Citation:

PANGAEA.957342

Name: Seawater carbonate chemistry and nitrogen fixation by phosphorus-limited Trichodesmium
Published: 2023-04-07
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Bacteria; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Cyanobacteria; Entire community; Gene expression (incl. proteomics); Growth/Morphology; Laboratory experiment; Laboratory strains; Macro-nutrients; Micro-nutrients; North Pacific; Not applicable; Open ocean; Other metabolic rates; Other studied parameter or process; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Trichodesmium erythraeum; Tropical

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Abstract:

Growth of the prominent nitrogen-fixing cyanobacterium Trichodesmium is often limited by phosphorus availability in the ocean. How nitrogen fixation by phosphorus-limited Trichodesmium may respond to ocean acidification remains poorly understood. Here, we use phosphate-limited chemostat experiments to show that acidification enhanced phosphorus demands and decreased phosphorus-specific nitrogen fixation rates in Trichodesmium. The increased phosphorus requirements were attributed primarily to elevated cellular polyphosphate contents, likely for maintaining cytosolic pH homeostasis in response to acidification. Alongside the accumulation of polyphosphate, decreased NADP(H):NAD(H) ratios and impaired chlorophyll synthesis and energy production were observed under acidified conditions. Consequently, the negative effects of acidification were amplified compared to those demonstrated previously under phosphorus sufficiency. Estimating the potential implications of this finding, using outputs from the Community Earth System Model, predicts that acidification and dissolved inorganic and organic phosphorus stress could synergistically cause an appreciable decrease in global Trichodesmium nitrogen fixation by 2100.

Keyword(s):

Bacteria; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Cyanobacteria; Entire community; Gene expression (incl. proteomics); Growth/Morphology; Laboratory experiment; Laboratory strains; Macro-nutrients; Micro-nutrients; North Pacific; Not applicable; Open ocean; Other metabolic rates; Other studied parameter or process; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Trichodesmium erythraeum; Tropical

Supplement to:

Zhang, Futing; Wen, Zuozhu; Wang, Shanlin; Tang, Weiyi; Luo, Ya-Wei; Kranz, Sven A; Hong, Haizheng; Shi, Dalin (2022): Phosphate limitation intensifies negative effects of ocean acidification on globally important nitrogen fixing cyanobacterium. Nature Communications, 13(1), 6730, https://doi.org/10.1038/s41467-022-34586-x

Documentation:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2022): seacarb: seawater carbonate chemistry with R. R package version 3.3.1. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Median Latitude: 19.096197 * Median Longitude: 116.342158 * South-bound Latitude: 16.525000 * West-bound Longitude: 115.000000 * North-bound Latitude: 20.798000 * East-bound Longitude: 118.460300

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-04-07.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Shi, Dalin		study
2	Species, unique identification	Species UID		Shi, Dalin
3	Species, unique identification (URI)	Species UID (URI)		Shi, Dalin
4	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		Shi, Dalin
5	Treatment	Treat		Shi, Dalin
6	Replicate	Repl		Shi, Dalin
7	Photosynthetic carbon fixation rate per cell	C fix/cell	fmol/#/h	Shi, Dalin
8	Nitrogen fixation rate, per cell	N2 fix/cell	fmol/#/h	Shi, Dalin
9	Particulate organic carbon, per cell	POC/cell	pmol/#	Shi, Dalin
10	Particulate organic nitrogen per cell	PON/cell	pmol/#	Shi, Dalin
11	Particulate organic phosphorus per cell	POP/cell	pmol/#	Shi, Dalin
12	Polyphosphate as femto-equivalents of the standard per cell	Polyp/cell	feg/#	Shi, Dalin
13	Reduced nicotinamide adenine dinucleotide	NAD(H)	fmol/#	Shi, Dalin
14	Nicotinamide adenine dinucleotide phosphate	NADP(H)	fmol/#	Shi, Dalin
15	Nicotinamide adenine dinucleotide phosphate/Reduced nicotinamide adenine dinucleotide ratio	NADP(H)/NAD(H)		Shi, Dalin
16	Gene expression	Gene expression		Shi, Dalin		ppnk (tery_1770)
17	Gene expression	Gene expression		Shi, Dalin		hemN
18	Gene expression	Gene expression		Shi, Dalin		hemY
19	Gene expression	Gene expression		Shi, Dalin		chlI
20	Gene expression	Gene expression		Shi, Dalin		chlB
21	Gene expression	Gene expression		Shi, Dalin		atpH
22	Gene expression	Gene expression		Shi, Dalin		atpE
23	Adenosine 5-Triphosphate, per cell	ATP/cell	fmol/#	Shi, Dalin
24	Glutamate per cell	Glu/cell	fmol/#	Shi, Dalin
25	Carbon, organic, particulate/Phosphorus, organic, particulate ratio	POC/POP		Shi, Dalin
26	Nitrogen, organic, particulate/Phosphorus, organic, particulate ratio	PON/POP		Shi, Dalin
27	Photosynthetic carbon fixation rate	C fix	mmol/mol/h	Shi, Dalin		per mol P
28	Photosynthetic carbon fixation rate	C fix	mmol/mol/h	Shi, Dalin		per mol C
29	Nitrogen fixation rate	N2 fix	mmol/mol/h	Shi, Dalin		per mol P
30	Nitrogen fixation rate	N2 fix	mmol/mol/h	Shi, Dalin		per mol C
31	Nitrogen fixation rate	N2 fix	mmol/mol/h	Shi, Dalin		per mol N
32	Lipids per cell	Lipids/cell	fmol/#	Shi, Dalin
33	Ribonucleic acid per cell	RNA/cell	fmol/#	Shi, Dalin
34	Cell, length	Cell l	µm	Shi, Dalin
35	Cell, length, standard deviation	Cell l std dev	±	Shi, Dalin
36	Cell, width	Cell w	µm	Shi, Dalin
37	Cell, width, standard deviation	Cell w std dev	±	Shi, Dalin
38	Day of experiment	DOE	day	Shi, Dalin
39	Chlorophyll a per cell	Chl a/cell	pg/#	Shi, Dalin
40	Cell density	Cells	#/ml	Shi, Dalin
41	Phosphorus, reactive soluble	SRP	µmol/l	Shi, Dalin
42	Time point, descriptive	Time point		Shi, Dalin
43	Alkaline phosphatase, para-Nitrophenylphosphate per cell	ALKP pNPP/cell	pmol/#/h	Shi, Dalin
44	Gene expression	Gene expression		Shi, Dalin		phnD1 (or ptxA)
45	Gene expression	Gene expression		Shi, Dalin		phnC1 (or ptxB)
46	Gene expression	Gene expression		Shi, Dalin		phnE1 (or ptxC)
47	Gene expression	Gene expression		Shi, Dalin		ptxD
48	Station label	Station		Shi, Dalin
49	LONGITUDE	Longitude		Shi, Dalin		Geocode
50	LATITUDE	Latitude		Shi, Dalin		Geocode
51	Nitrogen fixation rate	N2 fix	nmol/l/day	Shi, Dalin
52	Gene copies	GCN	#/µl	Shi, Dalin		Trichodesmium
53	Gene copies	GCN	#/µl	Shi, Dalin		UCYN-A1
54	Gene copies	GCN	#/µl	Shi, Dalin		UCYN-A2
55	Gene copies	GCN	#/µl	Shi, Dalin		UCYN-B
56	Gene copies	GCN	#/µl	Shi, Dalin		het-1
57	Gene copies	GCN	#/µl	Shi, Dalin		Gamma-proteobacteria
58	Relative transcription	Rel transcription		Shi, Dalin		ppnKTen/ rnpBTen average
59	Relative transcription	Rel transcription		Shi, Dalin		ppnKTery/ rnpBTery
60	Relative transcription	Rel transcription		Shi, Dalin		sphXTen/ rnpBTen
61	Relative transcription	Rel transcription		Shi, Dalin		sphXTery/ rnpBTery
62	Temperature, water	Temp	°C	Shi, Dalin
63	Salinity	Sal		Shi, Dalin
64	Temperature, water	Temp	°C	Shi, Dalin		input
65	Temperature, water	Temp	°C	Shi, Dalin		output
66	pH	pH		Shi, Dalin		total scale
67	pH	pH		Shi, Dalin		input, total scale
68	Carbon, inorganic, dissolved	DIC	µmol/kg	Shi, Dalin
69	Alkalinity, total	AT	µmol/kg	Shi, Dalin
70	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Shi, Dalin
71	Chlorophyll a	Chl a	µg/l	Shi, Dalin
72	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
73	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
74	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
75	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
76	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
77	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
78	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
79	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
80	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

6821 data points

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