Brennan, Reid S; deMayo, James A; Dam, Hans G; Finiguerra, Michael B; Baumann, Hannes; Buffalo, Vince; Pespeni, Melissa H (2022): Seawater carbonate chemistry for the transgenerational experiment on synergistic genomic mechanisms of adaptation to ocean warming and acidification in a marine copepod [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.953111
Always quote citation above when using data! You can download the citation in several formats below.
Abstract:
Metazoan adaptation to global change relies on selection of standing genetic variation. Determining the extent to which this variation exists in natural populations, particularly for responses to simultaneous stressors, is essential to make accurate predictions for persistence in future conditions. Here, we identified the genetic variation enabling the copepod Acartia tonsa to adapt to experimental ocean warming, acidification, and combined ocean warming and acidification (OWA) over 25 generations of continual selection. Replicate populations showed a consistent polygenic response to each condition, targeting an array of adaptive mechanisms including cellular homeostasis, development, and stress response. We used a genome-wide covariance approach to partition the allelic changes into three categories: selection, drift and replicate-specific selection, and laboratory adaptation responses. The majority of allele frequency change in warming (57%) and OWA (63%) was driven by shared selection pressures across replicates, but this effect was weaker under acidification alone (20%). OWA and warming shared 37% of their response to selection but OWA and acidification shared just 1%, indicating that warming is the dominant driver of selection in OWA. Despite the dominance of warming, the interaction with acidification was still critical as the OWA selection response was highly synergistic with 47% of the allelic selection response unique from either individual treatment. These results disentangle how genomic targets of selection differ between single and multiple stressors and demonstrate the complexity that nonadditive multiple stressors will contribute to predictions of adaptation to complex environmental shifts caused by global change.
Keyword(s):
Supplement to:
Brennan, Reid S; deMayo, James A; Dam, Hans G; Finiguerra, Michael B; Baumann, Hannes; Buffalo, Vince; Pespeni, Melissa H (2022): Experimental evolution reveals the synergistic genomic mechanisms of adaptation to ocean warming and acidification in a marine copepod. Proceedings of the National Academy of Sciences, 119(38), e2201521119, https://doi.org/10.1073/pnas.2201521119
Further details:
Brennan, Reid S (2022): Acartia tonsa 25 generation experimental evolution. Zenodo, https://doi.org/10.5281/zenodo.5093796
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html
Project(s):
Coverage:
Latitude: 41.320725 * Longitude: -72.001643
Date/Time Start: 2016-06-01T00:00:00 * Date/Time End: 2016-06-30T00:00:00
Event(s):
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-12-23.
Parameter(s):
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
576 data points
Data
1 Type (Study) | 2 Species UID | 3 Species UID (URI) | 4 Species UID (Semantic URI) | 5 Measurement ID | 6 T:temp [°C] | 7 T:pH | 8 T:pCO2 [µatm] | 9 Sal | 10 AT [µmol/kg] | 11 AT std dev [±] | 12 AT std e [±] | 13 pCO2water_SST_wet [µatm] (Calculated using CO2SYS) | 14 pCO2 std dev [±] (Calculated using CO2SYS) | 15 pCO2water_SST_wet std e [±] (Calculated using CO2SYS) | 16 fCO2water_SST_wet [µatm] (Calculated using CO2SYS) | 17 fCO2 std dev [±] (Calculated using CO2SYS) | 18 fCO2 std e [±] (Calculated using CO2SYS) | 19 Omega Arg (Calculated using CO2SYS) | 20 Omega Arg std dev [±] (Calculated using CO2SYS) | 21 Omega Arg std e [±] (Calculated using CO2SYS) | 22 Omega Cal (Calculated using CO2SYS) | 23 Omega Cal std dev [±] (Calculated using CO2SYS) | 24 Omega Cal std e [±] (Calculated using CO2SYS) | 25 DIC [µmol/kg] (Calculated using CO2SYS) | 26 DIC std dev [±] (Calculated using CO2SYS) | 27 DIC std e [±] (Calculated using CO2SYS) | 28 Repl [#] | 29 Temp [°C] | 30 Temp std dev [±] | 31 Repl [#] | 32 T std e [±] | 33 pH (NBS scale) | 34 pH std dev [±] (NBS scale) | 35 Repl [#] | 36 pH std e [±] | 37 pCO2water_SST_wet [µatm] | 38 pCO2 std dev [±] | 39 Repl [#] | 40 pCO2water_SST_wet std e [±] | 41 CSC flag (Calculated using seacarb afte...) | 42 pH (total scale, Calculated using...) | 43 CO2 [µmol/kg] (Calculated using seacarb afte...) | 44 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...) | 45 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...) | 46 [HCO3]- [µmol/kg] (Calculated using seacarb afte...) | 47 [CO3]2- [µmol/kg] (Calculated using seacarb afte...) | 48 DIC [µmol/kg] (Calculated using seacarb afte...) | 49 Omega Arg (Calculated using seacarb afte...) | 50 Omega Cal (Calculated using seacarb afte...) |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
laboratory | Acartia tonsa cryophylla | marinespecies.org | urn:lsid:marinespecies.org:taxname:362901 | 1 | 18 | 8.2 | 400 | 31 | 2086 | 10 | 6 | 348 | 2 | 1 | 347 | 2 | 1 | 2.40 | 0.200 | 0.009 | 3.80 | 0.020 | 0.010 | 1872 | 9 | 5 | 3 | 18.08 | 0.368310 | 165 | 0.028673 | 8.28 | 0.101190 | 165 | 0.007878 | 380.94 | 49.29688 | 9 | 16.432290 | 26 | 8.15 | 9.70 | 277.50 | 278.47 | 1644.27 | 178.12 | 1832.10 | 2.80 | 4.37 |
laboratory | Acartia tonsa cryophylla | marinespecies.org | urn:lsid:marinespecies.org:taxname:362901 | 1 | 18 | 7.5 | 2000 | 31 | 2106 | 10 | 6 | 2062 | 10 | 6 | 2056 | 10 | 6 | 0.57 | 0.006 | 0.003 | 0.88 | 0.006 | 0.003 | 2125 | 10 | 6 | 3 | 17.95 | 0.302756 | 165 | 0.023570 | 7.53 | 0.082598 | 165 | 0.006430 | 2245.09 | 276.60750 | 9 | 95.202490 | 26 | 7.40 | 66.92 | 1906.58 | 1913.25 | 2010.64 | 38.54 | 2116.10 | 0.61 | 0.95 |
laboratory | Acartia tonsa cryophylla | marinespecies.org | urn:lsid:marinespecies.org:taxname:362901 | 1 | 22 | 8.2 | 400 | 31 | 2146 | 23 | 13 | 366 | 4 | 2 | 364 | 4 | 2 | 2.80 | 0.040 | 0.020 | 4.30 | 0.050 | 0.030 | 1902 | 21 | 12 | 3 | 21.90 | 1.098083 | 167 | 0.084972 | 8.25 | 0.104003 | 167 | 0.008048 | 377.17 | 17.67343 | 9 | 5.891142 | 26 | 8.12 | 9.94 | 316.17 | 317.22 | 1674.52 | 191.84 | 1876.30 | 3.07 | 4.74 |
laboratory | Acartia tonsa cryophylla | marinespecies.org | urn:lsid:marinespecies.org:taxname:362901 | 1 | 22 | 7.5 | 2000 | 31 | 2170 | 10 | 6 | 2209 | 10 | 6 | 2201 | 10 | 6 | 0.68 | 0.006 | 0.003 | 1.00 | 0.006 | 0.003 | 2178 | 10 | 6 | 3 | 21.94 | 0.334943 | 169 | 0.025765 | 7.57 | 0.079519 | 169 | 0.006117 | 2358.12 | 120.80670 | 9 | 40.268890 | 26 | 7.44 | 58.21 | 1853.68 | 1859.85 | 2049.77 | 49.12 | 2157.10 | 0.79 | 1.21 |
laboratory | Acartia tonsa cryophylla | marinespecies.org | urn:lsid:marinespecies.org:taxname:362901 | 2 | 18 | 8.2 | 400 | 36 | 2830 | 119 | 69 | 446 | 10 | 6 | 445 | 10 | 6 | 3.60 | 0.300 | 0.100 | 5.60 | 0.400 | 0.200 | 2516 | 92 | 53 | 3 | 18.08 | 0.368310 | 165 | 0.028673 | 8.28 | 0.101190 | 165 | 0.007878 | 380.94 | 49.29688 | 9 | 16.432290 | 26 | 8.16 | 12.13 | 356.43 | 357.67 | 2183.37 | 270.70 | 2466.20 | 4.16 | 6.42 |
laboratory | Acartia tonsa cryophylla | marinespecies.org | urn:lsid:marinespecies.org:taxname:362901 | 2 | 18 | 7.5 | 2000 | 35 | 2748 | 19 | 15 | 2613 | 31 | 17 | 2604 | 30 | 17 | 0.81 | 0.010 | 0.007 | 1.20 | 0.020 | 0.010 | 2764 | 20 | 12 | 3 | 17.95 | 0.302756 | 165 | 0.023570 | 7.53 | 0.082598 | 165 | 0.006430 | 2245.09 | 276.60750 | 9 | 95.202490 | 26 | 7.41 | 82.85 | 2412.90 | 2421.35 | 2614.39 | 55.85 | 2753.10 | 0.86 | 1.33 |
laboratory | Acartia tonsa cryophylla | marinespecies.org | urn:lsid:marinespecies.org:taxname:362901 | 2 | 22 | 8.2 | 400 | 33 | 2401 | 27 | 15 | 400 | 5 | 3 | 399 | 5 | 3 | 3.30 | 0.040 | 0.020 | 5.00 | 0.060 | 0.030 | 2121 | 25 | 14 | 3 | 21.90 | 1.098083 | 167 | 0.084972 | 8.25 | 0.104003 | 167 | 0.008048 | 377.17 | 17.67343 | 9 | 5.891142 | 26 | 8.12 | 10.75 | 345.52 | 346.67 | 1856.17 | 224.58 | 2091.50 | 3.56 | 5.47 |
laboratory | Acartia tonsa cryophylla | marinespecies.org | urn:lsid:marinespecies.org:taxname:362901 | 2 | 22 | 7.5 | 2000 | 35 | 2466 | 56 | 33 | 2441 | 66 | 38 | 2433 | 66 | 38 | 0.82 | 0.020 | 0.009 | 1.30 | 0.020 | 0.009 | 2466 | 59 | 33 | 3 | 21.94 | 0.334943 | 169 | 0.025765 | 7.57 | 0.079519 | 169 | 0.006117 | 2358.12 | 120.80670 | 9 | 40.268890 | 26 | 7.44 | 62.74 | 2040.94 | 2047.73 | 2316.21 | 61.75 | 2440.70 | 0.97 | 1.48 |
laboratory | Acartia tonsa cryophylla | marinespecies.org | urn:lsid:marinespecies.org:taxname:362901 | 3 | 18 | 8.2 | 400 | 31 | 2095 | 6 | 3 | 348 | 3 | 2 | 348 | 3 | 2 | 2.40 | 0.020 | 0.010 | 3.80 | 0.030 | 0.020 | 1877 | 9 | 5 | 3 | 18.08 | 0.368310 | 165 | 0.028673 | 8.28 | 0.101190 | 165 | 0.007878 | 380.94 | 49.29688 | 9 | 16.432290 | 26 | 8.15 | 9.74 | 278.68 | 279.65 | 1651.60 | 178.95 | 1840.30 | 2.82 | 4.39 |
laboratory | Acartia tonsa cryophylla | marinespecies.org | urn:lsid:marinespecies.org:taxname:362901 | 3 | 18 | 7.5 | 2000 | 31 | 2103 | 6 | 3 | 2059 | 6 | 3 | 2052 | 6 | 3 | 0.57 | 0.006 | 0.003 | 0.89 | 0.006 | 0.003 | 2121 | 6 | 3 | 3 | 17.95 | 0.302756 | 165 | 0.023570 | 7.53 | 0.082598 | 165 | 0.006430 | 2245.09 | 276.60750 | 9 | 95.202490 | 26 | 7.40 | 66.85 | 1904.49 | 1911.16 | 2007.78 | 38.47 | 2113.10 | 0.61 | 0.94 |
laboratory | Acartia tonsa cryophylla | marinespecies.org | urn:lsid:marinespecies.org:taxname:362901 | 3 | 22 | 8.2 | 400 | 33 | 2200 | 11 | 6 | 366 | 2 | 1 | 364 | 2 | 1 | 3.00 | 0.020 | 0.009 | 4.60 | 0.020 | 0.010 | 1936 | 10 | 6 | 3 | 21.90 | 1.098083 | 167 | 0.084972 | 8.25 | 0.104003 | 167 | 0.008048 | 377.17 | 17.67343 | 9 | 5.891142 | 26 | 8.12 | 9.81 | 315.48 | 316.53 | 1694.42 | 204.97 | 1909.20 | 3.25 | 4.99 |
laboratory | Acartia tonsa cryophylla | marinespecies.org | urn:lsid:marinespecies.org:taxname:362901 | 3 | 22 | 7.5 | 2000 | 34 | 2443 | 62 | 36 | 2422 | 60 | 35 | 2416 | 60 | 34 | 0.81 | 0.030 | 0.020 | 1.20 | 0.040 | 0.020 | 2444 | 62 | 36 | 3 | 21.94 | 0.334943 | 169 | 0.025765 | 7.57 | 0.079519 | 169 | 0.006117 | 2358.12 | 120.80670 | 9 | 40.268890 | 26 | 7.44 | 62.97 | 2037.57 | 2044.35 | 2298.44 | 59.69 | 2421.10 | 0.94 | 1.44 |