Citation:

PANGAEA.951332

Name: Seawater carbonate chemistry and the decrease of H+ concentration in the phycosphere and thickness of the pH boundary layer of marine diatoms Coscinodiscus wailesii
Published: 2022-11-29
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Acid-base regulation; Bottles or small containers/Aquaria (<20 L); Chromista; Coscinodiscus wailesii; Laboratory experiment; Laboratory strains; Not applicable; Ochrophyta; Pelagos; Phytoplankton; Single species

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Abstract:

Surface ocean pH is declining due to anthropogenic atmospheric CO2 uptake with a global decline of ~0.3 possible by 2100. Extracellular pH influences a range of biological processes, including nutrient uptake, calcification and silicification. However, there are poor constraints on how pH levels in the extracellular microenvironment surrounding phytoplankton cells (the phycosphere) differ from bulk seawater. This adds uncertainty to biological impacts of environmental change. Furthermore, previous modelling work suggests that phycosphere pH of small cells is close to bulk seawater, and this has not been experimentally verified. Here we observe under 140 μmol photons/m**2/s the phycosphere pH of Chlamydomonas concordia (5 µm diameter), Emiliania huxleyi (5 µm), Coscinodiscus radiatus (50 µm) and C. wailesii (100 µm) are 0.11 ± 0.07, 0.20 ± 0.09, 0.41 ± 0.04 and 0.15 ± 0.20 (mean ± SD) higher than bulk seawater (pH 8.00), respectively. Thickness of the pH boundary layer of C. wailesii increases from 18 ± 4 to 122 ± 17 µm when bulk seawater pH decreases from 8.00 to 7.78. Phycosphere pH is regulated by photosynthesis and extracellular enzymatic transformation of bicarbonate, as well as being influenced by light intensity and seawater pH and buffering capacity. The pH change alters Fe speciation in the phycosphere, and hence Fe availability to phytoplankton is likely better predicted by the phycosphere, rather than bulk seawater. Overall, the precise quantification of chemical conditions in the phycosphere is crucial for assessing the sensitivity of marine phytoplankton to ongoing ocean acidification and Fe limitation in surface oceans.

Keyword(s):

Acid-base regulation; Bottles or small containers/Aquaria (<20 L); Chromista; Coscinodiscus wailesii; Laboratory experiment; Laboratory strains; Not applicable; Ochrophyta; Pelagos; Phytoplankton; Single species

Supplement to:

Liu, Fengjie; Gledhill, Martha; Tan, Qiaoguo; Zhu, Kechen; Zhang, Qiong; Salaün, Pascal; Tagliabue, Alessandro; Zhang, Yanjun; Weiss, Dominik J; Achterberg, Eric Pieter; Korchev, Yuri (2022): Phycosphere pH of unicellular nano- and micro- phytoplankton cells and consequences for iron speciation. The ISME Journal, 16(10), 2329-2336, https://doi.org/10.1038/s41396-022-01280-1

Original version:

Liu, Fengjie (2022): Data for the phycosphere pH of unicellular nano- and micro- phytoplankton cells and consequences for iron speciation. figshare, https://doi.org/10.6084/m9.figshare.19576477.v1

Further details:

Raw data for the phycosphere pH of unicellular nano- and micro- phytoplankton cells and consequences for iron speciation

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-11-25.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Liu, Fengjie		Study
2	Species, unique identification	Species UID		Liu, Fengjie
3	Figure	Fig		Liu, Fengjie
4	Treatment	Treat		Liu, Fengjie
5	Proton gradients	Δ [H+]	nmol/l	Liu, Fengjie		decrease of H+ concentration in the phycosphere
6	Thickness	Thick	µm	Liu, Fengjie		pH boundary layer
7	Temperature, water	Temp	°C	Liu, Fengjie
8	Salinity	Sal		Liu, Fengjie
9	Alkalinity, total	AT	µmol/kg	Liu, Fengjie
10	Carbon, inorganic, dissolved	DIC	µmol/kg	Liu, Fengjie
11	Hydrogen ion concentration	H+	nmol/l	Liu, Fengjie
12	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
13	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
14	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
15	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
16	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
17	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
18	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
19	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
20	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

3286 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type	2 Species UID	3 Fig	4 Treat	5 Δ [H+] [nmol/l]	6 Thick [µm]	7 Temp [°C]	8 Sal	9 AT [µmol/kg]	10 DIC [µmol/kg]	11 H+ [nmol/l]	12 CSC flag	13 pH	14 CO2 [µmol/kg]	15 fCO2water_SST_wet [µatm]	16 pCO2water_SST_wet [µatm]	17 [HCO3]- [µmol/kg]	18 [CO3]2- [µmol/kg]	19 Omega Arg	20 Omega Cal
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	0.000000000051		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	0.000000000027		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	-0.000000000038		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	-0.000000000032		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	0.000000000071		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	-0.000000000078		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	-0.000000000218		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	-0.000000000029		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	0.000000000016		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	-0.000000000023		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	-0.000000000134		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	0.000000000098		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	0.000000000032		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	0.000000000056		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	-0.000000000105		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	-0.000000000078		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	0.000000000071		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	-0.000000000017		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	0.000000000076		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	-0.000000000032		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	-0.000000000066		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	-0.000000000057		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	-0.000000000098		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	-0.000000000167		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	-0.000000000045		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	0.000000000021		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	0.000000000026		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	-0.000000000041		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	-0.000000000267		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	0.000000000039		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	-0.000000000024		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	-0.000000000061		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	0.000000000044		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	-0.000000000052		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	0.000000000140		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	0.000000000103		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	0.000000000093		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	0.000000000000		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	-0.000000000039		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	0.000000000141		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	0.000000000053		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	-0.000000000074		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	-0.000000000074		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	0.000000000022		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	0.000000000126		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	0.000000000045		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.40	0.000000000001		15	34	2493.5	2099.3	0.000000004560	15	8.33	7.46	198.05	198.77	1811.43	280.41	4.31	6.73
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	0.000000000214		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000267		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	0.000000000272		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	0.000000000295		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	0.000000000199		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000122		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	0.000000000174		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	0.000000000031		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000096		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000018		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000108		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000114		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	0.000000000326		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	0.000000000056		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	0.000000000069		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000159		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000264		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000220		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000202		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	0.000000000310		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	0.000000000687		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	0.000000000551		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	0.000000000566		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	0.000000000373		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000381		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000141		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000039		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	0.000000000028		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000263		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000068		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	0.000000000095		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000168		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	0.000000000186		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000085		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000501		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000047		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000545		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000185		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000209		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000027		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000389		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	0.000000000201		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000005		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000027		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	0.000000000102		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000107		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	0.000000000105		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	0.000000000408		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000293		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000297		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	0.000000000004		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	-0.000000000251		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 8.10	0.000000000001		15	34	2309.3	2074.5	0.000000007960	15	8.09	13.50	358.43	359.73	1891.97	169.02	2.60	4.06
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000001540		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000892		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000873		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	-0.000000000515		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000313		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	-0.000000000099		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000374		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	-0.000000000134		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000001370		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000861		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000603		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000001370		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000827		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	-0.000000000080		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000469		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	-0.000000000860		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000975		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000222		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	-0.000000000255		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000112		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	-0.000000000360		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000357		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000486		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000229		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000747		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000174		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000648		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000613		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	-0.000000000883		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000001260		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000638		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000002680		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000902		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000001440		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000751		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000135		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000099		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000001330		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000001080		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000515		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000850		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000425		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	-0.000000000320		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000858		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000606		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	-0.000000000815		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000987		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000001820		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	-0.000000000146		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	-0.000000000275		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	-0.000000000325		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000001260		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000924		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000524		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000336		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000001000		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000856		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000305		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	-0.000000000026		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000961		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000224		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000002270		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000001040		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	-0.000000000488		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000561		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000087		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000490		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	-0.000000000669		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000636		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	-0.000000000948		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2e	bulk Ph 7.78	0.000000000383		15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38
laboratory	Coscinodiscus wailesii	2f	bulk ph 8		18	15	34	2206.9	2043.5	0.000000010700	15	7.95	18.88	501.25	503.06	1902.42	122.20	1.88	2.93
laboratory	Coscinodiscus wailesii		bulk Ph 7.78		122	15	34	2183.3	2059.3	0.000000013400	15	7.85	24.15	641.15	643.48	1936.19	98.96	1.52	2.38