Rahlff, Janina; Giebel, Helge-Ansgar; Lehners, Carola; Ribas-Ribas, Mariana; Wurl, Oliver; Bergström, Kristofer; Holmfeldt, Karin (2023): Natural sea surface slicks from the Baltic Sea investigated for cell and virus-like particle abundance, surfactants and dissolved organic carbon in May 2021 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.955904
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Abstract:
Flow cytometry samples for the measurement of virus-like particles and prokaryotic abundance were sampled from the sea-surface microlayer inside and outside a natural surface slick and respective underlying water samples at three sites in the Kalmar Sound, near Rockneby, Sweden, using a boat. A glass plate sampler and a Ruttner sampler were used to sample the sea-surface microlayer and underlying water from 70 cm depth, respectively. For site 1, dissolved organic carbon and surfactants were additionally measured in all four sample types. Samples for flow cytometry were fixed with glutardialdehyde (0.5% final concentration). Some metadata for wind speed, temperature and salinity were collected. These data complement metagenomic analysis of viral communities in the sea-surface microlayer of a natural surface slick (Rahlff et al., unpublished).
Supplement to:
Rahlff, Janina; Wietz, Matthias; Giebel, Helge-Ansgar; Bayfield, Oliver; Nilsson, Emelie; Bergström, Kristofer; Kieft, Kristopher; Anantharaman, Karthik; Ribas-Ribas, Mariana; Schweitzer, Hannah D; Wurl, Oliver; Hoetzinger, Matthias; Antson, Alfred; Holmfeldt, Karin (2023): Ecogenomics and cultivation reveal distinctive viral-bacterial communities in the surface microlayer of a Baltic Sea slick. ISME Communications, 3, 97, https://doi.org/10.1038/s43705-023-00307-8
References:
Martínez-García, Sandra; Bunse, Carina; Pontiller, Benjamin; Baltar, Federico; Israelsson, Stina; Fridolfsson, Emil; Lindh, Markus V; Lundin, Daniel; Legrand, Catherine; Pinhassi, Jarone (2022): Seasonal Dynamics in Carbon Cycling of Marine Bacterioplankton Are Lifestyle Dependent. Frontiers in Microbiology, 13, 834675, https://doi.org/10.3389/fmicb.2022.834675
Funding:
German Research Foundation (DFG), grant/award no. 446702140: VIBOCAT - Erforschung des Virioneustons: Viral-bakterielle Interaktionen zwischen Ozean und Atmosphäre
Coverage:
Median Latitude: 56.837553 * Median Longitude: 16.467317 * South-bound Latitude: 56.832660 * West-bound Longitude: 16.447770 * North-bound Latitude: 56.841470 * East-bound Longitude: 16.479770
Date/Time Start: 2021-05-31T07:10:00 * Date/Time End: 2021-05-31T09:50:00
Minimum DEPTH, water: 0.001 m * Maximum DEPTH, water: 0.700 m
Event(s):
Kalmarsund_1 * Latitude Start: 56.832660 * Longitude Start: 16.447770 * Latitude End: 56.832690 * Longitude End: 16.448530 * Date/Time Start: 2021-05-31T07:10:00 * Date/Time End: 2021-05-31T08:10:00 * Location: Kalmar Strait * Method/Device: Multiple investigations (MULT)
Comment:
The virus-like particles and prokaryote abundance samples had one unwanted freeze thaw cycle due to shipment problems. At best, this data set should not be compared with others, just comparisons within this dataset should be done.
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Rahlff, Janina | |||
| 2 | Station label | Station | Rahlff, Janina | |||
| 3 | Sample type | Samp type | Rahlff, Janina | |||
| 4 | DATE/TIME | Date/Time | Rahlff, Janina | Geocode – start (UTC) | ||
| 5 | DATE/TIME | Date/Time | Rahlff, Janina | Geocode – end (UTC) | ||
| 6 | Date/Time local | Date/Time local | Rahlff, Janina | start | ||
| 7 | Date/Time local | Date/Time local | Rahlff, Janina | end | ||
| 8 | LATITUDE | Latitude | Rahlff, Janina | Geocode | ||
| 9 | LONGITUDE | Longitude | Rahlff, Janina | Geocode | ||
| 10 | Virus-like particles | Virus-like | #/ml | Rahlff, Janina | Flow cytometer, BD Biosciences, BD Accuri C6 | |
| 11 | Prokaryotes | Prokaryotes | #/ml | Rahlff, Janina | Flow cytometer, BD Biosciences, BD Accuri C6 | Prokaryotic cells |
| 12 | Surfactants | Surfact | µg/l | Rahlff, Janina | Voltammetry stand, Metrohm, 747 VA Computrace | µg Teq L⁻¹ (Teq = Triton-X-100 equivalent) |
| 13 | Carbon, organic, dissolved | DOC | mg/l | Rahlff, Janina | Dissolved organic carbon analysis according to Martínez-García et al. (2022) | |
| 14 | Habitat | Habitat | Rahlff, Janina | |||
| 15 | DEPTH, water | Depth water | m | Rahlff, Janina | Geocode | |
| 16 | Sample method | Sample method | Rahlff, Janina | |||
| 17 | Wind speed | ff | m/s | Rahlff, Janina | Digital anemometer, MASTECH, MS6252A | during sampling |
| 18 | Temperature, water | Temp | °C | Rahlff, Janina | Multi-parameter portable meter, Xylem Analytics, MultiLine® Multi 3620 IDS | |
| 19 | Salinity | Sal | Rahlff, Janina | Multi-parameter portable meter, Xylem Analytics, MultiLine® Multi 3620 IDS | Seawater | |
| 20 | Comment | Comment | Rahlff, Janina | Notes |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
138 data points
Data
| 1 Event | 2 Station | 3 Samp type | 4 Date/Time (start (UTC)) | 5 Date/Time (end (UTC)) | 6 Date/Time local (start) | 7 Date/Time local (end) | 8 Latitude | 9 Longitude | 10 Virus-like [#/ml] (Flow cytometer, BD Bioscience...) | 11 Prokaryotes [#/ml] (Prokaryotic cells, Flow cytom...) | 12 Surfact [µg/l] (µg Teq L⁻¹ (Teq = Triton-X-10...) | 13 DOC [mg/l] (Dissolved organic carbon anal...) | 14 Habitat | 15 Depth water [m] | 16 Sample method | 17 ff [m/s] (during sampling, Digital anem...) | 18 Temp [°C] (Multi-parameter portable mete...) | 19 Sal (Seawater, Multi-parameter por...) | 20 Comment (Notes) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Kalmarsund_1 | 1 | Microlayer from slick | 2021-05-31T07:10 | 2021-05-31T07:40 | 2021-05-31T08:10 | 2021-05-31T08:40 | 56.83266 | 16.44777 | 172367320.0 | 4111530.6 | 1219.3 | 7.76 | Surface microlayer | 0.001 | Glass plate sampler | 1.5 | 13.3 | 6.6 | |
| Kalmarsund_1 | 1 | Microlayer from non-slick | 2021-05-31T07:40 | 2021-05-31T08:10 | 2021-05-31T08:40 | 2021-05-31T09:10 | 56.83269 | 16.44853 | 12557077.0 | 624468.7 | 412.1 | 5.36 | Surface microlayer | 0.001 | Glass plate sampler | 4.2 | 12.8 | 6.6 | some sea weeds floating in the water |
| Kalmarsund_1 | 1 | Underlying water slick | 2021-05-31T07:10 | 2021-05-31T07:40 | 2021-05-31T08:10 | 2021-05-31T08:40 | 56.83266 | 16.44777 | 26980841.5 | 685400.6 | 392.4 | 5.09 | Subsurface water (70 cm depth) | 0.700 | 1-L Ruttner Sampler | 1.5 | 13.3 | 6.6 | |
| Kalmarsund_1 | 1 | Underlying water non-slick | 2021-05-31T07:40 | 2021-05-31T08:10 | 2021-05-31T08:40 | 2021-05-31T09:10 | 56.83269 | 16.44853 | 12691488.1 | 574195.0 | 334.3 | 5.09 | Subsurface water (70 cm depth) | 0.700 | 1-L Ruttner Sampler | 4.2 | 12.8 | 6.6 | some sea weeds floating in the water |
| Kalmarsund_2 | 2 | Microlayer from slick | 2021-05-31T08:30 | 2021-05-31T08:50 | 2021-05-31T09:30 | 2021-05-31T09:50 | 56.84147 | 16.47345 | 119550032.5 | 2239784.6 | Surface microlayer | 0.001 | Glass plate sampler | 3.3 | 12.6 | 6.6 | |||
| Kalmarsund_2 | 2 | Microlayer from non-slick | 2021-05-31T09:00 | 2021-05-31T09:20 | 2021-05-31T10:00 | 2021-05-31T10:20 | 56.84128 | 16.47461 | 13321699.3 | 555695.4 | Surface microlayer | 0.001 | Glass plate sampler | 3.3 | 12.6 | 6.6 | |||
| Kalmarsund_2 | 2 | Underlying water slick | 2021-05-31T08:30 | 2021-05-31T08:50 | 2021-05-31T09:30 | 2021-05-31T09:50 | 56.84147 | 16.47345 | 24544216.3 | 539773.2 | Subsurface water (70 cm depth) | 0.700 | 1-L Ruttner Sampler | 3.3 | 12.6 | 6.6 | |||
| Kalmarsund_2 | 2 | Underlying water non-slick | 2021-05-31T09:00 | 2021-05-31T09:20 | 2021-05-31T10:00 | 2021-05-31T10:20 | 56.84128 | 16.47461 | 19599474.1 | 625619.8 | Subsurface water (70 cm depth) | 0.700 | 1-L Ruttner Sampler | 3.3 | 12.6 | 6.6 | |||
| Kalmarsund_3 | 3 | Microlayer from slick | 2021-05-31T09:30 | 2021-05-31T09:40 | 2021-05-31T10:30 | 2021-05-31T10:40 | 56.83861 | 16.47977 | 64740705.3 | 1686583.5 | Surface microlayer | 0.001 | Glass plate sampler | 3.7 | 6.6 | ||||
| Kalmarsund_3 | 3 | Microlayer from non-slick | 2021-05-31T09:40 | 2021-05-31T09:50 | 2021-05-31T10:40 | 2021-05-31T10:50 | 56.83861 | 16.47977 | 15176982.2 | 582883.6 | Surface microlayer | 0.001 | Glass plate sampler | 3.7 | 6.6 | ||||
| Kalmarsund_3 | 3 | Underlying water slick | 2021-05-31T09:30 | 2021-05-31T09:40 | 2021-05-31T10:30 | 2021-05-31T10:40 | 56.83861 | 16.47977 | 9512319.8 | 508178.4 | Subsurface water (70 cm depth) | 0.700 | 1-L Ruttner Sampler | 3.7 | 6.6 | ||||
| Kalmarsund_3 | 3 | Underlying water non-slick | 2021-05-31T09:40 | 2021-05-31T09:50 | 2021-05-31T10:40 | 2021-05-31T10:50 | 56.83861 | 16.47977 | 9148041.5 | 553616.8 | Subsurface water (70 cm depth) | 0.700 | 1-L Ruttner Sampler | 3.7 | 6.6 |