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Heuer, Rachael M; Wang, Yadong; Pasparakis, Christina; Zhang, Wenlong; Scholey, Vernon; Margulies, Daniel; Grosell, Martin (2023): Seawater carbonate chemistry and metabolic rate and nitrogenous waste handling in the early life stages of yellowfin tuna (Thunnus albacares) [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.957625

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Abstract:
Ocean acidification is predicted to have a wide range of impacts on fish, but there has been little focus on broad-ranging pelagic fish species. Early life stages of fish are thought to be particularly susceptible to CO2 exposure, since acid-base regulatory faculties may not be fully developed. We obtained yellowfin tuna (Thunnus albacares) from a captive spawning broodstock population and exposed them to control or 1900 μatm CO2 through the first three days of development as embryos transitioned into yolk sac larvae. Metabolic rate, yolk sac depletion, and oil globule depletion were measured to assess overall energy usage. To determine if CO2 altered protein catabolism, tissue nitrogen content and nitrogenous waste excretion were quantified. CO2 exposure did not significantly impact embryonic metabolic rate, yolk sac depletion, or oil globule depletion, however, there was a significant decrease in metabolic rate at the latest measured yolk sac larval stage (36 h post fertilization). CO2-exposure led to a significant increase in nitrogenous waste excretion in larvae, but there were no differences in nitrogen tissue accumulation. Nitrogenous waste accumulated in embryos as they developed but decreased after hatch, coinciding with a large increase in nitrogenous waste excretion and increased metabolic rate in newly hatched larvae. Our results provide insight into how yellowfin tuna are impacted by increases in CO2 in early development, but more research with higher levels of replication is needed to better understand long-term impacts and acid-base regulatory mechanisms in this important pelagic fish.
Keyword(s):
Animalia; Chordata; Containers and aquaria (20-1000 L or < 1 m**2); Laboratory experiment; Laboratory strains; Nekton; Not applicable; Other metabolic rates; Pelagos; Reproduction; Respiration; Single species; Thunnus albacares
Supplement to:
Heuer, Rachael M; Wang, Yadong; Pasparakis, Christina; Zhang, Wenlong; Scholey, Vernon; Margulies, Daniel; Grosell, Martin (2023): Effects of elevated CO2 on metabolic rate and nitrogenous waste handling in the early life stages of yellowfin tuna (Thunnus albacares). Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 280, 111398, https://doi.org/10.1016/j.cbpa.2023.111398
Source:
Heuer, Rachael M; Wang, Yadong; Pasparakis, Christina; Scholey, Vernon; Margulies, Daniel; Grosell, Martin (2020): Effects of elevated CO2 on ammonia excretion, metabolic rate, specific gravity, and yolk sac depletion in yellowfin tuna (Thunnus albacares) embryos and larvae. Harte Research Institute, https://doi.org/10.7266/7Z48R4MC
Documentation:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2022): seacarb: seawater carbonate chemistry with R. R package version 3.3.1. https://cran.r-project.org/web/packages/seacarb/index.html
Project(s):
Ocean Acidification International Coordination Centre (OA-ICC)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-04-18.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Type of studyStudy typeHeuer, Rachael M
2Species, unique identificationSpecies UIDHeuer, Rachael M
3Species, unique identification (URI)Species UID (URI)Heuer, Rachael M
4Species, unique identification (Semantic URI)Species UID (Semantic URI)Heuer, Rachael M
5Time in hoursTimehHeuer, Rachael Mpost fertilization
6TreatmentTreatHeuer, Rachael M
7ReplicateReplHeuer, Rachael M
8Ammonia per individualNH3/indpmol/#Heuer, Rachael M
9Urea per individualUrea/indpmol/#Heuer, Rachael M
10Total nitrogen per individualTN/indpmol/#Heuer, Rachael M
11Ammonia excretion per individualNH3 exc/indpmol/#/minHeuer, Rachael M
12Urea excretion rate per individualUrea exc/indpmol/#/minHeuer, Rachael M
13Total nitrogen excretion rate per individualTN exc/indpmol/#/minHeuer, Rachael M
14IdentificationIDHeuer, Rachael MTrial
15Oxygen consumption per individualO2 con/indnmol/#/minHeuer, Rachael M
16IdentificationIDHeuer, Rachael MImage
17IdentificationIDHeuer, Rachael MMeasurement
18SizeSizepixelHeuer, Rachael MArea
19RatioRatioHeuer, Rachael MOil globule size to embryo size
20RatioRatioHeuer, Rachael MYolk sac size to embyro size
21Temperature, waterTemp°CHeuer, Rachael M
22Temperature, water, standard errorT std e±Heuer, Rachael M
23SalinitySalHeuer, Rachael M
24Salinity, standard errorSal std e±Heuer, Rachael M
25Oxygen, dissolvedDOmg/lHeuer, Rachael M
26Oxygen, dissolved, standard errorDO std e±Heuer, Rachael M
27pHpHHeuer, Rachael MNBS scale
28pH, standard errorpH std e±Heuer, Rachael M
29Carbon, inorganic, dissolvedDICµmol/kgHeuer, Rachael M
30Carbon, inorganic, dissolved, standard errorDIC std e±Heuer, Rachael M
31Alkalinity, totalATµmol/kgHeuer, Rachael M
32Alkalinity, total, standard errorAT std e±Heuer, Rachael M
33Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmHeuer, Rachael M
34Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard errorpCO2water_SST_wet std e±Heuer, Rachael M
35Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
36pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)Total scale
37Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
38Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
39Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
40Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
41Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
42Alkalinity, totalATµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
43Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
44Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
18715 data points

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