PANGAEA_956746 Seawater carbonate chemistry and gene expression in Mytilus edulis Mincarelli, Luana Fiorella; Chapman, Emma C; Rotchell, Jeanette M; Turner, Alexander P; Wollenberg Valero, Katharina C Seawater carbonate chemistry and gene expression in Mytilus edulis 2022 PANGAEA Dataset https://doi.pangaea.de/10.1594/PANGAEA.956746 Investigator Katharina C Wollenberg Valero k.wollenberg-valero@hull.ac.uk Investigator Yan Yang yangyan@xmu.edu.cn Earth Science Type Species, unique identification Species, unique identification (URI) Species, unique identification (Semantic URI) Figure Treatment Sex Stage Gene expression Salinity Salinity, standard deviation Temperature, water Temperature, water, standard deviation pH, NBS scale pH, standard deviation Alkalinity, total Alkalinity, total, standard deviation Alkalinity, total Carbonate system computation flag pH, total scale Carbon dioxide Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Bicarbonate ion Carbonate ion Carbon, inorganic, dissolved Aragonite saturation state Calcite saturation state geoscientificInformation Animalia Benthic animals Benthos Bottles or small containers/Aquaria (<20 L) Gene expression (incl. proteomics) Laboratory experiment Laboratory strains Mollusca Mytilus edulis Not applicable Ocean acidification Organic toxins Single species Calculated using seacarb after Nisumaa et al. (2010) Complete OA-ICC Ocean Acidification International Coordination Centre unrestricted CC-BY-4.0: Creative Commons Attribution 4.0 International English PANGAEA Data Publisher for Earth & Environmental Science https://www.pangaea.de/ Data Center Contact Michael Diepenbroek info@pangaea.de

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Bremen Bremen 28359 Germany online 10995 data points text/tab-separated-values Mincarelli, Luana Fiorella; Chapman, Emma C; Rotchell, Jeanette M; Turner, Alexander P; Wollenberg Valero, Katharina C (2022): Sex and gametogenesis stage are strong drivers of gene expression in Mytilus edulis exposed to environmentally relevant plasticiser levels and pH 7.7. Environmental Science and Pollution Research, 30(9), 23437-23449, https://doi.org/10.1007/s11356-022-23801-3 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2022): seacarb: seawater carbonate chemistry with R. R package version 3.3.1. https://cran.r-project.org/web/packages/seacarb/index.html

Plastic pollution and changes in oceanic pH are both pressing environmental issues. Little emphasis, however, has been placed on the influence of sex and gametogenesis stage when investigating the effects of such stressors. Here, we examined histology and molecular biomarkers of blue mussels Mytilus edulis exposed for 7 days to a pH 7.7 scenario (− 0.4 units) in combination with environmentally relevant concentrations (0, 0.5 and 50 µg/L) of the endocrine disrupting plasticiser di-2-ethylhexyl phthalate (DEHP). Through a factorial design, we investigated the gametogenesis cycle and sex-related expression of genes involved in pH homeostasis, stress response and oestrogen receptor-like pathways after the exposure to the two environmental stressors. As expected, we found sex-related differences in the proportion of developing, mature and spawning gonads in histological sections. Male gonads also showed higher levels of the acid–base regulator CA2, but females had a higher expression of stress response-related genes (i.e. sod, cat, hsp70). We found a significant effect of DEHP on stress response-related gene expression that was dependent on the gametogenesis stage, but there was only a trend towards downregulation of CA2 in response to pH 7.7. In addition, differences in gene expression between males and females were most pronounced in experimental conditions containing DEHP and/or acidified pH but never the control, indicating that it is important to consider sex and gametogenesis stage when studying the response of mussels to diverse stressors. ** For all details see the full metadata description at "https://doi.pangaea.de/10.1594/PANGAEA.956746"! ** In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-03-13.

http://mel.xmu.edu.cn/en/ https://cran.r-project.org/web/packages/seacarb/index.html seacarb: seawater carbonate chemistry with R. R package version 3.3.1 https://doi.org/10.1007/s11356-022-23801-3 Sex and gametogenesis stage are strong drivers of gene expression in Mytilus edulis exposed to environmentally relevant plasticiser levels and pH 7.7 https://doi.org/10.5194/essd-2-167-2010 Calculated using seacarb after Nisumaa et al. (2010) https://www.iaea.org/services/oa-icc/science-and-collaboration/data-access-and-management OA-ICC DIF 9.4 2026-05-19