Niedzwiedz, Sarina; Bischof, Kai (2023): Lab experiment on the effects of temperature on kelp respiration rates [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.951172
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Abstract:
Kelps act as ecosystem engineers and foundation species on many polar rocky shore coastlines. The main driver for their vertical and latitudinal distribution is the underwater light climate and temperature. Both are changing drastically in the Arctic in the course of global climate change. It was the aim of this study to analyse the effects of rising temperature and deteriorating underwater light climate on the potential habitat of kelps in the Arctic.
A laboratory experiment, in which we determined temperature-related changes in the light-use characteristics of two temperate kelp species (Alaria esculenta, Saccharina latissima) at 3, 7, and 11 °C. Therefore, grown sporophytes were sampled in the field from a sampling depth of 6–9 m. Meristematic discs (Ø 2 cm) were cut and distributed between temperature treatments and replicates. The experiment ran for seven days, during which the treatment temperature was increased every two days by 4 °C until treatment temperature was reached, allowing for successive acclimation. The photosynthesis vs. irradiance curves were measured with a 4-channel optode set-up (FireStingO2 Fibre-Optic Oxygen Meter FSO2-01, PyroScience Sensor technology, Aachen, Germany) by analysing the oxygen evolution in response to different light intensities within a 25 mL Schott bottle, each containing three meristematic discs. Maximum photosynthetic quantum yield was measured using a pulse amplitude modulated fluorometer (Portable Chlorophyll Fluorometer PAM-2100, Heinz Walz GmbH, Effeltrich, Germany). Pigment analysis was analysed with a High-Performance Liquid Chromatograph (HPLC, LaChromElite® system, L-2200 autosampler (chilled), DA-detetctor L-2450; VWR-Hitachi International GmbH, Darmstadt, Germany).
Keyword(s):
Supplement to:
Niedzwiedz, Sarina; Bischof, Kai (in review): Rising temperatures and a deteriorating light climate are limiting the expansion of temperate kelp species in the future Arctic. Limnology and Oceanography
Related to:
Niedzwiedz, Sarina; Bischof, Kai (2023): Irradiance data at different depths and sites for field sampling in the Arctic fjord Kongsfjorden. PANGAEA, https://doi.org/10.1594/PANGAEA.951173
Project(s):
Arctic Biodiversity & Livelihoods (FACE-IT)
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Type of study | Study type | Niedzwiedz, Sarina | |||
| 2 | Family | Family | Niedzwiedz, Sarina | |||
| 3 | Species | Species | Niedzwiedz, Sarina | |||
| 4 | Genus, unique identification | Genus UID | Niedzwiedz, Sarina | |||
| 5 | Genus, unique identification (URI) | Genus UID (URI) | Niedzwiedz, Sarina | |||
| 6 | Genus, unique identification (Semantic URI) | Genus UID (Semantic URI) | Niedzwiedz, Sarina | |||
| 7 | Day of experiment | DOE | day | Niedzwiedz, Sarina | Measuements of day 3, 5 and 7 of the experiment | |
| 8 | Treatment: temperature | T:temp | °C | Niedzwiedz, Sarina | Experimental temperatures in the lab: 3°C, 7°C, 11°C | |
| 9 | Deposit feeder, biomass | Depos feeder biom | % | Niedzwiedz, Sarina | Biological replicate of thre treatment: 1-4 | |
| 10 | Deposit feeder, biomass | Depos feeder biom | % | Niedzwiedz, Sarina | Technical replicate of each biologicla replicate: 1-4 | |
| 11 | Phylloid length | Phylloid l | cm | Niedzwiedz, Sarina | ||
| 12 | Phylloid width | Phylloid w | cm | Niedzwiedz, Sarina | ||
| 13 | Cauloid length | Cauloid l | cm | Niedzwiedz, Sarina | ||
| 14 | Area | Area | cm2 | Niedzwiedz, Sarina | The discs had a diameter of 2 cm. Front and back side of the discs were added up | |
| 15 | Wet mass | Wet m | g | Niedzwiedz, Sarina | Freshweight of the discs at the end of the experiment. The discs were dried with a paper towel prior to weighing | |
| 16 | Dry mass | Dry m | g | Niedzwiedz, Sarina | Dry weight of the discs at the end of each sampling. The discs were dried in Silica Gel prior to weighing | |
| 17 | Maximum photochemical quantum yield of photosystem II | Fv/Fm | Niedzwiedz, Sarina | Portable Chlorophyll Flourometer, Heinz Walz GmbH, Effeltrich, Germany, PAM-2100 | The discs were dried in Silica Gel prior to weighing | |
| 18 | Oxygen concentration at 0 µmol photons | O2 0 | µmol/l/s | Niedzwiedz, Sarina | Optical Oxygen Meter (FireSting, PyroScience GmbH, Germany) | Oxygen concentration at 0 µmol photons m-2 s-1 |
| 19 | Oxygen concentration at 0 µmol photons per time and area | O2 0 time+area | µmol/l/h/cm2 | Niedzwiedz, Sarina | Optical Oxygen Meter (FireSting, PyroScience GmbH, Germany) | Oxygen concentration at 0 µmol photons m-2 s-1 within 1 hour per cm-2 |
| 20 | Oxygen concentration at 24 µmol photons | O2 24 | µmol/l/s | Niedzwiedz, Sarina | Optical Oxygen Meter (FireSting, PyroScience GmbH, Germany) | Oxygen concentration at 24 µmol photons m-2 s-1 |
| 21 | Oxygen concentration at 24 µmol photons per time and area | O2 24 time+area | µmol/l/h/cm2 | Niedzwiedz, Sarina | Optical Oxygen Meter (FireSting, PyroScience GmbH, Germany) | Oxygen concentration at 24 µmol photons m-2 s-1 within 1 hour per cm-2 |
| 22 | Compensation point | Ec | µE/m2/s | Niedzwiedz, Sarina | RAMSES-ACC hyperspectral radiometer, TriOS | Compensation irradiance = light intensity in µmol photons m-2 s-1 at which the oxygen decline (respiration) equals the oxygen increase (photosynthesis) |
| 23 | log-compensation point | log-Ec | µE/m2/s | Niedzwiedz, Sarina | RAMSES-ACC hyperspectral radiometer, TriOS | Decadal logarithm of the compensation irradiance |
| 24 | Chlorophyll a | Chl a | µg/cm2 | Niedzwiedz, Sarina | High performance liquid chromatography (HPLC), LaChromElite (L-2200) autosampler (chilled), DA-detector L-2450 | Chlorophyll a concentration of the kelps per cm2 |
| 25 | Chlorophyll a | Chl a | µg/cm2 | Niedzwiedz, Sarina | High performance liquid chromatography (HPLC), LaChromElite (L-2200) autosampler (chilled), DA-detector L-2450 | Mean of the technical replicates of the discs chlorophyll a concentration |
| 26 | Pigments | Pigm | µg/cm2 | Niedzwiedz, Sarina | High performance liquid chromatography (HPLC), LaChromElite (L-2200) autosampler (chilled), DA-detector L-2450 | Accessory pigment (chlorophyllc, carontenoids) concentration of the kelps per cm2 |
| 27 | Pigments | Pigm | µg/cm2 | Niedzwiedz, Sarina | High performance liquid chromatography (HPLC), LaChromElite (L-2200) autosampler (chilled), DA-detector L-2450 | Mean of the technical replicates of the discs accessory pigment concentration |
| 28 | Pigments/chlorophyll a ratio | Pigm/chl a | Niedzwiedz, Sarina | Ratio of accessory pigment cocentration to chlorophyll a concentration | ||
| 29 | Pigments/chlorophyll a ratio | Pigm/chl a | Niedzwiedz, Sarina | Mean of the technical replicates of the ratio of accessory pigment concentration to chlorophyll a concentration | ||
| 30 | Nitrogen | N | % | Niedzwiedz, Sarina | Elemental analyzer, EuroVector, EuroEA 3000 | Discs nitrogen content |
| 31 | Carbon | C | % | Niedzwiedz, Sarina | Elemental analyzer, EuroVector, EuroEA 3000 | Discs carbon content |
| 32 | Carbon/Nitrogen ratio | C/N | Niedzwiedz, Sarina |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
3355 data points
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