Citation:

PANGAEA.956053

Name: Prokaryotic variables from a indoor-mesocosm experiment measured during the winter in the Northern Bothnian Sea
Published: 2023-02-28
License: https://creativecommons.org/licenses/by/4.0/
Keywords: 16S metabarcoding; growth rate; mesocosm experiment; prokaryote; prokaryotic abundance; Respiration

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Published: 2023-02-28 • DOI registered: 2023-03-29

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Abstract:

The primary data was collected during the indoor-mesocosm experiment conducted in March 2020 at Umea Marine Science Centre, Umea University, Sweden situated in the Northern Bothnian Sea (63° 34ˈN, 19° 50ˈE). A full factorial experiment was set with temperature and the addition of nutrients as treatment factors with a natural pelagic food web containing all trophic levels except fish. A total of four experimental treatments were set up with three replicates each: C, control (1°C, no additions); N (1°C,+ nutrients); T (10°C, no additions) and TN (10°C, + nutrients). For each treatment, eight different samplings were done in triplicates. The variables in the data include the prokaryotic abundance (PA), growth (PG), respiration (PR), specific prokaryotic respiration (ρ), specific growth rates (µ), growth efficiency (PGE), dissolved organic carbon (DOC), total dissolved phosphorus (TDP) and total dissolved nitrogen (TDN).

Keyword(s):

16S metabarcoding; growth rate; mesocosm experiment; prokaryote; prokaryotic abundance; Respiration

Supplement to:

Verma, Ashish; Amnebrink, Dennis; Pinhassi, Jarone; Wikner, Johan (2022): Prokaryotic maintenance respiration and growth efficiency field patterns reproduced by temperature and nutrient control at mesocosm scale. Environmental Microbiology, 1462-2920.16300, https://doi.org/10.1111/1462-2920.16300

Funding:

Kempe Foundation, grant/award no. SMK-185: Prokaryotic maintenance respiration

Coverage:

Latitude: 63.566667 * Longitude: 19.833333

Date/Time Start: 2020-03-06T00:00:00 * Date/Time End: 2020-04-03T00:00:00

Event(s):

UMU_Mesocosm_2020 * Latitude: 63.566667 * Longitude: 19.833333 * Date/Time Start: 2020-03-06T00:00:00 * Date/Time End: 2020-04-03T00:00:00 * Method/Device: Mesocosm experiment (MESO)

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Experiment day	Exp day	day	Verma, Ashish
2	Sampling	Sampling		Verma, Ashish
3	Mesocosm label	Meso label		Verma, Ashish		name
4	Mesocosm label	Meso label		Verma, Ashish		code
5	Experimental treatment	Exp treat		Verma, Ashish
6	Replicate	Repl		Verma, Ashish
7	Prokaryotes	Prokaryotes	#/l	Verma, Ashish	Epifluorescence microscopy	PA
8	Prokaryotes, production of cells	Prok prod cell	#/l/day	Verma, Ashish	Thymidine incorporation	PG
9	Respiration rate, oxygen, prokaryotic	Prok resp O2	µmol/l/day	Verma, Ashish	Oxygen optode (OPTODE)	PR
10	Respiration rate, oxygen, prokaryotic, per cell	Prok resp O2/cell	fmol/#/day	Verma, Ashish	Calculated from respiration rate and abundance	=PR/PA
11	Specific growth rate	SGR	%/day	Verma, Ashish		=PG/PA
12	Prokaryotes, growth efficiency	PGE		Verma, Ashish	Calculated from growth rate and respiration rate	=PG/PR+PG
13	Carbon, organic, dissolved	DOC	µmol/l	Verma, Ashish	QuAAtro AutoAnalyzer
14	Phosphorus, total dissolved	TDP	µmol/l	Verma, Ashish	QuAAtro AutoAnalyzer
15	Nitrogen, total dissolved	TDN	µmol/l	Verma, Ashish	Shimadzu Total Organic Carbon Analyzer (TOC-5000A)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1440 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Exp day [day]	2 Sampling	3 Meso label (name)	4 Meso label (code)	5 Exp treat	6 Repl	7 Prokaryotes [#/l] (PA, Epifluorescence microscopy)	8 Prok prod cell [#/l/day] (PG, Thymidine incorporation)	9 Prok resp O2 [µmol/l/day] (PR, Oxygen optode)	10 Prok resp O2/cell [fmol/#/day] (=PR/PA, Calculated from respi...)	11 SGR [%/day] (=PG/PA)	12 PGE (=PG/PR+PG, Calculated from gr...)	13 DOC [µmol/l] (QuAAtro AutoAnalyzer)	14 TDP [µmol/l] (QuAAtro AutoAnalyzer)	15 TDN [µmol/l] (Shimadzu Total Organic Carbon...)
3	1	Control	C1	C	1	9.44E8	1.34E8	1.49	1.58	0.140	0.08	352.6	0.290	19.070
3	1	Control	C2	C	2	8.07E8	1.61E8	0.89	1.10	0.200	0.16	349.1	0.300	18.420
3	1	Control	C3	C	3	20.3E8	73.2E6	0.67	0.33	0.040	0.10	355.9	0.300	19.710
6	2	Control	C1	C	1	10.9E8	1.76E8	1.85	1.69	0.160	0.09	359.8	0.250	16.070
6	2	Control	C2	C	2	11.4E8	1.84E8	2.98	2.61	0.160	0.06	362.1	0.270	18.240
6	2	Control	C3	C	3	9.85E8	2.17E8	2.35	2.39	0.220	0.08	372.5	0.280	18.810
10	3	Control	C1	C	1	10.2E8	1.90E8	2.86	2.79	0.190	0.06	362.8	0.250	18.540
10	3	Control	C2	C	2	11.9E8	1.31E8	1.01	0.85	0.110	0.12	407.2	0.260	18.570
10	3	Control	C3	C	3	11.5E8	2.02E8	0.65	0.56	0.180	0.24	385.0	0.260	18.460
13	4	Control	C1	C	1	10.8E8	2.80E8	1.13	1.04	0.260	0.20	368.7	0.250	18.070
13	4	Control	C2	C	2	10.5E8	2.88E8	2.66	2.53	0.270	0.10	352.2	0.220	18.590
13	4	Control	C3	C	3	12.0E8	1.59E8	1.70	1.42	0.130	0.09	351.7	0.230	18.580
17	5	Control	C1	C	1	12.5E8	1.66E8	1.44	1.15	0.130	0.10	341.6	0.220	18.340
17	5	Control	C2	C	2	12.1E8	1.33E8	4.20	3.47	0.110	0.03	355.0	0.210	18.400
17	5	Control	C3	C	3	12.0E8	1.56E8	2.74	2.27	0.130	0.06	356.8	0.220	18.280
20	6	Control	C1	C	1	9.90E8	2.33E8	1.13	1.14	0.240	0.19	380.5	0.160	18.170
20	6	Control	C2	C	2	11.7E8	2.16E8	1.13	0.96	0.180	0.17	373.5	0.140	17.200
20	6	Control	C3	C	3	11.3E8	2.42E8	1.30	1.15	0.210	0.16	368.4	0.160	17.650
24	7	Control	C1	C	1	12.3E8	3.01E8	2.04	1.66	0.240	0.14	368.4	0.110	16.820
24	7	Control	C2	C	2	11.2E8	2.40E8	3.29	2.95	0.220	0.07	367.4	0.120	17.180
24	7	Control	C3	C	3	11.5E8	2.54E8	0.91	0.79	0.220	0.25	357.7	0.150	17.300
27	8	Control	C1	C	1	10.4E8	3.47E8	2.09	2.01	0.330	0.17	356.5	0.100	17.110
27	8	Control	C2	C	2	10.4E8	2.88E8	3.17	3.05	0.280	0.10	356.4	0.100	16.060
27	8	Control	C3	C	3	7.99E8	2.86E8	3.46	4.33	0.360	0.10	357.3	0.100	16.940
3	1	Plus Nutrients	N1	N	1	6.82E8	78.7E6	1.68	2.47	0.120	0.04	367.4	0.410	20.300
3	1	Plus Nutrients	N2	N	2	21.0E8	99.7E6	1.68	0.80	0.050	0.05	357.7	0.410	20.200
3	1	Plus Nutrients	N3	N	3	18.8E8	60.0E6	1.22	0.65	0.030	0.05	355.8	0.390	20.090
6	2	Plus Nutrients	N1	N	1	10.3E8	4.74E8	3.05	2.95	0.460	0.16	356.5	0.380	20.510
6	2	Plus Nutrients	N2	N	2	8.16E8	2.98E8	1.44	1.76	0.360	0.18	356.4	0.430	20.900
6	2	Plus Nutrients	N3	N	3	6.24E8	4.10E8	3.29	5.27	0.660	0.13	357.3	0.440	21.340
10	3	Plus Nutrients	N1	N	1	14.6E8	11.4E8	2.98	2.04	0.780	0.32	368.9	0.350	20.840
10	3	Plus Nutrients	N2	N	2	11.8E8	6.07E8	2.23	1.90	0.520	0.24	361.5	0.340	20.950
10	3	Plus Nutrients	N3	N	3	11.9E8	9.39E8	3.17	2.65	0.790	0.26	363.4	0.340	20.990
13	4	Plus Nutrients	N1	N	1	12.2E8	14.6E8	2.02	1.65	1.190	0.47	349.1	0.390	21.880
13	4	Plus Nutrients	N2	N	2	12.3E8	15.1E8	2.76	2.25	1.230	0.39	353.8	0.390	22.240
13	4	Plus Nutrients	N3	N	3	10.0E8	8.15E8	2.26	2.25	0.810	0.30	365.5	0.400	22.740
17	5	Plus Nutrients	N1	N	1	7.00E8	3.72E8	1.03	1.47	0.530	0.24	361.1	0.430	23.230
17	5	Plus Nutrients	N2	N	2	8.04E8	4.91E8	2.42	3.01	0.610	0.14	365.1	0.410	24.000
17	5	Plus Nutrients	N3	N	3	9.68E8	7.39E8	4.25	4.39	0.760	0.15	349.8	0.410	23.040
20	6	Plus Nutrients	N1	N	1	5.19E8	5.09E8	2.50	4.81	0.980	0.18	358.7	0.410	24.630
20	6	Plus Nutrients	N2	N	2	7.51E8	5.86E8	0.60	0.80	0.780	0.48	357.1	0.520	24.450
20	6	Plus Nutrients	N3	N	3	5.85E8	6.96E8	3.17	5.42	1.190	0.18	365.9	0.400	22.430
24	7	Plus Nutrients	N1	N	1	8.00E8	6.61E8	3.41	4.26	0.830	0.21	372.9	0.430	24.680
24	7	Plus Nutrients	N2	N	2	7.13E8	6.71E8	4.51	6.33	0.940	0.15	365.0	0.440	25.060
24	7	Plus Nutrients	N3	N	3	11.1E8	7.27E8	2.23	2.00	0.650	0.33	358.3	0.340	23.900
27	8	Plus Nutrients	N1	N	1	8.59E8	9.78E8	2.40	2.79	1.140	0.35	352.9	0.420	25.140
27	8	Plus Nutrients	N2	N	2	7.92E8	7.83E8	2.83	3.57	0.990	0.28	381.9	0.430	25.600
27	8	Plus Nutrients	N3	N	3	8.98E8	12.0E8	3.46	3.85	1.330	0.34	358.5	0.320	23.820
3	1	Plus Temperature	T1	T	1	11.8E8	36.3E6	1.27	1.08	0.030	0.03	365.0	0.280	19.190
3	1	Plus Temperature	T2	T	2	15.8E8	2.15E8	2.18	1.39	0.140	0.09	358.3	0.270	19.540
3	1	Plus Temperature	T3	T	3	12.2E8	1.87E8	1.90	1.56	0.150	0.08	370.9	0.280	18.620
6	2	Plus Temperature	T1	T	1	11.9E8	33.8E8	2.42	2.03	2.830	0.58	352.9	0.090	16.040
6	2	Plus Temperature	T2	T	2	13.0E8	21.9E8	12.72	9.82	1.690	0.12	364.3	0.100	15.480
6	2	Plus Temperature	T3	T	3	11.7E8	22.0E8	7.66	6.52	1.880	0.21	342.8	0.080	16.030
10	3	Plus Temperature	T1	T	1	11.3E8	13.9E8	2.64	2.34	1.230	0.36	348.8	0.110	16.030
10	3	Plus Temperature	T2	T	2	16.8E8	6.61E8	4.61	2.74	0.390	0.10	351.2	0.090	14.380
10	3	Plus Temperature	T3	T	3	11.6E8	12.0E8	4.32	3.71	1.030	0.23	349.0	0.110	16.650
13	4	Plus Temperature	T1	T	1	14.2E8	16.0E8	2.38	1.67	1.120	0.43	348.7	0.130	15.620
13	4	Plus Temperature	T2	T	2	17.2E8	11.4E8	3.38	1.97	0.670	0.25	348.2	0.120	14.550
13	4	Plus Temperature	T3	T	3	19.4E8	22.2E8	2.64	1.36	1.150	0.51	345.1	0.150	16.520
17	5	Plus Temperature	T1	T	1	28.0E8	22.5E8	2.28	0.81	0.800	0.55	327.1	0.100	15.140
17	5	Plus Temperature	T2	T	2	33.8E8	40.7E8	3.70	1.09	1.200	0.52	349.4	0.110	14.080
17	5	Plus Temperature	T3	T	3	27.7E8	46.6E8	7.46	2.70	1.680	0.43	334.8	0.120	15.330
20	6	Plus Temperature	T1	T	1	27.6E8	53.4E8	4.97	1.80	1.930	0.56	349.3	0.100	15.040
20	6	Plus Temperature	T2	T	2	34.3E8	27.3E8	2.42	0.71	0.800	0.52	334.9	0.090	13.820
20	6	Plus Temperature	T3	T	3	24.7E8	47.6E8	4.32	1.75	1.920	0.58	330.2	0.090	14.380
24	7	Plus Temperature	T1	T	1	25.5E8	29.9E8	1.80	0.70	1.170	0.65	333.0	0.090	15.030
24	7	Plus Temperature	T2	T	2	31.1E8	23.4E8	2.21	0.71	0.750	0.50	335.1	0.070	14.330
24	7	Plus Temperature	T3	T	3	18.4E8	12.0E8	1.46	0.79	0.650	0.45	331.4	0.110	16.140
27	8	Plus Temperature	T1	T	1	21.9E8	6.34E8	0.70	0.32	0.290	0.46	369.8	0.100	15.310
27	8	Plus Temperature	T2	T	2	27.0E8	12.5E8	3.50	1.30	0.460	0.25	338.0	0.090	14.060
27	8	Plus Temperature	T3	T	3	19.6E8	13.1E8	2.69	1.37	0.670	0.34	381.2	0.110	15.480
3	1	Plus Nutrients and Temperature	TN1	TN	1	12.7E8	15.8E8	4.80	3.77	1.240	0.26	367.9	0.250	18.410
3	1	Plus Nutrients and Temperature	TN2	TN	2	15.2E8	17.7E6	5.52	3.64	0.010	0.00	335.1	0.280	18.670
3	1	Plus Nutrients and Temperature	TN3	TN	3	20.7E8	18.9E8	3.46	1.67	0.910	0.38	331.4	0.260	18.620
6	2	Plus Nutrients and Temperature	TN1	TN	1	20.8E8	12.3E8	2.57	1.24	0.590	0.28	338.0	0.120	15.810
6	2	Plus Nutrients and Temperature	TN2	TN	2	18.4E8	77.0E8	9.82	5.32	4.170	0.48	381.2	0.130	17.030
6	2	Plus Nutrients and Temperature	TN3	TN	3	21.6E8	66.9E8	6.22	2.87	3.090	0.56	380.2	0.150	17.290
10	3	Plus Nutrients and Temperature	TN1	TN	1	9.28E8	18.6E8	3.53	3.80	2.000	0.36	342.9	0.150	15.960
10	3	Plus Nutrients and Temperature	TN2	TN	2	13.1E8	15.6E8	8.21	6.28	1.200	0.20	359.2	0.240	18.650
10	3	Plus Nutrients and Temperature	TN3	TN	3	18.9E8	45.8E8	7.49	3.96	2.420	0.46	342.2	0.180	18.480
13	4	Plus Nutrients and Temperature	TN1	TN	1	11.0E8	28.3E8	4.30	3.89	2.560	0.44	351.9	0.190	17.130
13	4	Plus Nutrients and Temperature	TN2	TN	2	24.1E8	57.0E8	7.22	3.00	2.370	0.51	353.2	0.320	20.240
13	4	Plus Nutrients and Temperature	TN3	TN	3	29.1E8	61.1E8	4.18	1.44	2.100	0.67	352.8	0.290	19.600
17	5	Plus Nutrients and Temperature	TN1	TN	1	20.1E8	61.6E8	4.90	2.44	3.070	0.60	351.3	0.140	15.420
17	5	Plus Nutrients and Temperature	TN2	TN	2	47.9E8	1.09E10	11.93	2.49	2.280	0.55	345.6	0.210	19.500
17	5	Plus Nutrients and Temperature	TN3	TN	3	48.2E8	1.32E10	13.18	2.73	2.730	0.57	346.2	0.210	19.880
20	6	Plus Nutrients and Temperature	TN1	TN	1	19.5E8	57.3E8	5.28	2.71	2.940	0.57	337.6	0.200	16.260
20	6	Plus Nutrients and Temperature	TN2	TN	2	22.6E8	10.5E8	3.91	1.73	0.460	0.22	345.8	0.380	21.860
20	6	Plus Nutrients and Temperature	TN3	TN	3	21.4E8	21.8E8	2.23	1.04	1.020	0.48	346.4	0.350	22.110
24	7	Plus Nutrients and Temperature	TN1	TN	1	23.3E8	13.9E8	1.92	0.82	0.590	0.38	342.0	0.190	18.620
24	7	Plus Nutrients and Temperature	TN2	TN	2	17.6E8	7.42E8	2.11	1.20	0.420	0.24	352.8	0.530	24.370
24	7	Plus Nutrients and Temperature	TN3	TN	3	19.2E8	18.8E8	1.99	1.04	0.980	0.45	350.4	0.360	22.670
27	8	Plus Nutrients and Temperature	TN1	TN	1	27.2E8	12.8E8	2.26	0.83	0.470	0.37	340.8	0.270	20.590
27	8	Plus Nutrients and Temperature	TN2	TN	2	23.0E8	11.9E8	3.79	1.65	0.520	0.23	343.9	0.570	24.810
27	8	Plus Nutrients and Temperature	TN3	TN	3	24.1E8	34.0E8	7.92	3.28	1.410	0.27	344.7	0.480	24.320