Koch, Marie; Tebben, Jan; Saborowski, Reinhard (2023): Lipid metabolism in Decapods: activity of the enzyme diacylglycerol acyltransferase (DGAT) [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.955469, In: Koch, M et al. (2023): Lipid metabolism in Decapods: DGAT activity, total lipid content, transcripts of relevant enzymes [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.955471
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Published: 2023-02-10 • DOI registered: 2023-04-01
References:
McFie, Pamela J; Stone, Scot J (2011): A fluorescent assay to quantitatively measure in vitro acyl CoA:diacylglycerol acyltransferase activity. Journal of Lipid Research, 52(9), 1760-1764, https://doi.org/10.1194/jlr.D016626
Coverage:
Median Latitude: 53.855557 * Median Longitude: 8.127777 * South-bound Latitude: 53.716670 * West-bound Longitude: 7.916670 * North-bound Latitude: 54.133330 * East-bound Longitude: 8.233330
Date/Time Start: 2021-06-30T00:00:00 * Date/Time End: 2021-08-21T00:00:00
Event(s):
UT06/2021_trawl0630 * Latitude: 53.716670 * Longitude: 8.233330 * Date/Time: 2021-06-30T00:00:00 * Location: North Sea * Campaign: UT06/2021 * Basis: Uthörn * Method/Device: Trawl net (TRAWL)
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Species, unique identification | Species UID | Koch, Marie | |||
| 2 | Species, unique identification (URI) | Species UID (URI) | Koch, Marie | |||
| 3 | Season | Season | Koch, Marie | |||
| 4 | Method comment | Method comm | Koch, Marie | Mikrosome | ||
| 5 | Protein | Protein | µg/µl | Koch, Marie | Bradford (Bio-Rad, 500.0006) | |
| 6 | Triacylglycerols | TAG | arbitrary units | Koch, Marie | assay product, fluorescence units | |
| 7 | Treatment: duration | T:duration | min | Koch, Marie | reaction time | |
| 8 | Treatment: temperature | T:temp | °C | Koch, Marie | After McFie PJ and Stone SJ (2011) | |
| 9 | Diacylglycerol acyltransferase, activity per protein mass | DGAT/prot | arbitrary units/min/mg | Koch, Marie | Bio-Rad Gel-DocTM EZ Imager, with ImageLab Software |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
80 data points
Data
| 1 Species UID | 2 Species UID (URI) | 3 Season | 4 Method comm | 5 Protein [µg/µl] | 6 TAG [arbitrary units] | 7 T:duration [min] | 8 T:temp [°C] | 9 DGAT/prot [arbitrary units/min/mg] |
|---|---|---|---|---|---|---|---|---|
| Crangon crangon | marinespecies.org | Summer | E13 | 51.504 | 6010.44 | 30 | 37 | 3.89 |
| Crangon crangon | marinespecies.org | Summer | E14 | 51.543 | 11587.89 | 30 | 37 | 7.49 |
| Crangon crangon | marinespecies.org | Summer | E17 | 62.778 | 179511.89 | 30 | 37 | 95.32 |
| Crangon crangon | marinespecies.org | Summer | E20 | 55.978 | 214606.33 | 30 | 37 | 127.79 |
| Crangon crangon | marinespecies.org | Summer | E21 | 81.267 | 376988.00 | 30 | 37 | 154.63 |
| Pandalus montagui | marinespecies.org | Summer | E7 | 40.667 | 155319.22 | 30 | 37 | 127.31 |
| Pandalus montagui | marinespecies.org | Summer | E8 | 46.900 | 384881.44 | 30 | 37 | 273.55 |
| Pandalus montagui | marinespecies.org | Summer | E18 | 59.294 | 528070.33 | 30 | 37 | 296.86 |
| Pandalus montagui | marinespecies.org | Summer | E22 | 70.283 | 550275.56 | 30 | 37 | 260.98 |
| Pandalus montagui | marinespecies.org | Summer | E23 | 50.861 | 148174.56 | 30 | 37 | 97.11 |