Mincarelli Luana Fiorella 0000-0001-5436-7738 Chapman Emma C Rotchell Jeanette M Turner Alexander P Wollenberg Valero Katharina C k.wollenberg-valero@hull.ac.uk 0000-0001-8858-1804 2022 Seawater carbonate chemistry and gene expression in Mytilus edulis dataset https://doi.org/10.1594/PANGAEA.956746 Plastic pollution and changes in oceanic pH are both pressing environmental issues. Little emphasis, however, has been placed on the influence of sex and gametogenesis stage when investigating the effects of such stressors. Here, we examined histology and molecular biomarkers of blue mussels Mytilus edulis exposed for 7 days to a pH 7.7 scenario (− 0.4 units) in combination with environmentally relevant concentrations (0, 0.5 and 50 µg/L) of the endocrine disrupting plasticiser di-2-ethylhexyl phthalate (DEHP). Through a factorial design, we investigated the gametogenesis cycle and sex-related expression of genes involved in pH homeostasis, stress response and oestrogen receptor-like pathways after the exposure to the two environmental stressors. As expected, we found sex-related differences in the proportion of developing, mature and spawning gonads in histological sections. Male gonads also showed higher levels of the acid–base regulator CA2, but females had a higher expression of stress response-related genes (i.e. sod, cat, hsp70). We found a significant effect of DEHP on stress response-related gene expression that was dependent on the gametogenesis stage, but there was only a trend towards downregulation of CA2 in response to pH 7.7. In addition, differences in gene expression between males and females were most pronounced in experimental conditions containing DEHP and/or acidified pH but never the control, indicating that it is important to consider sex and gametogenesis stage when studying the response of mussels to diverse stressors. Mincarelli Luana Fiorella 0000-0001-5436-7738 Chapman Emma C Rotchell Jeanette M Turner Alexander P Wollenberg Valero Katharina C k.wollenberg-valero@hull.ac.uk 0000-0001-8858-1804 2022 Sex and gametogenesis stage are strong drivers of gene expression in Mytilus edulis exposed to environmentally relevant plasticiser levels and pH 7.7 journal article Environmental Science and Pollution Research 30(9) https://doi.org/10.1007/s11356-022-23801-3 23437-23449 Gattuso Jean-Pierre jean-pierre.gattuso@imev-mer.fr 0000-0002-4533-4114 Epitalon Jean-Marie Lavigne Héloïse hlavigne@naturalsciences.be Orr James James.Orr@lsce.ipsl.fr Gentili Bernard bernard.gentili@orange.fr http://omtab.obs-vlfr.fr/personnes/z_gent_be/gentili.php Hagens Mathilde 0000-0003-3980-1043 Hofmann Andreas Mueller Jens-Daniel Proye Aurélien Rae James 0000-0003-3904-2526 Soetaert Karline karline.soetaert@nioz.nl 0000-0003-4603-7100 2022 seacarb: seawater carbonate chemistry with R. R package version 3.3.1 other https://cran.r-project.org/web/packages/seacarb/index.html not specified OA-ICC Ocean Acidification International Coordination Centre https://www.iaea.org/services/oa-icc/science-and-collaboration/data-access-and-management International Atomic Energy Agency IAEA http://www.iaea.org https://ror.org/02zt1gg83 10995 CC-BY-4.0 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-03-13. Type Type type Wollenberg Valero Katharina C k.wollenberg-valero@hull.ac.uk 0000-0001-8858-1804 Study Type (Study) Species, unique identification Species UID species Wollenberg Valero Katharina C k.wollenberg-valero@hull.ac.uk 0000-0001-8858-1804 Species UID Species, unique identification (URI) Species UID (URI) species Wollenberg Valero Katharina C k.wollenberg-valero@hull.ac.uk 0000-0001-8858-1804 Species UID (URI) Species, unique identification (Semantic URI) Species UID (Semantic URI) species Wollenberg Valero Katharina C k.wollenberg-valero@hull.ac.uk 0000-0001-8858-1804 Species UID (Semantic URI) Figure Fig Wollenberg Valero Katharina C k.wollenberg-valero@hull.ac.uk 0000-0001-8858-1804 Fig Treatment Treat treatment Wollenberg Valero Katharina C k.wollenberg-valero@hull.ac.uk 0000-0001-8858-1804 pH Treat (pH) Treatment Treat treatment Wollenberg Valero Katharina C k.wollenberg-valero@hull.ac.uk 0000-0001-8858-1804 Concentrations of DEHP Treat (Concentrations of DEHP) Sex Sex sex Wollenberg Valero Katharina C k.wollenberg-valero@hull.ac.uk 0000-0001-8858-1804 Sex Stage Stage Wollenberg Valero Katharina C k.wollenberg-valero@hull.ac.uk 0000-0001-8858-1804 Stage Gene expression Gene expression gene expression Wollenberg Valero Katharina C k.wollenberg-valero@hull.ac.uk 0000-0001-8858-1804 sod Gene expression (sod) Gene expression Gene expression gene expression Wollenberg Valero Katharina C k.wollenberg-valero@hull.ac.uk 0000-0001-8858-1804 cat Gene expression (cat) Gene expression Gene expression gene expression Wollenberg Valero Katharina C k.wollenberg-valero@hull.ac.uk 0000-0001-8858-1804 hsp70 Gene expression (hsp70) Gene expression Gene expression gene expression Wollenberg Valero Katharina C k.wollenberg-valero@hull.ac.uk 0000-0001-8858-1804 CA2 Gene expression (CA2) Gene expression Gene expression gene expression Wollenberg Valero Katharina C k.wollenberg-valero@hull.ac.uk 0000-0001-8858-1804 MeER1 Gene expression (MeER1) Gene expression Gene expression gene expression Wollenberg Valero Katharina C k.wollenberg-valero@hull.ac.uk 0000-0001-8858-1804 MeER2 Gene expression (MeER2) Salinity Sal salinity http://purl.obolibrary.org/obo/PATO_0085001 Wollenberg Valero Katharina C k.wollenberg-valero@hull.ac.uk 0000-0001-8858-1804 Sal Salinity, standard deviation Sal std dev ± standard deviation std dev https://en.wikipedia.org/wiki/Standard_deviation Wollenberg Valero Katharina C k.wollenberg-valero@hull.ac.uk 0000-0001-8858-1804 Sal std dev [±] Temperature, water Temp °C Temperature Θ http://dbpedia.org/resource/Temperature water http://purl.obolibrary.org/obo/CHEBI_15377 Wollenberg Valero Katharina C k.wollenberg-valero@hull.ac.uk 0000-0001-8858-1804 Temp [°C] Temperature, water, standard deviation Temp std dev ± Temperature Θ http://dbpedia.org/resource/Temperature water http://purl.obolibrary.org/obo/CHEBI_15377 standard deviation std dev https://en.wikipedia.org/wiki/Standard_deviation Wollenberg Valero Katharina C k.wollenberg-valero@hull.ac.uk 0000-0001-8858-1804 Temp std dev [±] pH, NBS scale pH NBS Wollenberg Valero Katharina C k.wollenberg-valero@hull.ac.uk 0000-0001-8858-1804 NBS scale pH NBS (NBS scale) pH, standard deviation pH std dev ± standard deviation std dev https://en.wikipedia.org/wiki/Standard_deviation Wollenberg Valero Katharina C k.wollenberg-valero@hull.ac.uk 0000-0001-8858-1804 NBS scale pH std dev [±] (NBS scale) Alkalinity, total AT mg/l total https://www.oxforddictionaries.com/definition/american_english/total Density http://dbpedia.org/resource/Density Wollenberg Valero Katharina C k.wollenberg-valero@hull.ac.uk 0000-0001-8858-1804 AT [mg/l] Alkalinity, total, standard deviation AT std dev ± total https://www.oxforddictionaries.com/definition/american_english/total standard deviation std dev https://en.wikipedia.org/wiki/Standard_deviation Wollenberg Valero Katharina C k.wollenberg-valero@hull.ac.uk 0000-0001-8858-1804 AT std dev [±] Alkalinity, total AT µmol/kg total https://www.oxforddictionaries.com/definition/american_english/total Molar Concentration Wollenberg Valero Katharina C k.wollenberg-valero@hull.ac.uk 0000-0001-8858-1804 AT [µmol/kg] Carbonate system computation flag CSC flag Calculated using seacarb after Nisumaa et al. (2010) https://doi.org/10.5194/essd-2-167-2010 Yang Yan yangyan@xmu.edu.cn http://mel.xmu.edu.cn/en/ 0000-0003-4900-5606 CSC flag pH, total scale pHT Calculated using seacarb after Nisumaa et al. (2010) https://doi.org/10.5194/essd-2-167-2010 Yang Yan yangyan@xmu.edu.cn http://mel.xmu.edu.cn/en/ 0000-0003-4900-5606 total scale pHT (total scale) Carbon dioxide CO2 µmol/kg carbon dioxide http://purl.obolibrary.org/obo/CHEBI_16526 Molar Concentration Calculated using seacarb after Nisumaa et al. (2010) https://doi.org/10.5194/essd-2-167-2010 Yang Yan yangyan@xmu.edu.cn http://mel.xmu.edu.cn/en/ 0000-0003-4900-5606 CO2 [µmol/kg] Fugacity of carbon dioxide (water) at sea surface temperature (wet air) fCO2water_SST_wet µatm fugacity https://en.wikipedia.org/wiki/Fugacity carbon dioxide http://purl.obolibrary.org/obo/CHEBI_16526 water http://purl.obolibrary.org/obo/CHEBI_15377 Energy Density http://dbpedia.org/resource/Energy_density Force Per Area Calculated using seacarb after Nisumaa et al. (2010) https://doi.org/10.5194/essd-2-167-2010 Yang Yan yangyan@xmu.edu.cn http://mel.xmu.edu.cn/en/ 0000-0003-4900-5606 fCO2water_SST_wet [µatm] Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) pCO2water_SST_wet µatm partial pressure carbon dioxide http://purl.obolibrary.org/obo/CHEBI_16526 water http://purl.obolibrary.org/obo/CHEBI_15377 Energy Density http://dbpedia.org/resource/Energy_density Force Per Area Calculated using seacarb after Nisumaa et al. (2010) https://doi.org/10.5194/essd-2-167-2010 Yang Yan yangyan@xmu.edu.cn http://mel.xmu.edu.cn/en/ 0000-0003-4900-5606 pCO2water_SST_wet [µatm] Bicarbonate ion [HCO3]- µmol/kg Molar Concentration Calculated using seacarb after Nisumaa et al. (2010) https://doi.org/10.5194/essd-2-167-2010 Yang Yan yangyan@xmu.edu.cn http://mel.xmu.edu.cn/en/ 0000-0003-4900-5606 [HCO3]- [µmol/kg] Carbonate ion [CO3]2- µmol/kg Molar Concentration Calculated using seacarb after Nisumaa et al. (2010) https://doi.org/10.5194/essd-2-167-2010 Yang Yan yangyan@xmu.edu.cn http://mel.xmu.edu.cn/en/ 0000-0003-4900-5606 [CO3]2- [µmol/kg] Carbon, inorganic, dissolved DIC µmol/kg carbon C https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:27594 dissolved http://purl.obolibrary.org/obo/PATO_0001986 Molar Concentration Calculated using seacarb after Nisumaa et al. (2010) https://doi.org/10.5194/essd-2-167-2010 Yang Yan yangyan@xmu.edu.cn http://mel.xmu.edu.cn/en/ 0000-0003-4900-5606 DIC [µmol/kg] Aragonite saturation state Omega Arg aragonite saturation state Calculated using seacarb after Nisumaa et al. (2010) https://doi.org/10.5194/essd-2-167-2010 Yang Yan yangyan@xmu.edu.cn http://mel.xmu.edu.cn/en/ 0000-0003-4900-5606 Omega Arg Calcite saturation state Omega Cal Calculated using seacarb after Nisumaa et al. (2010) https://doi.org/10.5194/essd-2-167-2010 Yang Yan yangyan@xmu.edu.cn http://mel.xmu.edu.cn/en/ 0000-0003-4900-5606 Omega Cal CurationLevelC Enhanced curation https://wiki.pangaea.de/wiki/Curation_levels citable Animalia Benthic animals Benthos Bottles or small containers/Aquaria (<20 L) Gene expression (incl. proteomics) Laboratory experiment Laboratory strains Mollusca Mytilus edulis Not applicable Ocean acidification Organic toxins Single species author22144 author22159 author27217 author34379 author39204 author51658 author56746 author59171 author59172 author59173 author60641 author69140 author70484 author98425 author98426 author98427 curlevel30 inst20253 journal5068 license21 method6426 param106521 param125247 param132314 param21197 param26954 param28117 param48494 param49312 param49313 param50001 param510679 param534368 param534369 param54293 param54294 param716 param717 param80114 param82111 param82364 param82365 param82366 param87073 param87459 param91410 param96278 pi48750 pi70484 project4173 ref117529 ref117630 term1073490 term1073493 term2606205 term2698012 term37764 term38253 term38477 term38841 term38844 term38857 term38860 term38869 term38874 term38878 term38895 term38900 term39228 term39425 term40646 term41013 term41845 term43298 term43792 term43821 term43834 term43972 term43995 term44030 term44373 term48166 term49384 term49506 term73928 topotype1