post-transplant, Calculated u...)	40 AT std dev [±] (post-transplant, Calculated u...)	41 CSC flag (Calculated using seacarb afte...)	42 CO2 [µmol/kg] (Calculated using seacarb afte...)	43 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	44 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	45 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	46 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	47 AT [µmol/kg] (Calculated using seacarb afte...)	48 Omega Arg (Calculated using seacarb afte...)	49 Omega Cal (Calculated using seacarb afte...)	50 CO2 [µmol/kg] (post-transplant, Calculated u...)	51 fCO2water_SST_wet [µatm] (post-transplant, Calculated u...)	52 pCO2water_SST_wet [µatm] (post-transplant, Calculated u...)	53 [HCO3]- [µmol/kg] (post-transplant, Calculated u...)	54 [CO3]2- [µmol/kg] (post-transplant, Calculated u...)	55 AT [µmol/kg] (post-transplant, Calculated u...)	56 Omega Arg (post-transplant, Calculated u...)	57 Omega Cal (post-transplant, Calculated u...)
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	Ambient	1	54.96	5.69			28	20	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95															9	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	Ambient	2	55.13	3.99			28	20	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95															9	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	Ambient	3	53.15	4.17			28	20	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95															9	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	Ambient	4	53.55	3.61			28	20	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95															9	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	Elevated	1	50.62	14.21			28	20	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61															9	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	Elevated	2	53.39	7.00			28	20	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61															9	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	Elevated	3	53.02	10.53			28	20	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61															9	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	Elevated	4	51.42	10.03			28	20	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61															9	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	AA	1			30.71		28	20	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	9	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	AA	2			23.73		28	20	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	9	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	AA	3			31.51		28	20	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	9	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	AA	4			13.10		28	20	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	9	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	AE	1			45.00		28	20	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	9	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	AE	2			31.63		28	20	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	9	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	AE	3			65.74		28	20	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	9	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	AE	4			50.00		28	20	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	9	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	EE	1			52.38		28	20	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	9	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	EE	2			32.50		28	20	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	9	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	EE	3			34.78		28	20	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	9	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	EE	4			59.32		28	20	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	9	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	EA	1			28.81		28	20	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	9	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	EA	2			19.13		28	20	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	9	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	EA	3			18.90		28	20	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	9	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	EA	4			21.03		28	20	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	9	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	AA	1				129.54	28	20	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	9	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	AA	2				127.59	28	20	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	9	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	AA	3				128.86	28	20	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	9	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	AA	4				129.38	28	20	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	9	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	AE	1				113.08	28	20	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	9	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	AE	2				119.18	28	20	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	9	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	AE	3				117.82	28	20	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	9	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	AE	4				117.87	28	20	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	9	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	EE	1				113.64	28	20	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	9	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	EE	2				114.19	28	20	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	9	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	EE	3				113.50	28	20	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	9	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	EE	4				114.30	28	20	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	9	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	EA	1				117.63	28	20	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	9	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	EA	2				116.67	28	20	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	9	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	EA	3				117.48	28	20	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	9	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68
laboratory	Crassostrea virginica	marinespecies.org	urn:lsid:marinespecies.org:taxname:140657	EA	4				117.93	28	20	7.49	0.09	1600.23	346.23	0.83	0.15	1.28	0.24	2060.09	44.85	51.61	9.49	2084.47	46.61	8.14	0.08	322.57	78.74	3.36	0.54	5.20	0.83	1981.30	35.41	209.07	33.58	2268.11	67.95	9	52.97	1573.64	1579.00	1961.33	45.79	2073.25	0.73	1.15	10.79	320.51	321.61	1784.41	186.10	2233.15	2.98	4.68