Citation:

PANGAEA.956816

Name: Seawater carbonate chemistry and wild cleaner fish behaviour
Published: 2023-03-22
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Behaviour; Bottles or small containers/Aquaria (<20 L); Chordata; Coast and continental shelf; Laboratory experiment; Labroides dimidiatus; Nekton; Pelagos; Single species; South Pacific; Tropical

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Abstract:

Ocean acidification is considered to affect fish behaviour through the disruption of GABAergic neurotransmission in controlled laboratory conditions, but less is known of the GABAergic role on fish behavioural performance in the wild. Most coral reef fishes engage in complex cleaning interactions, where they benefit from ectoparasite removal and stress relief. Here, we tested whether potential ocean acidification impairment of wild cleaning interactions, between the cleaner fish Labroides dimidiatus and its clients, can be explained by the GABAAR model. We used, the GABAA receptor agonist (muscimol) and antagonist (gabazine) for the first time in the wild and tested their effects on cleaning behaviour in Moorea Island (French Polynesia) to address natural interactions and recovery capacity. After exposure to expected ocean acidification conditions, the proportion of time spent advertising cleaning services, a measure of motivation to interact, dropped significantly relative to controls. Furthermore, the GABAergic antagonist gabazine recovered most CO2-induced behavioural alterations to control levels, consistent with the GABAAR model of altered Cl− flux in ocean acidification-exposed fish. However, muscimol treatment only produced the same behavioural alterations found with CO2 exposure in time spent advertising cleaning. Our results support the evidence that ocean acidification alters some components of cleaning behaviour through GABAA receptor modulation with potential cascading effects on coral reef health and structure.

Keyword(s):

Animalia; Behaviour; Bottles or small containers/Aquaria (<20 L); Chordata; Coast and continental shelf; Laboratory experiment; Labroides dimidiatus; Nekton; Pelagos; Single species; South Pacific; Tropical

Supplement to:

Paula, José Ricardo; Cascalheira, Ligia; Oliveira, Raul; Otjacques, Eve; Frazao-Santos, Catarina; Beldade, Ricardo; Mills, Suzanne; Rosa, Rui (2023): GABAergic role in the disruption of wild cleaner fish behaviour under high CO2. Animal Behaviour, 195, 77-84, https://doi.org/10.1016/j.anbehav.2022.11.003

Original version:

Paula, José Ricardo; Cascalheira, Ligia; Oliveira, Raul; Otjacques, Eve; Frazao-Santos, Catarina; Beldade, Ricardo; Mills, Suzanne; Rosa, Rui (2022): Data from: GABAergic role in the disruption of wild cleaner fish behaviour under high CO2. figshare, https://doi.org/10.6084/m9.figshare.13473156

Documentation:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2022): seacarb: seawater carbonate chemistry with R. R package version 3.3.1. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: -17.533000 * Longitude: 149.833000

Event(s):

Moorea_north_shore * Latitude: -17.533000 * Longitude: 149.833000 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-03-20.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Paula, José Ricardo		Study
2	Species, unique identification	Species UID		Paula, José Ricardo
3	Species, unique identification (URI)	Species UID (URI)		Paula, José Ricardo
4	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		Paula, José Ricardo
5	Identification	ID		Paula, José Ricardo		system
6	Treatment	Treat		Paula, José Ricardo
7	Treatment	Treat		Paula, José Ricardo
8	Number	n	#	Paula, José Ricardo		interactions
9	Duration	Duration	s	Paula, José Ricardo		total interactions
10	Number	n	#	Paula, José Ricardo		interactions started by cleaners
11	Duration	Duration	s	Paula, José Ricardo		performing advertising dance
12	Number	n	#	Paula, José Ricardo		interactions with tactile stimulation
13	Number	n	#	Paula, José Ricardo		interactions with jolts
14	Number	n	#	Paula, José Ricardo		posing_clients
15	Temperature, water	Temp	°C	Paula, José Ricardo
16	Temperature, water, standard deviation	Temp std dev	±	Paula, José Ricardo
17	pH	pH		Paula, José Ricardo	Potentiometric	total scale
18	pH, standard deviation	pH std dev	±	Paula, José Ricardo	Potentiometric	total scale
19	Alkalinity, total	AT	µmol/kg	Paula, José Ricardo	Potentiometric titration
20	Alkalinity, total, standard deviation	AT std dev	±	Paula, José Ricardo	Potentiometric titration
21	Salinity	Sal		Paula, José Ricardo
22	Carbon, inorganic, dissolved	DIC	µmol/kg	Paula, José Ricardo	Calculated using CO2SYS
23	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Paula, José Ricardo	Calculated using CO2SYS
24	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Paula, José Ricardo	Calculated using CO2SYS
25	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Paula, José Ricardo	Calculated using CO2SYS
26	Aragonite saturation state	Omega Arg		Paula, José Ricardo	Calculated using CO2SYS
27	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Paula, José Ricardo	Calculated using CO2SYS
28	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
29	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	Carbon dioxide, standard deviation	CO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
31	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
32	Fugacity of carbon dioxide in seawater, standard deviation	fCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
33	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
35	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
37	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Carbonate ion, standard deviation	[CO3]2- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
39	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
40	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
41	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
43	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Calcite saturation state, standard deviation	Omega Cal std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

2393 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (Study)	2 Species UID	3 Species UID (URI)	4 Species UID (Semantic URI)	5 ID (system)	6 Treat	7 Treat	8 n [#] (interactions)	9 Duration [s] (total interactions)	10 n [#] (interactions started by cleaners)	11 Duration [s] (performing advertising dance)	12 n [#] (interactions with tactile sti...)	13 n [#] (interactions with jolts)	14 n [#] (posing_clients)	15 Temp [°C]	16 Temp std dev [±]	17 pH (total scale, Potentiometric)	18 pH std dev [±] (total scale, Potentiometric)	19 AT [µmol/kg] (Potentiometric titration)	20 AT std dev [±] (Potentiometric titration)	21 Sal	22 DIC [µmol/kg] (Calculated using CO2SYS)	23 DIC std dev [±] (Calculated using CO2SYS)	24 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	25 pCO2 std dev [±] (Calculated using CO2SYS)	26 Omega Arg (Calculated using CO2SYS)	27 Omega Arg std dev [±] (Calculated using CO2SYS)	28 CSC flag (Calculated using seacarb afte...)	29 CO2 [µmol/kg] (Calculated using seacarb afte...)	30 CO2 std dev [±] (Calculated using seacarb afte...)	31 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	32 fCO2 std dev [±] (Calculated using seacarb afte...)	33 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	34 pCO2 std dev [±] (Calculated using seacarb afte...)	35 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	36 [HCO3]- std dev [±] (Calculated using seacarb afte...)	37 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	38 [CO3]2- std dev [±] (Calculated using seacarb afte...)	39 DIC [µmol/kg] (Calculated using seacarb afte...)	40 DIC std dev [±] (Calculated using seacarb afte...)	41 Omega Arg (Calculated using seacarb afte...)	42 Omega Arg std dev [±] (Calculated using seacarb afte...)	43 Omega Cal (Calculated using seacarb afte...)	44 Omega Cal std dev [±] (Calculated using seacarb afte...)
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	c3	Sham	Control	14	72.895	3	118.967	5	0	51	28.7	0.3	8.05	0.01	2428.2	11.8	35	2072.9	12.5	400.8	15	4.1	0.1	8	10.62	0.32	409.38	12.01	410.63	12.05	1815.65	14.91	252.10	4.96	2078.37	12.81	4.08	0.08	6.11	0.12
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	c3	Sham	Control	13	31.901	5	0.000	2	3	38	28.7	0.3	8.05	0.01	2428.2	11.8	35	2072.9	12.5	400.8	15	4.1	0.1	8	10.62	0.32	409.38	12.01	410.63	12.05	1815.65	14.91	252.10	4.96	2078.37	12.81	4.08	0.08	6.11	0.12
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	c2	Sham	Control	3	30.583	1	0.000	3	0	11	28.7	0.3	8.05	0.01	2428.2	11.8	35	2072.9	12.5	400.8	15	4.1	0.1	8	10.62	0.32	409.38	12.01	410.63	12.05	1815.65	14.91	252.10	4.96	2078.37	12.81	4.08	0.08	6.11	0.12
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	c1	Sham	Control	26	242.084	7	79.173	13	1	59	28.7	0.3	8.05	0.01	2428.2	11.8	35	2072.9	12.5	400.8	15	4.1	0.1	8	10.62	0.32	409.38	12.01	410.63	12.05	1815.65	14.91	252.10	4.96	2078.37	12.81	4.08	0.08	6.11	0.12
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	c2	Sham	Control	28	226.363	4	16.555	12	12	68	28.7	0.3	8.05	0.01	2428.2	11.8	35	2072.9	12.5	400.8	15	4.1	0.1	8	10.62	0.32	409.38	12.01	410.63	12.05	1815.65	14.91	252.10	4.96	2078.37	12.81	4.08	0.08	6.11	0.12
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	c1	Sham	Control	5	16.495	3	0.000	0	3	13	28.7	0.3	8.05	0.01	2428.2	11.8	35	2072.9	12.5	400.8	15	4.1	0.1	8	10.62	0.32	409.38	12.01	410.63	12.05	1815.65	14.91	252.10	4.96	2078.37	12.81	4.08	0.08	6.11	0.12
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	c3	Sham	Control	12	56.389	5	36.502	5	4	30	28.7	0.3	8.05	0.01	2428.2	11.8	35	2072.9	12.5	400.8	15	4.1	0.1	8	10.62	0.32	409.38	12.01	410.63	12.05	1815.65	14.91	252.10	4.96	2078.37	12.81	4.08	0.08	6.11	0.12
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	c3	Sham	Control	10	49.387	2	38.983	2	3	33	28.7	0.3	8.05	0.01	2428.2	11.8	35	2072.9	12.5	400.8	15	4.1	0.1	8	10.62	0.32	409.38	12.01	410.63	12.05	1815.65	14.91	252.10	4.96	2078.37	12.81	4.08	0.08	6.11	0.12
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	c2	Sham	Control	48	418.109	17	107.844	26	8	102	28.7	0.3	8.05	0.01	2428.2	11.8	35	2072.9	12.5	400.8	15	4.1	0.1	8	10.62	0.32	409.38	12.01	410.63	12.05	1815.65	14.91	252.10	4.96	2078.37	12.81	4.08	0.08	6.11	0.12
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	c2	Sham	Control	8	57.275	4	0.994	5	4	49	28.7	0.3	8.05	0.01	2428.2	11.8	35	2072.9	12.5	400.8	15	4.1	0.1	8	10.62	0.32	409.38	12.01	410.63	12.05	1815.65	14.91	252.10	4.96	2078.37	12.81	4.08	0.08	6.11	0.12
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	c1	Sham	Control	20	65.500	2	0.000	12	7	133	28.7	0.3	8.05	0.01	2428.2	11.8	35	2072.9	12.5	400.8	15	4.1	0.1	8	10.62	0.32	409.38	12.01	410.63	12.05	1815.65	14.91	252.10	4.96	2078.37	12.81	4.08	0.08	6.11	0.12
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	c1	Gabazine	Control	9	34.411	1	1.474	1	2	50	28.7	0.3	8.05	0.01	2428.2	11.8	35	2072.9	12.5	400.8	15	4.1	0.1	8	10.62	0.32	409.38	12.01	410.63	12.05	1815.65	14.91	252.10	4.96	2078.37	12.81	4.08	0.08	6.11	0.12
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	c1	Gabazine	Control	2	18.909	0	1.308	2	1	7	28.7	0.3	8.05	0.01	2428.2	11.8	35	2072.9	12.5	400.8	15	4.1	0.1	8	10.62	0.32	409.38	12.01	410.63	12.05	1815.65	14.91	252.10	4.96	2078.37	12.81	4.08	0.08	6.11	0.12
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	c2	Gabazine	Control	1	20.753	0	0.000	0	0	19	28.7	0.3	8.05	0.01	2428.2	11.8	35	2072.9	12.5	400.8	15	4.1	0.1	8	10.62	0.32	409.38	12.01	410.63	12.05	1815.65	14.91	252.10	4.96	2078.37	12.81	4.08	0.08	6.11	0.12
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	c1	Gabazine	Control	11	59.936	2	98.373	2	2	16	28.7	0.3	8.05	0.01	2428.2	11.8	35	2072.9	12.5	400.8	15	4.1	0.1	8	10.62	0.32	409.38	12.01	410.63	12.05	1815.65	14.91	252.10	4.96	2078.37	12.81	4.08	0.08	6.11	0.12
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	c3	Gabazine	Control	9	10.291	9	52.649	0	0	12	28.7	0.3	8.05	0.01	2428.2	11.8	35	2072.9	12.5	400.8	15	4.1	0.1	8	10.62	0.32	409.38	12.01	410.63	12.05	1815.65	14.91	252.10	4.96	2078.37	12.81	4.08	0.08	6.11	0.12
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	c2	Gabazine	Control	8	63.953	4	103.668	3	0	14	28.7	0.3	8.05	0.01	2428.2	11.8	35	2072.9	12.5	400.8	15	4.1	0.1	8	10.62	0.32	409.38	12.01	410.63	12.05	1815.65	14.91	252.10	4.96	2078.37	12.81	4.08	0.08	6.11	0.12
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	c2	Gabazine	Control	9	38.207	2	0.000	4	3	45	28.7	0.3	8.05	0.01	2428.2	11.8	35	2072.9	12.5	400.8	15	4.1	0.1	8	10.62	0.32	409.38	12.01	410.63	12.05	1815.65	14.91	252.10	4.96	2078.37	12.81	4.08	0.08	6.11	0.12
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	c3	Gabazine	Control	21	308.262	4	49.138	9	10	38	28.7	0.3	8.05	0.01	2428.2	11.8	35	2072.9	12.5	400.8	15	4.1	0.1	8	10.62	0.32	409.38	12.01	410.63	12.05	1815.65	14.91	252.10	4.96	2078.37	12.81	4.08	0.08	6.11	0.12
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	c3	Gabazine	Control	5	109.717	0	0.000	2	4	17	28.7	0.3	8.05	0.01	2428.2	11.8	35	2072.9	12.5	400.8	15	4.1	0.1	8	10.62	0.32	409.38	12.01	410.63	12.05	1815.65	14.91	252.10	4.96	2078.37	12.81	4.08	0.08	6.11	0.12
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	c3	Gabazine	Control	17	61.299	3	23.868	8	5	55	28.7	0.3	8.05	0.01	2428.2	11.8	35	2072.9	12.5	400.8	15	4.1	0.1	8	10.62	0.32	409.38	12.01	410.63	12.05	1815.65	14.91	252.10	4.96	2078.37	12.81	4.08	0.08	6.11	0.12
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	a2	Sham	High CO2	6	187.938	0	1.320	4	0	25	28.7	0.3	7.70	0.02	2428.2	11.8	35	2263.9	12.5	1029.2	44	2.2	0.1	8	27.65	1.46	1065.69	55.76	1068.95	55.93	2111.23	16.76	130.94	5.42	2269.82	14.52	2.12	0.09	3.17	0.13
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	a1	Sham	High CO2	14	42.584	2	1.494	0	1	23	28.7	0.3	7.70	0.02	2428.2	11.8	35	2263.9	12.5	1029.2	44	2.2	0.1	8	27.65	1.46	1065.69	55.76	1068.95	55.93	2111.23	16.76	130.94	5.42	2269.82	14.52	2.12	0.09	3.17	0.13
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	a1	Sham	High CO2	1	39.262	1	1.137	1	1	18	28.7	0.3	7.70	0.02	2428.2	11.8	35	2263.9	12.5	1029.2	44	2.2	0.1	8	27.65	1.46	1065.69	55.76	1068.95	55.93	2111.23	16.76	130.94	5.42	2269.82	14.52	2.12	0.09	3.17	0.13
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	a2	Sham	High CO2	20	80.766	4	0.000	5	5	100	28.7	0.3	7.70	0.02	2428.2	11.8	35	2263.9	12.5	1029.2	44	2.2	0.1	8	27.65	1.46	1065.69	55.76	1068.95	55.93	2111.23	16.76	130.94	5.42	2269.82	14.52	2.12	0.09	3.17	0.13
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	a1	Sham	High CO2	18	143.646	2	25.192	6	10	37	28.7	0.3	7.70	0.02	2428.2	11.8	35	2263.9	12.5	1029.2	44	2.2	0.1	8	27.65	1.46	1065.69	55.76	1068.95	55.93	2111.23	16.76	130.94	5.42	2269.82	14.52	2.12	0.09	3.17	0.13
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	a1	Sham	High CO2	28	386.281	3	27.963	16	10	127	28.7	0.3	7.70	0.02	2428.2	11.8	35	2263.9	12.5	1029.2	44	2.2	0.1	8	27.65	1.46	1065.69	55.76	1068.95	55.93	2111.23	16.76	130.94	5.42	2269.82	14.52	2.12	0.09	3.17	0.13
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	a3	Sham	High CO2	9	15.861	2	0.000	1	1	84	28.7	0.3	7.70	0.02	2428.2	11.8	35	2263.9	12.5	1029.2	44	2.2	0.1	8	27.65	1.46	1065.69	55.76	1068.95	55.93	2111.23	16.76	130.94	5.42	2269.82	14.52	2.12	0.09	3.17	0.13
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	a2	Sham	High CO2	10	32.077	5	0.000	1	5	27	28.7	0.3	7.70	0.02	2428.2	11.8	35	2263.9	12.5	1029.2	44	2.2	0.1	8	27.65	1.46	1065.69	55.76	1068.95	55.93	2111.23	16.76	130.94	5.42	2269.82	14.52	2.12	0.09	3.17	0.13
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	a3	Sham	High CO2	12	43.941	4	4.254	3	4	38	28.7	0.3	7.70	0.02	2428.2	11.8	35	2263.9	12.5	1029.2	44	2.2	0.1	8	27.65	1.46	1065.69	55.76	1068.95	55.93	2111.23	16.76	130.94	5.42	2269.82	14.52	2.12	0.09	3.17	0.13
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	a3	Sham	High CO2	5	15.028	1	0.000	1	0	24	28.7	0.3	7.70	0.02	2428.2	11.8	35	2263.9	12.5	1029.2	44	2.2	0.1	8	27.65	1.46	1065.69	55.76	1068.95	55.93	2111.23	16.76	130.94	5.42	2269.82	14.52	2.12	0.09	3.17	0.13
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	a2	Gabazine	High CO2	23	156.817	4	0.000	13	5	71	28.7	0.3	7.70	0.02	2428.2	11.8	35	2263.9	12.5	1029.2	44	2.2	0.1	8	27.65	1.46	1065.69	55.76	1068.95	55.93	2111.23	16.76	130.94	5.42	2269.82	14.52	2.12	0.09	3.17	0.13
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	a1	Gabazine	High CO2	17	75.205	6	0.000	5	6	39	28.7	0.3	7.70	0.02	2428.2	11.8	35	2263.9	12.5	1029.2	44	2.2	0.1	8	27.65	1.46	1065.69	55.76	1068.95	55.93	2111.23	16.76	130.94	5.42	2269.82	14.52	2.12	0.09	3.17	0.13
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	a1	Gabazine	High CO2	20	138.024	3	1.277	8	3	70	28.7	0.3	7.70	0.02	2428.2	11.8	35	2263.9	12.5	1029.2	44	2.2	0.1	8	27.65	1.46	1065.69	55.76	1068.95	55.93	2111.23	16.76	130.94	5.42	2269.82	14.52	2.12	0.09	3.17	0.13
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	a3	Gabazine	High CO2	21	112.766	3	0.000	8	8	104	28.7	0.3	7.70	0.02	2428.2	11.8	35	2263.9	12.5	1029.2	44	2.2	0.1	8	27.65	1.46	1065.69	55.76	1068.95	55.93	2111.23	16.76	130.94	5.42	2269.82	14.52	2.12	0.09	3.17	0.13
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	a1	Gabazine	High CO2	17	182.984	3	14.193	11	6	53	28.7	0.3	7.70	0.02	2428.2	11.8	35	2263.9	12.5	1029.2	44	2.2	0.1	8	27.65	1.46	1065.69	55.76	1068.95	55.93	2111.23	16.76	130.94	5.42	2269.82	14.52	2.12	0.09	3.17	0.13
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	a3	Gabazine	High CO2	57	543.598	1	22.597	48	14	104	28.7	0.3	7.70	0.02	2428.2	11.8	35	2263.9	12.5	1029.2	44	2.2	0.1	8	27.65	1.46	1065.69	55.76	1068.95	55.93	2111.23	16.76	130.94	5.42	2269.82	14.52	2.12	0.09	3.17	0.13
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	a3	Gabazine	High CO2	36	225.923	3	9.298	16	6	125	28.7	0.3	7.70	0.02	2428.2	11.8	35	2263.9	12.5	1029.2	44	2.2	0.1	8	27.65	1.46	1065.69	55.76	1068.95	55.93	2111.23	16.76	130.94	5.42	2269.82	14.52	2.12	0.09	3.17	0.13
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	a3	Gabazine	High CO2	35	521.288	1	41.844	29	18	77	28.7	0.3	7.70	0.02	2428.2	11.8	35	2263.9	12.5	1029.2	44	2.2	0.1	8	27.65	1.46	1065.69	55.76	1068.95	55.93	2111.23	16.76	130.94	5.42	2269.82	14.52	2.12	0.09	3.17	0.13
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	a3	Muscimol	High CO2	12	53.810	3	0.000	2	4	67	28.7	0.3	7.70	0.02	2428.2	11.8	35	2263.9	12.5	1029.2	44	2.2	0.1	8	27.65	1.46	1065.69	55.76	1068.95	55.93	2111.23	16.76	130.94	5.42	2269.82	14.52	2.12	0.09	3.17	0.13
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	a2	Muscimol	High CO2	8	23.624	1	1.506	4	5	49	28.7	0.3	7.70	0.02	2428.2	11.8	35	2263.9	12.5	1029.2	44	2.2	0.1	8	27.65	1.46	1065.69	55.76	1068.95	55.93	2111.23	16.76	130.94	5.42	2269.82	14.52	2.12	0.09	3.17	0.13
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	a3	Muscimol	High CO2	25	103.913	3	0.000	7	6	59	28.7	0.3	7.70	0.02	2428.2	11.8	35	2263.9	12.5	1029.2	44	2.2	0.1	8	27.65	1.46	1065.69	55.76	1068.95	55.93	2111.23	16.76	130.94	5.42	2269.82	14.52	2.12	0.09	3.17	0.13
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	a1	Muscimol	High CO2	2	1.358	2	1.498	0	2	12	28.7	0.3	7.70	0.02	2428.2	11.8	35	2263.9	12.5	1029.2	44	2.2	0.1	8	27.65	1.46	1065.69	55.76	1068.95	55.93	2111.23	16.76	130.94	5.42	2269.82	14.52	2.12	0.09	3.17	0.13
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	a3	Muscimol	High CO2	24	149.931	11	3.747	11	9	68	28.7	0.3	7.70	0.02	2428.2	11.8	35	2263.9	12.5	1029.2	44	2.2	0.1	8	27.65	1.46	1065.69	55.76	1068.95	55.93	2111.23	16.76	130.94	5.42	2269.82	14.52	2.12	0.09	3.17	0.13
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	a3	Muscimol	High CO2	2	12.260	2		0	0	2	28.7	0.3	7.70	0.02	2428.2	11.8	35	2263.9	12.5	1029.2	44	2.2	0.1	8	27.65	1.46	1065.69	55.76	1068.95	55.93	2111.23	16.76	130.94	5.42	2269.82	14.52	2.12	0.09	3.17	0.13
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	a2	Muscimol	High CO2	23	460.187	10	76.950	18	11	55	28.7	0.3	7.70	0.02	2428.2	11.8	35	2263.9	12.5	1029.2	44	2.2	0.1	8	27.65	1.46	1065.69	55.76	1068.95	55.93	2111.23	16.76	130.94	5.42	2269.82	14.52	2.12	0.09	3.17	0.13
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	a1	Muscimol	High CO2	41	198.159	3	0.000	10	9	82	28.7	0.3	7.70	0.02	2428.2	11.8	35	2263.9	12.5	1029.2	44	2.2	0.1	8	27.65	1.46	1065.69	55.76	1068.95	55.93	2111.23	16.76	130.94	5.42	2269.82	14.52	2.12	0.09	3.17	0.13
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	a2	Muscimol	High CO2	34	259.840	7	0.000	13	2	97	28.7	0.3	7.70	0.02	2428.2	11.8	35	2263.9	12.5	1029.2	44	2.2	0.1	8	27.65	1.46	1065.69	55.76	1068.95	55.93	2111.23	16.76	130.94	5.42	2269.82	14.52	2.12	0.09	3.17	0.13
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	a2	Muscimol	High CO2	13	24.057	4	1.249	1	10	77	28.7	0.3	7.70	0.02	2428.2	11.8	35	2263.9	12.5	1029.2	44	2.2	0.1	8	27.65	1.46	1065.69	55.76	1068.95	55.93	2111.23	16.76	130.94	5.42	2269.82	14.52	2.12	0.09	3.17	0.13
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	c3	Muscimol	Control	12	32.862	2	0.000	5	8	42	28.7	0.3	8.05	0.01	2428.2	11.8	35	2072.9	12.5	400.8	15	4.1	0.1	8	10.62	0.32	409.38	12.01	410.63	12.05	1815.65	14.91	252.10	4.96	2078.37	12.81	4.08	0.08	6.11	0.12
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	c1	Muscimol	Control	0	0.000	0	2.176	0	0	24	28.7	0.3	8.05	0.01	2428.2	11.8	35	2072.9	12.5	400.8	15	4.1	0.1	8	10.62	0.32	409.38	12.01	410.63	12.05	1815.65	14.91	252.10	4.96	2078.37	12.81	4.08	0.08	6.11	0.12
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	c1	Muscimol	Control	5	8.803	2	1.260	0	1	21	28.7	0.3	8.05	0.01	2428.2	11.8	35	2072.9	12.5	400.8	15	4.1	0.1	8	10.62	0.32	409.38	12.01	410.63	12.05	1815.65	14.91	252.10	4.96	2078.37	12.81	4.08	0.08	6.11	0.12
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	c1	Muscimol	Control	11	28.370	0	5.000	2	2	38	28.7	0.3	8.05	0.01	2428.2	11.8	35	2072.9	12.5	400.8	15	4.1	0.1	8	10.62	0.32	409.38	12.01	410.63	12.05	1815.65	14.91	252.10	4.96	2078.37	12.81	4.08	0.08	6.11	0.12
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	c3	Muscimol	Control	12	72.511	4	0.000	5	1	52	28.7	0.3	8.05	0.01	2428.2	11.8	35	2072.9	12.5	400.8	15	4.1	0.1	8	10.62	0.32	409.38	12.01	410.63	12.05	1815.65	14.91	252.10	4.96	2078.37	12.81	4.08	0.08	6.11	0.12
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	c2	Muscimol	Control	19	66.331	10	10.979	4	9	75	28.7	0.3	8.05	0.01	2428.2	11.8	35	2072.9	12.5	400.8	15	4.1	0.1	8	10.62	0.32	409.38	12.01	410.63	12.05	1815.65	14.91	252.10	4.96	2078.37	12.81	4.08	0.08	6.11	0.12
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	c1	Muscimol	Control	3	10.359	0	25.460	2	0	29	28.7	0.3	8.05	0.01	2428.2	11.8	35	2072.9	12.5	400.8	15	4.1	0.1	8	10.62	0.32	409.38	12.01	410.63	12.05	1815.65	14.91	252.10	4.96	2078.37	12.81	4.08	0.08	6.11	0.12
laboratory	Labroides dimidiatus	marinespecies.org	urn:lsid:marinespecies.org:taxname:219014	c1	Muscimol	Control	79	465.601	9	24.287	39	22	142	28.7	0.3	8.05	0.01	2428.2	11.8	35	2072.9	12.5	400.8	15	4.1	0.1	8	10.62	0.32	409.38	12.01	410.63	12.05	1815.65	14.91	252.10	4.96	2078.37	12.81	4.08	0.08	6.11	0.12