Citation:

PANGAEA.950895

Name: Seawater carbonate chemistry and the Gd bioaccumulation and oxidative stress-related responses of the surf clam Spisula solida
Published: 2022-11-17
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Inorganic toxins; Laboratory experiment; Mollusca; North Atlantic; Other metabolic rates; Single species; Spisula solida; Temperate; Temperature

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Abstract:

Humans have exhaustively combusted fossil fuels, and released pollutants into the environment, at continuously faster rates resulting in global average temperature increase and seawater pH decrease. Climate change is forecasted to exacerbate the effects of pollutants such as the emergent rare earth elements. Therefore, the objective of this study was to assess the combined effects of rising temperature (delta = + 4 °C) and decreasing pH (delta = − 0.4 pH units) on the bioaccumulation and elimination of gadolinium (Gd) in the bioindicator bivalve species Spisula solida (Surf clam). We exposed surf clams to 10 µg/L of GdCl3 for seven days, under warming, acidification, and their combination, followed by a depuration phase lasting for another 7 days and investigated the Gd bioaccumulation and oxidative stress-related responses after 1, 3 and 7 days of exposure and the elimination phase. Gadolinium accumulated after just one day with values reaching the highest after 7 days. Gadolinium was not eliminated after 7 days, and elimination is further hampered under climate change scenarios. Warming and acidification, and their interaction did not significantly impact Gd concentration. However, there was a significant interaction on clam's biochemical response. The augmented total antioxidant capacity and lipid peroxidation values show that the significant impacts of Gd on the oxidative stress response are enhanced under warming while the increased superoxide dismutase and catalase values demonstrate the combined impact of Gd, warming & acidification. Ultimately, lipid damage was greater in clams exposed to warming & Gd, which emphasizes the enhanced toxic effects of Gd in a changing ocean.

Keyword(s):

Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Inorganic toxins; Laboratory experiment; Mollusca; North Atlantic; Other metabolic rates; Single species; Spisula solida; Temperate; Temperature

Supplement to:

Figueiredo, Catia; Grilo, Tiago F; Oliveira, Rui; Ferreira, Ines Joao; Gil, Fatima; Lopes, C; Brito, Pedro; Ré, P; Caetano, Miguel; Diniz, Mário; Raimundo, Joana (2022): Gadolinium ecotoxicity is enhanced in a warmer and acidified changing ocean as shown by the surf clam Spisula solida through a multibiomarker approach. Aquatic Toxicology, 253, 106346, https://doi.org/10.1016/j.aquatox.2022.106346

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-11-16.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Figueiredo, Catia		Study
2	Species, unique identification	Species UID		Figueiredo, Catia
3	Species, unique identification (URI)	Species UID (URI)		Figueiredo, Catia
4	Species, unique identification (Semantic URI)	Species UID (Semantic URI)		Figueiredo, Catia
5	Treatment	Treat		Figueiredo, Catia
6	Sampling date	Sampling date		Figueiredo, Catia
7	Cadmium	Cd	µg/g	Figueiredo, Catia		per dry mass, in the whole soft body of clams
8	Cadmium, error	Cd e	±	Figueiredo, Catia
9	Lipid peroxidation, per protein	LPO/prot	nmol/mg	Figueiredo, Catia
10	Lipid peroxidation, standard deviation	LPO std dev	±	Figueiredo, Catia
11	Total antioxidant capacity, per protein mass	TAC/prot	mmol/mg	Figueiredo, Catia
12	Total antioxidant capacity, per protein mass, standard deviation	TAC/prot std dev	±	Figueiredo, Catia
13	Superoxide dismutase activity, inhibition, per protein mass	SOD inhib/prot	%/min/mg	Figueiredo, Catia
14	Superoxide dismutase activity, standard deviation	SOD std dev	±	Figueiredo, Catia
15	Catalase activity, per protein mass	CAT/prot	nmol/min/mg	Figueiredo, Catia
16	Catalase activity, standard deviation	CAT std dev	±	Figueiredo, Catia
17	Total glutathione peroxidases activity, per protein mass	GPxTOT/prot	nmol/min/mg	Figueiredo, Catia
18	Total glutathione peroxidases activity, unit per protein mass, standard deviation	GPxTOT std dev	±	Figueiredo, Catia
19	Glutathione S-transferase, activity per protein mass	GST/prot	nmol/min/mg	Figueiredo, Catia
20	Glutathione S-transferase, activity per protein mass, standard deviation	GST std dev	±	Figueiredo, Catia
21	Heat shock protein, per protein mass	HSP/prot	ng/mg	Figueiredo, Catia
22	Heat shock protein, per protein mass, standard deviation	HSP/prot std dev	±	Figueiredo, Catia
23	Ubiquitin	Ub	µg/mg	Figueiredo, Catia
24	Ubiquitin, standard deviation	Ub std dev	±	Figueiredo, Catia
25	Individuals	Ind	#	Figueiredo, Catia
26	Temperature, water	Temp	°C	Figueiredo, Catia
27	Temperature, water, standard deviation	Temp std dev	±	Figueiredo, Catia
28	Salinity	Sal		Figueiredo, Catia
29	Salinity, standard deviation	Sal std dev	±	Figueiredo, Catia
30	pH	pH		Figueiredo, Catia	Potentiometric	total scale
31	pH, standard deviation	pH std dev	±	Figueiredo, Catia	Potentiometric	total scale
32	Alkalinity, total	AT	µmol/kg	Figueiredo, Catia	Potentiometric titration
33	Alkalinity, total, standard deviation	AT std dev	±	Figueiredo, Catia	Potentiometric titration
34	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Figueiredo, Catia	Calculated using CO2SYS
35	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Figueiredo, Catia	Calculated using CO2SYS
36	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
40	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

2472 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (Study)	2 Species UID	3 Species UID (URI)	4 Species UID (Semantic URI)	5 Treat	6 Sampling date	7 Cd [µg/g] (per dry mass, in the whole so...)	8 Cd e [±]	9 LPO/prot [nmol/mg]	10 LPO std dev [±]	11 TAC/prot [mmol/mg]	12 TAC/prot std dev [±]	13 SOD inhib/prot [%/min/mg]	14 SOD std dev [±]	15 CAT/prot [nmol/min/mg]	16 CAT std dev [±]	17 GPxTOT/prot [nmol/min/mg]	18 GPxTOT std dev [±]	19 GST/prot [nmol/min/mg]	20 GST std dev [±]	21 HSP/prot [ng/mg]	22 HSP/prot std dev [±]	23 Ub [µg/mg]	24 Ub std dev [±]	25 Ind [#]	26 Temp [°C]	27 Temp std dev [±]	28 Sal	29 Sal std dev [±]	30 pH (total scale, Potentiometric)	31 pH std dev [±] (total scale, Potentiometric)	32 AT [µmol/kg] (Potentiometric titration)	33 AT std dev [±] (Potentiometric titration)	34 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	35 pCO2 std dev [±] (Calculated using CO2SYS)	36 CSC flag (Calculated using seacarb afte...)	37 CO2 [µmol/kg] (Calculated using seacarb afte...)	38 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	39 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	40 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	41 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	42 AT [µmol/kg] (Calculated using seacarb afte...)	43 Omega Arg (Calculated using seacarb afte...)	44 Omega Cal (Calculated using seacarb afte...)
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Control	0	0.070	0.011																	40	15	0.2	35	0.1	8.0	0.10	1928	109	367	67	8	14.09	376.22	377.58	1618.20	120.23	1752.52	1.84	2.87
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Acidification	0	0.069	0.009																	40	15	0.3	35	0.1	7.6	0.10	1643	64	893	73	8	33.31	889.13	892.35	1522.49	45.04	1600.83	0.69	1.07
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Gd exposed	0																			32	15	0.2	35	0.1	8.1	0.04	2536	43	372	28	8	14.29	381.47	382.86	2065.64	193.22	2273.15	2.96	4.61
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Acidification and Gd	0																			32	15	0.1	35	0.1	7.6	0.09	1769	108	894	35	8	35.91	958.60	962.08	1641.46	48.55	1725.92	0.74	1.16
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming	0	0.073	0.004																	40	19	0.2	35	0.1	8.0	0.06	2307	102	442	65	8	15.13	453.88	455.45	1901.09	164.48	2080.70	2.55	3.93
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming and acidification	0	0.080	0.002																	40	19	0.3	35	0.1	7.6	0.14	1550	83	812	22	8	28.34	850.52	853.45	1418.22	48.85	1495.41	0.76	1.17
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming and Gd	0																			32	19	0.1	35	0.1	8.1	0.04	2206	69	338	38	8	10.97	329.07	330.20	1735.18	189.00	1935.14	2.93	4.52
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming, acidification and Gd	0																			32	19	0.2	35	0.1	7.6	0.10	1671	95	834	28	8	30.61	918.41	921.58	1531.42	52.75	1614.77	0.82	1.26
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Control	1	0.098	0.007																	40	15	0.2	35	0.1	8.0	0.10	1928	109	367	67	8	14.09	376.22	377.58	1618.20	120.23	1752.52	1.84	2.87
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Acidification	1	0.099	0.008																	40	15	0.3	35	0.1	7.6	0.10	1643	64	893	73	8	33.31	889.13	892.35	1522.49	45.04	1600.83	0.69	1.07
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Gd exposed	1	1.000	0.110																	32	15	0.2	35	0.1	8.1	0.04	2536	43	372	28	8	14.29	381.47	382.86	2065.64	193.22	2273.15	2.96	4.61
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Acidification and Gd	1	1.800	0.460																	32	15	0.1	35	0.1	7.6	0.09	1769	108	894	35	8	35.91	958.60	962.08	1641.46	48.55	1725.92	0.74	1.16
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming	1	0.068	0.016																	40	19	0.2	35	0.1	8.0	0.06	2307	102	442	65	8	15.13	453.88	455.45	1901.09	164.48	2080.70	2.55	3.93
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming and acidification	1	0.076	0.011																	40	19	0.3	35	0.1	7.6	0.14	1550	83	812	22	8	28.34	850.52	853.45	1418.22	48.85	1495.41	0.76	1.17
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming and Gd	1	1.700	0.250																	32	19	0.1	35	0.1	8.1	0.04	2206	69	338	38	8	10.97	329.07	330.20	1735.18	189.00	1935.14	2.93	4.52
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming, acidification and Gd	1	2.000	0.300																	32	19	0.2	35	0.1	7.6	0.10	1671	95	834	28	8	30.61	918.41	921.58	1531.42	52.75	1614.77	0.82	1.26
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Control	3	0.071	0.013																	40	15	0.2	35	0.1	8.0	0.10	1928	109	367	67	8	14.09	376.22	377.58	1618.20	120.23	1752.52	1.84	2.87
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Acidification	3	0.086	0.004																	40	15	0.3	35	0.1	7.6	0.10	1643	64	893	73	8	33.31	889.13	892.35	1522.49	45.04	1600.83	0.69	1.07
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Gd exposed	3	2.600	0.720																	32	15	0.2	35	0.1	8.1	0.04	2536	43	372	28	8	14.29	381.47	382.86	2065.64	193.22	2273.15	2.96	4.61
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Acidification and Gd	3	3.100	0.720																	32	15	0.1	35	0.1	7.6	0.09	1769	108	894	35	8	35.91	958.60	962.08	1641.46	48.55	1725.92	0.74	1.16
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming	3	0.140	0.008																	40	19	0.2	35	0.1	8.0	0.06	2307	102	442	65	8	15.13	453.88	455.45	1901.09	164.48	2080.70	2.55	3.93
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming and acidification	3	0.071	0.018																	40	19	0.3	35	0.1	7.6	0.14	1550	83	812	22	8	28.34	850.52	853.45	1418.22	48.85	1495.41	0.76	1.17
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming and Gd	3	2.500	0.570																	32	19	0.1	35	0.1	8.1	0.04	2206	69	338	38	8	10.97	329.07	330.20	1735.18	189.00	1935.14	2.93	4.52
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming, acidification and Gd	3	2.700	0.120																	32	19	0.2	35	0.1	7.6	0.10	1671	95	834	28	8	30.61	918.41	921.58	1531.42	52.75	1614.77	0.82	1.26
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Control	7	0.098	0.002																	40	15	0.2	35	0.1	8.0	0.10	1928	109	367	67	8	14.09	376.22	377.58	1618.20	120.23	1752.52	1.84	2.87
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Acidification	7	0.110	0.004																	40	15	0.3	35	0.1	7.6	0.10	1643	64	893	73	8	33.31	889.13	892.35	1522.49	45.04	1600.83	0.69	1.07
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Gd exposed	7	2.700	0.051																	32	15	0.2	35	0.1	8.1	0.04	2536	43	372	28	8	14.29	381.47	382.86	2065.64	193.22	2273.15	2.96	4.61
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Acidification and Gd	7	4.100	0.370																	32	15	0.1	35	0.1	7.6	0.09	1769	108	894	35	8	35.91	958.60	962.08	1641.46	48.55	1725.92	0.74	1.16
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming	7	0.120	0.024																	40	19	0.2	35	0.1	8.0	0.06	2307	102	442	65	8	15.13	453.88	455.45	1901.09	164.48	2080.70	2.55	3.93
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming and acidification	7	0.410	0.170																	40	19	0.3	35	0.1	7.6	0.14	1550	83	812	22	8	28.34	850.52	853.45	1418.22	48.85	1495.41	0.76	1.17
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming and Gd	7	4.200	0.820																	32	19	0.1	35	0.1	8.1	0.04	2206	69	338	38	8	10.97	329.07	330.20	1735.18	189.00	1935.14	2.93	4.52
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming, acidification and Gd	7	3.000	0.210																	32	19	0.2	35	0.1	7.6	0.10	1671	95	834	28	8	30.61	918.41	921.58	1531.42	52.75	1614.77	0.82	1.26
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Control	14	0.084	0.008																	40	15	0.2	35	0.1	8.0	0.10	1928	109	367	67	8	14.09	376.22	377.58	1618.20	120.23	1752.52	1.84	2.87
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Acidification	14	0.099	0.017																	40	15	0.3	35	0.1	7.6	0.10	1643	64	893	73	8	33.31	889.13	892.35	1522.49	45.04	1600.83	0.69	1.07
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Gd exposed	14	2.100	0.300																	32	15	0.2	35	0.1	8.1	0.04	2536	43	372	28	8	14.29	381.47	382.86	2065.64	193.22	2273.15	2.96	4.61
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Acidification and Gd	14	2.100	0.300																	32	15	0.1	35	0.1	7.6	0.09	1769	108	894	35	8	35.91	958.60	962.08	1641.46	48.55	1725.92	0.74	1.16
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming	14	0.170	0.007																	40	19	0.2	35	0.1	8.0	0.06	2307	102	442	65	8	15.13	453.88	455.45	1901.09	164.48	2080.70	2.55	3.93
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming and acidification	14	0.150	0.022																	40	19	0.3	35	0.1	7.6	0.14	1550	83	812	22	8	28.34	850.52	853.45	1418.22	48.85	1495.41	0.76	1.17
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming and Gd	14	2.000	0.360																	32	19	0.1	35	0.1	8.1	0.04	2206	69	338	38	8	10.97	329.07	330.20	1735.18	189.00	1935.14	2.93	4.52
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming, acidification and Gd	14	1.900	0.210																	32	19	0.2	35	0.1	7.6	0.10	1671	95	834	28	8	30.61	918.41	921.58	1531.42	52.75	1614.77	0.82	1.26
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Control	0			4.5	0.47	0.73	0.0500	5.5	0.71	1.1	0.34	5.2	0.65	30	4.3	54000	4800	1.8	0.24	40	15	0.2	35	0.1	8.0	0.10	1928	109	367	67	8	14.09	376.22	377.58	1618.20	120.23	1752.52	1.84	2.87
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Acidification	0			6.2	2.90	0.83	0.1100	4.5	1.60	1.2	0.39	5.8	0.89	35	3.3	58000	5300	1.9	0.87	40	15	0.3	35	0.1	7.6	0.10	1643	64	893	73	8	33.31	889.13	892.35	1522.49	45.04	1600.83	0.69	1.07
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming	0			5.7	0.74	0.84	0.0100	5.1	1.30	0.9	0.16	6.0	1.65	34	4.3	60000	5400	1.8	0.03	40	19	0.2	35	0.1	8.0	0.06	2307	102	442	65	8	15.13	453.88	455.45	1901.09	164.48	2080.70	2.55	3.93
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming and acidification	0			5.1	0.83	0.74	0.1500	5.6	0.69	1.1	0.35	5.6	1.12	38	2.2	54000	2700	2.4	0.27	40	19	0.3	35	0.1	7.6	0.14	1550	83	812	22	8	28.34	850.52	853.45	1418.22	48.85	1495.41	0.76	1.17
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Control	1			5.6	1.20	0.78	0.0700	5.7	0.66	1.2	0.29	5.1	1.20	30	3.3	48000	5600	1.6	1.10	40	15	0.2	35	0.1	8.0	0.10	1928	109	367	67	8	14.09	376.22	377.58	1618.20	120.23	1752.52	1.84	2.87
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Gd exposed	1			4.8	1.10	0.74	0.2200	5.4	0.95	1.1	0.10	6.4	0.68	42	1.3	57000	9300	1.5	0.61	32	15	0.2	35	0.1	8.1	0.04	2536	43	372	28	8	14.29	381.47	382.86	2065.64	193.22	2273.15	2.96	4.61
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Acidification	1			4.9	2.50	0.80	0.0100	4.6	0.34	1.4	0.02	5.9	0.21	33	2.9	56000	11000	2.1	0.90	40	15	0.3	35	0.1	7.6	0.10	1643	64	893	73	8	33.31	889.13	892.35	1522.49	45.04	1600.83	0.69	1.07
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Acidification and Gd	1			5.4	0.73	0.74	0.0400	5.6	0.71	0.9	0.39	5.4	0.32	44	6.7	55000	1700	2.0	0.60	32	15	0.1	35	0.1	7.6	0.09	1769	108	894	35	8	35.91	958.60	962.08	1641.46	48.55	1725.92	0.74	1.16
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming	1			6.1	1.70	0.71	0.0700	5.6	0.58	0.8	0.03	5.7	0.34	32	1.4	56000	7100	1.9	0.78	40	19	0.2	35	0.1	8.0	0.06	2307	102	442	65	8	15.13	453.88	455.45	1901.09	164.48	2080.70	2.55	3.93
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming and Gd	1			3.4	1.20	1.90	0.2700	4.7	1.00	1.3	0.11	5.6	1.10	43	5.0	44000	400	1.3	0.08	32	19	0.1	35	0.1	8.1	0.04	2206	69	338	38	8	10.97	329.07	330.20	1735.18	189.00	1935.14	2.93	4.52
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming and acidification	1			4.9	1.10	0.77	0.3500	6.4	1.20	1.0	0.31	5.7	1.90	38	4.0	46000	8700	2.4	0.49	40	19	0.3	35	0.1	7.6	0.14	1550	83	812	22	8	28.34	850.52	853.45	1418.22	48.85	1495.41	0.76	1.17
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming, acidification and Gd	1			2.9	0.27	1.70	0.0300	6.2	0.92	1.4	0.32	4.8	0.28	48	6.5	48000	9200	1.7	0.45	32	19	0.2	35	0.1	7.6	0.10	1671	95	834	28	8	30.61	918.41	921.58	1531.42	52.75	1614.77	0.82	1.26
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Control	3			2.9	0.88	1.10	0.0001	5.5	0.15	1.1	0.04	4.6	0.24	27	1.9	45000	9300	2.0	1.40	40	15	0.2	35	0.1	8.0	0.10	1928	109	367	67	8	14.09	376.22	377.58	1618.20	120.23	1752.52	1.84	2.87
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Gd exposed	3			2.4	0.77	1.80	0.2800	5.1	0.43	1.0	0.02	3.5	0.29	36	3.7	30000	7700	2.7	0.35	32	15	0.2	35	0.1	8.1	0.04	2536	43	372	28	8	14.29	381.47	382.86	2065.64	193.22	2273.15	2.96	4.61
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Acidification	3			2.8	0.89	1.20	0.3000	4.9	1.00	1.4	0.02	3.6	0.56	30	4.1	46000	6200	2.6	0.65	40	15	0.3	35	0.1	7.6	0.10	1643	64	893	73	8	33.31	889.13	892.35	1522.49	45.04	1600.83	0.69	1.07
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Acidification and Gd	3			3.6	0.68	1.90	0.1800	6.1	0.61	1.2	0.08	3.2	0.23	42	4.3	44000	2500	3.9	0.77	32	15	0.1	35	0.1	7.6	0.09	1769	108	894	35	8	35.91	958.60	962.08	1641.46	48.55	1725.92	0.74	1.16
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming	3			3.4	0.47	1.70	0.1500	6.9	0.68	0.9	0.10	4.7	0.22	29	3.4	46000	8900	2.2	0.67	40	19	0.2	35	0.1	8.0	0.06	2307	102	442	65	8	15.13	453.88	455.45	1901.09	164.48	2080.70	2.55	3.93
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming and Gd	3			3.1	1.90	2.40	0.1800	5.7	2.20	1.1	0.09	4.6	0.36	40	4.4	46000	900	2.7	1.10	32	19	0.1	35	0.1	8.1	0.04	2206	69	338	38	8	10.97	329.07	330.20	1735.18	189.00	1935.14	2.93	4.52
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming and acidification	3			3.3	0.53	1.40	0.3300	6.3	2.00	1.0	0.10	4.1	0.46	33	5.5	37000	8900	2.2	0.10	40	19	0.3	35	0.1	7.6	0.14	1550	83	812	22	8	28.34	850.52	853.45	1418.22	48.85	1495.41	0.76	1.17
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming, acidification and Gd	3			2.6	0.08	2.40	0.3200	6.0	1.10	1.6	0.02	4.4	0.32	43	1.3	27000	4200	3.3	0.18	32	19	0.2	35	0.1	7.6	0.10	1671	95	834	28	8	30.61	918.41	921.58	1531.42	52.75	1614.77	0.82	1.26
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Control	7			3.8	0.33	1.40	0.0600	4.6	1.00	1.4	0.03	4.1	0.40	31	2.6	41000	7400	1.4	0.65	40	15	0.2	35	0.1	8.0	0.10	1928	109	367	67	8	14.09	376.22	377.58	1618.20	120.23	1752.52	1.84	2.87
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Gd exposed	7			8.3	2.40	1.80	0.1700	6.1	1.00	1.5	0.14	2.1	0.30	22	3.9	18000	8500	0.4	0.17	32	15	0.2	35	0.1	8.1	0.04	2536	43	372	28	8	14.29	381.47	382.86	2065.64	193.22	2273.15	2.96	4.61
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Acidification	7			4.2	0.71	1.60	0.0200	4.0	0.84	2.1	0.07	3.0	0.63	31	3.3	44000	8300	2.3	1.20	40	15	0.3	35	0.1	7.6	0.10	1643	64	893	73	8	33.31	889.13	892.35	1522.49	45.04	1600.83	0.69	1.07
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Acidification and Gd	7			9.0	2.00	2.10	0.2900	5.8	0.39	1.9	0.14	2.4	0.23	21	4.1	23000	7300	0.9	0.34	32	15	0.1	35	0.1	7.6	0.09	1769	108	894	35	8	35.91	958.60	962.08	1641.46	48.55	1725.92	0.74	1.16
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming	7			12.0	2.30	3.30	0.4700	8.2	0.89	2.1	0.20	3.8	0.61	31	2.6	54000	12000	2.8	0.16	40	19	0.2	35	0.1	8.0	0.06	2307	102	442	65	8	15.13	453.88	455.45	1901.09	164.48	2080.70	2.55	3.93
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming and Gd	7			13.0	2.70	3.00	0.7400	7.1	0.63	2.0	0.14	2.5	0.95	23	2.7	43000	500	0.8	0.17	32	19	0.1	35	0.1	8.1	0.04	2206	69	338	38	8	10.97	329.07	330.20	1735.18	189.00	1935.14	2.93	4.52
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming and acidification	7			14.0	3.40	2.70	0.1800	7.1	0.43	2.0	0.17	2.5	0.31	30	5.5	32000	3900	4.2	0.19	40	19	0.3	35	0.1	7.6	0.14	1550	83	812	22	8	28.34	850.52	853.45	1418.22	48.85	1495.41	0.76	1.17
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming, acidification and Gd	7			9.5	0.97	2.50	0.0800	8.1	0.58	2.2	0.12	2.1	0.80	24	2.3	31000	9300	1.0	0.01	32	19	0.2	35	0.1	7.6	0.10	1671	95	834	28	8	30.61	918.41	921.58	1531.42	52.75	1614.77	0.82	1.26
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Control	14			3.5	0.20	1.30	0.3000	6.0	0.47	1.4	0.18	3.5	0.36	30	4.8	33000	4900	1.7	0.18	40	15	0.2	35	0.1	8.0	0.10	1928	109	367	67	8	14.09	376.22	377.58	1618.20	120.23	1752.52	1.84	2.87
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Gd exposed	14			3.4	0.56	1.90	0.6400	8.3	0.37	1.5	0.26	3.8	0.64	41	6.0	24000	2600	0.3	0.04	32	15	0.2	35	0.1	8.1	0.04	2536	43	372	28	8	14.29	381.47	382.86	2065.64	193.22	2273.15	2.96	4.61
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Acidification	14			2.6	0.38	1.70	0.2300	6.6	0.97	1.8	0.12	3.4	0.31	30	4.1	41000	1300	1.7	0.28	40	15	0.3	35	0.1	7.6	0.10	1643	64	893	73	8	33.31	889.13	892.35	1522.49	45.04	1600.83	0.69	1.07
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Acidification and Gd	14			3.5	0.10	2.10	0.4200	8.0	0.06	1.6	0.02	4.1	0.64	42	7.6	33000	4500	1.1	0.10	32	15	0.1	35	0.1	7.6	0.09	1769	108	894	35	8	35.91	958.60	962.08	1641.46	48.55	1725.92	0.74	1.16
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming	14			7.6	0.38	3.20	0.4000	7.7	0.49	1.9	0.11	3.7	0.40	29	3.4	47000	9700	1.0	0.12	40	19	0.2	35	0.1	8.0	0.06	2307	102	442	65	8	15.13	453.88	455.45	1901.09	164.48	2080.70	2.55	3.93
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming and Gd	14			4.6	1.80	2.80	0.6800	7.7	1.60	1.6	0.05	2.2	0.27	33	7.2	32000	9000	1.3	0.28	32	19	0.1	35	0.1	8.1	0.04	2206	69	338	38	8	10.97	329.07	330.20	1735.18	189.00	1935.14	2.93	4.52
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming and acidification	14			7.0	1.10	2.20	0.1100	8.0	0.02	2.2	0.17	3.0	1.10	32	0.3	43000	9200	3.7	0.46	40	19	0.3	35	0.1	7.6	0.14	1550	83	812	22	8	28.34	850.52	853.45	1418.22	48.85	1495.41	0.76	1.17
laboratory	Spisula solida	marinespecies.org	urn:lsid:marinespecies.org:taxname:140301	Warming, acidification and Gd	14			2.6	0.07	1.70	0.0500	7.8	1.10	1.7	0.01	2.4	0.11	33	5.1	27000	8400	2.3	0.79	32	19	0.2	35	0.1	7.6	0.10	1671	95	834	28	8	30.61	918.41	921.58	1531.42	52.75	1614.77	0.82	1.26